S Hatsushika

Hiroshima University

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Nucleation Time and Fatty Acid Composition of Lecithin in Human Gallbladder Bile

Scandinavian Journal of Gastroenterology (1993)

S Hatsushika Susumu Tazuma Goro Kajiyama

AbstractWe investigated the relationship between bile nucleation time and the fatty acid composition of biliary lecithin in human gallbladder bile. Bile samples from patients with cholesterol gallstones nucleated more rapidly than those from patients with noncholesterol gallstones or no stones. The biliary cholesterol concentration was highest in the cholesterol gallstone group and was correlated with the molar percentage of linoleic acid and arachidonic acid, with these percentages also being higher in bile from the cholesterol gallstone patients than in bile from the other two groups. In addition, the mucous glycoprotein concentration in bile was also significantly higher in the cholesterol gallstone group. Thirty-three patients in the no-stone group showed bile nucleation times of less than 21 days. Higher concentrations of cholesterol and mucous glycoprotein and higher molar percentages of arachidonic and linoleic acid were noted in these patients. These findings suggest that in humans, hepatic cholesterol hypersecretion is associated with the increased unsaturated fatty acid proportion in biliary phospholipids and gallbladder mucin hypersecretion, thereby causing rapid cholesterol crystal nucleation.Key Words: Cholesterol gallstonesfatty acid composition of biliary lecithingallbladder mucin hypersecretionnucleation time

10.3109/00365529309096059

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Citations (22)

[A study on the mechanisms whereby apolipoprotein A-1 (apo A-1) inhibits cholesterol crystal nucleation in human gallbladder bile].

PubMed (1991)

Susumu Tazuma Shigeki Mizuno Hiroshi Sasaki Hiroaki Sagawa S Hatsushika

The aim of the present study was to determine the mechanisms whereby apo A-1 retards cholesterol crystal nucleation in human gallbladder bile. Ten human gallbladder bile samples were sterilely collected from patients with gallstones at surgery, subsequently applied on gel permeation chromatography to separate various lipid particles. A non-micellar fraction coeluted with apo A-1 was ultrastructurally characterized by transmission electron microscopy (TEM). As results, that fraction containing very little or no apo A-1, 400-600 A in diameter, showed rapid transformation, i.e. rouleau formation, multilamellar formation and consequently microcrystal formation, whereas those rich in apo A-1, 200-400 A in diameter, showed very little transformation for 7 days. Those data indicated that apo A-1 stabilizes non micellar fraction by forming an apo A-1 and lipid complexed particle, resulting in prolonged cholesterol crystal nucleation.

Gel permeation chromatography

Crystal (programming language)

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Citations (3)

Effects of long-term treatment with low-dose pravastatin on biliary lipid and bile acid composition in patients with nonfamilial hyperlipoproteinemia

Metabolism (1995)

Susumu Tazuma Itsuo Takizawa Tetsuro Kunita Toshiyuki Mizuno T. Watanabe

10.1016/0026-0495(95)90138-8

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Citations (14)

[Effects of a competitive inhibitor of hydroxymethylglutaryl coenzyme A reductase on cholesterol gallstone formation in prairie dogs].

PubMed (1991)

Susumu Tazuma S Hatsushika Naoki Aihara Y Gunji H Sasaki

Coenzyme A

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Citations (1)

Simultaneous microanalysis of biliary cholesterol, bile acids and fatty acids in lecithin using capillary column gas chromatography: an advantage to assess bile lithogenecity

Journal of Chromatography B Biomedical Sciences and Applications (1994)

Susumu Tazuma S Hatsushika Gunji Yamashita Naoki Aihara Masatoshi Sasaki

Degree of unsaturation

Glycerophospholipids

10.1016/0378-4347(93)e0422-m

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Citations (27)

Factors affecting bile lecithin hydrophobicity: Implication of bile cholesterol metastability during organic anion-induced cholestasis

Gastroenterology (1995)

Susumu Tazuma Haruki Ochi Hiroyuki Miura K Horikawa Hiroaki Miyake

Metastability

10.1016/0016-5085(95)29038-9

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Citations (0)

Inhibitory Effects of Pravastatin, a Competitive Inhibitor of Hydroxymethylglutaryl Coenzyme A Reductase, on Cholesterol Gallstone Formation in Prairie Dogs

Digestion (1992)

Susumu Tazuma S Hatsushika Naoki Aihara Hiroshi Sagawa Y Gunji

The effects of pravastatin on cholesterol gallstone formation were determined in prairie dogs. We fed 10 prairie dogs 1 % cholesterol with or without 0.05% (w/w) pravastatin (n = 5, each) for 4 weeks. In addition, another 5 prairie dogs were fed a standard rodent chow as a control. Only the animals fed 1 % cholesterol without pravastatin treatment formed cholesterol gallstones. Gallbladder bile from cholesterol-fed animals contained cholesterol monohydrate crystals, whereas those treated with pravastatin contained no crystal. Furthermore, marked increases in tissue cholesterol levels (serum, liver and bile), and in biliary mucous glycoprotein levels were evident in cholesterol-fed animals, whereas pravastatin treatment normalized these levels. These findings raise the possibility that such inhibitors might have a future role to play in the prevention of cholesterol gallstone formation and/or recurrence.

Hydroxymethylglutaryl-CoA reductase

Reverse cholesterol transport

Cholesterol-lowering

10.1159/000200894

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Citations (22)

Partial characterization of the mechanism(s) whereby apolipoprotein A-1 inhibits cholesterol crystal nucleation in the supersaturated model bile systems.

Kanzo (1991)

Seishi Tao Susumu Tazuma Shigeki Mizuno H Sasaki Hiroshi Sagawa

コレステロール過飽和人工胆汁を用いて,アポ蛋白A1のコレステロール結晶析出抑制の作用機序を検討した.アポ蛋白A1を添加した人工胆汁(A1+群)と無添加人工胆汁(A1-群)の脂質粒子をカラムクロマトグラフィー法にて分画採取し,両群の脂質粒子の形態変化を透過型電子顕微鏡にて経時的に比較検討した.両群において胆汁中脂質粒子は,ミセル分画と非ミセル分画に分離でき,A1+群においてアポ蛋白A1は,ミセル分画のピークよりわずかに高分子量領域にピークを有して溶出された.A1+, A1-両群において,同分画を透過型電子顕微鏡で観察すると,直径200～400Åのdisc様粒子が認められ,A1-群では,粒子の迅速な凝集と融合が観察されたのに対し,A1+群では,その変化は極めて緩徐であった.以上,コレステロール過飽和人工胆汁において,アポ蛋白A1のdisc様粒子安定化による,コレステロール結晶析出の抑制が示唆された.

Supersaturation

Characterization

Crystal (programming language)

10.2957/kanzo.32.1132

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Citations (0)

A novel method for simultaneous quantititation of vesicle aggregation and fusion: Relation to biliary lipid secretion and bile metastability

Gastroenterology (1995)

Hiroki Ochi Susumu Tazuma T Ohya Yuya Hattori K Horikawa

Metastability

Vesicle fusion

10.1016/0016-5085(95)28844-9