D.M. Strong

University of Washington

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W. A. Blattner

National Cancer Institute

K W Sell

Nova Southeastern University

Peter Ebbesen

Aalborg University

G.D. Bonnard

National Institutes of Health

Robert J. Biggar

National Institutes of Health

Franco Pandolfi

Università Cattolica del Sacro Cuore

Mads Melbye

Danish Cancer Society

M Popovic

University of Maryland, Baltimore

A E Maluish

National Cancer Institute

JamesJ. Goedert

National Cancer Institute

Cooperative Institutions

National Institutes of Health

National Cancer Institute

University of Maryland, Baltimore

Pham Ngoc Thach University of Medicine

Naval Medical Research Command

Biomedical Research Institute

Johns Hopkins University

Uniformed Services University of the Health Sciences

Cancer Institute (WIA)

Georgetown University

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Seminal fluid excretion of cytomegalovirus related to immunosuppression in homosexual men.

BMJ (1983)

Robert J. Biggar H K Andersen Peter Ebbesen Mads Melbye JamesJ. Goedert

Seminal fluid samples from 84 Danish homosexual men were successfully cultured to determine the prevalence of cytomegalovirus excretion. Ten (15%) out of 66 men positive for the antibody were found to be excreting the virus. Although the proportion excreting was inversely related to age (p less than 0.01), three men aged over 30 and with many years of homosexual experience excreted the virus. In addition, a 50 year old man with Kaposi9s sarcoma excreted the virus. A further study of the ratio of T cell helpers to suppressors in the men aged over 30 and a series of age matched non-excreting homosexual control or heterosexual men showed that those excreting cytomegalovirus in their seminal fluid had statistically lower ratios (all less than 0.77) than the controls (p less than 0.05). Excretion of cytomegalovirus may be related to re-emergence of latent infection in immunosuppressed homosexual men.

Cytomegalovirus

Immunosuppression

10.1136/bmj.286.6383.2010

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Differential recovery of K and NK cell function following cryopreservation of human mononuclear cells

Cryobiology (1981)

D.M. Strong JR Ortaldo Franco Pandolfi A E Maluish R B Herberman

Cell function

10.1016/0011-2240(81)90173-5

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Cell surface antigen expression in newborn cord blood lymphocytes infected with HTLV.

The Journal of Immunology (1983)

Douglas L. Mann M Popovic C Murray Carolyn Y. Neuland D.M. Strong

Human T cell lymphocyte lines, established from lymphoid tissues from patients with adult T cell malignancies infected with the human T cell lymphoma virus (HTLV), were used in co-culture experiments to infect newborn cord blood lymphocytes (CBL). The infected and non-infected CBL cell lines were typed for HLA alloantigen determinants and tested for cell surface antigens using selected monoclonal antibodies. Infected cord blood lymphocytes showed inappropriate expression of alloantigenic determinants of the HLA-A and -B alleles. The monoclonal antibody 4D12, detecting an antigen common to the HLA-B5 cross-reactive group, was reactive with all infected cultures; this determinant appeared de novo in CBL cells lacking B5 cross-reactive group antigens in the uninfected state, and it increased in density in the infected cultures where the HLA-B5 cross-reactive alloantigens were present in uninfected CBL. HLA-DR was expressed in low levels or not detected on non-infected cultured cord blood lymphocytes and was present on all infected cells. OKT4 positive cells predominated in infected cultures, whereas OKT8 positive cells decreased in number or were absent. The TCGF receptor also increased both in density and in percent positive cells in all infected cell lines. The results suggest that HTLV may be tropic for a subset of T cells expressing mature T cell markers and that viral infections directly affect expression of cell surface antigens controlled by the human major histocompatibility complex (MHC).

Cord blood

10.4049/jimmunol.131.4.2021

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Citations (35)

Tritiated thymidine incorporation and cell-mediated lympholysis as correlates of acute graft-versus-host reaction.

PubMed (1977)

Saul J. Sharkis D.M. Strong Aftab Ahmed K W Sell

Thymidine

Histocompatibility

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CRYOPRESERVATION OF ORGANS AND TISSUE

WORLD SCIENTIFIC eBooks (1998)

G O Phillips D.M. Strong Rüdiger von Versen Aziz Nather

10.1142/9789812816078_others04

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Identification of the human T cell lymphoma virus in B cell lines established from patients with adult T cell leukemia.

Journal of Clinical Investigation (1984)

Dean L. Mann Jeffrey W. Clark Michael F. Clarke M S Reitz M Popovic

Cell lines were established from the peripheral blood of two patients with adult T cell leukemia. In contrast to our previous experience, where all such lines expressed T cell markers, these two cell lines expressed B cell antigens and Ig light chains (kappa on CF-2, lambda on HS). Human T cell lymphoma proviral (HTLV) sequences were demonstrated in both cell lines. Since only a portion of the cells in culture expressed Ig light chains, experiments were carried out to exclude the possibility that the cultures were not a mixture of B and T or non-B cells. Cells that expressed kappa- or lambda-light chains were separated by cell sorting from kappa- or lambda-negative cells and replaced in culture. Light chain negative cells reexpressed light chains after time in culture. After 5-azacytidine treatment of the cell lines, all cells expressed Ig light chains. These studies show that the human retrovirus HTLV, which has been demonstrated to be associated with certain T cell malignancies, can infect B cells or B cell precursors.

10.1172/jci111418

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Citations (35)

AMYL NITRITE MAY ALTER T LYMPHOCYTES IN HOMOSEXUAL MEN

The Lancet (1982)

JamesJ. Goedert WilliamC. Wallen DeanL. Mann D.M. Strong CarolynY. Neuland

Amyl nitrite

10.1016/s0140-6736(82)91617-8

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Citations (169)

Cryopreservation of human cultured T cells for use as responder cells in an tnterleukin-2 determination system

Cryobiology (1981)