Karla Vagnerová

Czech Academy of Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Jiřı́ Pácha

Czech Academy of Sciences

Peter Ergang

Czech Academy of Sciences, Institute of Physiology

Martin Vodička

Czech Academy of Sciences, Institute of Physiology

Petra Klusoňová

Czech Academy of Sciences

Ivan Mikšı́k

University of Pardubice

Pavlína Kvapilová

Czech Academy of Sciences

Petra Hermanová

Czech Academy of Sciences, Institute of Microbiology

Tomáš Hudcovic

Czech Academy of Sciences, Institute of Microbiology

DŠ

Dagmar Šrůtková

Czech Academy of Sciences, Institute of Microbiology

Michal Jágr

Crop Research Institute

Cooperative Institutions

Czech Academy of Sciences

Charles University

Czech Academy of Sciences, Institute of Physiology

University of Hradec Králové

Czech Academy of Sciences, Institute of Microbiology

Institute of Clinical and Experimental Medicine

BOKU University

University College London

Czech Academy of Sciences, Institute of Animal Physiology and Genetics

Czech University of Life Sciences Prague

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Comprehensive Study of the Effect of Oat Grain Germination on the Content of Avenanthramides

Michal Jágr Andreas Hofinger-Horvath Peter Ergang Petra Hlásná Čepková Regine Schönlechner

The chemical profile and the levels of AVNs in oat varieties after germination have been examinated. In the present study, 12 distinct oat varieties were germinated for 0-192 h and a total of 28 AVNs and 3 AVN-hexosides were determined in these samples. Among them, three novel AVNs were synthesized (AVN 1a, AVN 2a, and AVN 2ad), characterized using NMR techniques (1D- and 2D-NMR), and assessed in real samples for the first time. The most abundant AVNs in the samples were AVN 2c, AVN 2p, AVN 2f, and their long-chained analogues AVN 2cd, AVN 2pd, AVN 2fd, together representing 75-85 % of the total AVNs content. The highest total AVN level was observed on average after 48-72 h of germination time and it reached a value 1-1.2 mg/g. Out of 12 investigated oat varieties, CDC Boyer, Diadem, and Rozmar have proved to be the most suitable genotypes for germination.

Content (measure theory)

10.2139/ssrn.4531543

Cite

Citations (2)

The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine

International Journal of Molecular Sciences (2021)

Peter Ergang Karla Vagnerová Petra Hermanová Martin Vodička Michal Jágr

Glucocorticoids (GCs) are hormones that are released in response to stressors and exhibit many activities, including immunomodulatory and anti-inflammatory activities. They are primarily synthesized in the adrenal gland but are also produced in peripheral tissues via regeneration of adrenal 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated the influence of the microbiota on de novo steroidogenesis and regeneration of corticosterone in the intestine of germ-free (GF) and specific pathogen-free mice challenged with a physical stressor (anti-CD3 antibody i.p. injection). In the small intestine, acute immune stress resulted in increased mRNA levels of the proinflammatory cytokines IL1β, IL6 and Tnfα and genes involved in de novo steroidogenesis (Stard3 and Cyp11a1), as well as in regeneration of active GCs from their 11-oxo metabolites (Hsd11b1). GF mice showed a generally reduced transcriptional response to immune stress, which was accompanied by decreased intestinal corticosterone production and reduced expression of the GC-sensitive marker Fkbp5. In contrast, the interaction between stress and the microbiota was not detected at the level of plasma corticosterone or the transcriptional response of adrenal steroidogenic enzymes. The results indicate a differential immune stress-induced intestinal response to proinflammatory stimuli and local corticosterone production driven by the gut microbiota.

Corticosterone

Proinflammatory cytokine

10.3390/ijms22084229

Cite

Citations (22)

Heart Chronic Intermittent Hypoxia Induces 11²-Hydroxysteroid Dehydrogenase in Rat

František Kolář Jiřı́ Pácha Petra Klusoňová Lenka Řeháková Gudrun H. Borchert

Hypoxia

Source

Cite

Citations (0)

Peroxisome proliferator-activated receptor-γ stimulates 11β-hydroxysteroid dehydrogenase type 1 in rat vascular smooth muscle cells

Steroids (2011)

Karla Vagnerová J Loukotová Peter Ergang Jana Musı́lková Ivan Mikšı́k

Peroxisome proliferator

Hydroxysteroid Dehydrogenases

10.1016/j.steroids.2011.02.008

Cite

Citations (6)

Social stress stimulates glucocorticoid regeneration in lymphoid organs

Endocrine Abstracts (2017)

Jiřı́ Pácha Peter Ergang Anna Mikulecká Karla Vagnerová Ivan Mikšı́k

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

10.1530/endoabs.49.ep1082

Cite

Citations (0)

Intestinal inflammation modulates expression of 11β-hydroxysteroid dehydrogenase in murine gut

Journal of Endocrinology (2006)

Karla Vagnerová Miloslav Kverka Petra Klusoňová Peter Ergang Ivan Mikšı́k

The effect of glucocorticoids is controlled at the pre-receptor level by the activity of 11β-hydroxysteroid dehydrogenase (11HSD). The isoform 11HSD1 is an NADP + -dependent oxidoreductase, usually reductase, that amplifies the action of glucocorticoids due to reduction of the biologically inactive 11-oxo derivatives cortisone and 11-dehydrocorticosterone to cortisol and corticosterone. The NAD + -dependent isoform (11HSD2) is an oxidase that restrains the effect of hormones due to 11β-oxidation of cortisol and corticosterone to their 11-oxo derivatives. Although the immunosuppressive and anti-inflammatory effects of glucocorticoids are well known, the relationship between inflammation and local metabolism of glucocorticoids is not well understood. In this study, we demonstrated that colitis induced by dextran sulfate sodium modulates colonic 11HSD1. Experimentally induced intestinal inflammation stimulated colonic NADP + -dependent but not NAD + -dependent 11HSD activity. Colonic 11HSD1 mRNA was increased, whereas 11HSD2 mRNA was not changed. Additional parallel studies revealed a similar pattern of 11HSD1 mRNA induction in mesenteric lymph nodes and intestinal intraepithelial lymphocytes, but not in spleen and peritoneal macrophages. These data suggest that inflammation modulates local metabolism of glucocorticoid and support the notion that pre-receptor regulation of endogenous corticosteroids might play a role in inflammatory processes.

Catabolism

Corticosterone

Hydroxysteroid

10.1677/joe.1.06732

Cite

Citations (15)

Comprehensive Targeted Profiling of Multiple Steroid Classes in Rodent Plasma Using Liquid Chromatography-Mass Spectrometry

Analytica Chimica Acta (2024)

Taťána Gazárková Hana Vlčková Kateřina Plachká Karla Vagnerová Dominika Dubecová

Profiling (computer programming)

10.1016/j.aca.2024.343362

Cite

Citations (1)

Peripheral circadian clocks are diversely affected by adrenalectomy

Figshare (2016)

Matúš Soták Jana Bryndová Peter Ergang Karla Vagnerová Pavlína Kvapilová

Glucocorticoids are considered to synchronize the rhythmicity of clock genes in peripheral tissues; however, the role of circadian variations of endogenous glucocorticoids is not well defined. In the present study, we examined whether peripheral circadian clocks were impaired by adrenalectomy. To achieve this, we tested the circadian rhythmicity of core clock genes (Bmal1, Per1-3, Cry1, RevErbα, Rora), clock-output genes (Dbp, E4bp4) and a glucocorticoid- and clock-controlled gene (Gilz) in liver, jejunum, kidney cortex, splenocytes and visceral adipose tissue (VAT). Adrenalectomy did not affect the phase of clock gene rhythms but distinctly modulated clock gene mRNA levels, and this effect was partially tissue-dependent. Adrenalectomy had a significant inhibitory effect on the level of Per1 mRNA in VAT, liver and jejunum, but not in kidney and splenocytes. Similarly, adrenalectomy down-regulated mRNA levels of Per2 in splenocytes and VAT, Per3 in jejunum, RevErbα in VAT and Dbp in VAT, kidney and splenocytes, whereas the mRNA amounts of Per1 and Per2 in kidney and Per3 in VAT and splenocytes were up-regulated. On the other hand, adrenalectomy had minimal effects on Rora and E4bp4 mRNAs. Adrenalectomy also resulted in decreased level of Gilz mRNA but did not alter the phase of its diurnal rhythm. Collectively, these findings suggest that adrenalectomy alters the mRNA levels of core clock genes and clock-output genes in peripheral organs and may cause tissue-specific modulations of their circadian profiles, which are reflected in changes of the amplitudes but not phases. Thus, the circulating corticosteroids are necessary for maintaining the high-amplitude rhythmicity of the peripheral clocks in a tissue-specific manner.

10.6084/m9.figshare.3145108

Cite

Citations (0)

Inflammation regulates 11β-hydroxysteroid dehydrogenase type 1 differentially in specific compartments of the gut mucosal immune system

Steroids (2017)

Peter Ergang Martin Vodička Karla Vagnerová Martin Moravec Pavlína Kvapilová

Lamina propria

Intestinal mucosa

Proinflammatory cytokine

10.1016/j.steroids.2017.07.007

Cite

Citations (4)