Gabriela Chaufan

Fundación Ciencias Exactas y Naturales

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María del Carmen Ríos de Molina

University of Buenos Aires

Isis Coalova

University of Buenos Aires

Nancy Beatriz Andrioli

Fundación Ciencias Exactas y Naturales

Martín Ricardo Ventureira

Experimental Medicine and Biology Institute

Sebastián E. Sabatini

University of Buenos Aires

Elisa Cebral

Experimental Medicine and Biology Institute

Tamara Anahí Coll

National University of General San Martín

Leticia Pérez‐Tito

Fundación Ciencias Exactas y Naturales

ÁB

Ángela Beatriz Juárez

Instituto de Biodiversidad y Biología Experimental y Aplicada

Leonor C. San Martín de Viale

Fundación Ciencias Exactas y Naturales

Cooperative Institutions

University of Buenos Aires

Fundación Ciencias Exactas y Naturales

Consejo Nacional de Investigaciones Científicas y Técnicas

University of California System

Institute of Astronomy and Space Physics

Experimental Medicine and Biology Institute

Centro Científico Tecnológico - Tucumán

Centro Científico Tecnológico - San Juan

Instituto de Química y Fisicoquímica Biológicas

Instituto de Biodiversidad y Biología Experimental y Aplicada

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Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis

Molecular and Cellular Biochemistry (2017)

Tamara Anahí Coll Gabriela Chaufan Leticia Pérez‐Tito Martín Ricardo Ventureira María del Carmen Ríos de Molina

Trophoblast

Myometrium

Nitrotyrosine

Decidual cells

Decidua

10.1007/s11010-017-3158-y

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Citations (19)

Abnormal kinetic behavior of uroporphyrinogen decarboxylase obtained from rats with hexachlorobenzene‐induced porphyria

Journal of Biochemical and Molecular Toxicology (2005)

Gabriela Chaufan María M. Corvi Leonor C. San Martín de Viale Marı́a Luz Cárdenas María del Carmen Ríos de Molina

Abstract Uroporphyrinogen decarboxylase is an essential enzyme in all organisms and functions in the heme biosynthetic pathway, catalyzing the decarboxylation of the four acetate groups of uroporphyrinogen to form coproporphyrinogen. This work examines whether the four sequential decarboxylations occur at the same active site, and explores whether hexachlorobenzene‐induced porphyria affects the behavior of the enzyme. For this purpose, kinetic competition studies were done with mixtures of uroporphyrinogen III and pentacarboxyporphyrinogen III. With the enzyme from normal rats, a constant velocity was obtained with all the mixtures, indicating that uroporphyrinogen and pentacarboxy‐porphyrinogen react at the same active site, i.e. the first and fourth decarboxylations occur at the same site. In contrast, in experiments with enzyme from rats with hexachlorobenzene‐induced porphyria, the total rate for mixtures was always lower than the reference rate; and a curve with a deep minimum was obtained, indicating that the two reactions occur at functionally different sites, but with cross‐inhibition. This suggests that the modifications induced in the enzyme by hexachlorobenzene cause the two active sites to become nonequivalent and functionally different. The question is discussed how the hexachlorobenzene treatment may produce this abnormal kinetic behavior, and alternative hypotheses are considered. © 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:19–24, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20055

Hexachlorobenzene

Uroporphyrinogen III decarboxylase

Decarboxylation

10.1002/jbt.20055

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Citations (4)

HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic

Journal of Basic and Applied Genetics (2017)

Nancy Beatriz Andrioli Gabriela Chaufan Isis Coalova M. Carmen Molina Marta Dolores Mudry

Fil: Andrioli, Nancy Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Ecologia, Genetica y Evolucion de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecologia, Genetica y Evolucion de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Grupo de Investigacion de Biologia Evolutiva; Argentina

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Dietary copper effects in the estuarine crab, Neohelice (Chasmagnathus) granulata, maintained at two different salinities

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology (2009)

Sebastián E. Sabatini Gabriela Chaufan Ángela Beatriz Juárez Isis Coalova Laura Bianchi

Copper toxicity

10.1016/j.cbpc.2009.07.006

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Citations (36)

Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation

Toxicology in Vitro (2014)

Isis Coalova María del Carmen Ríos de Molina Gabriela Chaufan

10.1016/j.tiv.2014.06.014

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Citations (46)

Oxidative stress and cellular and tissue damage in organogenic outbred mouse embryos after moderate perigestational alcohol intake

Molecular Reproduction and Development (2017)

Tamara Anahí Coll Gabriela Chaufan Leticia Pérez‐Tito Martín Ricardo Ventureira Cristián Sobarzo

Perigestational alcohol consumption by CF‐1 mouse, from before mating up to the period of embryo organogenesis, leads to retarded early embryo development and neural tube defects. Here, we addressed if perigestational alcohol ingestion up to Day 10 of pregnancy induces oxidative stress and changes in macromolecules and organ tissues of early organogenic embryos. Adult CF‐1 female mice were administered 10% ethanol in their drinking water for 17 days prior to mating and until Day 10 of gestation, whereas control females were administered ethanol‐free water. Our results demonstrated significantly reduced Catalase abundance and activity and increased glutathione content in the embryos of ethanol‐treated females. The nitrite level was significantly reduced, but TBARS (thiobarbituric acid reactive substances) content, an index of lipid peroxidation, did not change. Embryos derived from ethanol‐treated females also showed higher abundance of 3‐nitrotyrosine (3‐NT)‐containing proteins in all tissues, compared to the control group. Apoptosis was significantly increased in the ectoderm and mesoderm, but not in the heart—although this organ did contain more cleaved Caspase‐3‐positive cardiomyocytes per area of ventricular myocardium than controls. In sum, moderate perigestational alcohol ingestion up to Day 10 of gestation in mice induces oxidative stress by altering radical nitrogen species and antioxidant enzymatic and non‐enzymatic mechanisms in embryos. Further, generalized protein nitration, due to unbalanced nitric oxide levels associated with tissue‐specific apoptosis, was detected in embryos, suggesting that oxidative mechanisms may play an important role in the perigestational alcohol‐induced malformation of organogenic embryos exposed to ethanol.

TBARS

Nitrotyrosine

10.1002/mrd.22865

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Citations (12)

Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.

PubMed (1996)

M C Ríos de Molina Gabriela Chaufan Sara Iglesias S Billi de Catabbi Leonor C. San Martín de Viale

Porphyrinogen carboxylyase from normal rat liver was subjected to purification methods. Two different purification protocols were used. In both cases, the initial steps consisted in obtaining a liver homogenate, followed by centrifugation, salt precipitation and phosphate gel absorption. Scheme I consisted in then submitted the preparation to DEAE-cellulose, followed by Sephacryl S-200 and Phenyl-sepharose sequential column chromatographies. Scheme II involved an affinity column followed by a Sephadex G-75 gel filtration column. In both cases, the enzyme was stored at -20 degrees C until its assay. The addition of 2mM dithiotreytol to the incubation media or to the enzyme extract before storage, did not help improve the activity nor the stability of the enzyme. Those fractions containing the maximal enzyme activity, detected using Uroporphyrinogen III or Pentacarboxy-porphyrinogen III as substrate, were not always present in the same tubes for the different columns employed. In addition, the degree of purification obtained in some steps was different according to the substrate employed. The results suggest the existence of at least two isoenzymes for rat liver porphyrinogen carboxy-lyase.

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Specific activity

Sepharose

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Citations (3)

Individual and joint effects of glyphosate and cypermethrin formulations on two human cell lines

Toxicology and Applied Pharmacology (2023)

Isis Coalova Hugo March María del Carmen Ríos de Molina Gabriela Chaufan

10.1016/j.taap.2023.116398

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Citations (2)

Genotoxic effects induced for sub-cytotoxic concentrations of tebuconazole fungicide in HEp-2 cell line

Chemico-Biological Interactions (2023)

Nancy Beatriz Andrioli Mariela Nieves Mariel Poltronieri Chiara Bonzon Gabriela Chaufan

Comet Assay

Tebuconazole

Hep G2

10.1016/j.cbi.2023.110385

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Citations (6)

In vitro effects of endosulfan-based insecticides on mammalian sperm

Toxicology Research (2017)

Melisa Sanchez C. Alvarez Sedó Gabriela Chaufan Marina Romanato Rodrigo Da Cuña

Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensation could be altered by endosulfan in mice sperm. Spermatozoa from cauda epididymis were obtained from mature male mice and incubated in the presence of two commercial formulations (CFs) of endosulfan (Master® and Zebra Ciagro®) or the active ingredient (AI) alone. A significant decrease in the percentage motility and viability of spermatozoa with respect to controls was found. In vitro decondensation was performed in the presence of glutathione and heparin. Spermatozoa incubated with the AI, endosulfan Master® and endosulfan Zebra Ciagro® showed an increase in chromatin decondensation. In addition, the TUNEL assay showed that DNA fragmentation was significantly higher when sperm were incubated with either one of the CFs when compared to the AI or controls. The ultrastructure analysis of sperm cells showed evident changes in the structure of the plasma and acrosome membranes of sperm incubated with endosulfan AI or the CFs. These results suggest that endosulfan can affect sperm integrity and in vitro chromatin decondensation as well as DNA fragmentation.

Endosulfan

Fragmentation

10.1039/c7tx00251c

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Citations (8)