Carlos F. Amábile-Cuevas

Mexfam

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Irma Rosas

Universidad Nacional Autónoma de México

José Luis Arredondo-Garcı́a

Instituto Nacional de Pediatria

Ana Margarita Obregón Fuentes

Hospital Universitario Reina Sofía

Leticia Martínez

Hospital Maciel

Gerardo Hernández-Oliva

Bayer (Mexico)

Marina Chicurel

Santa Cruz County Office of Education

Eva Salinas

Autonomous University of Aguascalientes

Edmundo Calva

Universidad Nacional Autónoma de México

Chii Shang

University of Hong Kong

Oriana Jovanovic

Delft University of Technology

Cooperative Institutions

Universidad Nacional Autónoma de México

Harvard University

Centro Médico Nacional La Raza

Mexican Social Security Institute

University of Canterbury

Bayer (Germany)

Hospital Infantil de México Federico Gómez

Instituto Nacional de Salud Pública

Pontificia Universidad Católica de Chile

Universidad Autónoma de la Ciudad de México

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Metallo-beta-lactamase-producing Escherichia coli in the sewage of Mexico City: where do they come from?

Canadian Journal of Microbiology (2021)

Kathia Lüneberg Carlos F. Amábile-Cuevas Eduardo Mucito-Varela Leticia Martínez Eva Salinas

While monitoring the presence of antibiotic resistance in municipal wastewater bacteria from Mexico City, five Escherichia coli isolates were found to be resistant to carbapenems, antibiotics of "last resort" used mostly in hospitals. Further analysis revealed that these carbapenem-resistant isolates carried the gene encoding a metallo-beta-lactamase, NDM-5. The gene was found to be beared by a large, ∼145 kb conjugative plasmid, which also carries putative genes encoding resistance to sulfonamides, trimethoprim, tetracycline, ciprofloxacin, and chloramphenicol (although no phenotypic chloramphenicol resistance was detected) and quaternary-ammonium compounds. The plasmid also carried gene mobility determinants, such as integron integrase and two transposases. In addition to the direct public health threat posed by the presence of such multi-resistant organisms in wastewater released into the environment and used for crop irrigation; it is particularly concerning that carbapenem-resistant E. coli is rather rare in Mexican hospitals (<1%), but was found in small, 100 mL samples of municipal wastewater. This suggests that these organisms are under-reported by clinical microbiology laboratories, underlining the usefulness of wastewater monitoring, or that there is an unknown source of such carbapenem-resistant organisms that are being dumped into the wastewater. The source of these bacteria must be assessed and controlled to prevent further spread of this multi-resistance plasmid among other environmental and clinical microorganisms.

Acinetobacter baumannii

10.1139/cjm-2021-0284

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Citations (2)

Horizontal Gene Transfer

Springer eBooks (2011)

Carlos F. Amábile-Cuevas Marina Chicurel

Horizontal Gene Transfer

Gene transfer

10.1007/978-3-642-16483-5_2808

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Citations (26)

Ascorbate and Antibiotics, at Concentrations Attainable in Urine, Can Inhibit the Growth of Resistant Strains of Escherichia coli Cultured in Synthetic Human Urine

Antibiotics (2023)

Carlos F. Amábile-Cuevas

There are conflicting reports on the antibacterial activity of ascorbate; all at concentrations much higher than the typical in human plasma, but that can be reached in urine. The effect of 10 mM ascorbate (in itself not inhibitory) along with antibiotics, was tested both in Mueller-Hinton broth (MHb) and in synthetic human urine (SHU), against resistant isolates of Escherichia coli from lower urinary infections. The activity of nitrofurantoin and sulfamethoxazole was higher in SHU than in MHb; minimal inhibitory concentrations (MICs) in SHU with ascorbate were below typical urinary concentrations. For other antibiotics, MICs were the same in MHb vs. SHU, with no effect of ascorbate in MHb; but in SHU with ascorbate, MICs of ciprofloxacin and gentamicin also went below reported urinary concentrations, with a lesser effect with norfloxacin and trimethoprim, and none with ampicillin. The effect of ascorbate was independent of oxygen and not related to the susceptibility of each strain to oxidative stress. Ascorbate oxidizes during incubation in SHU, and bacterial growth partially prevented oxidation. These results suggest that 10 mM ascorbate can enhance the inhibitory activity of antibiotics upon resistant strains in urine. Clinical experimentation with ascorbate-antibiotic combinations against urinary infections caused by resistant bacteria is warranted.

Trimethoprim

Nitrofurantoin

10.3390/antibiotics12060985

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Citations (3)

Fluoroquinolone resistance in clinical and environmental isolates of Escherichia coli in Mexico City

Journal of Applied Microbiology (2009)

Carlos F. Amábile-Cuevas J.L. Arredondo-GarcÃa A. Cruz Irma Rosas

To assess the different phenotypes and mechanisms of fluoroquinolone (FQ) resistance in clinical and environmental isolates of Escherichia coli.We compared FQ-resistant E. coli isolates, measuring minimal inhibitory concentrations (MIC) of ciprofloxacin, along with susceptibility to other antibiotics. We also searched for the presence of efflux pumps, using efflux inhibitors, and for plasmid-borne FQ-resistance by PCR. We found that, aside from the higher FQ-resistance prevalence among clinical strains, environmental ones resist much lower concentrations of ciprofloxacin. Efflux pumps mediate fluoroquinolone resistance as frequently among environmental isolates than in clinical strains. Plasmid-borne qnrA genes were not detected in any resistant strain.Environmental FQ-resistant strains may have a nonclinical origin and/or a selective pressure different from the clinical use of FQs.The identification of the source of low-level FQ-resistant strains (ciprofloxacin MIC c. 8 microg ml(-1)) in the environment could be important to curb the rapid emergence and spread of FQ-resistance in clinical settings, as these strains can easily become fully resistant to FQ concentrations achievable in fluids and tissues during therapy.

10.1111/j.1365-2672.2009.04401.x

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Citations (36)

Moxifloxacin vs amoxicillin/clavulanate in the treatment of acute sinusitis

American Journal of Otolaryngology (2007)

José R. Arrieta Alejandro S. Galgano Eulália Sakano Ximena Fonseca Carlos F. Amábile-Cuevas

Discontinuation

10.1016/j.amjoto.2006.06.016

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Citations (22)

What Water Professionals Should Know about Antibiotics and Antibiotic Resistance: An Overview

ACS ES&T Water (2021)

Oriana Jovanovic Carlos F. Amábile-Cuevas Chii Shang Chao Wang K W Ngai

In light of recent predictions that by 2050, antimicrobial resistance will cause almost 10 million deaths annually, costing the global economy 100 trillion U.S. dollars, the examination of antibiotic resistance on both local and global scales has become essential. Antibiotic resistance is a multidimensional problem that connects water professionals with many scientists from different professions. In this review, we provide the big picture to the researchers venturing into this topic with respect to wastewater treatment and water safety, focusing on two aspects: antibiotics and antibiotic resistance and how they are intertwined. The story opens with the history of antibiotics, followed by their classes, modes of action and main bacterial targets, uses in clinical settings and agriculture, and presence in wastewater and the environment. The section about antibiotic resistance starts with its definition in clinical and environmental settings, followed by types and means of genetic transfer, prioritization, environmental risk and the current regulation framework, and the fate of antibiotic resistant bacteria and their genes throughout the wastewater treatment plant. The review ends with recommendations that should help water professionals in making educated decisions in research pursuits and avoiding common pitfalls when embarking on a journey of antibiotic resistance.

Prioritization

10.1021/acsestwater.0c00308

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Citations (45)

Characterization of Escherichia coli Isolates from an Urban Lake Receiving Water from a Wastewater Treatment Plant in Mexico City: Fecal Pollution and Antibiotic Resistance

Current Microbiology (2015)

Irma Rosas Eva Salinas Leticia Martínez Ariadnna Cruz‐Córdova Bertha González‐Pedrajo

Indicator bacteria

10.1007/s00284-015-0877-8

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Citations (35)

Bacterial plasmids and gene flux

Cell (1992)

Carlos F. Amábile-Cuevas Marina Chicurel

10.1016/0092-8674(92)90095-t

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Citations (142)

Resistance to Antibiotics of Clinical Relevance in the Fecal Microbiota of Mexican Wildlife

PLoS ONE (2014)

Jurgi Cristóbal‐Azkarate Jacob C. Dunn Jennifer M. W. Day Carlos F. Amábile-Cuevas

There are a growing number of reports of antibiotic resistance (ATBR) in bacteria living in wildlife. This is a cause for concern as ATBR in wildlife represents a potential public health threat. However, little is known about the factors that might determine the presence, abundance and dispersion of ATBR bacteria in wildlife. Here, we used culture and molecular methods to assess ATBR in bacteria in fecal samples from howler monkeys (Alouatta palliata), spider monkeys (Ateles geoffroyi), tapirs (Tapirus bairdii) and felids (jaguars, Panthera onca; pumas, Puma concolor; jaguarundis, Puma yagouaroundi; and ocelots, Leopardus pardalis) living freely in two regions of the Mexican state of Veracruz under different degrees of human influence. Overall, our study shows that ATBR is commonplace in bacteria isolated from wildlife in southeast Mexico. Most of the resistances were towards old and naturally occurring antibiotics, but we also observed resistances of potential clinical significance. We found that proximity to humans positively affected the presence of ATBR and that ATBR was higher in terrestrial than arboreal species. We also found evidence suggesting different terrestrial and aerial routes for the transmission of ATBR between humans and wildlife. The prevalence and potential ATBR transfer mechanisms between humans and wildlife observed in this study highlight the need for further studies to identify the factors that might determine ATBR presence, abundance and distribution.

Leopardus

Wildlife disease

10.1371/journal.pone.0107719

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Citations (41)

ChemInform Abstract: Nucleophilic Reactions on 1‐Trifluoromethanesulfonylpyridinium Trifluoromethanesulfonate (Triflylpyridinium Triflate, TPT). Ring‐ Opening and “Unexpected” 1,4‐Dihydropyridine Reaction Products.

ChemInform (1997)

Rubén A. Toscano María Del Carmen Hernández-Galindo R. Rosas Olivia García‐Mellado Federico del Rı́o-Portilla

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Trifluoromethanesulfonate

10.1002/chin.199750146

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Citations (1)