Neuroblastoma is a common malignancy and frequently affects children, leading to a low survival rate. Long noncoding RNAs (lncRNAs) are reported to be closely related to cancer progression. The purpose of this study was to explore a novel mechanism of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in neuroblastoma. NEAT1 was upregulated in neuroblastoma cell lines (IMR32 and SK-N-SH). Overexpression of NEAT1 increased proliferation inhibited by cisplatin and decreased apoptosis promoted by cisplatin. MicroRNA-326 (miR-326) was a target of NEAT1 and miR-326 reintroduction abolished the effects of NEAT1 overexpression on cell proliferation and apoptosis. Moreover, NEAT1 overexpression activated Janus kinase/signal transducer and activator of transcription 3 (JAK1/STAT3) signaling pathway through absorbing miR-326. Besides, NEAT1 overexpression promoted tumor growth in vivo through stimulating the expression of p-JAK1 and p-STAT3 but inhibiting miR-326 expression. NEAT1 accelerated proliferation and weakened apoptosis of neuroblastoma cells treated by cisplatin by targeting miR-326 through activating JAK1/STAT3 signaling pathway, suggesting that NEAT1 was a potential biomarker against neuroblastoma.
Objective
To explore the effects of virtual teaching methods on intensive care skills teaching.
Methods
A total of 262 nursing students were randomly divided into two groups: intervention group (n=131) and control group (n=131). The patients of two groups were received traditional experimental teaching methods and intervention group also received the virtual teaching methods. The scores of course evaluation, general self-efficacy and critical thinking ability were evaluated.
Results
In the intervention group, the scores of course evaluation, self-efficacy and critical thinking ability were (84.12±5.32), (2.72±0.35) and (293.21±5.71) which were better than those in the control group (t=-8.47, -11.66, -15.45; P<0.01).
Conclusions
The virtual teaching methods can stimulate learning interests of students and improve the scores of course evaluation, self-efficacy and critical thinking ability.
Key words:
Education, nursing; Intensive care unit; Self-efficacy; Critical thinking mind; Virtual teaching method
In this paper, corn stalk adsorbents are prepared by pretreated corn stalks and carboxymethylation reaction. Then the adsorbents are used to remove Pb2+ in the aqueous solution, and the effect of the pH value of the solution, adsorption time, adsorbent dosage, the initial concentration of Pb2+ on the adsorption performance, and the adsorption kinetics and adsorption isotherm are discussed. The results show that when the pH is 4.50, the adsorption time is 150 min, the amount of adsorbent is 0.45 g, the adsorption capacity of the adsorbent is as high as 928 mg/g, and the Pb2+ removal rate reaches 99.78 %. The adsorption kinetics showed that the adsorption of Pb2+ by the adsorbent reached adsorption equilibrium within 150 minutes, the adsorption process conformed to the quasi-second-order kinetic model, and the adsorption mechanism was chemical adsorption. The adsorption isotherm shows that the adsorption process of Pb2+ by the adsorbent conforms to the single-layer adsorption of the Langmuir model.
In this study, four Theaceae tree species were processed by setting up two hydrogen fluoride concentration gradient (including controls), the actual quantum yield (Y (II)) of PSII, the relative electron transport rate (ETR), non-regulatory energy dissipation quantum yield (Y(NO)), regulatory energy dissipation quantum yield (Y(NPQ)), non-photochemical quenching coefficient (NPQ) and the relative electrical conductivity and other physiological characteristic value were measured, and fuzzy membership function method was applied for comprehensive evaluation and stress resistance sequencing of these four species, which could help to explore the impact of HFstress on physiological indicators of Theacea tree seedlings, and effectively screen out tress species with high resistance to hydrogen fluoride; besides, it could provide the basis for selection of planting trees with the purpose of environmental protection, and also provide a reference for HF stress mechanisms study.This study showed that, under the circumstance of 500 ppm hydrogen fluoride stress, chlorophyll fluorescence indicator of Theaceae tree species presented a downward trend on the whole, which concretely embodied in varying degrees of decline of Y (II) of PSII, ETR, Y (NO), Y (NPQ), NPQ.And the cell membranes of trees species were damaged and the membrane permeability increased.Except for Schima superba, the relative conductivity value of other species showed an upward trend.The capacity of these four species in anti-hydrogen fluoride contamination was Tutcheria championi first, Camellia oleifera second, Schima superba third, Gordonia axillaries is the last.
Objective To summarize prognostic factors influencing the effect of decompressive craniectomy on a large area of ischemic cerebrovascular disease.Methods Retrospective analysis was made of 147 cases of large area of ischemic cerebrovascular disease patients undergoing decompressive craniectomy in clinical efficacy.With causes of death as the end point of the evaluation,analysis was made of the relationship between death and different age,etiology,timing of operation,operation mode,the brain midline shift.Results Fifty-seven patients died after surgery due to increased intracranial lesions,49 cases over 60 died.Combined rheumatic heart disease,infective endocarditis,hypertension resulted in death in 54 cases.The highest is mortality,operative mortality after 48 hours was 47 cases.Forty-eight died with the brain midline shifting more than 1 cm.Conclusion Prognosis of decompressive craniectomy is related with age,etiology,timing and manner of operation,the degree of brain midline shift.
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