José Antonio Tercero

Centro de Biología Molecular Severo Ochoa

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Irene Saugar

Centro de Biología Molecular Severo Ochoa

Rosa Ana Lacalle

Centro Nacional de Biotecnología

John F.X. Diffley

The Francis Crick Institute

Carl P. Lehmann

Centro de Biología Molecular Severo Ochoa

Antonio Jiménez

Instituto de Investigación Biosanitaria de Granada

Juan Carlos Espinosa

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

MÁ

María Ángeles Ortiz-Bazán

Centro Andaluz de Biología Molecular y Medicina Regenerativa

M. Inmaculada Barrasa

Whitehead Institute for Biomedical Research

Alberto Jiménez-Martín

Instituto de Biología Funcional y Genómica

María Victoria Vázquez

Ministerio de Salud

Cooperative Institutions

Universidad Autónoma de Madrid

Centro de Biología Molecular Severo Ochoa

Consejo Superior de Investigaciones Científicas

IFOM

University of Geneva

Friedrich Miescher Institute

Cancer Research UK

Universidad de Sevilla

Universidad de Salamanca

Centro Nacional de Biotecnología

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Encoding genes for endosperm proteins in Hordeum chilense

Theoretical and Applied Genetics (1991)

José Antonio Tercero Angeles Bernardo N. Jouve

Prolamin

Hordeum

Triticum turgidum

Hordein

10.1007/bf00226122

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Citations (18)

Cell cycle-dependent regulation of the nuclease activity of Mus81–Eme1/Mms4

Nucleic Acids Research (2012)

María Gallo-Fernández Irene Saugar María Ángeles Ortiz-Bazán María Victoria Vázquez José Antonio Tercero

The conserved heterodimeric endonuclease Mus81–Eme1/Mms4 plays an important role in the maintenance of genomic integrity in eukaryotic cells. Here, we show that budding yeast Mus81–Mms4 is strictly regulated during the mitotic cell cycle by Cdc28 (CDK)- and Cdc5 (Polo-like kinase)-dependent phosphorylation of the non-catalytic subunit Mms4. The phosphorylation of this protein occurs only after bulk DNA synthesis and before chromosome segregation, and is absolutely necessary for the function of the Mus81–Mms4 complex. Consistently, a phosphorylation-defective mms4 mutant shows highly reduced nuclease activity and increases the sensitivity of cells lacking the RecQ-helicase Sgs1 to various agents that cause DNA damage or replicative stress. The mode of regulation of Mus81–Mms4 restricts its activity to a short period of the cell cycle, thus preventing its function during chromosome replication and the negative consequences for genome stability derived from its nucleolytic action. Yet, the controlled Mus81–Mms4 activity provides a safeguard mechanism to resolve DNA intermediates that may remain after replication and require processing before mitosis.

Nuclease

10.1093/nar/gks599

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Citations (115)

Multiple pathways cooperate to facilitate DNA replication fork progression through alkylated DNA

DNA repair (2008)

María Victoria Vázquez Vanesa Rojas José Antonio Tercero

DNA re-replication

Origin recognition complex

Pre-replication complex

Replication protein A

10.1016/j.dnarep.2008.06.014

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Citations (39)

A Central Role for DNA Replication Forks in Checkpoint Activation and Response

Molecular Cell (2003)

José Antonio Tercero Maria Pia Longhese John F.X. Diffley

Checkpoint Kinase 2

CHEK1

DNA re-replication

Origin recognition complex

10.1016/s1097-2765(03)00169-2

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Citations (387)

The Ard1 Gene from Streptomyces capreolus Encodes a Polypeptide of the ABC-Transporters Superfamily Which Confers Resistance to the Aminonucleoside Antibiotic A201A

European Journal of Biochemistry (1995)

M. Inmaculada Barrasa José Antonio Tercero Rosa Ana Lacalle Antonio Jiménez

Capreolus

10.1111/j.1432-1033.1995.0562m.x

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Citations (6)

Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage

Cell Reports (2017)

Irene Saugar Alberto Jiménez-Martín José Antonio Tercero

10.1016/j.celrep.2017.07.059

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Citations (23)

Coping with and Recovering from Hydroxyurea-induced Replication Fork Arrest in Budding Yeast

Cold Spring Harbor Symposia on Quantitative Biology (2000)

John F.X. Diffley Kristine Bousset Karim Labib Elizabeth Noton Corrado Santocanale

During S phase, the activity of large numbers of replicons must be regulated and coordinated to ensure that thegenome is replicated accurately and quickly (Pasero andSchwob 2000). Replication origins are not all activatedtogether at the beginning of S phase, but they are insteadactivated at different times throughout S phase accordingto a predetermined cell-type-specific program (Fangmanand Brewer 1992; Brewer et al. 1993). Thus, at any giventime, there may be initiation, elongation, and terminationreactions occurring simultaneously in the same nucleus...

Replicon

Budding

Budding yeast

S phase

Replication

Fork (system call)

10.1101/sqb.2000.65.333

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Citations (11)

Density Transfer as a Method to Analyze the Progression of DNA Replication Forks

Methods in molecular biology (2009)

José Antonio Tercero

Replicon

Replication

Origin recognition complex

Replication factor C

Semiconservative replication

Licensing factor

Pre-replication complex

10.1007/978-1-60327-815-7_11

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Citations (2)

Cosmid pJAR4, a novel Streptomyces-Escherichia coli shuttle vector for the cloning of Streptomyces operons

FEMS Microbiology Letters (1992)

José Antonio Tercero

A novel shuttle cosmid vector (pJAR4), based on pK505, was constructed for the cloning of Streptomyces DNA. It is a low-copy-number vector which determines hygromycin B-resistance as a selective marker and was used to clone the puromycin biosynthesis pathway from Streptomyces alboniger. Cosmids pJAR4 and pKC505 (which determines apramycin-resistance) stably co-transform both Streptomyces lividans and Streptomyces griseofuscus.

Cosmid

Shuttle vector

Cloning vector

Cloning (programming)

Multiple cloning site

10.1016/0378-1097(92)90404-c

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Citations (7)

Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression

Science (2000)

Karim Labib José Antonio Tercero John F.X. Diffley

Little is known about the DNA helicases required for the elongation phase of eukaryotic chromosome replication. Minichromosome maintenance (MCM) protein complexes have DNA helicase activity but have only been functionally implicated in initiating DNA replication. Using an improved method for constructing conditional degron mutants, we show that depletion of MCMs after initiation irreversibly blocks the progression of replication forks in Saccharomyces cerevisiae . Like the Escherichia coli dnaB and SV40 T antigen helicases, therefore, the MCM complex is loaded at origins before initiation and is essential for elongation. Restricting MCM loading to the G 1 phase ensures that initiation and elongation occur just once per cell cycle.

Minichromosome maintenance

dnaB helicase

Origin recognition complex

Pre-replication complex

S phase

Replication factor C

10.1126/science.288.5471.1643

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Citations (617)