Naoki Koide

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Combination of epidermal growth factor and insulin is required for multicellular spheroid formation of rat hepatocytes in primary culture.

PubMed (1992)

Mitsuhiko Kawaguchi Naoki Koide Kousaku Sakaguchi Toshiyuki Shinji Toshiya Tsuji

We showed that the combination of epidermal growth factor (EGF) and insulin is an essential supplement to Williams' #E medium for the formation of floating multicellular spheroids in primary culture of rat hepatocytes. Isolated hepatocytes assembled to form floating multicellular spheroids within 96 h through transient assembly of monolayer islands within the initial 24 h in dishes coated with liver-derived proteoglycans. However, the assembly of multicellular spheroids was severely suppressed in the absence of either EGF or insulin. The reduction of spheroid assembly was correlated with decreased attachment and subsequent decreased formation of monolayer islands within 24 h. The minimum amounts of EGF and insulin required for the formation of floating spheroids were 1 ng/ml and 0.4 microgram/ml, respectively. These results suggest that the enhancement of hepatocyte attachment provided by the combination of EGF and insulin during the early phase of culture is required for the formation of floating spheroids.

Spheroid

Multicellular organism

10.18926/amo/32674

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Citations (14)

The Inhibitory Action of Sodium Arsenite on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 267.4 Macrophage Cells: A Role of Raf-1 in Lipopolysaccharide Signaling

The Journal of Immunology (2001)

Dipshikha Chakravortty Yutaka Kato Tsuyoshi Sugiyama Naoki Koide Mya Mya Mu

Abstract The effect of sodium arsenite (SA) on LPS-induced NO production in RAW 267.4 murine macrophage cells was studied. SA pretreatment of LPS-stimulated RAW cells resulted in a striking reduction in NO production. No significant difference in LPS binding was observed between RAW cells pretreated with SA and control untreated RAW cells, suggesting that SA might impair the intracellular signal pathway for NO production. SA inhibited LPS-induced NF-κB activation by preventing loss of IκB-α and -β. Furthermore, SA blocked phosphorylation of extracellular signal-regulated kinase 1/2 (Erk1/2), but not phosphorylation of p38 and c-Jun N-terminal kinase. SA treatment resulted in the disappearance of Raf-1, suggesting that it might cause the inhibition of the Erk1/2 mitogen-activated protein (MAP) kinase pathway. The SA-mediated loss of Raf-1 also abolished LPS-induced NF-κB activation as well as the Erk1/2 pathway. The dominant negative mutant of MAP kinase kinase 1 inhibited both NO production and NF-κB activation in LPS-stimulated RAW cells. Taken together, these results indicate that the inhibitory action of SA on NO production in LPS-stimulated macrophages might be due to abrogation of inducible NO synthase induction, and it might be closely related to inactivation of the NF-κB and Erk1/2 MAP kinase pathways through loss of Raf-1.

Sodium arsenite

10.4049/jimmunol.166.3.2011

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Citations (57)

Role of tumor necrosis factor-α and glucocorticoid on lipopolysaccharide (LPS)-induced apoptosis of thymocytes

FEMS Immunology & Medical Microbiology (1995)

Yoshiro Kato Akiko Morikawa Tsuyoshi Sugiyama Naoki Koide G-Z. Jiang

Administration of bacterial lipopolysaccharide (LPS) into mice markedly induced the apoptosis of CD4+8+ thymocytes. The injection of anti-tumor necrosis factor (TNF)-alpha antibody or RU38486, a glucocorticoid receptor antagonist, into mice definitely inhibited LPS-induced apoptosis of thymocytes. Addition of the sera 1 h after injection of LPS into in vitro cultures of thymocytes caused thymocyte apoptosis. It was also prevented by either anti-TNF-alpha antibody or RU38486. Further, recombinant TNF-alpha and hydrocortisone collaborated in induction of the thymocyte apoptosis in vitro. The in vivo phenomenon of LPS-induced apoptosis of thymocytes was reproducible by the in vitro experimental system. It was therefore suggested that both TNF-alpha and glucocorticoid participate and collaborate as effector molecules in LPS-induced apoptosis of thymocytes.

Thymocyte

10.1111/j.1574-695x.1995.tb00192.x

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Citations (52)

Butyrate enhances the production of nitric oxide in mouse vascular endothelial cells in response to gamma interferon

Journal of Endotoxin Research (2004)

Akiko Morikawa Tsuyoshi Sugiyama Naoki Koide Isamu Mori Mya Mya Mu

The effect of butyrate, a natural bacterial product of colonic bacterial flora, on nitric oxide (NO) production in murine vascular endothelial cell line END-D in response to IFN-γ and/or LPS was studied. Butyrate significantly augmented NO production in END-D cells in response to IFN-γ or IFN-γ + LPS, but not LPS alone. The NO production was augmented by the addition of butyrate until 6 h after the stimulation with IFN-γ or IFN-γ + LPS. The augmentation was abolished by the removal of butyrate from the cultures. Butyrate enhanced the expression of inducible type NO synthase (iNOS) in the stimulated END-D cells. Furthermore, butyrate-enhanced NO production in the presence of various signal inhibitors down-regulating the signal pathways using nuclear factor (NF)-κB, mitogen-activated protein (MAP) kinases and Janus tyrosine kinase. The putative mechanism of butyrate-induced augmentation of NO production in response to IFN-γ or IFN-γ + LPS is discussed.

10.1177/09680519040100010401

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Citations (15)

[A case of hepatocellular carcinoma with solitary lymph node metastasis after liver resection].

PubMed (1997)

Yoshifumi Fujimori Shoji Kajikawa S Nakata Nobuhiko Shimozawa H Watanabe

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Citations (0)

A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics (2016)

Yoshiro Kato Yoshikazu Naiki Takayuki Komatsu Kazuko Takahashi Jiro Nakamura

A Wnt agonist, 2-amino-4-[3,4-(methylenedioxy)benzylamino]-6-(3-methoxyphenyl) pyrimidine, is a cell-permeable pyrimidine compound that has been shown to mimic the effect of Wnt. In this study, leukemic mouse cell lines, RAW 264.7 and J774.1, were incubated with the Wnt agonist. The Wnt agonist showed cell death in the concentration of 1‐10 μM. The Wnt agonist did not show inhibition of GSK-3β activity but induced β-catenin accumulation in the nucleus. The Wnt agonist showed caspase-independent cell death, but no further involvement in cell death ER stress signaling. Here we discuss the possible mechanism of Wnt agonist-induced apoptotic cell death in RAW 264.7 cells.

10.3727/096504016x14721731148893

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Citations (3)

1P-0251 Apoptosis of smooth muscle cells induced by oxysterol was enhanced under diabetic condition

Atherosclerosis Supplements (2003)

Yasushi Miyashita Ken-ichi Endoh Aya Saiki Hiroshi Ozaki M Ohtsuka

Oxysterol

10.1016/s1567-5688(03)90322-9

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Citations (1)

Combined semi-nested polymerase chain reaction and heteroduplex analysis for detecting monoclonality of IgH rearrangement in patients with follicular lymphoma.

PubMed (2004)

Amaylia Oehadian Naoki Koide Mya Mya Mu Ferdaus Hassan Tomoaki Yoshida

A new, sensitive method combining seminested polymerase chain reaction (PCR) and heteroduplex analysis was used to detect follicular lymphoma (FL) cells in peripheral blood. Based on the detection of IgH rearrangement in DNA from peripheral blood leukocytes, the method demonstrated the presence of monoclonal B cells in FL patients with high frequency.

Heteroduplex

Follicular lymphoma

Gene rearrangement

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Citations (0)

The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS

Journal of Endotoxin Research (2001)

Tsuyoshi Sugiyama Naoki Koide Dipshikha Chakravortty Yutaka Kato Mya Mya Mu

The surface expression of CD14 on mouse B-1 cells and its role on their response to lipopolysaccharide (LPS) were studied by using the murine TH2.52 B-1 cell line and peritoneal B-1 cells. TH2.52 cells with the B-1 phenotype were found to express membrane-bound CD14. Furthermore, CD14 was expressed on physiological peritoneal CD5 + B-1 cells. The stimulation of CD14-expressing TH2.52 cells with a low concentration of LPS resulted in the activation of nuclear factor (NF)-κB and a mitogen-activated protein kinase (MAPK). The LPS-induced NF-κB and MAPK activation was markedly inhibited by anti-CD14 antibody. These results suggest that B-1 cells may respond to LPS via membrane-bound CD14.

10.1177/09680519010070030501

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Citations (5)

Cell-surface immunoglobulin and insulin receptor expression in an EBV-negative lymphoma cell line and its EBV-converted sublines.

The Journal of Immunology (1981)

G. Spira Pierre Åman Naoki Koide Gunnel Lundin Georg Klein

Membrane Ig and insulin receptors were assayed in the EBV-negative Ramos lymphoma line and its EBV-converted sublines by surface fluorescence. 125I-protein A-binding assay, immunoprecipitation, and insulin receptor assay. The original Ramos line expressed surface IgM but not IgD and had a low concentration of insulin receptors. Ten of 10 converted lines expressed IgD and a variable but usually high insulin-binding capacity. Molecular weight analysis of immunoprecipitated Ig showed the presence of 2 mu-chains and 2 delta-chains. One of each heavy chain type could be characterized as membrane Ig, whereas the other was cytoplasmic. Antisera against the lambda-chain precipitated mu and delta heavy chains as well, indicating that the delta-chain expressed on the membrane of the EBV-converted Ramos lines is bound to the lambda light chain. The results show that the differentiation of a Burkitt lymphoma line is not completely "frozen" and can be induced to change in a more "activated" direction by EBV conversion.

Immunoglobulin D

Immunoprecipitation

Surface Immunoglobulin

Cell surface receptor

Immunoglobulin heavy chain

10.4049/jimmunol.126.1.122

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Citations (38)