Frank Soldner

Research Network (United States)

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Dirk Hockemeyer

University of California, Berkeley

Rudolf Jaenisch

Whitehead Institute for Biomedical Research

Hanqin Li

University of California, Berkeley

Oriol Busquets

Universidad Rovira i Virgili

Yogendra Verma

King George's Medical University

Steven Poser

Albert Einstein College of Medicine

Khaja Syed

Rajiv Gandhi Centre for Biotechnology

Donald C. Rio

Research Network (United States)

Helen S. Bateup

University of California, Berkeley

Gabriella Pangilinan

University of California, Berkeley

Cooperative Institutions

Massachusetts Institute of Technology

Harvard University

Whitehead Institute for Biomedical Research

University of California, Berkeley

Howard Hughes Medical Institute

Brigham and Women's Hospital

Research Network (United States)

Scripps Research Institute

Sanford Burnham Prebys Medical Discovery Institute

Discovery Institute

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Creating Patient-Specific Neural Cells for the In Vitro Study of Brain Disorders

Stem Cell Reports (2015)

Kristen Brennand M. Carol Marchetto Nissim Benvenisty Oliver Brüstle Allison D. Ebert

Brain disease

10.1016/j.stemcr.2015.10.011

Cite

Citations (76)

Human embryonic stem cells with biological and epigenetic to those of mouse ESCs

Proceedings of the National Academy of Sciences (2010)

Jacob H. Hanna Aixin Cheng Krishanu Saha Ji Hyeon Kim Christopher J. Lengner

Embryoid body

Source

Cite

Citations (0)

Preparation of mouse embryonic fibroblast (MEF) feeder plates for hPSC cultures v1

Hanqin Li Oriol Busquets Steven Poser Dirk Hockemeyer Frank Soldner

This protocol describes the preparation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture. Protocol overview A. Starting with frozen irradiated or Mitomycin C inactivated MEFs (optional) B. Starting with fresh irradiated or Mitomycin C inactivated MEFs General notes 1. Throughout this protocol, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs. 2. Either fresh (start at step 4) or frozen stocks of irradiated or Mitomycin C inactivated MEFs can be used to prepare hPSC feeder cells. 3. The indicated MEF density are recommended starting densities and might have to be adjusted for each hPSC line and hPSC medium formulation (KSR, serum-free versus serum-containing medium). 4. MEFs were obtained as described in Manipulating the Mouse Embryo: A Laboratory Manual, Third Edition (ISBN: 0879695919)

Mitomycin C

10.17504/protocols.io.b4n9qvh6

Cite

Citations (0)

Differential effects of l-buthionine sulfoximine and ethacrynic acid on glutathione levels and mitochondrial function in PC12 cells

Neuroscience Letters (1999)

Jan Seyfried Frank Soldner Jörg B. Schulz Thomas Klockgether K.‐A. Kovar

Buthionine sulfoximine

10.1016/s0304-3940(99)00107-x

Cite

Citations (75)

Mitomycin C inactivation of mouse embryonic fibroblasts (MEFs) for hPSC cultures v1

Hanqin Li Oriol Busquets Steven Poser Dirk Hockemeyer Frank Soldner

This protocol describes the process of using Mitomycin C to inactivate mouse embryonic fibroblasts (MEFs), which can then be used as feeder cells for human pluripotent stem cell (hPSC) culture. General notes 1. Throughout this protocol, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs. 2. MEFs were obtained as described in Manipulating the Mouse Embryo: A Laboratory Manual, Third Edition (ISBN: 0879695919)

Mitomycin C

10.17504/protocols.io.b4n8qvhw

Cite

Citations (0)

Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease

Proceedings of the National Academy of Sciences (2008)

Marius Wernig Jian-Ping Zhao Jan Pruszak Eva Hedlund Dongdong Fu

The long-term goal of nuclear transfer or alternative reprogramming approaches is to create patient-specific donor cells for transplantation therapy, avoiding immunorejection, a major complication in current transplantation medicine. It was recently shown that the four transcription factors Oct4, Sox2, Klf4, and c-Myc induce pluripotency in mouse fibroblasts. However, the therapeutic potential of induced pluripotent stem (iPS) cells for neural cell replacement strategies remained unexplored. Here, we show that iPS cells can be efficiently differentiated into neural precursor cells, giving rise to neuronal and glial cell types in culture. Upon transplantation into the fetal mouse brain, the cells migrate into various brain regions and differentiate into glia and neurons, including glutamatergic, GABAergic, and catecholaminergic subtypes. Electrophysiological recordings and morphological analysis demonstrated that the grafted neurons had mature neuronal activity and were functionally integrated in the host brain. Furthermore, iPS cells were induced to differentiate into dopamine neurons of midbrain character and were able to improve behavior in a rat model of Parkinson's disease upon transplantation into the adult brain. We minimized the risk of tumor formation from the grafted cells by separating contaminating pluripotent cells and committed neural cells using fluorescence-activated cell sorting. Our results demonstrate the therapeutic potential of directly reprogrammed fibroblasts for neuronal cell replacement in the animal model.

Reprogramming

Nestin

KLF4

10.1073/pnas.0801677105

Cite

Citations (1,198)

Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment

PMC (2018)

Saranna Fanning Aftabul Haque Thibaut Imberdis Valeriya Baru M. Inmaculada Barrasa

Neurotoxicity

Homeostasis

Fatty Acid Metabolism

Source

Cite

Citations (0)

Generation of Isogenic Pluripotent Stem Cells Differing Exclusively at Two Early Onset Parkinson Point Mutations

Cell (2011)

Frank Soldner Josée Laganière Albert Cheng Dirk Hockemeyer Qing Gao

10.1016/j.cell.2011.06.019

Cite

Citations (703)

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

Nature Biotechnology (2009)

Dirk Hockemeyer Frank Soldner Caroline Beard Qing Gao Maisam Mitalipova

Zinc finger nuclease

Gene targeting

10.1038/nbt.1562

Cite

Citations (1,043)

Transgene Excision Has No Impact on In Vivo Integration of Human iPS Derived Neural Precursors

PLoS ONE (2011)

Tamara Major Jayanthi Menon Gordon Auyeung Frank Soldner Dirk Hockemeyer

The derivation of induced human pluripotent stem cells (hiPS) has generated significant enthusiasm particularly for the prospects of cell-based therapy. But there are concerns about the suitability of iPS cells for in vivo applications due in part to the introduction of potentially oncogenic transcription factors via viral vectors. Recently developed lentiviral vectors allow the excision of viral reprogramming factors and the development of transgene-free iPS lines. However it is unclear if reprogramming strategy has an impact on the differentiation potential and the in vivo behavior of hiPS progeny. Here we subject viral factor-free, c-myc-free and conventionally reprogrammed four-factor human iPS lines to a further challenge, by analyzing their differentiation potential along the 3 neural lineages and over extended periods of time in vitro, as well as by interrogating their ability to respond to local environmental cues by grafting into the striatum. We demonstrate similar and efficient differentiation into neurons, astrocytes and oligodendrocytes among all hiPS and human ES line controls. Upon intracranial grafting in the normal rat (Sprague Dawley), precursors derived from all hiPS lines exhibited good survival and response to environmental cues by integrating into the subventricular zone, acquiring phenotypes typical of type A, B or C cells and migrating along the rostral migratory stream into the olfactory bulb. There was no teratoma or other tumor formation 12 weeks after grafting in any of the 26 animals used in the study. Thus neither factor excision nor persistence of c-myc impact the behavior of hiPS lines in vivo.

Reprogramming

10.1371/journal.pone.0024687

Cite

Citations (18)