Hirak Ranjan Dash

National Forensic Sciences University

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Surajit Das

National Institute of Technology Rourkela

Pankaj Shrivastava

Central Forensic Science Laboratory

Surajit Das

Midnapore Medical College and Hospital

Kamayani Vajpayee

Ahmedabad University

Noora Rashid Al-Snan

Kelly M. Elkins

Towson University

R. K. Kumawat

Central Forensic Science Laboratory

Neelam Mangwani

Heatherwood Hospital

Shivani Dixit

Central Forensic Science Laboratory

Kamlesh Kaitholia

Central Forensic Science Laboratory

Cooperative Institutions

National Institute of Technology Rourkela

Central Forensic Science Laboratory

Banaras Hindu University

Jadavpur University

Odisha University of Agriculture and Technology

KIIT University

Towson University

Life Sciences International Postgraduate Educational Center

Los Alamos National Laboratory

Indian Institute of Science Education and Research Kolkata

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Collection, Preservation, and Transportation of Biological Evidences

Springer eBooks (2021)

Hirak Ranjan Dash Kamayani Vajpayee

10.1007/978-981-15-9364-2_3-1

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Citations (2)

Isolation of DNA by Using Column-Based Extraction System

Springer protocols handbooks/Springer protocols (2020)

Hirak Ranjan Dash Pankaj Shrivastava Surajit Das

To isolate DNA from blood and forensic samples using column-based extraction system.

Isolation

10.1007/978-1-0716-0274-4_13

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Citations (2)

Guidelines for Collection and Preservation of Samples for Forensic DNA Testing

Springer eBooks (2020)

Pankaj Shrivastava Shivani Dixit Hirak Ranjan Dash

Dna testing

DNA profiling

10.1007/978-981-15-6655-4_29

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Citations (1)

Peopling of Rajasthan, India: Evaluating the gene flow from east and west

Gene Reports (2020)

R. K. Kumawat Pankaj Shrivastava Divya Shrivastava G.K. Mathur Hirak Ranjan Dash

10.1016/j.genrep.2020.100990

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Current Status and Advancements of Forensic DNA Analysis

Hirak Ranjan Dash Kelly M. Elkins Noora Rashid Al-Snan

DNA is widely used as a tool in criminal and civil legal cases. Forensic DNA typing and analysis has evolved and changed significantly in the past 5 years. The United States added additional STR loci in 2017, next generation sequencing (NGS) has been more widely applied for to forensic casework, and samples typed with NGS were approved for combined DNA index system (CODIS) upload in 2019. In this chapter, we describe the current status and advancements in forensic DNA analysis including applications for investigative genetic genealogy (IGG).

DNA profiling

Forensic Genetics

10.1007/978-981-99-6195-5_1

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Fast, High-Sensitive, and High-Resolution DNA Techniques

Hirak Ranjan Dash Kelly M. Elkins Noora Rashid Al-Snan

Upon the creation of the International Olympic Committee, the motto for the Olympic was proposed by Pierre de Coubertin. The adopted Olympic motto in the latinized version is "hendiatris Citius, Altius, Fortius" which can be translated as "Faster, Higher, Stronger." Criminal justice system expects an array of techniques with similar characteristics for the analysis of forensic exhibits to aid in the process of justice delivery. Most of the cases pertaining to the examination of biological samples are attributed to forensic DNA examination. The present-day DNA analysis techniques have seen much progress in terms of its reliability, robustness, sensitivity, and faster processing time. When the technique was invented in the year 1985 by Sir Alec Jeffreys, single or multilocus probes were in practice. Similarly, when the first STR kit was launched by Promega Corporation in 1994, only three STR loci were included in the multiplex system, i.e., CSF1PO, TPOX, and TH01 which was popularly called as CTT triplex. Gradually, the number of STR markers included in the multiplex systems increased and now-a-days, most of the STR multiplex kits have more than 20 STR markers. Inclusion of such a high number of STR markers provide high resolution to the technique by generating a high discrimination power, low matching probability, and many other useful forensic and paternity parameters.

Multiplex

STR multiplex system

Forensic Genetics

STR analysis

Robustness

10.1007/978-981-99-6195-5_5

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Role of Forensic DNA Databases in Criminal Identification

Hirak Ranjan Dash Kelly M. Elkins Noora Rashid Al-Snan

In this chapter, different national databases are discussed including the custody and the composition of the databases. The drawbacks of the databases are the negative effects on personal privacy and civil rights, and potential racial and medical discrimination. However, the role of forensic databases which can assist in resolving cold cases is an important contributor to preventing crime, increasing public safety, and promoting trust. Also, DNA mixtures with expanding STR loci can be beneficial in databases. Using partial matching, familial searching, and investigative genetic genealogy or forensic genetic genealogy can highly assist in identifying suspects from blood-related individuals for whom DNA reference or comparison samples are stored in the database. Databases are crucial in reopening cold cases and offering rightful convictions to people whom were wrongfully arrested for many years and putting the real suspects behind bars.

Identification

Forensic Genetics

Crime scene

DNA profiling

10.1007/978-981-99-6195-5_9

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Quantification of DNA by Using qRT-PCR

Springer protocols handbooks/Springer protocols (2020)

Hirak Ranjan Dash Pankaj Shrivastava Surajit Das

To assess the quality and quantity of DNA isolated from blood and forensic samples using qRT-PCR technique.

10.1007/978-1-0716-0274-4_17