Manju Modgil

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

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Manisha Thakur

Jamia Hamdard

Arjun Chauhan

GLA University

Deepa Sharma

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

S. V. Bhardwaj

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

J. N. Sharma

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

Manchikatla Venkat Rajam

University of Delhi

S. K. Chakrabarti

Mahatma Gandhi Memorial Medical College

Kishore Khosla

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

Nitisha Negi

Kumaun University

R. Handa

Cooperative Institutions

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

University of Delhi

Indian Council of Agricultural Research

Punjab Agricultural University

Jawaharlal Nehru Krishi Vishwa Vidyalaya

Indian Agricultural Research Institute

Swami Keshwanand Rajasthan Agricultural University

University of Rajasthan

Landscape Institute

North Bengal Agricultural University

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Development of high frequency in vitro shoot regeneration system from leaves of apple cultivar ‘Oregon Spur’ and optimization of antibiotics concentration

Indian Journal of Experimental Biology (2023)

Neha Kumari Manju Modgil

Optimization of efficient shoot regeneration protocol and selection of transformed plants at optimum level of antibioticsare pre-requisite in genetic engineering. Here, we explored standardization of in vitro shoot regeneration protocol from leafexplants of apple cv. Oregon Spur for the first time using different concentrations of plant growth regulators. Further, wedetermined the effect of two antibiotics (kanamycin and cefotaxime) on shoot regeneration efficiency. About 95% of leavesinduced adventitious shoots on MS medium supplemented with 4 mg/L BA and 0.2 mg/L NAA. The frequency of shootregeneration increased to 100% when leaf explants were cultured on 2 mg/L TDZ along with 0.5 mg/L NAA. The number ofshoots regenerated per leaf was enhanced but abnormal and vitrified shoots were obtained in case of TDZ supplementedmedium. Dark incubation of initial one week decreased the shoot regeneration frequencies at all the combinations of plantgrowth regulators. Leaf explants were found highly sensitive to kanamycin and inhibited shoot induction above 5 mg/L.This concentration was found suitable for selection of transgenic shoots. Cefotaxime at 100-300 mg/L promotedorganogenesis, whereas 500 mg/L was found optimal to eliminate Agrobacterial overgrowth after co-cultivation with leafexplants. Our results have demonstrated that the above protocol can successfully be applied for transgenic development in‘Oregon Spur’ cultivar of apple in future trials.

Kanamycin

10.56042/ijeb.v61i06.1945

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Citations (1)

In vitro screening of apple rootstock MM106 somaclones with Phytophthora cactorum culture filtrate

Journal of Plant Pathology (2021)

Shalini Verma Manju Modgil Shirish Patidar

Phytophthora cactorum

Browning

10.1007/s42161-020-00722-z

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A reproducible protocol for adventitious shoot regeneration from leaves of apple rootstock Merton793 and assessment of genetic stability

Indian Journal of Horticulture (2020)

Malti Sharma Manju Modgil

The present studies were aimed to develop an efficient and reproducible method for shoot regenerationfrom leaf explants of apple rootstock Merton793. The influence of two cytokinins BA and TDZ with three auxinsIAA, IBA and NAA in different combinations were evaluated for shoot induction using light and dark incubation.Direct and healthy shoots were obtained from leaves on MS medium with BA-NAA and BA-IAA combinations inlight incubation and 100% regeneration was achieved on medium with 4 mg/l BA and 1 mg/l NAA. Both directand indirect shoot regeneration was obtained on TDZ-NAA and TDZ-IBA. Maximum shoot regeneration waspromoted on 0.8 & 0.6 mg/l TDZ with 1 mg/l NAA, however, it showed abnormal, vitrified, small and rosettetype of shoots. Dark treated leaf explants showed low rates of shoot induction with TDZ combinations. Healthyadventitious shoots regenerated directly from leaves were multiplied, rooted and hardened successfully. RAPD analysis of regenerants revealed that all the banding profiles from directly originated shoots were monomorphicand similar to those of the mother plant, while shoots originated through intervening callus revealed 61.2%polymorphism. The results show that genetic fidelity of directly produced adventitious shoots was maintained,and thus, our regeneration protocol can be an useful method in transformation research in Merton793 withoutthe induction of variation.

Callus

Basal shoot

10.5958/0974-0112.2020.00060.2

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Successful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol.

PubMed (2000)

Meenakshi Sharma Manju Modgil Deepa Sharma

Successful in vitro propagation of clonal apple rootstock MM106 was achieved by culturing axillary buds on MS basal medium with BAP (1 mg/L), GA3 (0.5 mg/L) and IBA (0.1 mg/L). Use of liquid medium (LM) in initial cultures reduced phenol exudation to a greater extent and gave maximum sprouting percentage when transferred to solid MS medium. Phloroglucinol (PG) did not enhance sprouting of buds but increased the rate of multiplication when added in the medium. Maximum number of shoots were obtained when MS medium was supplemented with BAP (0.5 mg/L), GA3 (1 mg/L), IBA (0.1 mg/L) and PG (100 mg/L). For rooting, in vitro regenerated shoots were placed in IBA (30 mg/L) for 3 hr and transferred to solidified auxin free medium. Rooting was recorded in about 80% of shoots. Inclusion of PG in rooting medium was not beneficial but shoot cultures grown in its presence gave higher rooting percentage. Rooted plantlets showed about 70% survival rate in potting mixture of sand:soil:perlite (1:1:1).

Sprouting

Phloroglucinol

Vermiculite

Perlite

Potting

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Studies on micropropagation of gerbera (Gerbera jamesonii Bolus)

Indian Journal Of Applied Research (2011)

Bhavya Bhargava BS Dilta Y.C. Gupta S. R. Dhiman Manju Modgil

Gerbera jamesonii

10.15373/2249555x/nov2013/4

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Citations (7)

STUDIES ON INDUCTION OF ROOTING IN IN VITRO GROWN SHOOTS OF APPLE CLONAL ROOTSTOCK MERTON 793

Agrotechnology (2009)

Manju Modgil Nitisha Negi

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Screening of Indian wild apple accessions with scab and powdery mildew disease resistant gene specific molecular markers

International Journal of Farm Sciences (2022)

Vikrant Gautam Manju Modgil

Investigations on resistance to pathogens within local wild populations is the most efficient way for developing resistant cultivars in breeding programmes. In this study, Indian wild crab apple (Malus baccata Borkh var Himalaica) accessions maintained at two field gene banks in Himachal Pradesh were screened to find out the resistant sources. Individual trees were tested for the presence/absence of Vf, Vrl and Vbj scab resistant and Pl1 and Pl2 mildew resistant genes with eight and one SCAR markers respectively tightly linked to these genes. The potted plants were evaluated after the inoculation of pathogen. All the accessions showed diversity in relation to resistant gene to both the pathogens. All the SCAR markers K08, T06, Al07, Am19, ACS07, ACS09, Z13 and S22 were present in eight crab apple accessions. PLBJ SCAR marker linked with major genes Pl1 and Pl2 was detected in six accessions. Molecular marker assessment results almost corresponded to the data received in pot assay experiments thus were in good agreement. The study confirmed that these accessions carry scab and mildew resistant genes which could play important role in selection of desirable genotypes for apple breeding as well as use as suitable rootstock.

10.5958/2250-0499.2022.00098.2

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Citations (1)

Genetic variability and correlation in biochemical parameters in Rubus ellipticus Sm.

Indian Journal of Horticulture (1994)

Manju Modgil D. Modgil

Rubus

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Impact of cefotaxime and kanamycin on in vitro regeneration via Agrobacterium mediated transformation in apple cv. Red Chief

Plant Physiology Reports (2023)