Zİ

Z. İpekçi

Scientific and Technological Research Council of Turkey

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Nermin Gözükırmızı

Istanbul University

Kasım Bajroviç

University of Sarajevo

Tijen Talas-Oğraş

Biotechnology Institute

Turan Tank

Istanbul University

H. Tanriyar

Pulp and Paper Research Institute

H. Balkan

Pulp and Paper Research Institute

Tahsin Akalp

Istanbul University

Kemal Kazan

Commonwealth Scientific and Industrial Research Organisation

ÖÖ

Öznur Özden

Istanbul University-Cerrahpaşa

M. Erem Çalıkoğlu

Antalya IVF

Cooperative Institutions

Istanbul University

Scientific and Technological Research Council of Turkey

Biotechnology Institute

TÜBİTAK Marmara Research Center

Marmara University

Istanbul University-Cerrahpaşa

Pulp and Paper Research Institute

Bülent Ecevit University

University of Sarajevo

Institute of Metrology of Bosnia and Herzegovina

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Genotype Differencies in Direct Plant Regeneration from Stem Explants of Populus tremula in Turkey

Journal of Forest Research (1998)

Nermin Gözükırmızı Kasım Bajroviç Z. İpekçi Melih Boydak Tahsin Akalp

Plant regeneration from 9 clones ofPopulus tremula representing diverse genotypes originated from Turkey, has been studied in different media, such as Murashige and Skoog's Medium (MS), Aspen Culture Medium (ACM) and Woody Plant Medium (WPM) with appropriate supplements of growth regulators. Regeneration efficiency on WPM containing 1 mg/l zeatin using stem explantsin vitro, was higher than on MS and ACM. In this system, the time required for shoot regeneration was 2 weeks, which was shorter by two weeks than that of previous reports. A strong genotype dependence was observed among the tested nine clones. The regeneration system, we describe here, did not involve the callus phase and thus was less prone to somaclonal variation. When adventitious microshoots were cultured on WPM supplemented with 0.5 mg/l or 2 mg/l indole-3-butyric acid (IBA), root formation was obtained after 1 week of culture. Adaptation of regenerated plantletsin vivo was 100%. This system for micropropagation ofPopulus tremula is expected to be suitable for studies on transformation of genes involved in lignin biosynthesis via modification of lignin content.

Somaclonal variation

Callus

Zeatin

Basal shoot

Plant stem

10.1007/bf02760313

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Citations (10)

Indirect somatic embryogenesis and plant regeneration from leaf and internode explants of Paulownia elongata

Plant Cell Tissue and Organ Culture (PCTOC) (2004)

Z. İpekçi Nermin Gözükırmızı

Callus

10.1007/s11240-003-4632-7

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Citations (34)

Antibacterial activity of seed proteins of Robinia pseudoacacia

Fitoterapia (2004)

Tijen Talas-Oğraş Z. İpekçi Kasım Bajroviç Nermin Gözükırmızı

Pseudomonas syringae

Robinia

10.1016/j.fitote.2004.10.020

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Citations (30)

Direct somatic embryogenesis and synthetic seed production from Paulownia elongata

Plant Cell Reports (2003)

Z. İpekçi Nermin Gözükırmızı

Kinetin

10.1007/s00299-003-0650-5

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Citations (122)

Regeneration and Transformation of populus Tremula with Antisense pOMT8 from stylosanthes Humilis

Springer eBooks (1998)

Kasım Bajroviç Z. İpekçi Tijen Talas Oğraş Nermin Gözükırmızı Melih Boydak

Stylosanthes

10.1007/978-94-011-5274-7_105

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Euphytica (2001)

Ahu Altinkut Kemal Kazan Z. İpekçi Nermin Gözükırmızı

Drought Tolerance

Drought Resistance

10.1023/a:1012067711200

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Citations (62)

Reduced leaf peroxidase activity is associated with reduced lignin content in transgenic poplar.

Plant Biotechnology (1999)

Z. İpekçi Tijen Talas Oğraş Ahu ALTLNKUT Kasım Bajroviç Kemal Kazan

A cationic peroxidase gene (Shpx6a) of a forage legume species, Stylosanthes humilis, was transferred to poplar (Populus tremula) in antisense orientation under the control of 35S RNA promoter of cauliflower mosaic virus. Transformed plants were regenerated on selective media after co-cultivation of poplar stem explants with Agrobacterium tumefaciens and integration of transgenes was confirmed by PCR and Southern hybridization analyses. Analyses of selected transgenic plants showed reductions in total leaf peroxidase activity which was 50% to 70% of that measured in untransformed control plants. Transgenic poplar plants with reduced leaf peroxidase activity had 10-20% lower lignin content than control plants. Although which isoform of poplar peroxidase (s) has been inhibited by 35S-Shpx6a antisense construct is not clearly known, our results suggested the possibility of manipulation of lignin content through inhibition of lignin-specific peroxidases.

Cauliflower mosaic virus

10.5511/plantbiotechnology.16.381

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High Frequency Somatic Embryogenesis in Cotton

Biologia Plantarum (2004)

Yildiz Aydın Z. İpekçi Tijen Talas-Oğraş Ahmet Zehir Kasım Bajroviç

A highly reproducible system for efficient somatic embryogenesis was developed to regenerate plantlets from cotton (Gossypium hirsutum L.) cultivars (Nazilli M-503 and Nazilli 143). Shoot apices, hypocotyls and nodes of 10-d-old seedlings were used as explants. High frequency (100 %) embryogenic calli was initiated from all tested explants on Murashige and Skoog (1962) (MS) media supplemented with 1 g dm-3 polyvinylpyrrolidone (PVP), 1 mg dm-3 6-benzylaminopurine (BAP), 0.5 mg dm-3 kinetin for Nazilli M-503 and 1 g dm-3 PVP, 2 mg dm-3 BAP, 0.5 mg dm-3 kinetin for Nazilli-143. Globular stage somatic embryos were produced 4 months after transfer to hormone-free MS medium supplemented with 1 g dm-3 PVP. Subsequent subculture of globular embryos every 3 weeks on hormone-free MS medium led to the development of torpedo and cotyledonary stage embryos with the frequency of 75 and 83.2 % from hypocotyl explants of Nazilli M-503 and Nazilli-143, respectively. Afterwards, mature somatic embryos were isolated and cultured on hormone-free MS medium for germination and development into plantlets. The highest germination frequency (42.9 %) for Nazilli M-503 somatic embryos were obtained on hormone-free MS medium after 5 months with hypocotyl explants, whereas germination frequencies of Nazilli-143 embryos from hypocotyl, node and apex explants varied between 22 - 30 %.

Kinetin

Subculture (biology)

Gibberellic acid

10.1023/b:biop.0000047142.07987.e1

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Citations (25)

Antisense Expression of a Caffeic AcidO-methyltransferase ofStylosanthes humilisin Transgenic Poplar: Effect of Expression onO-methyltransferase Activity and Lignin Composition

Journal of Forest Research (1999)

Kasım Bajroviç Kemal Kazan Z. İpekçi Nermin Gözükırmızı

Poplar (Populus tremula) was transformed with a construct carrying an antisense caffeic acidO-methyltransferase (COMT) cDNA (pOMT8) from a tropical pasture legume,Stylosanthes humilis. pOMT8 shows 83% overall homology to the corresponding COMT gene (pPCLA) of poplar. Of the 200 putatively-transformed plants regenerated on selective media after co-cultivation of poplar stem explants withAgrobacterium tumefaciens harbouring a CaMV 35S-antisensepOMT8 construct, a subset of 20 plants were randomly chosen for further analysis. PCR and Southern blot analysis demonstrated the stable integration of T-DNA into the genome of these plants. Antisense expression ofpOMT8 resulted in reductions in total COMT activity in the majority of the transgenic plants with the lowest total COMT activities (61–70% of untransformed control plants) being observed in four transgenic plants. The composition of lignin in transgenic plants was also changed, as detected by reductions in the content of syringyl units using infrared spectroscopy. However, no changes were found in the amount of insoluble lignin in transgenic plants as compared to untransformed control plants. These results indicate the potential of thepOMT8 gene to partially suppress COMT activity and modify the composition of lignin in transgenic poplar.

O-methyltransferase

Glufosinate

10.1007/bf02762242

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Citations (1)

HIGH FREQUENCY EMBRYOGENIC CALLUS INDUCTION, SOMATIC EMBRYO FORMATION AND PLANT REGENERATION FROM DIFFERENT EXPLANTS OF COTTON

Yildiz Aydın Z. İpekçi Tijen Talas-Oğraş Kasım Bajroviç A. Zchir

Callus

10.5072/zenodo.13014

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Citations (0)