[Objective] The study aimed to establish the high efficient tissue-culture regeneration system for the leaf of Chrysanthemum nankingense.[Method] With the leaves of C.nankingense as explants and MS medium as basic medium,10 kinds of media were designed by adding various hormone into MS medium so as to research the effect of various hormone combination and concentrations on induction rate of adventitious bud and rooting of Chrysanthemum nankingense leaves,and screen the optimal medium for callus induction,bud induction and rooting.[Result] The leaf explants of asepsis seedlings were cultured on the optimum medium of MS+2.0 mg/L 6-BA +0.2 mg/L NAA for inducing callus for 15 d,which got the induction of 98.0%,and then the callus with less explant were cultured on optimal medium of MS+1.0 mg/L 6-BA+0.5 mg/L NAA for bud induction,which got the budding rate of 94.0%,afterward the regenerated buds with length of over 1cm were cultured on optimal rooting medium of MS+IBA 0.05 mg/L,which got rooting rate of 100%,finally the seedlings that rooted for over 25 d had survival rate of over 95% after hardening and transplanting.[Conclusion] In the experiment,the tissue-culture regeneration technique procedure for C.nankingense leaf was established.
