The purpose of this study was to demonstrate the ability of computed microtomography based on monochromatic synchrotron radiation (SRmuCT) in microstructural analysis of cortical bone. Tibial diaphyses of growing rats (14 wk, n = 8) undergoing unilateral sciatic neurectomy 8 wk before study were imaged with spatial volume resolution of 5.83 x 5.83 x 5.83 microm3 by SRmuCT (20 keV) at the synchrotron radiation facility (SPring-8). Reconstructed image data were translated into local mineral densities by using a calibrated linear relationship between linear absorption coefficients and concentrations of homogeneous K2HPO4 solution. Pure bone three-dimensional images, produced by simple thresholding at a bone mineral density of 0.82 g/cm3, were analyzed for macro- and microscopic structural properties. In neurectomized hindlimbs, cortical canal network rarefaction as well as bone atrophy were found. The former was characterized by 30% smaller porosity, 11% smaller canal density in transverse section, and 38% smaller canal connectivity density than those in contralateral bone. On the other hand, no difference was found in bone mineral density between neurectomized and intact hindlimbs (1.37 vs. 1.36 g/cm3). In conclusion, SRmuCT is a promising method for the three-dimensional analysis of cortical microstructure and the degree of mineralization in small animals.
Regional myocardial flow distributions in Langendorff rat hearts under Tyrode and blood perfusion were assessed by tracer digital radiography (100-microm resolution). Flow distributions during baseline and maximal hyperemia following a 60-s flow cessation were evaluated by the coefficient of variation of regional flows (CV; related to global flow heterogeneity) and the correlation between adjacent regional flows (CA; inversely related to local flow randomness). These values were obtained for the original images (64(2) pixels) and for coarse-grained images (32(2), 16(2), and 8(2) blocks of nearby pixels). At a given point in time during baseline, both CV and CA were higher in blood (n = 7) than in Tyrode perfusion (n = 7) over all pixel aggregates (P < 0.05, two-way ANOVA). During the maximal hyperemia, CV and CA were still significantly higher in blood (n = 7) than in Tyrode perfusion (n = 7); however, these values decreased substantially in blood perfusion and the CV and CA differences became smaller than those at baseline accordingly. During basal blood perfusion, the 60-s average flow distribution (n = 7) showed a smaller CV and CA than those at a given point in time (P < 0.05, two-way ANOVA). Coronary flow reserve was significantly higher in blood than in Tyrode perfusion. In conclusion, the flow heterogeneity and the local flow similarity are both higher in blood than in Tyrode perfusion, probably due to the different degree of coronary tone preservation and the presence or absence of blood corpuscles. Under blood perfusion, temporal flow fluctuations over 60-s order are largely involved in shaping microregional flow distributions.
The purpose of this study was to test the hypothesis that exchange transfusion with liposomal hemoglobin (LH) reduces the microheterogeneity of regional myocardial flows while sustaining cardiac function. Neo Red Cell mixed with albumin was used as the LH solution, in which the LH volume fraction was 17∼18% and hemoglobin density was nearly two-thirds smaller than in rat blood. Regional myocardial flows in left ventricular free walls were measured by tracer digitalradiography (100-μm resolution) in anesthetized rats with or without 50% blood-LH exchange transfusion. Within-layer flow distributions showed lower heterogeneity with ( n = 8) than without ( n = 8) LH transfusion. No extravasation of hemoglobin was confirmed by 3,3-diaminobenzidin staining ( n = 2). Carotid flow increased by 68% due to LH transfusion, whereas arterial pressure and heart rate remained unchanged. On the other hand, cross-circulated rat hearts ( n = 7) were used to evaluate the effects of 50% blood-LH exchange on coronary flow and tone preservation under 300-beats/min pacing and 100-mmHg perfusion pressure. Blood-LH exchange caused a 71% increase of coronary flow and 10% decrease of percent flow increase during hyperemia after 30-s flow interruption. Myocardial O 2 supply and consumption increased by 9% and 10%, respectively, whereas myocardial O 2 extraction remained unchanged. The large increases of in vivo carotid flow and coronary flow in cross-circulated hearts due to LH coperfusion could be explained by the reduction of apparent flow viscosity. These results suggest that under LH coperfusion, the microheterogeneity of myocardial flows decreases with increased coronary flow while fairly preserving coronary tone and cardiac function.
