Bernard Botton

Université de Lorraine

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Michel Chalot

Université de franche-comté

Annick Brun

Université de Lorraine

Daniel Wipf

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Damien Blaudez

Université de Lorraine

J. J. Guillaumin

Génétique, Diversité, Écophysiologie des Céréales

Pierre Dizengremel

Université de Lorraine

Arnaud Javelle

University of Strathclyde

François Le Tacon

Interactions Arbres-Microorganismes

Francis Martin

Interactions Arbres-Microorganismes

B. Dell

Murdoch University

Cooperative Institutions

Université de Lorraine

Institut National de la Recherche Agronomique

Interactions Arbres-Microorganismes

Centre National de la Recherche Scientifique

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Laboratoire de Chimie Physique et Microbiologie pour l'Environnement

Ecologie et Ecophysiologie Forestières

Laboratoire Physiologie Cellulaire & Végétale

Centre National de la Propriété Forestière

Jagiellonian University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Influence of calcium on the differentiation and growth of aggregated organs in Sphaerostilbe repens

Canadian Journal of Microbiology (1978)

Bernard Botton

In the ascomycete Sphaerostilbe repens the vegetative mycelium aggregated into structures composed of aerial coremia and rhizomorphs immersed in the culture medium. The morphology and growth of the cultures were examined on a defined medium with and without calcium supplementation.Initiation and growth of aggregated organs occurred only if calcium was present continuously in the culture medium. On calcium-deficient media the fungus produced only vegetative mycelium which retained its ability to aggregate in the presence of the cation. Small concentrations of calcium (less than 1 ppm) stimulated aggregation of the mycelium, but higher concentrations (about 200 ppm) were necessary to obtain optimum development of the aggregated organs. Strontium partially replaced calcium, but lithium did not, as a stimulator of aggregation.

Repens

Chemically defined medium

10.1139/m78-172

Cite

Citations (10)

Molecular characterization, function and regulation of ammonium transporters (Amt) and ammonium‐metabolizing enzymes (GS, NADP‐GDH) in the ectomycorrhizal fungus Hebeloma cylindrosporum

Molecular Microbiology (2003)

Arnaud Javelle Mélanie Morel‐Rouhier Blanca‐Rosa Rodríguez‐Pastrana Bernard Botton Bruno André

Summary External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structures of the ectomycorrhizal symbiosis. Here, we have cloned and characterized Hebeloma cylindrosporum AMT1 , GLNA and GDHA genes, which encode a third ammonium transporter, a glutamine synthetase and an NADP‐dependent glutamate dehydrogenase respectively. Amt1 can fully restore the pseudohyphal growth defect of a Saccharomyces cerevisiae mep2 mutant, and this is the first evidence that a heterologous member of the Mep/Amt family complements this dimorphic change defect. Dixon plots of the inhibition of methylamine uptake by ammonium indicate that Amt1 has a much higher affinity than the two previously characterized members (Amt2 and Amt3) of the Amt/Mep family in H. cylindrosporum . We also identified the intracellular nitrogen pool(s) responsible for the modulation of expression of AMT1 , AMT2 , AMT3 , GDHA and GLNA . In response to exogenously supplied ammonium or glutamine, AMT1 , AMT2 and GDHA were downregulated and, therefore, these genes are subjected to nitrogen repression in H. cylindrosporum . Exogenously supplied nitrate failed to induce a downregulation of the five mRNAs after transfer of mycelia from a N‐starved condition. Our results demonstrate that glutamine is the main effector for AMT1 and AMT2 repression, whereas GDHA repression is controlled by intracellular ammonium, independently of the intracellular glutamine or glutamate concentration. Ammonium transport activity may be controlled by intracellular NH 4 + . AMT3 and GLNA are highly expressed but not highly regulated. A model for ammonium assimilation in H. cylindrosporum is presented.

10.1046/j.1365-2958.2003.03303.x

Cite

Citations (119)

Purification and characterization of an anti-(A+B) specific lectin from the mushroom Hygrophorus hypothejus

Biochimica et Biophysica Acta (BBA) - General Subjects (1999)

Bertrand Veau Jacques Guillot Mireille Damez Martine Dusser Grażyna Końska

Molecular mass

10.1016/s0304-4165(99)00055-0

Cite

Citations (17)

Immunological properties of a lectin isolated from the phytopathogenic basidiomycete Rigidoporus lignosus [Ouchterlony double diffusion]

Tristan Richard Jacques Guillot Bernard Botton

Ouchterlony double immunodiffusion

Source

Cite

Citations (0)

An update on nutrient transport processes in ectomycorrhizas

Springer eBooks (2002)

Michel Chalot Arnaud Javelle Damien Blaudez Raphaël Lambilliote Richard Cooke

Nutrient Sensing

Mycorrhizal Fungi

10.1007/978-94-017-1284-2_16

Cite

Citations (79)

Seasonal fluctuations of growth and nitrogen metabolism in the ectomycorrhizal association Douglas-fir/Laccaria laccata

HAL (Le Centre pour la Communication Scientifique Directe) (1989)

Michel Chalot Bernard Botton Jacques Banvoy

Douglas fir

Ectomycorrhiza

Nitrogen Cycle

Source

Cite

Citations (0)

Variation in the ITS and IGS regions of ribosomal DNA among the biological species of European Armillaria

Mycological Research (1998)

Mohammed Chillali Hakima Idder-Ighili J. J. Guillaumin CL Mohammed B. Lung Escarmant

Armillaria

Spacer DNA

Intergenic region

10.1017/s0953756297005315

Cite

Citations (78)

The aspartate aminotransferase of the ectomycorrhizal fungus Cenococcum geophilum : purification and molecular properties

HAL (Le Centre pour la Communication Scientifique Directe) (1988)

Ahmed Khalid A. Boukroute Bernard Botton Francis Martin

Source

Cite

Citations (13)

Glutamate dehydrogenases in ectomycorrhizas of spruce (Picea excelsa L. and beech (Fagus sylvatica L.)

HAL (Le Centre pour la Communication Scientifique Directe) (1989)

B. Dell Bernard Botton F. Martin François Le Tacon

Source

Cite

Citations (0)

Purification and Characterization of Glutamine Synthetase and NADP-Glutamate Dehydrogenase from the Ectomycorrhizal Fungus Laccaria laccata

PLANT PHYSIOLOGY (1992)

Annick Brun Michel Chalot Bernard Botton Francis L. Martin

Glutamine synthetase (GS) and NADP-dependent glutamate dehydrogenase (NADP-GDH) play a key role in nitrogen assimilation in the ectomycorrhizal fungus Laccaria laccata (Scop. ex Fr. Cke) strain S 238. The two enzymes were purified to apparent electrophoretic homogeneity by a three-step procedure involving diethylaminoethyl (DEAE)-Trisacryl and affinity chromatography, and DEAE-5PW fast protein liquid chromatography. This purification scheme resulted in a 23 and 62% recovery of the initial activity for GS and NADP-GDH, respectively. Purified GS had a specific activity of 713 nanomoles per second per milligram protein and a pH optimum of 7.2. Michaelis constants (millimolar) for the substrates were NH(4) (+) (0.024), glutamate (3.2), glutamine (30), ATP (0.18), and ADP (0.002). The molecular weight (M(r)) of native GS was approximately 380,000; it was composed of eight identical subunits of M(r) 42,000. Purified NADP-GDH had a specific activity of 4130 nanomoles per second per milligram protein and a pH optimum of 7.2 (amination reaction). Michaelis constants (millimolar) for the substrates were NH(4) (+) (5), 2-oxoglutarate (1), glutamate (26), NADPH (0.01), and NADP (0.03). Native NADP-GDH was a hexamer with a M(r) of about 298,000 composed of identical subunits with M(r) 47,000. Polyclonal antibodies were produced against purified GS and NADP-GDH. Immunoprecipitation tests and immunoblot analysis showed the high reactivity and specificity of the immune sera against the purified enzymes.

Glutamate synthase

Isocitrate dehydrogenase

Polyclonal antibodies

10.1104/pp.99.3.938

Cite

Citations (54)