David. Reichmuth

Sandia National Laboratories California

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Brian J. Kirby

Cornell University

Jay D. Keasling

Lawrence Berkeley National Laboratory

Harvey W. Blanch

University of California, Riverside

Todd H. West

Sandia National Laboratories

Jay O. Keller

Carbon Solutions (United States)

Dawn Kataoka Manley

Sandia National Laboratories California

Timothy J. Shepodd

Lawrence Livermore National Security

Garrett Barter

National Renewable Energy Laboratory

Rajat Sapra

Indian Institute of Technology Kanpur

Thomas E. Drennen

Hobart and William Smith Colleges

Cooperative Institutions

Sandia National Laboratories California

Sandia National Laboratories

National Technical Information Service

Office of Scientific and Technical Information

University of California, Berkeley

Lawrence Berkeley National Laboratory

Pacific Northwest National Laboratory

Lawrence Livermore National Security

Oak Ridge National Laboratory

Joint BioEnergy Institute

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Biodesulfurization of dibenzothiophene in Escherichia coli is enhanced by expression of a Vibrio harveyi oxidoreductase gene

Biotechnology and Bioengineering (2000)

David. Reichmuth Jessica L. Hittle Harvey W. Blanch Jay D. Keasling

One possible alternative to current fuel hydrodesulfurization methods is the use of microorganisms to remove sulfur compounds. Biodesulfurization requires much milder processing conditions, gives higher specificity, and does not require molecular hydrogen. In the present work we have produced two compatible plasmids: pDSR3, which allows Escherichia coli to convert dibenzothiophene (DBT) to hydroxybiphenyl (HBP), and pDSR2, which produces a Vibrio harveyi flavin oxidoreductase. We show that the flavin oxidoreductase enhances the rate of DBT removal when co-expressed in vivo with the desulfurization enzymes. The plasmids pDSR2 and pDSR3 were co-expressed in growing cultures. The expression of oxidoreductase caused an increase in the rate of DBT removal but a decrease in the rate of HBP production. The maximum rate of DBT removal was 8 mg/h · g dry cell weight. Experiments were also conducted using resting cells with the addition of various carbon sources. It was found that the addition of glucose or glycerol to cultures with oxidoreductase expression produced the highest DBT removal rate (51 mg/h · g dry cell weight). The culture with acetate and no oxidoreductase expression had the highest level of HBP production. For all carbon sources, the DBT removal rate was faster and the HBP generation rate slower with the expression of the oxidoreductase. Analysis of desulfurization intermediates indicates that the last enzyme in the pathway may be limiting. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 67: 72–79, 2000.

Dibenzothiophene

10.1002/(sici)1097-0290(20000105)67:1<72::aid-bit8>3.3.co;2-3

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Comparison of the technical potential for hydrogen, battery electric, and conventional light-duty vehicles to reduce greenhouse gas emissions and petroleum consumption in the United States

International Journal of Hydrogen Energy (2012)

David. Reichmuth Andrew E. Lutz Dawn Kataoka Manley Jay O. Keller

Battery electric vehicle

Hydrogen vehicle

10.1016/j.ijhydene.2012.10.047

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Citations (47)

Analysis of biomolecules using an integrated HPLC/MS microchip.

Timothy J. Shepodd Brian J. Kirby David. Reichmuth

Biomolecule

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Parametric analysis of technology and policy tradeoffs for conventional and electric light-duty vehicles

Energy Policy (2012)

Garrett Barter David. Reichmuth Jessica Westbrook Leonard Malczynski Todd H. West

Market Penetration

Payback period

Baseline (sea)

10.1016/j.enpol.2012.04.013

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Citations (28)

The Future Adoption and Benefit of Electric Vehicles: A Parametric Assessment.

OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (2013)

Garrett Barter David. Reichmuth Todd H. West Dawn Kataoka Manley

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Impact of Hydrogen Vehicles on U.S. Greenhouse Gas Emissions and Petroleum Use.

OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (2011)

David. Reichmuth Thomas E. Drennen Jay O. Keller

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Microfluidic routing of aqueous and organic flows at high pressures: fabrication and characterization of integrated polymer microvalve elements

Lab on a Chip (2004)

Brian J. Kirby David. Reichmuth Ronald F. Renzi Timothy J. Shepodd B. Wiedenman

This paper presents the first systematic engineering study of the impact of chemical formulation and surface functionalization on the performace of free-standing microfluidic polymer elements used for high-pressure fluid control in glass microsystems. System design, chemical wet-etch processes, and laser-induced polymerization techniques are described, and parametric studies illustrate the effects of polymer formulation, glass surface modification, and geometric constraints on system performance parameters. In particular, this study shows that highly crosslinked and fluorinated polymers can overcome deficiencies in previously-reported microvalve architectures, particularly limited solvent compatibility. Substrate surface modification is shown effective in reducing the friction of the polymer-glass interface and thereby facilitating valve actuation. A microchip one-way valve constructed using this architecture shows a 2 x 10(8) ratio of forward and backward flow rates at 7 MPa. This valve architecture is integrated on chip with minimal dead volumes (70 pl), and should be applicable to systems (including chromatography and chemical synthesis devices) requiring high pressures and solvents of varying polarity.

Surface Modification

Fluidics

10.1039/b413199a

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Citations (29)

Analysis of peptides using an integrated microchip HPLC-MS/MS system.

Brian J. Kirby Gabriela S. Chirica David. Reichmuth

Hyphendated LC-MS techniques are quickly becoming the standard tool for protemic analyses. For large homogeneous samples, bulk processing methods and capillary injection and separation techniques are suitable. However, for analysis of small or heterogeneous samples, techniques that can manipulate picoliter samples without dilution are required or samples will be lost or corrupted; further, static nanospray-type flowrates are required to maximize SNR. Microchip-level integration of sample injection with separation and mass spectrometry allow small-volume analytes to be processed on chip and immediately injected without dilution for analysis. An on-chip HPLC was fabricated using in situ polymerization of both fixed and mobile polymer monoliths. Integration of the chip with a nanospray MS emitter enables identification of peptides by the use of tandem MS. The chip is capable of analyzing of very small sample volumes (< 200 pl) in short times (< 3 min).

Dilution

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Parametric analysis of technology and policy tradeoffs for conventional and electric lightduty vehicles.

OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (2012)

Dawn Kataoka Manley Garrett Barter David. Reichmuth Jessica Westbrook Leonard Malczynski

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Hydrogen Resource Analysis Update HIA Task 30 .

OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (2012)

Thomas E. Drennen David. Reichmuth Todd H. West Susan Schoenung

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