Dawid Szczepankiewicz

University of Life Sciences in Poznań

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Ewa Pruszyńska‐Oszmałek

University of Life Sciences in Poznań

Krzysztof W. Nowak

University of Life Sciences in Poznań

Maciej Sassek

University of Life Sciences in Poznań

Paweł Kołodziejski

University of Life Sciences in Poznań

Marek Skrzypski

University of Life Sciences in Poznań

Mathias Z. Strowski

Charité - Universitätsmedizin Berlin

Tatiana Wojciechowicz

University of Life Sciences in Poznań

Aleksandra Szczepankiewicz

Poznan University of Medical Sciences

Przemysław Kaczmarek

University of Wrocław

L. Nogowski

University of Life Sciences in Poznań

Cooperative Institutions

University of Life Sciences in Poznań

Charité - Universitätsmedizin Berlin

Poznan University of Medical Sciences

University of Salzburg

Medical University of Vienna

University of Life Sciences in Lublin

University of Vienna

University of Agriculture in Krakow

German Center for Lung Research

Inserm

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Allergic airway inflammation affects signaling pathways in adipose tissue via mRNA-miRNA interactions in the rat

Dawid Szczepankiewicz W Langwiński J Nowakowska P Kołodziejski Ewa Pruszyńska‐Oszmałek

Previously, we observed that allergic sensitization altered the expression of miRNA profile in adipose tissue. Here, we hypothesized that these changes affect transcriptome in adipose tissue in airway allergic inflammation model. We assessed the gene expression profile adipose tissue in asthma animal model. Brown Norway rats were sensitized intranasally to house dust mite extract for 6 weeks. The control rats received PBS. For transcriptome analysis, Sure Print G3 Rat Gene Expression microarrays were used. Bioinformatic analysis was done using GeneSpring. MicroRNA-mRNA interactions were analyzed in miRTargetLink. Transcriptome in adipose tissue showed that 2263 genes were downregulated and 111 showed increased expression in sensitized rats. The upregulated genes were enriched in 8 pathways: chronic inflammatory response, leukocyte migration, neutrophil chemotaxis. The downregulated genes regulated olfactory transduction, circadian pathway and regulation of insulin secretion. Combining differentially expressed mRNAs with a set of 29 miRNAs, altered in adipose tissue in our previous study, showed that 33 pathways were significantly affected by miRNA-mRNA interactions in adipose tissue during allergic airway inflammation. The most significantly enriched signaling pathways were: PI3K-Akt, cAMP, Ras, neurotrophin, Rap1 and MAPK. Allergic airway inflammation in adipose tissue enhances inflammatory responses, but downregulates olfactory and circadian pathways and also influences several signaling pathways via miRNA-mRNA interactions. The study was supported by National Science Centre in Poland, grant no. 2019/35/B/NZ5/02906 (A.Szczepankiewicz).

Adipose tissue macrophages

10.1183/13993003.congress-2022.2275

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Changes of agouti-related protein in hypothalamus, placenta, and serum during pregnancy in the rat

Journal of Endocrinology (2009)

Dawid Szczepankiewicz Ewa Pruszyńska‐Oszmałek Przemysław Kaczmarek Marek Skrzypski Karolina M. Andralojc

Agouti-related protein (AGRP) is a homolog of the agouti protein and acts as an antagonist of peptides derived from propiomelanocortin through melanocortin receptors. This peptide is produced mainly in the hypothalamus, particularly during negative energy balance and influences increased food intake. In the hypothalamus, this peptide is co-expressed in arcuate nuclei with neuropeptide Y, another important peptide that regulates energy metabolisms. In our study, we analyzed changes in the Agrp mRNA level in the hypothalamus as well as mRNA and protein levels in placenta during different stages of rat pregnancy. We also investigated the AGRP level in the blood serum. In this study, we found the AGRP level in serum increased, while its gene expression in the hypothalamus increased only up to the 13th day of pregnancy, and decreased on the 18th day. This study demonstrates that AGRP is expressed during late pregnancy in placenta. Moreover, we found that AGRP expression is higher on the 18th than on the 13th day of pregnancy. Our results indicate that AGRP may play an important role during pregnancy in the mother's and, possibly, also in the fetus's energy balance.

10.1677/joe-09-0053

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Ghrelin but not obestatin regulates insulin secretion from INS1 beta cell line via UCP2-dependent mechanism.

PubMed (2010)

Joanna Chmielewska Dawid Szczepankiewicz Marek Skrzypski D Kregielska MZ Strowski

The mitochondrial UCP2 mediates glucose-stimulated insulin secretion by decreasing intracellular ATP/ADP ratio. Insulin secretion is a tightly regulated process. Ghrelin, as well as obestatin, were intensively studied to determine their ability to modify insulin secretion. Ghrelin is considered to be an inhibitor of insulin release from pancreatic islets, however little is known about the effects of obestatin. In our study we demonstrate the stimulating effects of both peptides on insulin secretion in INS1 cells. Furthermore, we investigate the potential role of UCP2 in mediating the effects of both peptides on insulin secretion. UCP2 mRNA expression was down-regulated by ghrelin in the presence of 26.4 mM glucose, however it was unchanged after obestatin treatment. Our results confirm that UCP2 could be involved in the stimulating effect of ghrelin on insulin release from INS1 cells.

Obestatin

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Quality and number of cumulus-oocyte complexes (COC) and concentrations of leptin and ghrelin in blood and follicular fluid depending on the body condition of cows

Medycyna Weterynaryjna (2011)

Tomasz Nowak J. M. Jaśkowski Dawid Szczepankiewicz J. Olechnowicz

Follicular fluid

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The Level of Selected Hormones in Peripheral Blood in Female Polar Foxes (Alopex lagopus L.) in Relation to Age

Folia Biologica (2009)

Roman Szymeczko P. Maćkowiak Anna Piotrowska Monika Bogusławska‐Tryk Katarzyna Burlikowska

The objective of the study was to determine the concentration profile of selected hormones in the blood serum of blue polar fox vixens at various ages during the non-mating period, three months after lactation. The investigation was performed on 50 clinically healthy female polar foxes derived from a domestic reproductive farm. The animals were divided into 5 age groups (n = 10) ranging from the 1 to 5 years of life. In the blood serum the contents of insulin, triiodothyronine (total and free), thyroxin (total and free), leptin and ghrelin (total and active) were determined. No significant, female age-dependent differences were found in the contents of insulin, total and free triiodothyronine and total ghrelin in the blood serum. Significant (P < 0.05) differences were observed in the concentration of total and free thyroxin (the highest in the blood of 4-year old females) as well as leptin and active ghrelin (the lowest and the highest content in 3-year old vixens). However, no distinct, female age-dependent tendencies for change in the content of these hormones in the blood serum were observed.

10.3409/fb57_3-4.213-218

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Changes of ghrelin and growth hormone expression in hypothalamus-pituitary axis during pregnacy in the rat

Acta Biochimica Polonica (2006)

Dawid Szczepankiewicz Marek Skrzypski Ewa Pruszyńska‐Oszmałek P. Kaczmarek Tatiana Wojciechowicz

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MOTS-c Impact on Muscle Cell Differentiation and Metabolism Across Fiber Types

Cellular Physiology and Biochemistry (2025)

Natalia Leciejewska Ewa Pruszyńska‐Oszmałek Paweł Kołodziejski Dawid Szczepankiewicz L. Nogowski

Background/Aims: MOTS-c belongs to a group of mitochondrial peptides involved in metabolic processes in the body. This peptide has garnered increasing attention since its discovery in 2015 because of its potential to ameliorate metabolic parameters in animals with diabetes or insulin resistance. MOTS-c is involved in muscle metabolism; however, little is known about its role in fiber differentiation. Materials: We conducted a study to explore the effect of MOTS-c on cellular processes using the C2C12 and L6 cell lines, representing different metabolic types of muscle fibers. The research methods were real-time PCR, Western blot, and lipid accumulation measurement. Results: >Notably, our investigations revealed that MOTS-c increased the survival of C2C12 cells at doses of 10 and 100 nM (p<0.01) and stimulated the phosphorylation of extracellular signal-regulated kinase within 5 min of incubation (p<0.05). Remarkably, these effects were not observed in L6 cells; however, both cell lines showed a reduced rate of proliferation. Furthermore, MOTS-c promotes the differentiation of C2C12 cells by increasing the expression of muscle regulatory factors, but it does not produce such an effect in L6 cells. Additionally, cells were treated with physiological concentrations of free fatty acids and MOTS-c, unveiling an augmentation in lipid accumulation observed in L6 cells and a decrease in lipid accumulation in C2C12 cells. Conclusion: In conclusion, our findings have suggested a diverse response to MOTS-c depending on the type of muscle fibers, particularly in the domains of survival, cell differentiation, and lipid accumulation.

C2C12

10.33594/000000755

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Allergic inflammation in lungs and nasal epithelium of rat model is regulated by tissue-specific miRNA expression

Molecular Immunology (2022)

Wojciech Langwiński Dawid Szczepankiewicz Beata Narożna John Stegmayr Darcy E. Wagner

Allergic Inflammation

10.1016/j.molimm.2022.04.017

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Citations (7)

Orexin A but not orexin B regulates lipid metabolism and leptin secretion in isolated porcine adipocytes

Domestic Animal Endocrinology (2018)

Ewa Pruszyńska‐Oszmałek Paweł Kołodziejski P. Kaczmarek Maciej Sassek Dawid Szczepankiewicz

Lipogenesis

Orexin-A

10.1016/j.domaniend.2017.12.003

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Citations (20)

Circadian pathway is downregulated in lungs and adipose tissue in the rat model of allergic airway inflammation

03.02 - Airway cell biology and immunopathology (2022)

Aleksandra Szczepankiewicz Joanna Nowakowska Wojciech Langwiński Paweł Kołodziejski Ewa Pruszyńska‐Oszmałek

The aim of this study was to compare the transcriptome changes during allergic airway inflammation between the lungs and adipose tissue. In this study, we assessed the gene expression profile in the lungs in asthma animal model. Brown Norway rats were sensitized intranasally to house dust mite extract for 6 weeks. The control rats received PBS intranasally for the same period of time. For the transcriptome expression profiling in adipose tissue, we used Sure Print G3 Rat Gene Expression microarrays. Bioinformatic analysis was done using GeneSpring software. Pathway analysis was done in DAVID tool. Transcriptome profiling in the lungs showed that 179 genes were downregulated, whereas 217 were upregulated in allergic airway inflammation model as compared to the control rats. The downregulated genes were involved in the regulation of 4 pathways: protein processing in endoplasmic reticulum, estrogen signaling pathway, circadian rhythm and Rap1 signaling pathway. Upregulated genes were involved in 23 pathways regulation including: TNF signaling pathway, chemokine signaling pathway, cytokine-cytokine receptor interaction. Comparison of differentially expressed genes between lungs and adipose tissue of allergic rats, showed 31 genes deregulated in both tissues. These genes were significantly involved in the circadian pathway (adj p=0.036), neutrophil aggregation (adj p=0.005) and leukocytes cell-cell adhesion (adj p=0.048). Allergic airway inflammation affects lung transcriptome and downregulates circadian pathway not only in lungs, but also in adipose tissue. The study was supported by the National Science Centre in Poland, grant no. 2019/35/B/NZ5/02906 (A. Szczepankiewicz)

10.1183/13993003.congress-2022.2270

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