Sanjay Bharati

Manipal Academy of Higher Education

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Sachin Shetty

Manipal Academy of Higher Education

Ashwani Koul

University of Jammu

Rajesh Kumar

All India Institute of Medical Sciences Jodhpur

Praveen Rishi

Panjab University

U Anushree

Manipal Academy of Higher Education

Prasad Kisan Tambe

Manipal Academy of Higher Education

Abhinav Kanwal

All India Institute of Medical Sciences

Jesil Mathew Aranjani

Manipal Academy of Higher Education

H.S. Qsee

Manipal Academy of Higher Education

Navjot Kanwar

Maharaja Ranjit Singh Punjab Technical University

Cooperative Institutions

Manipal Academy of Higher Education

Panjab University

Kasturba Medical College, Manipal

All India Institute of Medical Sciences

University of the Punjab

University of Trieste

Melaka Manipal Medical College

Engineering (Italy)

All India Institute of Medical Sciences Jodhpur

Kasturba Medical College, Mangalore

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

MitoQ demonstrates connexin- and p53-mediated cancer chemoprevention in N-nitrosodiethylamine-induced hepatocarcinogenesis rodent model

Toxicology and Applied Pharmacology (2022)

H.S. Qsee Prasad Kisan Tambe Shounak De Sanjay Bharati

10.1016/j.taap.2022.116211

Cite

Citations (7)

Selenium attenuates venlafaxine hydrochloride‐induced testicular damage in mice via modulating oxidative stress and apoptosis

Andrologia (2021)

Sarvnarinder Kaur Amarjit Kaur Nisha Jaswal Aniqa Aniqa Shilpa Sadwal

The present study assessed the effect of selenium (Se) supplementation on Venlafaxine hydrochloride (VH)-induced testicular toxicity. Mice were segregated into Group I (C), Group II (0.5 ppm Se), Group III (VH at a dose 60 mg/kg b.w.) and Group IV (Se was given as per Group II, and VH was given as per Group III). After 10 weeks, sperm parameters, histology, sperm cell counts, antioxidants activities, apoptotic proteins and molecular analysis of testicular tissue were evaluated. Group III had significantly lower sperm concentration (from 2.17 ± 0.28 to 1.04 ± 0.22) and sperm motility (from 68.04 ± 5.5 to 21.47 ± 5.21), and showed an extensive vacuolisation in the germinal epithelium, abnormal basement membrane, and reduced germ cell number as compared to Group I. However, selenium supplementation in Group IV substantially increased sperm concentration (1.47 ± 0.48) and motility (33.27 ± 8.66), improved the histoarchitecture and repopulated the germ cells as observed by raised numbers of spermatogonia, spermatocytes, round spermatids and elongated spermatids contrasted to Group III. Group IV also showed a noteworthy decreased ROS, LPO levels, as well as expressions of Bax, caspase-9, and caspase-3 and increased the SOD, CAT, GPx, and GSH activities as well the expression of Bcl-2 as compared to Group III. This effect was further supported by FTIR analysis for nucleic acids. Thus, selenium supplementation showed significant protection against VH-induced testicular toxicity.

Germinal epithelium

10.1111/and.14050

Cite

Citations (7)

List of contributors

Elsevier eBooks (2021)

Suzana Aulić Sanjay Bharati Sri Pragnya Cheruku Z. Ellederova Maurizio Fermeglia

10.1016/b978-0-323-85050-6.00019-0

Cite

Citations (0)

COCERIG ISSUES WITH BIOSIMILARS: AOVERVIEW

Usha Rani Swarna Sahith Reddy Sanjay Bharati

Summary Biopharmaceuticals are biological medicinal products that have been developed through biotechnological practices, including recombinant human technology, gene technology or antibody methods. The imminent patent expiration of many biopharmaceutical products will produce the possibility for generic versions of these therapeutic agents (i.e.biosimilars). Biosimilars differ from generic low molecular weight chemical drugs in many important ways. These include the size and complexity of the active substance, which will affect the scientific requirement for testing; the nature of the starting materials (cell banks, tissues, and other biological products); the complexity of the manufacturing processes; and the limitations of state-ofthe-art methods to characterize proteins and to detect all product variations that can influence clinical efficacy, sideeffects like immunogenicity. Therefore, it was acknowledged that established legal and regulatory principles of ‘essential similarity’ that are applied to standard generics cannot be readily applied to biosimilars. Thus, verification of the similarity to or substitutability of biosimilars with reference innovator biopharmaceutical products will require much more than a demonstration of pharmacokinetic similarity, which is sufficient for

Biosimilar

Biopharmaceutical

Innovator

Interchangeability

Biological drugs

Source

Cite

Citations (0)

Peptides as adjuvants for ampicillin and oxacillin against methicillin-resistant Staphylococcus aureus (MRSA)

Microbial Pathogenesis (2018)

Praveen Rishi Shania Vij Indresh Kumar Maurya Ujjwal Jit Kaur Sanjay Bharati

Efflux

Ethidium bromide

10.1016/j.micpath.2018.08.023

Cite

Citations (22)

Cardioprotective potential of mitochondria-targeted antioxidant, mito-TEMPO, in 5-fluorouracil-induced cardiotoxicity

Cancer Chemotherapy and Pharmacology (2023)

Prasad Kisan Tambe Jesil Mathew Aranjani Sanjay Bharati

The mitochondria-targeted antioxidants (MTAs) are known to offer protection against mitochondrial oxidative stress. The recent evidences support their role in mitigating oxidative stress-induced diseases, including cancer. Therefore, this study investigated cardioprotective potential of mito-TEMPO against 5-FU-induced cardiotoxicity.Mito-TEMPO was administered to male BALB/C mice (intraperitoneally, 0.1 mg/kg b.w. for 7 days) followed by intraperitoneal administration of 5- FU (12 mg/kg b.w. for 4 days). During this period, mito-TEMPO treatment was also continued. The cardioprotective potential of mito-TEMPO was assessed by evaluating cardiac injury markers, extent of non-viable myocardium and histopathological alterations. Mitochondrial functional status and mitochondrial oxidative stress were assessed in cardiac tissue. 8-OHdG expression and apoptotic cell death were assessed using immunohistochemical techniques.The level of cardiac injury markers CK-MB and AST were significantly (P ≤ 0.05) decreased in mito-TEMPO pre-protected group which was further reflected in histopathology as decrease in the percentage of non-viable myocardial tissue, disorganization, and loss of myofibrils. Mito-TEMPO ameliorated mtROS, mtLPO and conserved mitochondrial membrane potential. Further, it had significantly (P ≤ 0.05) improved the activity of mitochondrial complexes and mitochondrial enzymes. A significant (P ≤ 0.05) increase in the level of mtGSH, activity of mitochondrial glutathione reductase, glutathione peroxidase, and mitochondrial superoxide dismutase was observed. A decreased expression of 8-OHdG and reduced apoptotic cell death were observed in mito-TEMPO pre-protected group.Mito-TEMPO effectively mitigated 5-FU-induced cardiotoxicity by modulating mitochondrial oxidative stress, hence may serve as a protective agent/adjuvant in 5-FU-based combinatorial chemotherapy.

Cardiotoxicity

Glutathione reductase

10.1007/s00280-023-04529-4

Cite

Citations (5)

Exploring New Drug Targets for Type 2 Diabetes: Success, Challenges and Opportunities

Biomedicines (2022)

Abhinav Kanwal Navjot Kanwar Sanjay Bharati Prateek Srivastava Shailendra Pratap Singh

There are substantial shortcomings in the drugs currently available for treatment of type 2 diabetes mellitus. The global diabetic crisis has not abated despite the introduction of new types of drugs and targets. Persistent unaddressed patient needs remain a significant factor in the quest for new leads in routine studies. Drug discovery methods in this area have followed developments in the market, contributing to a recent rise in the number of molecules. Nevertheless, troubling developments and fresh challenges are still evident. Recently, metformin, the most widely used first-line drug for diabetes, was found to contain a carcinogenic contaminant known as N-nitroso dimethylamine (NDMA). Therefore, purity and toxicity are also a big challenge for drug discovery and development. Moreover, newer drug classes against SGLT-2 illustrate both progress and difficulties. The same was true previously in the case of glucagon-like peptide-1 receptor agonists and dipeptidyl peptidase-4 inhibitors. Furthermore, researchers must study the importance of mechanistic characteristics of novel compounds, as well as exposure-related hazardous aspects of current and newly identified protein targets, in order to identify new pharmacological molecules with improved selectivity and specificity.

10.3390/biomedicines10020331

Cite

Citations (38)

Quality assessment of Tc-99m methylene diphosphonate (MDP) radiopharmaceutical prepared using different cold kit fractionation methods

Indian Journal of Nuclear Medicine (2022)

Arun Kumar Thokchom Rajesh Kumar Sanjay Bharati Vasumathi

Purpose: Tc-99m Methylene diphosphonate (MDP) is prepared in house by labeling MDP cold kit. Each kit is a single time use vial and contains large amounts of reagent sufficient for preparing multiple doses. Therefore, several centers are adopting the practice of fractionating the MDP kit so that it can be used multiple times. The aim of the study was to evaluate the effect of kit fractionation on radiopharmaceutical property. Materials and Methods: The MDP kit was fractionated using two different approaches, namely, vial and syringe method. The quality of Tc-99m MDP prepared using these approaches was assessed and compared with that prepared by the conventional method. The image quality was evaluated in a total of 100 patients. Results: The vial and syringe fractionated Tc-99m MDP showed >95% RCP till the 4 th and 2 nd days of fractionation, respectively. Percentage radiochemical purity deteriorated to 83.6% and 88% on the 8 th day of fractionation in the vial and syringe method, respectively. No microbial growth was observed in any of these methods till the 8 th day of fractionation. The reconstituted MDP solution during all preparations was clear and colorless in appearance with pH ranging from 6.5 to 7.5. The image contrast, contrast-to-noise, and signal-to-noise ratio were statistically similar in both methods compared to the conventional method until the 2 nd day of fractionation. The image quality data showed no statistical difference among images of vial and syringe fractionated MDP as compared to the conventional unfractionated Tc-99m MDP. Conclusions: The observations revealed that if fractionated with utmost care, both methods yield almost similar results.

Vial

10.4103/ijnm.ijnm_115_21

Cite

Citations (5)

Chemopreventive potential of hydroethanolic Murraya koenigii leaves extract against DMBA induced breast carcinogenesis: In-silico and in-vivo study

Journal of Ethnopharmacology (2023)

Shilpa Sadwal Sanjay Bharati Zahid Ahmad Dar Sarvnarinder Kaur

Murraya

Phytochemical

10.1016/j.jep.2023.117124

Cite

Citations (2)

Convolutional neural network for colorimetric glucose detection using a smartphone and novel multilayer polyvinyl film microfluidic device

Scientific Reports (2024)

Mithun Kanchan Prasad Kisan Tambe Sanjay Bharati Omkar S Powar

Abstract Detecting glucose levels is crucial for diabetes patients as it enables timely and effective management, preventing complications and promoting overall health. In this endeavor, we have designed a novel, affordable point-of-care diagnostic device utilizing microfluidic principles, a smartphone camera, and established laboratory colorimetric methods for accurate glucose estimation. Our proposed microfluidic device comprises layers of adhesive poly-vinyl films stacked on a poly methyl methacrylate (PMMA) base sheet, with micro-channel contours precision-cut using a cutting printer. Employing the gold standard glucose-oxidase/peroxidase reaction on this microfluidic platform, we achieve enzymatic glucose determination. The resulting colored complex, formed by phenol and 4-aminoantipyrine in the presence of hydrogen peroxide generated during glucose oxidation, is captured at various glucose concentrations using a smartphone camera. Raw images are processed and utilized as input data for a 2-D convolutional neural network (CNN) deep learning classifier, demonstrating an impressive 95% overall accuracy against new images. The glucose predictions done by CNN are compared with ISO 15197:2013/2015 gold standard norms. Furthermore, the classifier exhibits outstanding precision, recall, and F1 score of 94%, 93%, and 93%, respectively, as validated through our study, showcasing its exceptional predictive capability. Next, a user-friendly smartphone application named “GLUCOLENS AI” was developed to capture images, perform image processing, and communicate with cloud server containing the CNN classifier. The developed CNN model can be successfully used as a pre-trained model for future glucose concentration predictions.

Glucose oxidase

10.1038/s41598-024-79581-y

Cite

Citations (0)