Takashi Yokochi

Toyota Motor Corporation (Japan)

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Localization of Apoptosis (Programmed Cell Death) in Mice by Administration of Lipopolysaccharide

Microbiology and Immunology (1994)

Xian‐min Zhang Akiko Morikawa Kazuko Takahashi Guozhi Jiang Yutaka Kato

Abstract Localization of apoptotic cells by administration of lipopolysaccharide into mice was studied by using the in situ specific labeling of fragmented DNA. This method clearly stained the nuclei of thymocytes at the cortex of the thymus. The nuclei of cells in the bone marrow and in the spleen were also positively stained. It was suggested that the cortex in the thymus is where the LPS‐induced programmed cell death occurs.

10.1111/j.1348-0421.1994.tb01838.x

Cite

Citations (42)

The Inhibitory Action of Sodium Arsenite on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 267.4 Macrophage Cells: A Role of Raf-1 in Lipopolysaccharide Signaling

The Journal of Immunology (2001)

Dipshikha Chakravortty Yutaka Kato Tsuyoshi Sugiyama Naoki Koide Mya Mya Mu

Abstract The effect of sodium arsenite (SA) on LPS-induced NO production in RAW 267.4 murine macrophage cells was studied. SA pretreatment of LPS-stimulated RAW cells resulted in a striking reduction in NO production. No significant difference in LPS binding was observed between RAW cells pretreated with SA and control untreated RAW cells, suggesting that SA might impair the intracellular signal pathway for NO production. SA inhibited LPS-induced NF-κB activation by preventing loss of IκB-α and -β. Furthermore, SA blocked phosphorylation of extracellular signal-regulated kinase 1/2 (Erk1/2), but not phosphorylation of p38 and c-Jun N-terminal kinase. SA treatment resulted in the disappearance of Raf-1, suggesting that it might cause the inhibition of the Erk1/2 mitogen-activated protein (MAP) kinase pathway. The SA-mediated loss of Raf-1 also abolished LPS-induced NF-κB activation as well as the Erk1/2 pathway. The dominant negative mutant of MAP kinase kinase 1 inhibited both NO production and NF-κB activation in LPS-stimulated RAW cells. Taken together, these results indicate that the inhibitory action of SA on NO production in LPS-stimulated macrophages might be due to abrogation of inducible NO synthase induction, and it might be closely related to inactivation of the NF-κB and Erk1/2 MAP kinase pathways through loss of Raf-1.

Sodium arsenite

10.4049/jimmunol.166.3.2011

Cite

Citations (57)

Role of tumor necrosis factor-α and glucocorticoid on lipopolysaccharide (LPS)-induced apoptosis of thymocytes

FEMS Immunology & Medical Microbiology (1995)

Yoshiro Kato Akiko Morikawa Tsuyoshi Sugiyama Naoki Koide G-Z. Jiang

Administration of bacterial lipopolysaccharide (LPS) into mice markedly induced the apoptosis of CD4+8+ thymocytes. The injection of anti-tumor necrosis factor (TNF)-alpha antibody or RU38486, a glucocorticoid receptor antagonist, into mice definitely inhibited LPS-induced apoptosis of thymocytes. Addition of the sera 1 h after injection of LPS into in vitro cultures of thymocytes caused thymocyte apoptosis. It was also prevented by either anti-TNF-alpha antibody or RU38486. Further, recombinant TNF-alpha and hydrocortisone collaborated in induction of the thymocyte apoptosis in vitro. The in vivo phenomenon of LPS-induced apoptosis of thymocytes was reproducible by the in vitro experimental system. It was therefore suggested that both TNF-alpha and glucocorticoid participate and collaborate as effector molecules in LPS-induced apoptosis of thymocytes.

Thymocyte

10.1111/j.1574-695x.1995.tb00192.x

Cite

Citations (52)

Butyrate enhances the production of nitric oxide in mouse vascular endothelial cells in response to gamma interferon

Journal of Endotoxin Research (2004)

Akiko Morikawa Tsuyoshi Sugiyama Naoki Koide Isamu Mori Mya Mya Mu

The effect of butyrate, a natural bacterial product of colonic bacterial flora, on nitric oxide (NO) production in murine vascular endothelial cell line END-D in response to IFN-γ and/or LPS was studied. Butyrate significantly augmented NO production in END-D cells in response to IFN-γ or IFN-γ + LPS, but not LPS alone. The NO production was augmented by the addition of butyrate until 6 h after the stimulation with IFN-γ or IFN-γ + LPS. The augmentation was abolished by the removal of butyrate from the cultures. Butyrate enhanced the expression of inducible type NO synthase (iNOS) in the stimulated END-D cells. Furthermore, butyrate-enhanced NO production in the presence of various signal inhibitors down-regulating the signal pathways using nuclear factor (NF)-κB, mitogen-activated protein (MAP) kinases and Janus tyrosine kinase. The putative mechanism of butyrate-induced augmentation of NO production in response to IFN-γ or IFN-γ + LPS is discussed.

10.1177/09680519040100010401

Cite

Citations (15)

THERAPEUTIC POTENTIAL OF ENDOTOXIN-NEUTRALIZING PROTEINS FOR SEPSIS AND ENDOTOXIN SHOCK

Shock (1995)

Hiraia Michimasa Takashi Yokochi Wright Susan C Larrick James W

Michimasa, Hiraia*; Takashi, Yokochi**; C, Wright Susan***; W, Larrick James*** Author Information

10.1097/00024382-199512001-00019

Cite

Citations (0)

Strong activation of complement system by lipopolysaccharides (LPS) possessing mannose homopolysaccharides.

PubMed (1990)

Takashi Yokochi

Complement

Source

Cite

Citations (0)

Induction of human leukocyte antigen-A,B,C and -DR on cultured human oligodendrocytes and astrocytes by human γ-interferon

Neuroscience Letters (1986)

Masaaki Hirayama Takashi Yokochi Kaoru Shimokata Mitsuo Iida Norio Fujiki

Neuroglia

10.1016/0304-3940(86)90543-4

Cite

Citations (30)

Combined semi-nested polymerase chain reaction and heteroduplex analysis for detecting monoclonality of IgH rearrangement in patients with follicular lymphoma.

PubMed (2004)

Amaylia Oehadian Naoki Koide Mya Mya Mu Ferdaus Hassan Tomoaki Yoshida

A new, sensitive method combining seminested polymerase chain reaction (PCR) and heteroduplex analysis was used to detect follicular lymphoma (FL) cells in peripheral blood. Based on the detection of IgH rearrangement in DNA from peripheral blood leukocytes, the method demonstrated the presence of monoclonal B cells in FL patients with high frequency.

Heteroduplex

Follicular lymphoma

Gene rearrangement

Source

Cite

Citations (0)

The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS

Journal of Endotoxin Research (2001)

Tsuyoshi Sugiyama Naoki Koide Dipshikha Chakravortty Yutaka Kato Mya Mya Mu

The surface expression of CD14 on mouse B-1 cells and its role on their response to lipopolysaccharide (LPS) were studied by using the murine TH2.52 B-1 cell line and peritoneal B-1 cells. TH2.52 cells with the B-1 phenotype were found to express membrane-bound CD14. Furthermore, CD14 was expressed on physiological peritoneal CD5 + B-1 cells. The stimulation of CD14-expressing TH2.52 cells with a low concentration of LPS resulted in the activation of nuclear factor (NF)-κB and a mitogen-activated protein kinase (MAPK). The LPS-induced NF-κB and MAPK activation was markedly inhibited by anti-CD14 antibody. These results suggest that B-1 cells may respond to LPS via membrane-bound CD14.

10.1177/09680519010070030501

Cite

Citations (5)

Establishment of hybridoma cells with natural killer(NK)-like activity against syngenic tumor cells.

PubMed (1992)

Yasumoto Suzuki Mitsuru Funabashi Shin Isomura Kazuhiro Yoshikawa Kunihiro Notake

The 4D1D4 hybridoma cells were derived from the fusion of spleen cells from BALB/c nude mice with NS-1 mouse myeloma cells. The surface phenotypes of 4D1D4 hybridoma cells were Thy-1.2+, L3T4 (CD4)-, Lyt-2 (CD8)-, Asialo GM1+ and p-55 interleukin-2 (IL-2) receptor (CD25)-. This phenotypic pattern was consistent with the surface phenotype of NK cells. The 4D1D4 cells showed the definite killer activity against a syngenic tumor cell line, RL male-1, but not against an allogenic YAC-1 line. The killer activity of the 4D1D4 cells was not affected by the addition of exogenous IL-2. It was, therefore, suggested that 4D1D4 cells might be representative of resting NK cells with expression of no functional IL-2 receptors. The hybridoma technology might be useful for establishment of the cloned NK cells.

Syngenic

Lymphokine-activated killer cell

Interleukin 3

Interleukin 15

Source

Cite

Citations (2)