Zhang Miaobin

South China Normal University

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Cultivation of Ganoderma lucidum using Mango sawdust in Zhuhai

Zhongguo gua-cai (2013)

Chen Ji-min Pan Lijing Zhang Miaobin Hanguo Shen Huang CongLing

Mango sawdust mixed with cotton seed hull and wheat bran were used to growing Ganoderma lucidum.The results showed that the Mango sawdust was suitable component of medium for growing Ganoderma lucidum.The optimal formula of cultivation medium for Ganoderma lucidum was:Mango sawdust (particles with diameter≤5mm) 69%,cotton seed hull 10%,wheat bran 20%,calcium sulfate 1%,and water 60%.

Sawdust

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AGROBACTERIUM 介導石の遺伝的形質転換に関する研究【JST・京大機械翻訳】

Yuanyi Xuebao (2008)

Zhang Miaobin Qingzhong Liang Xiao Hao Peng Cen Fan Ganqun

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Morphogenesis of plant cell

Xibei zhiwu xuebao (2004)

Zhang Miaobin Wang Xiaozuo

从控制细胞形态建成的通用机制,影响形态建成的因素和形态建成的调节3个方面介绍近年来植物细胞形态建成的进展.细胞壁组分和结构的修饰改变是细胞形态建成的关键;细胞骨架的组装和活性,以及膨压的变化对于细胞的形态建成有着重要的作用.

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Advances in Study on Abscisic Acid in Seeds

Journal of Xuzhou Normal University (2004)

Hong Keqian Zhang Miaobin

The main activities of abscisic acid in seeds are abscisic acid synthesis,catabolism,transport and response.Abscisic acid levels,the specific enzyme and the transcription factor in signal transduction pathway of abscisic acid,the relation between abscisic acid and dormancy of seeds are reviewed in this paper.

Catabolism

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Construction of Vector Carrying aiiA Gene of Bacillus thuringiensis and Transformation of Dendrobium

Acta Scientiarum Naturalium Universitatis Sunyatseni (2009)

Pan Lijing Zhang Miaobin Qingzhong Liang Fan Ganqun Cheng Ping

Soft rot disease is a great threat to the cultivation and production of Dendrobium and other orchids.Bacillus thuringiensis(Bt) contains aiiA gene encoding AiiA protein which could enable plants resistance to the soft rot disease.aiiA gene was amplified from Bt and constructed into the binary vector pCAMBIA1301 to construct the expression vector pSAN.For the first time,the aiiA gene was transferred into Dendrobium by Agrobacterium tumefacien EHA105.Through 4-month selection cultivation,the hygromycin-resistant shoots regenerated from PLBs.Subjected to GUS assay and PCR analysis,the shoots were proved to be transformed shoots.This research work may contribute to cultivate novel Dendrobium species which could resistant to soft rot disease.

Dendrobium

Bacillus thuringiensis

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Cloning,Sequencing and Prokaryotic Expression of dfr from Dendrobium

Acta Horticulturae Sinica (2010)

Pan Lijing Zhang Miaobin Fan GanQun Weiting Chen You-Pei Cao

Dihydroflavonol 4-Reductase(DFR)plays a key role in the formation of different floral color.By using RT-PCR method,a dfr gene was cloned from Dendrobium and designated as Dendfr.The Dendfr gene was 1 164 bp in length and encoded 352 amino acid residues.Amino acid sequence analysis revealed that Den DFR sequence share homologies of 81%-86% with three species of orchid,and share homologies of 56%-72% with the other species.Den DFR protein contains a reserved NADP domain at the N-terminal and a specific 26 amino acid region of substrate specificity with an asparagine(position 134).The encoding region of Dendfr was constructed into pQE30 vector and effectively expressed the protein in E.coli.These will be helpful for studying molecular mechanism of floral color formation and genetic improvement of the Dendrobium.

Dendrobium

Cloning (programming)

Sequence (biology)

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