Mingxing Tian

Shanghai Veterinary Research Institute

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Shengqing Yu

Chinese Academy of Agricultural Sciences

Chan Ding

Shanghai Veterinary Research Institute

Shaohui Wang

Shanghai Veterinary Research Institute

Jingjing Qi

Shanghai Veterinary Research Institute

Tao Li

Chinese Academy of Agricultural Sciences

Yanqing Bao

Shanghai Veterinary Research Institute

Xiangan Han

Shanghai Veterinary Research Institute

Yi Yin

Nanjing Drum Tower Hospital

Hai Hu

Hangzhou Cancer Hospital

Xiaolan Wang

Guangdong Academy of Medical Sciences

Cooperative Institutions

Chinese Academy of Agricultural Sciences

105

Shanghai Veterinary Research Institute

Chinese Academy of Sciences

Shanghai Jiao Tong University

Ministry of Agriculture and Rural Affairs

Nanjing University

Shandong University

Yangzhou University

Binzhou Medical University

Sun Yat-sen University

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Mechanistic Insights into Stereospecific Bioactivity and Dissipation of Chiral Fungicide Triticonazole in Agricultural Management

Journal of Agricultural and Food Chemistry (2018)

Qing Zhang Zhaoxian Zhang Bowen Tang Beibei Gao Mingxing Tian

Research interest in chiral pesticides has increased probably because enantiomers often exhibit different environmental fate and toxicity. An investigation into the enantiomer-specific bioactivity of chiral triticonazole enantiomers in agricultural systems revealed intriguing experimental and theoretical evidence. For nine of the phytopathogens studied ( Rhizoctonia solani, Fusarium verticillioide, Botrytis cinerea (strawberry and tomato), Rhizoctonia cereali, Alternaria solani, Gibberella zeae, Sclerotinia sclerotiorum, and Pyricularia grisea), the fungicidal activity data showed ( R)-triticonazole was 3.11-82.89 times more potent than the ( S) enantiomer. Furthermore, ( R)-triticonazole inhibited ergosterol biosynthesis and cell membrane synthesis 1.80-7.34 times higher than its antipode. Homology modeling and molecular docking studies suggested the distinct bioactivities of the enantiomers of triticonazole were probably due to their different binding modes and affinities to CYP51b. However, field studies demonstrated that ( S)-triticonazole was more persistent than ( R)-triticonazole in fruits and vegetables. The results showed that application of pure ( R)-triticonazole, with its high bioactivity and relatively low resistance risk, instead of the racemate in agricultural management would reduce the application dosage required to eliminate carcinogenic mycotoxins and any environmental risks associated with this fungicide, yielding benefits in food safety and environmental protection.

Alternaria solani

10.1021/acs.jafc.8b01771

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Citations (50)

Characteristics of Brucella abortus vaccine strain A19 reveals its potential mechanism of attenuated virulence

Veterinary Microbiology (2021)

Zhimin Cheng Zichen Li Yi Yin Zhengmin Lian Hosny Ahmed Abdelgawad

Attenuated vaccine

Strain (injury)

10.1016/j.vetmic.2021.109007

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Citations (11)

The putative amino acid ABC transporter substrate-binding protein AapJ2 is necessary for Brucella virulence at the early stage of infection in a mouse model

Veterinary Research (2018)

Mingxing Tian Yanqing Bao Peng Li Hai Hu Chan Ding

Brucellosis is a zoonotic bacterial disease caused by Brucella spp. The virulence of these bacteria is dependent on their ability to invade and replicate within host cells. In a previous study, a putative gene bab_RS27735 encoding an amino acid ABC transporter substrate-binding protein homologous to AapJ protein was found to be involved in Brucella abortus virulence. In this study, we successfully constructed a bab_RS27735 deletion mutant, Δ27735. Compared with the wild-type strain, the lipopolysaccharide pattern of the mutant was not changed, but the growth ability was slightly defected in the exponential phase. In tolerance tests, sensitivity of the Δ27735 mutant to oxidative stress, bactericidal peptides or low pH was not different from that of the wild-type strain. Cell infection assay showed that the mutant was reduced survival within macrophages but could efficiently escape lysosome degradation. The results of a virulence test showed that the Δ27735 mutant was attenuated in a mouse model at the early stage of infection but recovered its virulence at the late stage of infection. Meanwhile, the development of splenomegaly and histopathological lesions was observed in mice infected with either the wild-type strain or the mutant. These results are in line with the release of IL-12p40 and TNF-α into the peripheral blood of infected mice. Besides, expression of diverse genes was up-regulated in the Δ27735 mutant, which may contribute to the reduced virulence of the mutant. These data elucidated that the bab_RS27735 gene is necessary for B. abortus virulence at the early stage of infection in a mouse model.

Brucellaceae

Wild type

Attenuated vaccine

10.1186/s13567-018-0527-9

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Citations (9)

DctR contributes to the virulence of avian pathogenic Escherichia coli through regulation of type III secretion system 2 expression

Veterinary Research (2021)

Yaodong Zhang Yao Wang Hong Zhu Zhengfei Yi Dossêh Jean Apôtre Afayibo

Pathogens could precisely alter their gene expression to facilitate their survival and successful infection. The LuxR family transcriptional regulator DctR (also known as YhiF) was shown to participate in the regulation of acid fitness and adhesion of enterohemorrhagic E. coli (EHEC) O157:H7. Avian pathogenic Escherichia coli (APEC) causes significant economic losses to the poultry industries and also potentially threatens human health. However, the effects of DctR on the fitness and virulence of APEC have not been investigated yet. To assess the function of DctR in APEC, the dctR gene mutant and complemented strains were constructed and biologically characterized. Our results show that inactivation of the dctR gene led to decreased biofilm formation, diminished serum resistance, reduced adherence capacity, attenuated colonization and virulence of APEC in ducks. The altered capacities of the mutant strain were restored by genetic complementation. In addition, we found that DctR positively regulates the expression of E. coli type III secretion system 2 (ETT2) core genes in APEC. The expression of the inflammatory cytokines interleukin (IL)-1β and IL-8 were decreased in HD-11 macrophages infected with the mutant strain compared with the wild-type strain. These observations indicate that regulator DctR contributes to the virulence of APEC through regulation of ETT2 expression.

Pathogenic Escherichia coli

10.1186/s13567-021-00970-6

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Citations (6)

Identification of novel genes essential for Brucella abortus to establish infection by signature-tagged mutagenesis

Veterinary Microbiology (2019)

Mingxing Tian Jing Qu Peng Li Yanqing Bao Jiameng Liu

Transposon mutagenesis

Intracellular parasite

10.1016/j.vetmic.2019.02.005

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Citations (11)

Simultaneous determination of chiral pesticide flufiprole enantiomers in vegetables, fruits, and soil by high-performance liquid chromatography

Analytical and Bioanalytical Chemistry (2015)

Mingxing Tian Qing Zhang Haiyan Shi Beibei Gao Xiude Hua

Matrix (chemical analysis)

Solid phase extraction

10.1007/s00216-015-8543-3

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Citations (30)

Characterization of Mycoplasma gallisepticum pyruvate dehydrogenase alpha and beta subunits and their roles in cytoadherence

PLoS ONE (2018)

Jingjing Qi Fanqing Zhang Yu Wang Ting Liu Lei Tan

Mycoplasma gallisepticum is a causative agent of chronic respiratory disease in chickens, typically causing great economic losses. Cytoadherence is the critical stage for mycoplasma infection, and the associated proteins are important for mycoplasma pathogenesis. Many glycolytic enzymes are localized on the cell surface and can bind the extracellular matrix of host cells. In this study, the M. gallisepticum pyruvate dehydrogenase E1 alpha subunit (PDHA) and beta subunit (PDHB) were expressed in Escherichia coli, and their enzymatic activities were identified based on 2,6-dichlorophenol indophenol reduction. When recombinant PDHA (rPDHA) and recombinant PDHB (rPDHB) were mixed at a 1:1 molar ratio, they exhibited strong enzymatic activity. Alone, rPDHA and rPDHB exhibited no or weak enzymatic activity. Further experiments indicated that both PDHA and PDHB were surface-exposed immunogenic proteins of M. gallisepticum. Bactericidal assays showed that the mouse anti-rPDHA and anti-rPDHB sera killed 48.0% and 75.1% of mycoplasmas respectively. A combination of rPDHA and rPDHB antisera had a mean bactericidal rate of 65.2%, indicating that rPDHA and rPDHB were protective antigens, and combining the two sera did not interfere with bactericidal activity. Indirect immunofluorescence and surface display assays showed that both PDHA and PDHB adhered to DF-1 chicken embryo fibroblast cells and adherence was significantly inhibited by antisera against PDHA and PDHB. Adherence inhibition of M. gallisepticum to DF-1 chicken embryo fibroblast cells was 30.2% for mouse anti-rPDHA serum, 45.1% for mouse anti-rPDHB serum and 72.5% for a combination of rPDHA and rPDHB antisera, suggesting that rPDHA and rPDHB antisera may have synergistically interfered with M. gallisepticum cytoadherence. Plasminogen (Plg)-binding assays further demonstrated that both PDHA and PDHB were Plg-binding proteins, which may have contributed to bacterial colonization. Our results clarified the enzymatic activity of M. gallisepticum PDHA and PDHB and demonstrated these compounds as Plg-binding proteins involved in cytoadherence.

10.1371/journal.pone.0208745

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Citations (22)

Does urbanization intensify regional water scarcity? Evidence and implications from a megaregion of China

Journal of Cleaner Production (2019)

Weifeng Li Xia Hai Lijian Han Jingqiao Mao Mingxing Tian

Scarcity

Virtual water

Water use

10.1016/j.jclepro.2019.118592

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Citations (99)

Brucella Infection Regulates Thioredoxin-Interacting Protein Expression to Facilitate Intracellular Survival by Reducing the Production of Nitric Oxide and Reactive Oxygen Species

The Journal of Immunology (2019)

Hai Hu Mingxing Tian Peng Li Xiang Guan Zhengmin Lian

Thioredoxin-interacting protein (TXNIP) is a multifunctional protein that functions in tumor suppression, oxidative stress, and inflammatory responses. However, how TXNIP functions during microbial infections is rarely reported. In this study, we demonstrate that Brucella infection decreased TXNIP expression to promote its intracellular growth in macrophages by decreasing the production of NO and reactive oxygen species (ROS). Following Brucella abortus infection, TXNIP knockout RAW264.7 cells produced significantly lower levels of NO and ROS, compared with wild-type RAW264.7 cells. Inducible NO synthase (iNOS) inhibitor treatment reduced NO levels, which resulted in a dose-dependent restoration of TXNIP expression, demonstrating that the expression of TXNIP is regulated by NO. In addition, the expression of iNOS and the production of NO were dependent on the type IV secretion system of Brucella Moreover, Brucella infection reduced TXNIP expression in bone marrow-derived macrophages and mouse lung and spleen. Knocked down of the TXNIP expression in bone marrow-derived macrophages increased intracellular survival of Brucella These findings revealed the following: 1) TXNIP is a novel molecule to promote Brucella intracellular survival by reducing the production of NO and ROS; 2) a negative feedback-regulation system of NO confers protection against iNOS-mediated antibacterial effects. The elucidation of this mechanism may reveal a novel host surveillance pathway for bacterial intracellular survival.

10.4049/jimmunol.1801550

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Citations (14)

Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR

Agricultural Sciences in China (2011)

Yanfeng Fu Jinluan Fu Yang Lu Mingxing Tian Wen-cheng CHEN

Ephrin

Conceptus

Placentation

EPH receptor A2

10.1016/s1671-2927(11)60137-5

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Citations (3)