Luiz Gustavo dos Anjos Borges

Helmholtz Centre for Infection Research

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Adriana Giongo

Julius Kühn-Institut

Letícia Marconatto

Pontifícia Universidade Católica do Rio Grande do Sul

Dietmar H. Pieper

Helmholtz Centre for Infection Research

Adolfo García‐Sastre

Icahn School of Medicine at Mount Sinai

Zuzhang Wei

Guangxi University

Florian Krammer

Icahn School of Medicine at Mount Sinai

Martha I. Nelson

National Institutes of Health

Silke Kahl

Helmholtz Centre for Infection Research

Weijian Huang

Guangxi University

Ying Chen

Guangxi University

Cooperative Institutions

Universidade Federal do Rio Grande do Sul

Pontifícia Universidade Católica do Rio Grande do Sul

Icahn School of Medicine at Mount Sinai

Universidade de Brasília

Universidade de Caxias do Sul

University of Rio Grande and Rio Grande Community College

Universidade Federal do Rio Grande

Jena University Hospital

Martin Luther University Halle-Wittenberg

Universidade Federal de Santa Catarina

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Analysis of microbial community biodiversity in activated sludge from a petrochemical plant

Ambiente e Agua - An Interdisciplinary Journal of Applied Science (2021)

Themis Collares Antunes Letícia Marconatto Luiz Gustavo dos Anjos Borges Adriana Giongo Sueli Teresinha Van Der Sand

Theactive sludge process is one of the most-used techniques for the biodegradation of organic compounds present in effluents from an assortment of wastewaters. This study investigated the bacterial community structure of a petroleum industry’s activated sludge and its physical and chemical parameters using high-throughput sequencing. Samples were collected over one year: autumn 2015 (C1), winter 2015 (C2), spring 2015 (C3), and summer 2016 (C4). Total DNA was extracted, and the primers targeting the V4 region of the 16S rRNA gene were used for amplicon sequencing. The majority of the detected microorganisms were considered rare microbiota, presenting a relative abundance below 1% of the total sequences. All of the sequences were classified at the phylum level, and up to 55% of the ASVs (Amplicon Sequence Variants) were associated with known bacterial genera. Proteobacteria was the most abundant phylum in three seasons, while the phylum Armatimonadota dominated in one season. The genus Hyphomicrobium was the most abundant in autumn, winter and summer, and an ASV belonging to the family Fimbriimonadaceae was the most abundant in the spring. Canonical Correspondence Analysis showed that physicochemical parameters of SS, SD and TSS are correlated, as well as ammoniacal nitrogen. Sample C3 presented the highest values of COD, AN and solids (SS, SD and TSS). The highest COD, AN, and solids values are correlated to the high frequency of the phylum Armatimonadota in C3.

Amplicon sequencing

10.4136/ambi-agua.2655

Cite

Citations (8)

Live Visualization of Hemagglutinin Dynamics during Infection by Using a Novel Reporter Influenza A Virus

Viruses (2020)

Luiz Gustavo dos Anjos Borges Giuseppe Pisanelli Oyahida Khatun Adolfo Garcı́a-Sastre Shashank Tripathi

Live visualization of influenza A virus (IAV) structural proteins during viral infection in cells is highly sought objective to study different aspects of the viral replication cycle. To achieve this, we engineered an IAV to express a Tetra Cysteine tag (TC tag) from hemagglutinin (HA), which allows intracellular labeling of the engineered HA protein with biarsenic dyes and subsequent fluorescence detection. Using such constructs, we rescued a recombinant IAV with TC tag inserted in HA, in A/Puerto Rico/8/1934(H1N1) background (HA-TC). This recombinant HA-TC tag reporter IAV was replication-competent; however, as compared to wild type PR8 IAV, it was attenuated in multicycle replication. We confirmed expression of TC tag and biarsenical labeling of HA by immunofluorescence assay in cells infected with an HA-TC tag reporter IAV. Further, we used this reporter virus to visualize HA expression and translocation in IAV infected cells by live confocal imaging. We also tested the utility of the HA-TC IAV in testing chemical inhibitors of the HA translocation. Overall, HA-TC IAV is a versatile tool that will be useful for studying viral life cycle events, virus-host interactions, and anti-viral testing.

Immunofluorescence

Viral life cycle

Live cell imaging

10.3390/v12060687

Cite

Citations (2)

Common and species-specific molecular signatures, networks, and regulators of influenza virus infection in mice, ferrets, and humans

Science Advances (2022)

Christian V. Forst Laura Martin‐Sancho Shashank Tripathi Guojun Wang Luiz Gustavo dos Anjos Borges

Molecular responses to influenza A virus (IAV) infections vary between mammalian species. To identify conserved and species-specific molecular responses, we perform a comparative study of transcriptomic data derived from blood cells, primary epithelial cells, and lung tissues collected from IAV-infected humans, ferrets, and mice. The molecular responses in the human host have unique functions such as antigen processing that are not observed in mice or ferrets. Highly conserved gene coexpression modules across the three species are enriched for IAV infection-induced pathways including cell cycle and interferon (IFN) signaling.

10.1126/sciadv.abm5859

Cite

Citations (8)

Bacterial and viral assemblages in ulcerative colitis patients following fecal microbiota and fecal filtrate transfer

ISME Communications (2024)

Howard Junca Arndt Steube Simon Mrowietz Johannes Stallhofer Marius Vital

Fecal microbiota filtrate transfer is discussed as a safe alternative to fecal microbiota transfer (FMT) to treat ulcerative colitis. We investigated modulation of viral and bacterial composition during fecal microbiota filtrate transfer followed by FMT in six patients with active ulcerative colitis (where clinical activity improved in three patients after filtrate transfer) and combined 16S ribosomal RNA gene amplicon sequencing with a virome analysis pipeline including fast viral particle enrichment and metagenome mapping to detect frequencies of 45,033 reference bacteriophage genomes. We showed that after antibiotic treatment and during filtrate transfer, the bacterial community typically adopted a stable composition distinct to that before antibiotic treatment, with no change toward a donor community. FMT in contrast typically changed the bacterial community to a community with similarity to donor(s). There were no indications of an establishment of predominant donor viruses during filtrate transfer but a remodeling of the virome. In contrast, the establishment of donor viruses during FMT correlated with the predicted hosts established during such transfer. Our approach warrants further investigation in a randomized trial to evaluate larger therapeutic interventions in a comparable and efficient manner.

Fecal bacteriotherapy

10.1093/ismeco/ycae167

Cite

Citations (0)

Prokaryotic and microeukaryotic communities in an experimental rice plantation under long-term use of pesticides

Environmental Science and Pollution Research (2020)

María Pilar Serbent Luiz Gustavo dos Anjos Borges Aline Quadros Letícia Marconatto Lorena Benathar Ballod Tavares

10.1007/s11356-020-10614-5

Cite

Citations (11)

Vaginal and neonatal microbiota in pregnant women with preterm premature rupture of membranes and consecutive early onset neonatal sepsis

BMC Medicine (2023)

Luiz Gustavo dos Anjos Borges Jana Pastuschek Yvonne Heimann K Dawczynski M Bergner

Preterm premature rupture of membranes (PPROM), which is associated with vaginal dysbiosis, is responsible for up to one-third of all preterm births. Consecutive ascending colonization, infection, and inflammation may lead to relevant neonatal morbidity including early-onset neonatal sepsis (EONS). The present study aims to assess the vaginal microbial composition of PPROM patients and its development under standard antibiotic therapy and to evaluate the usefulness of the vaginal microbiota for the prediction of EONS. It moreover aims to decipher neonatal microbiota at birth as possible mirror of the in utero microbiota.As part of the PEONS prospective multicenter cohort study, 78 women with PPROM and their 89 neonates were recruited. Maternal vaginal and neonatal pharyngeal, rectal, umbilical cord blood, and meconium microbiota were analyzed by 16S rRNA gene sequencing. Significant differences between the sample groups were evaluated using permutational multivariate analysis of variance and differently distributed taxa by the Mann-Whitney test. Potential biomarkers for the prediction of EONS were analyzed using the MetaboAnalyst platform.Vaginal microbiota at admission after PPROM were dominated by Lactobacillus spp. Standard antibiotic treatment triggers significant changes in microbial community (relative depletion of Lactobacillus spp. and relative enrichment of Ureaplasma parvum) accompanied by an increase in bacterial diversity, evenness and richness. The neonatal microbiota showed a heterogeneous microbial composition where meconium samples were characterized by specific taxa enriched in this niche. The vaginal microbiota at birth was shown to have the potential to predict EONS with Escherichia/Shigella and Facklamia as risk taxa and Anaerococcus obesiensis and Campylobacter ureolyticus as protective taxa. EONS cases could also be predicted at a reasonable rate from neonatal meconium communities with the protective taxa Bifidobacterium longum, Agathobacter rectale, and S. epidermidis as features.Vaginal and neonatal microbiota analysis by 16S rRNA gene sequencing after PPROM may form the basis of individualized risk assessment for consecutive EONS. Further studies on extended cohorts are necessary to evaluate how far this technique may in future close a diagnostic gap to optimize and personalize the clinical management of PPROM patients.NCT03819192, ClinicalTrials.gov. Registered on January 28, 2019.

Neonatal Sepsis

Ureaplasma

Chorioamnionitis

Vaginal flora

10.1186/s12916-023-02805-x

Cite

Citations (24)

The 2‐methylpropene degradation pathway in Mycobacteriaceae family strains

Environmental Microbiology (2023)

Steffen Helbich Israel Barrantes Luiz Gustavo dos Anjos Borges Dietmar H. Pieper Yevhen Vainshtein

Mycolicibacterium gadium IBE100 and Mycobacterium paragordonae IBE200 are aerobic, chemoorganoheterotrophic bacteria isolated from activated sludge from a wastewater treatment plant. They use 2-methylpropene (isobutene, 2-MP) as the sole source of carbon and energy. Here, we postulate a degradation pathway of 2-methylpropene derived from whole genome sequencing, differential expression analysis and peptide-mass fingerprinting. Key genes identified are coding for a 4-component soluble diiron monooxygenase with epoxidase activity, an epoxide hydrolase, and a 2-hydroxyisobutyryl-CoA mutase. In both strains, involved genes are arranged in clusters of 61.0 and 58.5 kbp, respectively, which also contain the genes coding for parts of the aerobic pathway of adenosylcobalamin synthesis. This vitamin is essential for the carbon rearrangement reaction catalysed by the mutase. These findings provide data for the identification of potential 2-methylpropene degraders.

10.1111/1462-2920.16449

Cite

Citations (2)

Seasonal Physiological Parameters and Phytotelmata Bacterial Diversity of Two Bromeliad Species (Aechmea gamosepala and Vriesea platynema) from the Atlantic Forest of Southern Brazil

Diversity (2019)

Adriana Giongo Renata Medina-Silva Leandro Vieira Astarita Luiz Gustavo dos Anjos Borges Rafaël R. de Oliveira

The ecology of complex microhabitats remains poorly characterized in most tropical and subtropical biomes, and holds potential to help understand the structure and dynamics of different biodiversity components in these ecosystems. We assessed nutritional and metabolic parameters of two bromeliad species (Aechmea gamosepala and Vriesea platynema) at an Atlantic Forest site and used 16S rDNA metabarcoding to survey the microbial communities inhabiting their tanks. We observed that levels of some nutrients (e.g., nitrogen) varied across seasons consistently in both species, while others (e.g., phenolic compounds) presented considerable differences between the two bromeliads. In contrast, patterns of tank microbial diversity did not follow a similar temporal trend. There was extensive variation in microbial composition among samples, which included intra-specific differences but also some consistent differences between the two bromeliads. For example, Citrobacter, Klebsiella and Pantoea presented significantly different abundances in the two species. Interestingly, the dominant bacterial genera in both species included Pseudomonas and Enterobacter, which have been reported to include plant-beneficial species. Overall, our data contribute to the characterization of the nutritional status of Atlantic Forest bromeliads and the composition of their prokaryotic communities, laying the foundation for detailed investigations targeting the ecological interactions between these plants and their associated microbes.

Biome

Atlantic forest

10.3390/d11070111

Cite

Citations (12)

Characterization of swine-origin H1N1 canine influenza viruses

Figshare (2023)

Guojun Wang Luiz Gustavo dos Anjos Borges Daniel Stadlbauer Irene Ramos Maria C. Bermúdez González

Host switch events of influenza A viruses (IAVs) continuously pose a zoonotic threat to humans. In 2013, swine-origin H1N1 IAVs emerged in dogs soon after they were detected in swine in the Guangxi province of China. This host switch was followed by multiple reassortment events between these H1N1 and previously circulating H3N2 canine IAVs (IAVs-C) in dogs. To evaluate the phenotype of these newly identified viruses, we characterized three swine-origin H1N1 IAVs-C and one reassortant H1N1 IAV-C. We found that H1N1 IAVs-C predominantly bound to human-type receptors, efficiently transmitted via direct contact in guinea pigs and replicated in human lung cells. Moreover, the swine-origin H1N1 IAVs-C were lethal in mice and were transmissible by respiratory droplets in guinea pigs. Importantly, sporadic human infections with these viruses have been detected, and preexisting immunity in humans might not be sufficient to prevent infections with these new viruses. Our results show the potential of H1N1 IAVs-C to infect and transmit in humans, suggesting that these viruses should be closely monitored in the future.

10.6084/m9.figshare.8847446

Cite

Citations (0)

Meiofaunal diversity in the Atlantic Forest soil: A quest for nematodes in a native reserve using eukaryotic metabarcoding analysis

Forest Ecology and Management (2019)

Carla Aristonara Müller Leandro de Mattos Pereira Carina Mariani Leite Lopes Juvenil Enrique Cares Luiz Gustavo dos Anjos Borges

Environmental DNA

10.1016/j.foreco.2019.117591

Cite

Citations (27)