Advanced nursing practice and education with a Master's degree as the necessary preparation, is viewed as a major strategy to cultivate senior nursing talents. Competencies are central to advanced nursing practice and education, but how can competencies be measured? Entrustable professional activities (EPAs) have been used widely in medicine as a practical approach for bridging the gaps between competency and clinical practice. Considering the paucity of research in EPAs for nursing graduates in China, it is needed to develop EPAs specifically for Chinese Master of Nursing Specialist (MNS) graduates to improve patient safety and quality patient care.
This study explored the relationship between rumination (deliberate or intrusive), emotional expressivity (positive or negative), and posttraumatic growth among patients within 1 month after an accidental injury. Using a cross-sectional design, 313 patients were investigated in two comprehensive hospitals. The results revealed that intrusive rumination had a significant positive effect on posttraumatic growth, partly through four different pathways: (i) the mediating role of deliberate rumination; (ii) the chain mediating role of deliberate rumination and negative emotional expressivity; (iii) the chain mediating role of negative emotional expressivity and positive emotional expressivity; and (iv) the chain mediating role of negative emotional expressivity, positive emotional expressivity, and deliberate rumination. Interventions to encourage emotional expressivity and facilitate the transition from intrusive rumination to deliberate rumination may promote posttraumatic growth soon after an accidental injury.
Pacritinib, an oral JAK kinase inhibitor, has recently been granted approval for the treatment of myelofibrosis and thrombocytopenia, making it a newly authorized drug within the pharmaceutical industry. In this study, a quantitative detection method for pacritinib in rat plasma was established by LC-MS/MS with Ibrutinib as an internal standard. Subsequently, this method was applied to pharmacokinetic and drug-drug interaction studies in rats. The established method showed good linearity in the range of 1-1500 ng/mL, with a lower limit of quantification (LLOQ) of 1 ng/mL. Furthermore, both intra-day and inter-day precision RSD% of Pacritinib were less than 10.69%, while the accuracy range was -2.31% to 2.08%. The recovery, matrix effect, and stability were consistent with the FDA guidelines. This method proved effective in quantitatively detecting pacritinib content in rat plasma. In the pharmacokinetic studies, we observed that isavuconazole significantly inhibited the metabolism of pacritinib compared to voriconazole. This led to a 2.5-fold increase in AUC(0-t), a 2.3-fold increase in AUC(0-∞), a 4.4-fold increase in CLz/F, and a 3.4-fold increase in Cmax, respectively. This study successfully established a reliable LC-MS/MS method for detecting pacritinib plasma drug concentration in rats. Pharmacokinetic studies showed that isavuconazole is more likely to increase the blood exposure of pacritinib than voriconazole.
There is growing evidence that the COVID-19 pandemic has had an impact on the mental health and professional identity of nursing students. This study aimed to explore the trajectories of psychological dis-tress and professional identity and examine their causal relationship among graduate nursing students. A prospective longitudinal study was conducted with 556 graduate nursing students in China who were asked to complete the Questionnaires for Emergent Events of Public Health and the Profes-sional Identity Questionnaire for Nursing Students at three timepoints dur-ing the pandemic (T1 = onset of the outbreak, T2 = state-wide isolation, T3 = school reopening). The results showed that psychological distress and psychological identity changed over time, with the lowest psychologi-cal distress and highest professional identity at T3. The cross-lagged analy-sis showed that professional identity at T1 and T2 negatively predicted psychological distress at T2 and T3. We concluded that nurse educators should develop programs and strategies to cultivate professional identity in graduate nursing education to improve mental health during public health crises. Cada vez hay más evidencia de que la reciente epidemia de que la COVID-19 puede afectar a la salud mental y la identidad profesional de los estudiantes de posgrado en enfermería. Es por ello que el propósito de este estudio fue explorar las trayectorias del estrés psicológico y la identidad ocupacional y examinar su relación causal de dichos estudiantes de posgra-do durante la nueva epidemia. Se llevó a cabo un estudio prospectivo longi-tudinal con 556 estudiantes de posgrado en enfermería en China, a los que se les pidió que completaran los cuestionarios de emergencia de salud pú-blica y el de identidad ocupacional para estudiantes de enfermería en tres etapas del brote (T1 = brote, T2 = aislamiento estatal, T3 = reapertura es-colar). Los resultados mostraron que estrés psicológico y la identidad psico-lógica de los estudiantes universitarios habían cambiado con el tiempo. En T3, la perturbación emocional fue la más baja y la identidad ocupacional la más alta. El análisis de retraso cruzado mostró que la identidad ocupacional de T1 y T2 tenía un efecto predictivo negativo sobre la perturbación psico-lógica de T2 y T3. Conclusión: los educadores de enfermería deberían for-mular programas y estrategias que cultiven la identidad profesional de sus estudiantes a fin de mejorar la salud mental en emergencias públicas.
In this paper, complexity measure based on Jensen-Shannon Divergence was used to compute complexity of the ECG signals, which include normal sinus rhythm, atrial premature contraction (APC) and sinus bradycardia (SBR) signals from the MIT-BIH standard database. The results show that three kinds of signals have different complexity measures. Normal sinus rhythm has the highest complexity, followed by sinus bradycardia (SBR) signals, and atrial premature contraction (APC) has the lowest complexity. It has good reference for clinical detecting and diagnosing with atrial premature contraction (APC) and sinus bradycardia (SBR) signals.
Objectives: To evaluate the level of parent-reported family resilience, parenting styles and psychosocial adjustment of children with chronic illness and to identify the relationships between family resilience, parenting styles and psychosocial adjustment in families with children with chronic illness. Methods: A cross-sectional study was conducted between June 2019 and August 2019. A total of 236 parents of children with chronic illness and 98 parents with healthy children were recruited from general hospitals by convenience sampling. A parent completed the Chinese Family Resilience Assessment Scale, the Parenting Rearing Patterns Questionnaire and the Strengths and Difficulties Questionnaire. Family resilience, parenting styles, and psychosocial adjustment of children with chronic illness were compared with those of healthy children. Structural Equation Modeling (SEM) was performed to explore the mediation effect of parenting styles between family resilience and psychosocial adjustment among children with chronic illness. Results: Parents of children with chronic illness reported lower level of family resilience and authoritative parenting, but more peer relationship problems compared to parents of healthy children. SEM showed that authoritative parenting fully mediated the relationship between family resilience and psychosocial adjustment of children with chronic illness. Conclusion: Childhood chronic illness reduces family resilience, authoritative parenting and children's psychosocial adjustment, but authoritative parenting mediated these effects, so authoritative parenting may be important for family resilience in families of children with chronic illness. Pediatric clinicians and nurses should provide family-centered interventions, as well as parenting training, to improve children's psychosocial outcomes.
This study aims to explore the prevalence of self-neglect and associated factors among older adults admitted to the hospital in the COVID-19 pandemic context. The cross-sectional study conducted at a Chinese comprehensive hospital between January and April 2021, 452 older adults were recruited to complete the Abrams Geriatric Self-Neglect Scale, Social Support Rate Scale, FRAIL scale, Barthel index, Patient Health Questionnaire-9, and 10-item Connor-Davidson Resilience Scale. Multivariate logistic regression was used to explore the factors associated with elder self-neglect. The results showed that the prevalence of self-neglect among our sample was 30.3%. Factors that were associated with the risk of elder self-neglect included male, having multiple children (≥4), receiving infrequent visits from children, frailty, and depression. There is a need to screen for self-neglect among older adults admitted to the hospital in the COVID-19 pandemic context. Tailored interventions are warranted to improve the quality of life of older adults.
Objective
To establish a homothermal and fast detecting method on pathogenic bacteria by combining recombinase-aid amplification (RAA) with molecular beacon.
Methods
The establishment of the methodology. Staphylococcus aureus specific primers were designed from the relative region of the staphylococcal protein A (SPA). Asymmetry amplification was optimized by adjusting the primer concentration ratios. The results of amplification and hybridization were visualized and analyzed by agarose gel electrophoresis and fluorescence detection. The sensitivity was identified by detecting dilute positive plasmids. And the specificity was determined using RAA method by detecting 72 pathogenic bacteria, including Staphylococcus aureus and other Staphylococcus spp. from the Department of Clinical Laboratory of Daping Hospital in December 2016. Besides, the Kappa analysis and the clinical diagnosis efficiency were investigated by analyzing 39 extra strains in the laboratory in December 2016.
Results
When the concentration ratio of restrictive and non-restrictive primer was 1∶20, the yield efficiency of single-stranded DNA (ssDNA) reached the peak. And as for the hybridization efficiency, the asymmetry amplification was higher than symmetry amplification. Twenty copies/μl was proposed as the limits of detection by testing dilute plasmids. And the RAA hybridization method could distinguish Staphylococcus aureus with other Staphylococcus spp.Comparing with traditional detection methods with a Kappa index of 0.860, this method shows a good consistency. By analyzing the 111 bacteria, the sensitivity of the method is 92.5% (37/40), the specificity is 97.2% (69/71), the positive predictive value is 94.9% (37/39), the negative predictive value is 95.8% (69/72), the positive likelihood radio is 33.04, the negative likelihood radio is 0.077, the Youden index is 0.897 and the Kappa index is 0.902.
Conclusion
Through the combination of asymmetry recombinase-aid amplification optimization and molecular beacon probe, a new method of detecting bacteria DNA with RAA hybridization technique is established, providing the foundation for its clinical application. (Chin J Lab Med, 2017, 40: 309-313)
Key words:
Staphylococcus aureus; Nucleic acid amplification techniques; Molecular probes
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)