Numan Karaarslan

Haliç University

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i-10 index

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Co-Authors

İY

İbrahim Yılmaz

Mayo Clinic in Florida

HÖ

Hanefi Özbek

Bakırçay Üniversitesi

Duygu Yaşar Şirin

Namık Kemal University

ÖA

Özkan Ateş

Koç University

TÇ

Tezcan Çalışkan

Namık Kemal University

Necati Kaplan

TC İstanbul Rumeli Üniversitesi

Yasin Emre Kaya

Bolu Abant İzzet Baysal University

Abdullah Talha Şimşek

Istanbul Medeniyet University

Mustafa Doğan

Namık Kemal University

Hande Akalan

Namık Kemal University

Cooperative Institutions

Namık Kemal University

Istanbul Medeniyet University

Sağlık Bilimleri Üniversitesi

Istanbul Medipol University

Ministry of Health

Haydarpaşa Numune Eğitim ve Araştırma Hastanesi

Istanbul University

Marmara University

Istanbul Eye Hospital

University of Health Science

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Characteristics of Acromegaly Patients Followed at a Single Center

Turkish Journal of Endocrinology and Metabolism (2018)

Gülşah Elbüken Orhan YAĞMUR İsmail Yıldız Tamer Tunçkale Tezcan Çalışkan

Center (category theory)

10.25179/tjem.20182202-p066

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Citations (0)

The association between different molecular weights of hyaluronic acid and CHAD, HIF-1α, COL2A1 expression in chondrocyte cultures

Experimental and Therapeutic Medicine (2018)

Duygu Si̇rin Necati Kaplan İbrahim Yılmaz Numan Karaarslan Hanefi Özbek

The aim of the present study was to investigate the effects of three different formulations of hyaluronic acid (HA): Low molecular weight (MW) Sinovial One®, medium MW Viscoplus® and high MW Durolane®, on chondrocyte proliferation and collagen type II (COL2A1), hypoxia-inducible factor 1α (HIF-1α) and chondroadherin (CHAD) expression in primary chondrocyte cultures. Standard primary chondrocyte cultures were established from osteochondral tissues surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted light microscope; cell proliferation was determined with a MTT assay and confirmed with acridine orange/propidium iodide staining. Levels of CHAD, COL2A1 and HIF-1α expression were assessed using specific TaqMan gene expression assays. The results demonstrated the positive effect of HA treatment on cell proliferation, which was independent from the MW. COL2A1 expression increased in the medium and high MW HA treated groups. It was observed that HIF-1α expression increased in the high MW treated group alone. CHAD expression increased only in the medium MW HA treated group. Evaluation of gene expression revealed that levels of expression increased as the duration of HA application increased, in the medium and high MW HA treated groups. In terms of increased viability and proliferation, a longer duration of HA application was more effective. Taken together, it may be concluded that the administration of medium and high MW HA may be a successful way of treating diseases affecting chondrocytes in a clinical setting.

Acridine orange

Propidium iodide

Viability assay

MTT assay

10.3892/etm.2018.5943

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Citations (15)

CT-guided Stereotactic Microsurgical Resection of Cerebral Mass Lesions

Medeniyet Medical Journal (2018)

Mehmet Erşahin Mehmet Sabri Gürbüz Numan Karaarslan Ahmet Ferruh Gezen

10.5222/mmj.2018.87259

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Citations (0)

Are specific gene expressions of extracellular matrix and nucleus pulposus affected by primer cell cultures prepared from intact or degenerative intervertebral disc tissues?

Turkish Neurosurgery (2018)

Numan Karaarslan İbrahim Yılmaz Hanefi Özbek Duygu Yaşar Şirin Necati Kaplan

To determine the gene expression patterns of nucleus pulposus (NP) in cell cultures obtained from degenerated or intact tissues.Whereas 12 of the cases were diagnosed with lumbar disc herniation and had undergone lumbar microdiscectomy, 12 cases had undergone traumatic intervertebral discectomy and corpectomy, along with discectomy after spinal trauma. NP-specific markers and gene expressions of the reagents of the extracellular matrix in the experimental setup were tested at the 0th, 24th, and 48th hours by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Visual evaluations were simultaneously made in all samples using invert and fluorescence microscopy. Vitality and proliferation analyses were evaluated by UV spectrophotometer. As a method of statistical evaluation, Spearman was used for categorical variants, and the Pearson correlation was used for variants with numerical and plain distribution.No association was found either between the tissue type and times (r=0.000; p=1.000) or between the region that the tissue was obtained from and hypoxia transcription factor-1 alpha (HIF-1α) gene expression (r=0.098; p=0.245). There was no correlation between cell proliferation and chondroadherin (CHAD) expression or between type II collagen (COL2A1) and CHAD gene expressions. It was found that CHAD and HIF-1α gene expressions and HIF-1α and COL2A1 gene expressions affected cell proliferation.Cell culture setups are of paramount importance because they may influence the pattern of changes in the gene expressions of the cells used in these setups.

Intervertebral disk

Discectomy

MMP1

10.5137/1019-5149.jtn.22210-17.2

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Citations (7)

Pharmaco-molecular assessment of the effects of anandamide and its antagonists on hippocampal tissue in Wistar albino rats

DOAJ (DOAJ: Directory of Open Access Journals) (2020)

İbrahim Yılmaz Numan Karaarslan Duygu Yaşar Şirin Hanefi Özbek

OBJECTIVE The members of the matrix metalloproteinase (MMP) family and cannabinoids (CBs) are reportedly associated with hippocampus-dependent memory functions. However, the effects of endogenously formed CBs on hippocampal long-term potentiation remain unknown. The present study aimed to investigate the changes in the gene and protein expression levels of matrix metallopeptidase 9 (MMP-9), phosphatase and tensin homolog (PTEN), and NOTCH receptor 1 (NOTCH1) in rat hippocampal tissues treated with anandamide (AEA), AM251, 6-iodopravadolin (AM630), and N-[4-{[(3,4-Dimethyl-5-isoxazolyl)amino]sulfonyl}phenyl] (ML193). MATERIALS AND METHODS The subjects were divided into 10 groups (n = five per group). The pharmaceuticals were administered via intraperitoneal injection once a day for seven days, except for the control group. The resected hippocampal tissues were then evaluated using a quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot analysis. The data obtained were statistically analyzed, and p < 0.01 was considered statistically significant. RESULTS Contrary to the literature, the changes in MMP-9 expression were not statistically significant, but the changes in PTEN and NOTCH1 were. The findings of this in vivo experimental study revealed that the agonists and antagonists acting on the CB system have significant molecular effects on hippocampal tissue. CONCLUSIONS The changes in gene and protein expressions may be one of the reasons for the neurodegenerative processes observed in patients using these agonists and antagonists, whose effects on the CB system have not been fully explained yet. Our study can contribute to the literature as it is the first study investigating the MMP-9, PTEN and NOTCH1 gene and protein expression.

Tensin

Anandamide

AM251

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Citations (4)

Remote Cerebellar Haemorrhage after Burr Hole Drainage of Chronic Subdural Haematoma: A Case Report

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH (2016)

Mehmet Sabri Gürbüz Numan Karaarslan Şevki Gök Celaleddin Soyalp

Remote cerebellar haemorrhage (RCH) is an unusual complication of supratentorial neurosurgical procedures. Even the rarer is cerebellar haemorrhage occurring after supratentorial burr hole drainage of Chronic Subdural Haematoma (CSDH). The exact mechanism is still unclear despite some possible causative factors such as rapid evacuation of haematoma and overdrainage of CSF (Cerebrospinal Fluid). We report a 80-year-old male patient who developed cerebellar haemorrhage after burr hole drainage of left frontoparietal chronic subdural haematoma and discuss the possible aetiological mechanisms through the review of the current literature.

Etiology

Chronic Subdural Hematoma

10.7860/jcdr/2016/17218.7710

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Citations (6)

A study of the effects of metformin, a biguanide derivative, on annulus fibrosus and nucleus pulposus cells

Turkish Neurosurgery (2019)

Yasin Emre Kaya Numan Karaarslan İbrahim Yılmaz Duygu Yaşar Şirin Hande Akalan

To investigate the effects of metformin, a drug used widely for the treatment of type 2 diabetes mellitus, on human primary cell cultures prepared from uninjured segment of disc material intervertebral disk tissues.Primary cell cultures were prepared using the tissues of six patients (three males and three females) who had undergone lumbar microdiscectomy and sequestrectomy. Untreated samples served as the control group, and metformintreated samples served as the experimental group. All the samples were evaluated using an inverted light microscope, acridine orange/propidium iodide staining (AO/PI), and a fluorescence microscope. The cytostatic and cytotoxic effects of metformin, which was administered to the samples using a commercial MTT assay kit, were also evaluated. The data obtained were statistically assessed, and the alpha significance value was accepted as less than 0.05. In addition, for the groupsâ€™ changes in the expressions of chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), interleukin-1Î² (IL-1Î²) matrix metalloproteinase 7 (MMP-7), and matrix metalloproteinase 19 (MMP-19), genes related to the extracellular matrix synthesis and degradation were determined using gene-specific TaqMan Gene Expression Assays.The administration of the drug adversely affected nucleus pulposus (NP)/annulus fibrosus (AF) cells and extracellular matrixâ€"like structures. This was statistically significant (p < 0.05).Clinicians should not disregard the adverse effects of metformin, which is used widely in clinical practice, on the components of intervertebral disk tissues.

Intervertebral Disc

Acridine orange

MTT assay

Propidium iodide

10.5137/1019-5149.jtn.27785-19.3

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Citations (5)

Investigation of the potential effects of alpha-lipoic acid on human degenerated intervertebral disc tissue primary cell cultures

Turkish Neurosurgery (2021)

İrfan Aydın Duygu Yaşar Şirin Numan Karaarslan Hanefi Özbek

To investigate the supplementation of alpha-lipoic acid (ALA) at the molecular level to determine its effect on primary cell cultures prepared from human intervertebral disc (IVD) tissue in an in vitro environment.Human primary cell cultures were prepared from IVD tissue resected during surgery. While cell cultures without ALA supplementation formed the control group, those with ALA supplementation formed the study group. All cell groups were stained using acridine orange/propidium iodide (AO/PI), and the incidence of apoptotic cell death was determined under a fluorescent microscope. Cell surface morphology and extracellular matrix (ECM) structures were evaluated under an invert light microscope. Simultaneously, cell proliferation was evaluated by MTT?ELISA analysis, and the expressions of chondroadherin (CHAD), cartilage oligomeric protein (COMP), interleukin-1 beta (IL-1?), and matrix metalloproteinase (MMP)-7 and-19, which are genes associated with ECM regulation, were tested using qRT?PCR. The data obtained were evaluated statistically using Tukey?s honestly significant difference (HSD) test after analysis of variance (ANOVA) was performed. The alpha significance value was accepted as < .05.Compared to the cells in the control group, it was observed that both proliferation was suppressed and ECM structures deteriorated in the cells in the study group.Also, it was reported that the all-gene expression levels changed. ALA supplementation can negatively affect human IVD primary cell cultures in an in vitro environment.

Acridine orange

Propidium iodide

MTT assay

Intervertebral Disc

10.5137/1019-5149.jtn.34917-21.3

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Citations (2)

The effect of matrix metalloproteinase enzyme-3 on the prognosis and biological behaviour of meningiomas

Turkish Neurosurgery (2015)

Numan Karaarslan Mehmet Sabri Gürbüz Tezcan Çalışkan Erdoğan Ayan Fügen Aker

To analyse the effect of MMP-3 (Matrix Metalloproteinase Enzyme-3)-one of the extracellular matrix proteins- on the prognosis and biological behaviour of meningiomas.79 cases of meningioma that were operated in our clinic between 2005 and 2010 were retrospectively analysed. Age, sex, preoperative peritumoral edema, histological subtype, grade, Ki-67 expression, MMP-3 staining pattern and recurrence rate were analysed. Pathological preparations were graded according to the WHO (World Health Organisation) 2007 grading system.Of the MMP-III positive cases; 24 cases (60%) were grade I, 16 cases were grade II. The MMP-3 staining pattern was significantly positive (80%) in grade II meningioma. 14 of the MMP-3 positive cases were atypical meningiomas. Of the 20 cases with high Ki-67 proliferation index (PI), 12 cases (60%) were MMP-3 positive and 8 cases (40%) were MMP-3 negative. Rates of recurrence and preoperative peritumoral edema were high in cases with MMP-3 positivity.In this study it was determined that MMP-3 positivity has a strong relationship with meningiomas having an aggressive character. MMP-3 may be used as a proliferation marker for biological behaviour, recurrence rate and prognosis of meningiomas.

Grading (engineering)

Matrix metalloproteinase 9

Matrix Metalloproteinase 3

Ki-67

Proliferation index

10.5137/1019-5149.jtn.12807-14.1

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Citations (2)

Remote Cerebellar Haemorrhage Occurring after Transcranial Pituitary Surgery

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH (2017)

Numan Karaarslan Mehmet Sabri Gürbüz Tezcan Çalışkan Tamer Tunçkale

A 63-year-old male patient was referred to the Department of Neurosurgery with a six-month history of gradually increasing headache and a three-month history of visual impairment.The physical and laboratory examinations were unremarkable.No neurological deficit was detected other than bitemporal hemianopsia.Cranial Magnetic Resonance Imaging (MRI) scan revealed a pituitary macroadenoma, expanding suprasellar cistern and compressing the optic chiasm [Table /Fig-1].Preoperative coagulation parameters were within normal limits.After preoperative evaluations the patient underwent an operation of gross total excision of pituitary macroadenoma via a right pterional craniotomy.Upon deterioration of consciousness with a Glasgow Coma Score (GCS) of 10 and emerging of a hypertensive attack on the third postoperative day, a non-contrast enhanced cranial CT (Computed Tomography) scan was taken.A left cerebellar haematoma causing partial compression of the fourth ventricle and mild hydrocephalus were detected b].An external ventricular drain was immediately placed in order to decrease and control the intracranial pressure.No further surgical intervention was considered and the patient was followed in intensive care unit.On the first postoperative day of external ventricular drain insertion, the patient's neurological status improved to normal levels (GCS: 15) and no neurological deficit was observed.On the seventh day of cerebellar haemorrhage, cranial CT scan revealed the nearly-total resolution of cerebellar haemorrhage .The patient's drain was pulled out on

Etiology

10.7860/jcdr/2017/29152.10465

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Citations (2)