Alessa Schipperges

Karlsruhe Institute of Technology

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Christof M. Niemeyer

Karlsruhe Institute of Technology

Yong Hu

Tongji University

Kersten S. Rabe

Karlsruhe Institute of Technology

Simone Weigel

Karlsruhe Institute of Technology

Christof Wöll

Karlsruhe Institute of Technology

Carmen M. Domínguez

Karlsruhe Institute of Technology

J. Bauer

Maastricht University

Claude Oelschlaeger

Karlsruhe Institute of Technology

Diana Ordoñez‐Rueda

European Molecular Biology Laboratory

Stephan Keppler

Karlsruhe Institute of Technology

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Karlsruhe Institute of Technology

Karlsruhe University of Education

Interface (United States)

FIZ Karlsruhe – Leibniz Institute for Information Infrastructure

University of Duisburg-Essen

Technical University of Munich

Karlsruhe University of Applied Sciences

Mechanics' Institute

University of Fribourg

TU Dortmund University

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Multiscale Origami Structures as Interfaces for Cells (MOSAIC) – A versatile platform for the investigation of early cell signalling

Klavdiya Gordiyenko Alessa Schipperges Christof M. Niemeyer

Mosaic

Signalling

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Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings

Nature Communications (2019)

Yong Hu Carmen M. Domínguez J. Bauer Simone Weigel Alessa Schipperges

Abstract Biomedical applications require substrata that allow for the grafting, colonization and control of eukaryotic cells. Currently available materials are often limited by insufficient possibilities for the integration of biological functions and means for tuning the mechanical properties. We report on tailorable nanocomposite materials in which silica nanoparticles are interwoven with carbon nanotubes by DNA polymerization. The modular, well controllable and scalable synthesis yields materials whose composition can be gradually adjusted to produce synergistic, non-linear mechanical stiffness and viscosity properties. The materials were exploited as substrata that outperform conventional culture surfaces in the ability to control cellular adhesion, proliferation and transmigration through the hydrogel matrix. The composite materials also enable the construction of layered cell architectures, the expansion of embryonic stem cells by simplified cultivation methods and the on-demand release of uniformly sized stem cell spheroids.

10.1038/s41467-019-13381-1

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Formulation of DNA Nanocomposites: Towards Functional Materials for Protein Expression

Polymers (2021)

Alessa Schipperges Yong Hu Svenja Moench Simone Weigel Johannes Reith

DNA hydrogels are an emerging class of materials that hold great promise for numerous biotechnological applications, ranging from tissue engineering to targeted drug delivery and cell-free protein synthesis (CFPS). In addition to the molecular programmability of DNA that can be used to instruct biological systems, the formulation of DNA materials, e.g., as bulk hydrogels or microgels, is also relevant for specific applications. To advance the state of knowledge in this research area, the present work explores the scope of a recently developed class of complex DNA nanocomposites, synthesized by RCA polymerization of DNA-functionalized silica nanoparticles (SiNPs) and carbon nanotubes (CNTs). SiNP/CNT–DNA composites were produced as bulk materials and microgels which contained a plasmid with transcribable genetic information for a fluorescent marker protein. Using confocal microscopy and flow cytometry, we found that the materials are very efficiently taken up by various eukaryotic cell lines, which were able to continue dividing while the ingested material was evenly distributed to the daughter cells. However, no expression of the encoded protein occurred within the cells. While the microgels did not induce production of the marker protein even in a CFPS procedure with eukaryotic cell lysate, the bulk composites proved to be efficient templates for CFPS. This work contributes to the understanding of the molecular interactions between DNA composites and the functional cellular machinery. Implications for the use of such materials for CFPS procedures are discussed.

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10.3390/polym13152395

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