Ketrin C. Silva

Universidade de São Paulo

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Nilton Lincopán

Universidade de São Paulo

Andréa Micke Moreno

Universidade de São Paulo

Miriam R. Fernandes

Universidade de São Paulo

Fernanda Esposito

Universidade de São Paulo

Louise Cerdeira

Liverpool School of Tropical Medicine

Terezinha Knöbl

Universidade de São Paulo

Patrícia Neves

Universidade do Porto

Mariza Landgraf

Universidade de São Paulo

Doroti de Oliveira Garcia

Instituto Adolfo Lutz

Elsa Masae Mamizuka

Universidade de São Paulo

Cooperative Institutions

Universidade de São Paulo

Hospital Universitário da Universidade de São Paulo

Universidade Cidade de São Paulo

Universidade Brasil

Instituto Butantan

Universidade Federal de São Paulo

Instituto Adolfo Lutz

Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia

Instituto de Ciências Farmacêuticas

Universidade Estadual Paulista (Unesp)

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Silent dissemination of colistin-resistant Escherichia coli in South America could contribute to the global spread of the mcr-1 gene

Eurosurveillance (2016)

Miriam R. Fernandes Quézia Moura Luciana Sartori Ketrin C. Silva Marcos PV Cunha

During a Brazilian multicentric antimicrobial resistance surveillance study, colistin resistance was investigated in 4,620 Enterobacteriaceae isolated from human, animal, food and environmental samples collected from 2000 to 2016. We present evidence that mcr-1-positive Escherichia coli has been emerging in South America since at least 2012, supporting a previous report on the possible acquisition of mcr-1-harbouring E. coli by European travellers visiting Latin American countries.

MCR-1

Colistin

10.2807/1560-7917.es.2016.21.17.30214

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Citations (215)

First Characterization of CTX-M-15-Producing Escherichia coli Strains Belonging to Sequence Type (ST) 410, ST224, and ST1284 from Commercial Swine in South America

Antimicrobial Agents and Chemotherapy (2016)

Ketrin C. Silva Marina Moreno Carlos Cabrera Beny Spira Louise Cerdeira

ABSTRACT We report for the first time the isolation of CTX-M-15-producing Escherichia coli strains belonging to sequence type (ST) 410, ST224, and ST1284 in commercial swine in Brazil. The bla CTX-M-15 gene was located on F-::A9::B1 and C1::A9::B1 IncF-type plasmids, surrounded by a new genetic context comprising the IS 26 insertion sequence truncated with the IS Ecp1 element upstream of bla CTX-M-15 . These results reveal that commercial swine have become a new reservoir of CTX-M-15-producing bacteria in South America.

Sequence (biology)

Isolation

Insertion sequence

10.1128/aac.02788-15

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Citations (46)

Identification of the cfr methyltransferase gene in Enterococcus faecalis isolated from swine: First report in Brazil

Journal of Global Antimicrobial Resistance (2017)

Pedro H. Filsner Lara Mendes de Almeida Marina Moreno Luisa Zanolli Moreno Carlos E. C. Matajira

Resistome

Enterococcus faecalis

genomic DNA

10.1016/j.jgar.2017.02.002

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Citations (7)

CTX-M-15-producing Escherichia coli ST 648 from human and novel ST belonging to 206 CC from commercial swine samples

Ketrin C. Silva Fernanda N. C. Vasconcelos Andyara L. P. B. Camargo Andréa Micke Moreno Lara Mendes de Almeida

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Detection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potential

Journal of Clinical Microbiology (2017)

Fernanda Esposito Miriam R. Fernandes Ralf Lopes Maria E. Muñoz Caetano P. Sabino

The emergence and rapid dissemination of colistin-resistant Escherichia coli carrying the plasmid-mediated mcr-1 gene have created an urgent need to develop specific screening methods. In this study, we evaluated four assays based on the inhibition of MCR-1 activity by EDTA: (i) a combined-disk test (CDT) comparing the inhibition zones of colistin and colistin (10 μg) plus EDTA (100 mM); (ii) reduction of colistin MIC (CMR) in the presence of EDTA (80 μg/ml); (iii) a modified rapid polymyxin Nordmann/Poirel test (MPNP); and (iv) alteration of zeta potential (RZP = ZP+EDTA/ZP-EDTA). We obtained encouraging results for the detection of MCR-1 in E. coli isolates recovered from human, food, and animal samples, using the following assay parameters: ≥3 mm difference in the inhibition zones between colistin disks without and with EDTA; ≥4-fold colistin MIC decrease in the presence of EDTA; RZP of ≥2.5; and the absence of metabolic activity and proliferation, indicated by unchanged color of phenol red in the presence of colistin-EDTA, in the MPNP test. In this regard, the CDT, CMR, RZP, and MPNP assays exhibited sensitivities of 96.7, 96.7, 95.1, and 96.7% and specificities of 89.6, 83.3, 100, and 100%, respectively, for detecting MCR-1-positive E. coli Our results demonstrate that inhibition by EDTA and zeta potential assays may provide simple and inexpensive methods for the presumptive detection of MCR-1-producing E. coli isolates in human and veterinary diagnostic laboratories.

Colistin

MCR-1

Polymyxin

Zeta potential

Growth inhibition

10.1128/jcm.00835-17

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Citations (53)

Suppurative peritonitis by Klebsiella pneumoniae in captive gold-handed tamarin (Saguinus midas midas )

Journal of Medical Primatology (2015)

Maria Flávia Lopes Guerra Rodrigo Hidalgo Friciello Teixeira Vanessa L. Ribeiro Marcos Paulo Vieira Cunha Maria Gabriela Xavier de Oliveira

Abstract This report describes an outbreak of suppurative peritonitis caused by Klebsiella pneumoniae in an adult female of captive golden‐handed tamarin ( Saguinus midas midas ). Two virulent and multidrug‐resistant strains were isolated and classified through MLST as ST 60 and ST 1263. The microbiological diagnosis works as a support tool for preventive measures.

Klebsiella

10.1111/jmp.12202

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Citations (7)

Low-virulence phylogenetic background of CTX-M-producing Escherichia coli isolated from extraintestinal infections

The Journal of Infection in Developing Countries (2013)

Andyara L. Paiva Nilton Lincopán Ketrin C. Silva Patrícia Neves Andréa Micke Moreno

This item has no abstract. Follow the links below to access the full text.

10.3855/jidc.3781

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Isolation of Pseudomonas aeruginosa Coproducing Metallo-β-Lactamase SPM-1 and 16S rRNA Methylase RmtD1 in an Urban River

Antimicrobial Agents and Chemotherapy (2011)

Lívia C. Fontes Patrícia Neves Silvane Oliveira Ketrin C. Silva Elayse Maria Hachich

Pseudomonas aeruginosa (strain 48-1997A) producing Sao Paulo metallo-beta-lactamase (MBL)-1 (SPM-1) was described for the first time in 1997 and came from a 4-year-old leukemic girl in Sao Paulo, Brazil (15). Since then, SPM-1-producing P. aeruginosa has become endemic in Brazilian hospitals, being recurrently associated with outbreaks of nosocomial infection (8, 11, 12). More recently, the first reports of the MBL SPM-1 were described in Europe (14) and western Asia (GenBank accession no. {type:entrez-nucleotide,attrs:{text:HM370523.1,term_id:307949947,term_text:HM370523.1}}HM370523.1), denoting the potential for SPM-1 to become endemic worldwide (6). Unfortunately, carbapenem-resistant P. aeruginosa isolates carrying the SPM-1 enzyme have exhibited additional resistance to aminoglycosides, which has been mediated by a novel 16S rRNA methylase defined as RmtD (3), rendering ineffective a potent double-coverage regimen of a carbapenem plus an aminoglycoside and contributing to the emergence of panresistant phenotypes (3, 4). In this letter, we report the first environmentally isolated Pseudomonas aeruginosa carrying the blaSPM-1 and rmtD1 genes, providing additional data on the epidemiology of these genetic determinants of resistance. In August of 2010, during a surveillance study established to monitor the occurrence of antimicrobial resistance in Gram-negative bacteria from urban rivers in southeastern Brazil, a panresistant P. aeruginosa isolate (TIES-04900) was recovered from the Tiete River. Tiete is one of the main rivers of the region, which runs across Sao Paulo state from east to west for about 1,100 km. The water sample was collected from a dam located downstream of many important cities, including Sao Paulo, the largest and most populous metropolitan area in Brazil. P. aeruginosa strain TIES-04900 was resistant to imipenem, meropenem, ertapenem, ceftazidime, cefotaxime, cefepime, piperacillin-tazobactam, amikacin, tobramycin, gentamicin, ciprofloxacin, levofloxacin, and trimethoprim-sulfamethoxazole; remaining susceptible only to aztreonam and polymyxin B (Table 1). MBL production was confirmed by using an MBL Etest strip. Based on the antimicrobial susceptibility pattern and genetic backgrounds of multidrug-resistant P. aeruginosa strains reported from Brazil (4, 8), antibiotic resistance genes were studied by PCR and sequencing, confirming the presence of the blaSPM-1, blaOXA-56, rmtD1, aacA4, aadA7, sul1, and dhfr genes (GenBank accession numbers {type:entrez-nucleotide,attrs:{text:HQ876773,term_id:323099809,term_text:HQ876773}}HQ876773 to {type:entrez-nucleotide,attrs:{text:HQ876777,term_id:323099817,term_text:HQ876777}}HQ876777). This resistance genotype has been previously reported in clinical isolates of P. aeruginosa (3) and most likely has contributed to the endemicity of this panresistant phenotype in Brazilian hospitals (3, 4, 6, 8, 11, 12). In fact, enterobacterial repetitive intergenic consensus-PCR fingerprint analysis (Dice similarity coefficient and unweighted-pair group method using average linkages clustering), undertaken to ascertain the relatedness of TIES-04900 to clinical SPM-1- and RmtD-1-positive P. aeruginosa isolates (3, 15), showed that environmental P. aeruginosa TIES-04900 was clonally related to human P. aeruginosa strains PA0905 (95.2% similarity) and 48-1997A (90% similarity), previously identified in Sao Paulo (3, 15). Table 1. MICs of antibiotics for environmental Pseudomonas aeruginosa strain TIES-04900 carrying the blaSPM-1, blaOXA-56, rmtD1, aacA4, aadA7, sul1, and dhfr genesa In summary, we report the presence of SPM-1- and RmtD-1-coproducing P. aeruginosa in an urban river, showing that strains harboring the blaSPM-1 and rmtD1 genes seem not to be restricted to the hospital setting, a fact that emphasizes the importance of surveying environmental strains that might act as a source and/or reservoir of resistance genes with clinical relevance (1, 5, 7, 13) and have the potential for dissemination in communities through river environments (1, 10).

Isolation

10.1128/aac.00138-11

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Citations (32)

MDR ST2179-CTX-M-15 Escherichia coli co-producing RmtD and AAC(6′)-Ib-cr in a horse with extraintestinal infection, Brazil

Journal of Antimicrobial Chemotherapy (2014)

Lucianne Leigue José Francisco Ghignatti Warth Luana C. Melo Ketrin C. Silva Rodrigo Assunção Moura

10.1093/jac/dku520

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Resistência antimicrobiana e sorotipos de Salmonella entérica circulando na produção suína brasileira

Ketrin C. Silva Luciane Tieko Shinya Zucon Vasco Túlio de Moura Gomes Denis Hideki Nakasone Thaís Sebastiana Porfida Ferreira

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