Mohd Hafidz Mahamad Maifiah

International Islamic University Malaysia

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Jian Li

Monash University

Tony Velkov

University of Melbourne

Yumi Zuhanis Has-Yun Hashim

International Islamic University Malaysia

Darren J. Creek

Monash University

Yan Zhu

Chinese Academy of Sciences

Muhamad Shirwan Abdullah Sani

International Islamic University Malaysia

Nusaibah Abdul Rahim

University of Malaya

Khairunnisa Mohd Kamal

International Islamic University Malaysia

Brian T. Tsuji

University at Buffalo, State University of New York

Alan Forrest

Royal Botanic Garden Edinburgh

Cooperative Institutions

International Islamic University Malaysia

Monash University

Australian Regenerative Medicine Institute

University of Melbourne

Universiti Putra Malaysia

Discovery Institute

International Islamic University Chittagong

University of Malaya

Chinese Academy of Sciences

Government of Malaysia

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Genome-scale metabolic modeling of responses to polymyxins inPseudomonas aeruginosa

GigaScience (2018)

Yan Zhu Tobias Czauderna Jinxin Zhao Matthias Klapperstueck Mohd Hafidz Mahamad Maifiah

Pseudomonas aeruginosa often causes multidrug-resistant infections in immunocompromised patients, and polymyxins are often used as the last-line therapy. Alarmingly, resistance to polymyxins has been increasingly reported worldwide recently. To rescue this last-resort class of antibiotics, it is necessary to systematically understand how P. aeruginosa alters its metabolism in response to polymyxin treatment, thereby facilitating the development of effective therapies. To this end, a genome-scale metabolic model (GSMM) was used to analyze bacterial metabolic changes at the systems level.A high-quality GSMM iPAO1 was constructed for P. aeruginosa PAO1 for antimicrobial pharmacological research. Model iPAO1 encompasses an additional periplasmic compartment and contains 3022 metabolites, 4265 reactions, and 1458 genes in total. Growth prediction on 190 carbon and 95 nitrogen sources achieved an accuracy of 89.1%, outperforming all reported P. aeruginosa models. Notably, prediction of the essential genes for growth achieved a high accuracy of 87.9%. Metabolic simulation showed that lipid A modifications associated with polymyxin resistance exert a limited impact on bacterial growth and metabolism but remarkably change the physiochemical properties of the outer membrane. Modeling with transcriptomics constraints revealed a broad range of metabolic responses to polymyxin treatment, including reduced biomass synthesis, upregulated amino acid catabolism, induced flux through the tricarboxylic acid cycle, and increased redox turnover.Overall, iPAO1 represents the most comprehensive GSMM constructed to date for Pseudomonas. It provides a powerful systems pharmacology platform for the elucidation of complex killing mechanisms of antibiotics.

Polymyxin

10.1093/gigascience/giy021

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Citations (51)

The Thrombolytic and Cytotoxic Effects of Nigella sativa (L.) Seeds: The Prophetic Medicine

International Journal of Halal Research (2020)

Zahir Uddin Mohammed Babar Irwandi Jaswir Mohd Hafidz Mahamad Maifiah Soraya Ismail Raha Ahmad Raus

The Water-Soluble Extract (WSE) is a crude bioactive phytoconstituent of Nigella sativa (L.) seeds discovered recently. The current findings report about the thrombolytic and cytotoxic effects of WSE using human blood clot lysis and brine shrimp lethality (BSL) bioassay. The thrombolytic effect of WSE (1,666.67 µg/mL) was determined via the clot and lysate weight measurements compared to streptokinase (STK) of 30,000 IU/mL and normal saline (NS) while the cytotoxicity of WSE (44.14-2,000 µg/mL) against vincristine sulfate (VCS;3.125-100 µg/mL). WSE has shown extremely statistically significant (p<0.0001) clot lysis (90.00%) compared to NS (3.76%) whilst it was also significantly different (p<0.0063) to STK (72.41%) exhibiting LC50 of 1,795.90 µg/mL vs. VCS (39.25 µg/mL) in a dose-dependent manner. The current results suggested WSE has a potent thrombolytic effect with mild dose-dependent cytotoxicity towards brine shrimp nauplii (Artemia salina). It also suggested WSE might have enzymatic roles on thrombin, fibrin, and plasmin of blood. This pharmacological action of WSE is might be due to its antioxidant property, short-chain fatty acids and/or amino acids. Further studies are highly recommended on the enzymatic role(s) and bioactive phytoconstituents of WSE.

Brine shrimp

Artemia salina

10.18517/ijhr.2.2.70-77.2020

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Citations (6)

Metabolic Responses to Polymyxin Treatment in Acinetobacter baumannii ATCC 19606: Integrating Transcriptomics and Metabolomics with Genome-Scale Metabolic Modeling

mSystems (2019)

Yan Zhu Jinxin Zhao Mohd Hafidz Mahamad Maifiah Tony Velkov Falk Schreiber

Combating antimicrobial resistance has been highlighted as a critical global health priority. Due to the drying drug discovery pipeline, polymyxins have been employed as the last-line therapy against Gram-negative “superbugs”; however, the detailed mechanisms of antibacterial killing remain largely unclear, hampering the improvement of polymyxin therapy. Our integrative modeling using the constructed genome-scale metabolic model i ATCC19606 and the correlative multiomics data provide the fundamental understanding of the complex metabolic responses to polymyxin treatment in A. baumannii at the systems level. The model i ATCC19606 may have a significant potential in antimicrobial systems pharmacology research in A. baumannii .

Acinetobacter baumannii

Polymyxin

Polymyxin B

10.1128/msystems.00157-18

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Citations (35)

Revisiting an ‘old’ antibiotic class polymyxins by drug combination therapy targeting Gram-negative superbugs: Toyyiban perspective

JOURNAL OF HALAL INDUSTRY & SERVICES (2019)

Mohd Hafidz Mahamad Maifiah Muhamad Shirwan Abdullah Sani Nusaibah Abd Rahim

The Islamic concept of halalan-toyyiban encompasses medicines and pharmaceuticals. Halal pharmaceuticals mean the products are free from any unlawful (haram) constituents while in another aspect, toyyib pharmaceuticals particularly concern on the products’ wholesomeness, quality and safety. Polymyxins, an ‘old’ class antibiotic have been employed as the last-line defence for infections caused by multidrug-resistant (MDR) Gram-negative pathogens. Nevertheless, the exceptional clinical applications of polymyxins are restrained due to their dose-limiting nephrotoxicity and neurotoxicity. In addition, pharmacokinetic/pharmacodynamic (PK/PD) studies indicate that polymyxin monotherapy often fails to achieve an effective in vivo plasma exposure concentration, potentially lead to the emergence of bacterial heteroresistance. To optimise the significant potential of polymyxins, therefore, polymyxin combination therapy has been recommended. The strategy provides some advantages as it significantly confers safer clinical dosage of polymyxins administration thus likely to reduce its toxicity effects. Looking from the toyyiban perspective, this review discusses on the synergistic killing of polymyxin combination for the treatment of MDR Gram-negative infection to reduce its potential toxicity and emergence of resistance.

Polymyxin

Colistin

Source

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Isolation and Identification of Bacteria-producing Cellulose from Tropical Fruit for Halal Capsule Application

International Journal on Advanced Science Engineering and Information Technology (2018)

Nursyahidatul Azwa Awang Azura Amid Mohd Hafidz Mahamad Maifiah Dzun Noraini Jimat

Bacterial cellulose (BC) is pure cellulose synthesized by various species of bacteria. Raising demands on bacterial cellulose is due to its pure and simpler structure. It has plenty of applications in various industries such as food, medical and cosmetics, rendering it a choice in halal industry application as a substitute for non-halal gelatine. However, challenges arise during the BC production such as high production cost and low volumetric yield. In this study, BC is studied to overcome the barriers to BC production. Isolation and identification of cellulose producing bacteria were carried out on eight different tropical fruit sources using Hestrin-Schramm media in static culture condition. Morphological and molecular identification by microscopic observation, gram staining, and 16S rRNA analysis were conducted to identify the characteristics and strain of the new isolates. Next, the selected colonies were challenged to grow in agitating condition using modified HS media. The effects of carbon concentration and agitation speed on the production of cellulose were investigated using on central composite design (CCD). Three new cellulose producing bacteria were successfully isolated and identified to be similar to Enterobacter sp. SJZ-5, Bacterium sp NLAE-zl-H356, and Bulkhoderia sp. RD_DACAR_02 through morphological and molecular analysis. The most potent strain which is similar to Enterobacter sp. SJZ-5 (named as Enterobacter sp.M003) has been chosen for BC optimization study for high BC production using modified HS media. Optimization of bacterial cellulose production using response surface methodology (RSM) with 13 runs indicated that the optimal production parameters were 17.5 g/L for carbon concentration at 277 rpm for agitation speed gave 1.7g/L cellulose. It is expected that the newly isolated bacteria will be able to provide an alternative to gelatine for halal capsule production, thus minimizing and replacing non-halal gelatine usage.

Bacterial Cellulose

Isolation

10.18517/ijaseit.8.4-2.7033

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Citations (3)

BENCHMARKING OF HALAL FOOD PRODUCTS USING SIMILARITY MEASURES â€“ A CONCEPTUAL MODEL

Deleted Journal (2019)

Mohd Izzuddin Mohd Tamrin Sherzod Turaev Mohd Zulfaezal Che Azemin Mohamed Jalaldeen Mohamed Razi Mohd Hafidz Mahamad Maifiah

Muslims are concern with the Halal status of food products sold in the supermarkets. Many products that are imported from overseas are not certified by JAKIM. In this paper, we proposed a conceptual model for benchmarking food products against certified Halal products. Our motivation is to provide similarity measurement between certified and non-certified food products based on their ingredients. This model comprises of three main phases: ingredient acquisition, ingredient transformation and calculate similarity measure. In the first phase, web crawlers are employed to retrieve product information from JAKIM online database and supermarket web pages. In the second phase, an index structure will be constructed to allow faster ingredient retrieval which will be used for similarity calculation. In the last phase, Euclidian distance, cosine similarity measure and Jaccard correlation coefficient will be used to measure the similarities between two products. Our proposed model is to complement but not to replace the existing JAKIM procedure to verify food products by empowering Muslim consumers with informed decision making.

Benchmarking

Jaccard index

Similarity (geometry)

Similarity measure

Ingredient

10.31436/jisdt.v1i1.85

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Citations (0)

Molecular Cloning of Phytase Gene from ASUIA279 and Its Expression in Pichia pastoris System

IIUM Engineering Journal (1970)

Mohd Hafidz Mahamad Maifiah Parveen Jamal Tamrin Nuge Nor Soleha Mohd Dali Anis Shobirin Meor Hussin

Phytases catalyze the hydrolysis of phytate (myo-inositol hexakisphosphate), one of the major storage form of phosphate in plants, with subsequent release of myo-inositol, phosphate and phytate-bound minerals. Non-ruminant animals such as chicken, swine and fish can't use the organic phosphorus and minerals from their diet because there is no phytase activity in their digestive tract. Phytate degrading enzyme is added to the animal feed diet to improve phosphorus availability from the dietary phytate and at the same time this lessen the phosphate pollution level in areas of intensive animal production as the phosphate would not be excreted out to the environment. ASUIA279, a bacterial strain isolated from Malaysian soil has potentially shown good phytase activity. In the present work, the gene encoding for phytase has been amplified from the plasmid DNA of recombinant ASUIA279(5) obtained from a previous study (unpublished data) by a polymerase chain reaction (PCR) methodology. The amplified phytase gene was extracted, purified then cloned into the pPICZï¡A plasmid and transformed into Pichia pastorisX-33 strain for enzyme production.ABSTRAK: Fitase pemangkinan hidrolisis fitat (myo-inositol hexakisphosphate), merupakan salah satu cara penyimpanan utama fosfat dalam tumbuhan, dengan pelepasan berturut myo-inositol , fosfat dan galian terikat fitat. Haiwan bukan ruminan seperti ayam, khinzir dan ikan tidak dapat memanfaatkan fosforus organik serta galian yang diperolehi daripada makanan kerana tidak mempunyai aktiviti fitase di dalam saluran pencernaan mereka. Enzim pengecilan fitase dicampurkan ke dalam pemakanan haiwan untuk mempertingkatkan keperolehan fosforus dari fitat diet. Pada masa yang sama ia dapat mengurangkan tahap pencemaran fosfat di kawasan yang terdapat penternakan haiwan secara intensif agar fosfat tidak dikumuhkan ke persekitaran. ASUIA279, satu strain bakteria yang diasingkan daripada tanih di Malaysiamenunjukkan aktiviti fitase yang memberansangkan. Buat masa kini, pengekodan gen fitase telah diperkuatkan dengan plasmid DNA dari ASUIA279(5), rekombinan yang diperolehi daripada kajian terdahulu (data tak diterbitkan) berdasarkan kaedah tindak balas berantai polimerase (polymerase chain reaction (PCR)). Gen fitase yang diperkuatkan, ditulenkan dan kemudian diklonkan menjadi plasmid pPICZαA dan berubah menjadi strain Pichia pastorisX-33 untuk penghasilan enzim.KEYWORDS: Phytase, animal feed, pollution, polymerase chain reaction (PCR), Pichia pastoris

Phytase

10.31436/iiumej.v12i4.219

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Citations (0)

Porcine And Bovine-Derived Ingredients: Islamic Rules in Halal Pharmaceutical Products

Journal of Fatwa Management and Research (2023)

Mohd Hafidz Mahamad Maifiah Wan Nur Ezzah Syahirah Wan Ismail

For Muslims, ingesting or utilising any products derived from porcine is forbidden as it is considered unclean. Besides, although bovine is halal to be consumed, this ruling is only applicable if it is slaughtered strictly according to Islamic law (Shari’ah). In view of Islam’s imposing certain restrictions on the consumption of porcine- and bovine-based products, for Muslims who are seeking medication, this could limit their therapeutic options as many pharmaceutical products are sourced from both animals. This will eventually make it difficult for them to get treatment and will endanger their lives. Hence, it is crucial for every Muslim to have knowledge and understanding about the views of Islamic jurists (Fuqaha’) regarding the Islamic rules on pharmaceutical products containing porcine- and bovine-derived ingredients. In this article, a clear narration regarding porcine and bovine-derived pharmaceutical products, accompanied by a review of Islamic rules based on Islamic jurists, is presented.

10.33102/jfatwa.vol28no3.549

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Citations (1)

Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis

Proceedings of the National Academy of Sciences (2016)

Md Saruar Bhuiyan Felix Ellett Gerald L. Murray Xenia Kostoulias Gustavo M. Cerqueira

Significance Acinetobacter baumannii is one of the most significant hospital-acquired bacterial pathogens, able to cause life-threatening infections and develop resistance to all currently available antibiotic agents. Here, we established zebrafish as a model to study real-time interactions between innate immune cells and A. baumannii during infection. We identified a bacterial metabolic pathway that, when inhibited, leads to enhanced immune responses toward the bacteria, improving bacterial clearance and reducing severity of disease. The enhanced immune response was secondary to accumulation of a metabolic by-product, which acted as a direct, bacterial-mediated attractant of neutrophils, the key immune cell important in response to bacterial infections. These results pave the way for novel therapeutic targeting of bacterial metabolism to stimulate immune responses to fight off infection.

Acinetobacter baumannii

10.1073/pnas.1523116113

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Citations (108)

Exogenous metabolite feeding on altering antibiotic susceptibility in Gram-negative bacteria through metabolic modulation: a review

Metabolomics (2022)

Wan Yean Chung Yan Zhu Mohd Hafidz Mahamad Maifiah Naveen Kumar Hawala Shivashekaregowda Eng Hwa Wong

Microbial Metabolism

Bacterial growth

10.1007/s11306-022-01903-w

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Citations (9)