Shu‐Lin Liu

Nankai University

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Zhi‐Gang Wang

Nankai University

Dai‐Wen Pang

Nankai University

Zhiling Zhang

Sun Yat-sen University Cancer Center

Haoyang Liu

Fuzhou University

Yusi Hu

Nankai University

Hua‐Jie Chen

China University of Geosciences

Enze Sun

University of Miami

Zhi‐Quan Tian

Tibet University

Xue‐Hui Shi

Nankai University

Hua‐Lin Wu

National Cheng Kung University

Cooperative Institutions

Chinese Academy of Sciences

104

Wuhan University

Nankai University

State Key Laboratory of Medicinal Chemical Biology

University of Chinese Academy of Sciences

Peking University

Central South University

Harbin Medical University

National Cheng Kung University

Chinese Academy of Medical Sciences & Peking Union Medical College

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Ratiometric Fluorescence Probes for In Situ Imaging of Membrane Tension in Live Cells

Bioconjugate Chemistry (2024)

Haiyan Wen Yusi Hu You‐Yang Duo Liang Zhao Zhi‐Gang Wang

Membrane tension is an important physical parameter of describing cellular homeostasis, and it is widely used in the study of cellular processes involving membrane deformation and reorganization, such as cell migration, cell spreading, and cell division. Despite the importance of membrane tension, direct measurement remains difficult. In this work, we developed a ratiometric fluorescent probe sensitive to membrane tension by adjusting the carbon chain structure based on polarity-sensitive fluorophores. The probe is sensitive to changes in membrane tension after cells were subjected to physical or chemical stimuli, such as osmotic shock, lipid peroxidation, and mechanical stress. When the polarity of the plasma membrane increases (the green/red ratio decreases) and the membrane tension increases, the relative magnitude of the membrane tension can be quantitatively calculated by fluorescence ratio imaging. Thus, the probe proved to be an efficient and sensitive membrane tension probe.

10.1021/acs.bioconjchem.4c00101

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Comparative analysis of SARS-CoV-2 and its receptor ACE2 with evolutionarily related coronaviruses

Aging (2020)

Fei-Feng Li Qiong Zhang Gui-Yu Wang Shu‐Lin Liu

To the Editor,The current pandemic COVID-19 is rapidly spreading worldwide.This disease is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; previously called 2019-nCoV), seriously threatening the human health [1].Since december 12, 2019, when the first patient was confirmed [2], more than 20 million cases have been confirmed, with over 740,000 deaths globally.Due to the rapidly increasing numbers of confirmed cases and deaths of COVID-19, the WHO has raised the risk of spread and impact of this disease to a very high level [1, 2].Coronaviruses, first described from the common cold patients in 1966, are enveloped positive single-stranded RNA nuclear viruses, which can infect a large variety of host species including humans [3,4].SARS-CoV-2 is a www.aging-us.com

Pandemic

Coronavirus

2019-20 coronavirus outbreak

Betacoronavirus

Common cold

10.18632/aging.104024

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Citations (3)

The Impact of TiO <sub>2</sub> Nanoparticle Exposure on Transmembrane Cholesterol Transport and Enhanced Bacterial Infectivity

SSRN Electronic Journal (2021)

Fan Yang Shu‐Lin Liu Yan Xu Stephen G. Walker Wonhwa Cho

We have previously shown that exposure to TiO2 nanoparticles (NPs) reduces the resistance of HeLa cells to bacterial infection. Here we demonstrate that the increased infectivity is associated with enhanced asymmetry in the cholesterol distribution. We applied a new live cell imaging method which uses tunable orthogonal cholesterol sensors to visualize and quantify in-situ cholesterol distribution between the two leaflets of the plasma membrane (PM). In the control culture, we found marked transbilayer asymmetry of cholesterol, with the concentration in the outer plasma membrane (OPM) being 12.5(2.2)-fold higher than that in the inner plasma membrane (IPM). Exposure of the culture to 0.1 mg/mL of rutile TiO2 NPs increased the asymmetry such that the concentration in the OPM was 50.8(9.5) times higher, while the total cholesterol content increased only 20.5(2.4)%. This change in cholesterol gradient may explain the increase in bacterial infectivity in HeLa cells exposed to TiO2 NPs since many pathogens, including Staphylococcus aureus used in the present study, require cholesterol for proper membrane attachment and virulence. RT-PCR indicated that exposure to TiO2 was responsible for upregulation of the ABCA1 and ABCG1 mRNAs, which are responsible for the production of the cholesterol transporter proteins that facilitate cholesterol transport across cellular membranes. This was confirmed by the observation of an overall decrease in bacterial infection in ABCA1 knockout or methyl-Î²-cyclodextrin-treated HeLa cells, as regardless of TiO2 NP exposure. Hence rather than preventing bacterial infection, TiO2 nanoparticles upregulate genes associated with membrane cholesterol production and distribution, hence increasing infectivity.

Infectivity

10.2139/ssrn.3813471

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Myeloid Cell 5-Lipoxygenase Activating Protein Modulates the Response to Vascular Injury

Circulation Research (2012)

Yu Zhou Emanuela Ricciotti Takashi Miwa Shu‐Lin Liu Kaori Ihida‐Stansbury

Human genetics have implicated the 5-lipoxygenase enzyme in the pathogenesis of cardiovascular disease, and an inhibitor of the 5-lipoxygenase activating protein (FLAP) is in clinical development for asthma.

Knockout mouse

Neointima

10.1161/circresaha.112.300755

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Citations (10)

NIR-II Fluorophores: From Synthesis to Biological Applications

Journal of Analysis and Testing (2023)

You‐Yang Duo Liang Zhao Zhi‐Gang Wang Shu‐Lin Liu

Autofluorescence

Biological Imaging

Fluorescence-lifetime imaging microscopy

10.1007/s41664-023-00254-2

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Citations (15)

Hydrogen-rich PBS protects cultured human cells from ionizing radiation-induced cellular damage

Nuclear Technology and Radiation Protection (2010)

Liren Qian Bailong Li Fei Cao Yuecheng Huang Shu‐Lin Liu

Hydroxyl radicals play an important role in ionizing radiation-induced cellular damage, while hydrogen can selectively reduce hydroxyl radicals in vitro. This study was designed to test the hypothesis that hydrogen-rich PBS may be an effective radioprotective agent in vitro. Compared to cells pretreated without hydrogen, we demonstrated that treating cells with hydrogen-rich PBS before irradiation could significantly inhibit IR-induced apoptosis, increase viability of human intestinal crypt cells, significantly increase endogenous antioxidant, and decrease malondialdehyde and 8-hydroxydeoxyguanosine concentrations of human lymphocyte AHH-1 cells. It is concluded that hydrogen has a potential as an effective and safe radioprotective agent.

Malondialdehyde

Hydroxyl radical

10.2298/ntrp1001023q

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Citations (19)

Perfluorocarbon-facilitated CNS oxygen toxicity in rats: Reversal by edaravone

Brain Research (2012)

Shu‐Lin Liu Runping Li Xiaoxiao Ni Zhiyu Cai Rongjia Zhang

Edaravone

Malondialdehyde

10.1016/j.brainres.2012.06.051

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Citations (12)

Near‐Infrared II Hemicyanine Dye with Large Stokes Shift Designed by TICT Regulation for Boosting Imaging‐Guided Photothermal Therapy

Advanced Healthcare Materials (2023)

Liang Zhao Han Zhu You‐Yang Duo Dai‐Wen Pang Zhi‐Gang Wang

The serious threat that cancer poses to human health highlights the significance of early detection and effective treatment. The integration of fluorescence diagnosis and photothermal therapy in NIR-II has gained attention due to its high sensitivity, fast response, and noninvasiveness. Fluorescence, produced by the radiative relaxation process of electrons in a molecule, and photothermal, generated by the nonradiative relaxation process of electrons in a molecule, are competing photophysical processes. Hence, it is a challenge for the molecule to balance between the properties of fluorescence and photothermal. In this study, a NIR-II hemicyanine with TICT character is designed to obtain molecules with both better fluorescence and photothermal properties, utilizing positively charged pyridine salt and triphenylamine as electron acceptor and donor, respectively, and oxole as the conjugated π-bridge. HCY-995, one of the synthesized compounds, has a quantum yield of 0.09%, photothermal conversion efficiency of 54.90%, and a significant Stoke shift of 232 nm, which makes it appropriate for the integration of photothermal therapy and high-resolution imaging. This study provides new insights into the development of NIR-II molecules with fluorescent and photothermal integrated properties.

Triphenylamine

Photothermal effect

Quantum yield

10.1002/adhm.202301584

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Citations (5)

Proton transfer induced persistent triplet charge transfer phosphorescence in molecule-doped polymer systems

Chemical Engineering Journal (2024)

Shu‐Lin Liu Chensheng Li Liyun Liao Yanping Huo Zhong‐Min Su

10.1016/j.cej.2024.154819

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Citations (1)

N-Cadherin Induction by ECM Stiffness and FAK Overrides the Spreading Requirement for Proliferation of Vascular Smooth Muscle Cells

Cell Reports (2015)

Keeley L. Mui Yongho Bae Lin Gao Shu‐Lin Liu Tina Xu

In contrast to the accepted pro-proliferative effect of cell-matrix adhesion, the proliferative effect of cadherin-mediated cell-cell adhesion remains unresolved. Here, we studied the effect of N-cadherin on cell proliferation in the vasculature. We show that N-cadherin is induced in smooth muscle cells (SMCs) in response to vascular injury, an in vivo model of tissue stiffening and proliferation. Complementary experiments performed with deformable substrata demonstrated that stiffness-mediated activation of a focal adhesion kinase (FAK)-p130Cas-Rac signaling pathway induces N-cadherin. Additionally, by culturing paired and unpaired SMCs on microfabricated adhesive islands of different areas, we found that N-cadherin relaxes the spreading requirement for SMC proliferation. In vivo SMC deletion of N-cadherin strongly reduced injury-induced cycling. Finally, SMC-specific deletion of FAK inhibited proliferation after vascular injury, and this was accompanied by reduced induction of N-cadherin. Thus, a stiffness- and FAK-dependent induction of N-cadherin connects cell-matrix to cell-cell adhesion and regulates the degree of cell spreading needed for cycling.

VE-cadherin

10.1016/j.celrep.2015.02.023

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Citations (72)