David Piñeyro

Centro de Investigación Biomédica en Red de Cáncer

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Manel Esteller

Institució Catalana de Recerca i Estudis Avançats

Manuel Castro de Moura

Josep Carreras Leukaemia Research Institute

Arcadi Navarro

Centre for Genomic Regulation

Carolina Minguillón

Pasqual Maragall Foundation

Juan Domingo Gispert

Pasqual Maragall Foundation

Natàlia Vilor‐Tejedor

Centre for Genomic Regulation

José Luís Molinuevo

Pasqual Maragall Foundation

Grégory Operto

Pasqual Maragall Foundation

Karine Fauria

Pasqual Maragall Foundation

Alberto Villanueva

Institut d'Investigació Biomédica de Bellvitge

Cooperative Institutions

Institut d'Investigació Biomédica de Bellvitge

Universitat de Barcelona

Instituto de Salud Carlos III

Universitat Autònoma de Barcelona

Centro de Investigación Biomédica en Red

Institut Català d'Oncologia

Josep Carreras Leukaemia Research Institute

Bellvitge University Hospital

Pompeu Fabra University

Pasqual Maragall Foundation

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

HeT-A_pi1, a piRNA Target Sequence in the Drosophila Telomeric Retrotransposon HeT-A, Is Extremely Conserved across Copies and Species

PLoS ONE (2012)

Natalia Petit David Piñeyro Elisenda López-Panadès Elena Casacuberta Arcadi Navarro

The maintenance of the telomeres in Drosophila species depends on the transposition of the non-LTR retrotransposons HeT-A, TAHRE and TART. HeT-A and TART elements have been found in all studied species of Drosophila suggesting that their function has been maintained for more than 60 million years. Of the three elements, HeT-A is by far the main component of D. melanogaster telomeres and, unexpectedly for an element with an essential role in telomere elongation, the conservation of the nucleotide sequence of HeT-A is very low. In order to better understand the function of this telomeric retrotransposon, we studied the degree of conservation along HeT-A copies. We identified a small sequence within the 3′ UTR of the element that is extremely conserved among copies of the element both, within D. melanogaster and related species from the melanogaster group. The sequence corresponds to a piRNA target in D. melanogaster that we named HeT-A_pi1. Comparison with piRNA target sequences from other Drosophila retrotransposons showed that HeT-A_pi1 is the piRNA target in the Drosophila genome with the highest degree of conservation among species from the melanogaster group. The high conservation of this piRNA target in contrast with the surrounding sequence, suggests an important function of the HeT-A_pi1 sequence in the co-evolution of the HeT-A retrotransposon and the Drosophila genome.

Retrotransposon

Piwi-interacting RNA

Conserved sequence

Melanogaster

10.1371/journal.pone.0037405

Cite

Citations (3)

Biogenesis and Evolution of Functional tRNAs

Springer eBooks (2014)

David Piñeyro Adrian Gabriel Torres Lluı́s Ribas de Pouplana

Genetic Code

10.1007/978-3-319-05687-6_10

Cite

Citations (13)

Genetically predicted telomere length and Alzheimer’s disease endophenotypes: a Mendelian randomization study

Alzheimer s Research & Therapy (2022)

Blanca Rodríguez‐Fernández Natàlia Vilor‐Tejedor Eider M. Arenaza‐Urquijo Gonzalo Sánchez‐Benavides Marc Suárez‐Calvet

Abstract Telomere length (TL) is associated with biological aging, consequently influencing the risk of age-related diseases such as Alzheimer’s disease (AD). We aimed to evaluate the potential causal role of TL in AD endophenotypes (i.e . , cognitive performance, N = 2233; brain age and AD-related signatures, N = 1134; and cerebrospinal fluid biomarkers (CSF) of AD and neurodegeneration, N = 304) through a Mendelian randomization (MR) analysis. Our analysis was conducted in the context of the ALFA (ALzheimer and FAmilies) study, a population of cognitively healthy individuals at risk of AD. A total of 20 single nucleotide polymorphisms associated with TL were used to determine the effect of TL on AD endophenotypes. Analyses were adjusted by age, sex, and years of education. Stratified analyses by APOE -ɛ4 status and polygenic risk score of AD were conducted. MR analysis revealed significant associations between genetically predicted longer TL and lower levels of CSF Aβ and higher levels of CSF NfL only in APOE -ɛ4 non-carriers. Moreover, inheriting longer TL was associated with greater cortical thickness in age and AD-related brain signatures and lower levels of CSF p-tau among individuals at a high genetic predisposition to AD. Further observational analyses are warranted to better understand these associations. Graphical Abstract

Mendelian Randomization

Endophenotype

Genetic predisposition

10.1186/s13195-022-01101-9

Cite

Citations (21)

Transcriptional analysis of the HeT-A retrotransposon in mutant and wild type stocks reveals high sequence variability at Drosophila telomeres and other unusual features

BMC Genomics (2011)

David Piñeyro Elisenda López-Panadès María Lucena-Pérez Elena Casacuberta

Abstract Background Telomere replication in Drosophila depends on the transposition of a domesticated retroelement, the HeT-A retrotransposon. The sequence of the HeT-A retrotransposon changes rapidly resulting in differentiated subfamilies. This pattern of sequence change contrasts with the essential function with which the HeT-A is entrusted and brings about questions concerning the extent of sequence variability, the telomere contribution of different subfamilies, and whether wild type and mutant Drosophila stocks show different HeT-A scenarios. Results A detailed study on the variability of HeT-A reveals that both the level of variability and the number of subfamilies are higher than previously reported. Comparisons between GIII, a strain with longer telomeres, and its parental strain Oregon-R indicate that both strains have the same set of HeT-A subfamilies. Finally, the presence of a highly conserved splicing pattern only in its antisense transcripts indicates a putative regulatory, functional or structural role for the HeT-A RNA. Interestingly, our results also suggest that most HeT-A copies are actively expressed regardless of which telomere and where in the telomere they are located. Conclusions Our study demonstrates how the HeT-A sequence changes much faster than previously reported resulting in at least nine different subfamilies most of which could actively contribute to telomere extension in Drosophila. Interestingly, the only significant difference observed between Oregon-R and GIII resides in the nature and proportion of the antisense transcripts, suggesting a possible mechanism that would in part explain the longer telomeres of the GIII stock.

Retrotransposon

Retroposon

10.1186/1471-2164-12-573

Cite

Citations (14)

The transcribed pseudogene RPSAP52 enhances the oncofetal HMGA2-IGF2BP2-RAS axis through LIN28B-dependent and independent inhibition of let-7 miRNAs

Cristina Oliveira-Mateos Anaís Sánchez‐Castillo Marta Soler Aida Obiols-Guardia David Piñeyro

Pseudogene

Source

Cite

Citations (0)

Gene Amplification-Associated Overexpression of the Selenoprotein tRNA Enzyme TRIT1 Confers Sensitivity to Arsenic Trioxide in Small-Cell Lung Cancer

Cancers (2021)

Laia Coll-SanMartin Verónica Dávalos David Piñeyro Margalida Rosselló-Tortella Alberto Bueno-Costa

The alteration of RNA modification patterns is emerging as a common feature of human malignancies. If these changes affect key RNA molecules for mRNA translation, such as transfer RNA, they can have important consequences for cell transformation. TRIT1 is the enzyme responsible for the hypermodification of adenosine 37 in the anticodon region of human tRNAs containing serine and selenocysteine. Herein, we show that TRIT1 undergoes gene amplification-associated overexpression in cancer cell lines and primary samples of small-cell lung cancer. From growth and functional standpoints, the induced depletion of TRIT1 expression in amplified cells reduces their tumorigenic potential and downregulates the selenoprotein transcripts. We observed that TRIT1-amplified cells are sensitive to arsenic trioxide, a compound that regulates selenoproteins, whereas reduction of TRIT1 levels confers loss of sensitivity to the drug. Overall, our results indicate a role for TRIT1 as a small-cell lung cancer-relevant gene that, when undergoing gene amplification-associated activation, can be targeted with the differentiation agent arsenic trioxide.

Arsenic Trioxide

Selenocysteine

Selenoprotein

10.3390/cancers13081869

Cite

Citations (10)

Repression by TTK69 of GAGA-mediated Activation Occurs in the Absence of TTK69 Binding to DNA and Solely Requires the Contribution of the POZ/BTB Domain of TTK69

Journal of Biological Chemistry (2004)

Sara Pagans David Piñeyro Ana Kosoy Jordi Bernués Fernando Azorı́n

tramtrack 69 (TTK69) is known to repress GAGA-mediated activation of the eve promoter in S2 cells. Here, we show that repression by TTK69 occurs in the absence of bona fide TTK69-binding sites on the template, indicating that it does not require the binding of TTK69 to DNA. Consistent with this interpretation, the POZ/BTB domain of TTK69, which does not bind DNA, is sufficient for repression. Moreover, a fusion protein in which the POZ/BTB domain of GAGA is replaced by that of TTK69 is not capable of activating the eve promoter but efficiently represses GAGA-dependent activation. Repression involves GAGA-TTK69 interaction because TTK69 is not capable of repressing basal transcription. Most probably, GAGA-TTK69 interaction occurs at the promoter because GAGA·TTK69 complexes are fully competent in binding DNA in vitro. Our results also show that repression by TTK69 of GAGA-dependent activation of the eve promoter is not mediated by any of the co-repressors known to interact with TTK69 (dMi2 or C-terminal binding protein) or by trichostatin A-sensitive histone deacetylases. Altogether, these observations strongly suggest that the binding of TTK69 prevents the interaction of GAGA with the transcription machinery and, therefore, compromises its activation potential.

Transcription

10.1074/jbc.m313200200

Cite

Citations (21)

Characterization of Acute HCV Infection and Transmission Networks in People Who Currently Inject Drugs in Catalonia: Usefulness of Dried Blood Spots

Hepatology (2021)

Adrián Antuori Vincent Montoya David Piñeyro Lauro Sumoy Jeffrey B. Joy

Background and Aims Accurate identification of recent HCV infections is critical for tracing the extent and mechanisms of ongoing transmission. We aimed to validate dried blood spot (DBS) samples for the assessment of Hepatitis C virus (HCV) genetic diversity and to determine epidemiological parameters including incidence, determinants of acute infection, and phylogenetic clustering in people who inject drugs (PWID). Approach and Results HCV nonstructural protein 5B next‐generation sequencing was performed from plasma and/or DBS in 220 viremic PWID from the HepC detect II study. No significant differences were found in consensus sequences or Shannon entropy (SE) intrahost diversity estimate between paired plasma/DBS specimens. SE values were used to identify acute infections with 93.3% sensitivity (95% CI, 0.81–1.06) and 95.0% specificity (95% CI, 0.88–1.02) in a set of well‐defined controls. An acute HCV infection (either primary infection or reinfection) was detected in 13.5% of viremic participants and was associated with age ≤30 years (OR, 8.09), injecting less than daily (OR, 4.35), ≤5 years of injected drug use (OR, 3.43), sharing cocaine snorting straws (OR, 2.89), and being unaware of their HCV status (OR, 3.62). Annualized HCV incidence was estimated between 31 and 59/100 person‐years. On phylogenetic analysis, 46.8% of viremic cases were part of a transmission pair or cluster; age ≤30 years (OR, 6.16), acute infection (OR, 5.73), and infection with subtype 1a (OR, 4.78) were independently associated with this condition. Conclusions The results obtained from plasma and DBS characterize PWID with acute infection and those involved in ongoing HCV transmission and allow estimating incidence from cross‐sectional data. This information is critical for the design and assessment of targeted harm reduction programs and test‐and‐treat interventions and to facilitate monitoring of HCV elimination in this key population.

Dried blood

Spots

10.1002/hep.31757

Cite

Citations (5)

New insights forDrosophilaGAGA factor in larvae

Royal Society Open Science (2015)

Marta Blanch David Piñeyro Jordi Bernués

GAGA factor plays important roles during Drosophila embryogenesis and its maternal contribution is essential for early development. Here, the role of GAGA factor was studied in 3rd instar larvae using depletion and overexpression conditions in wing disc and transcriptome analysis. We found that genes changing expression were different to those previously described using GAGA mutants in embryos. No apparent phenotypes on GAGA depletion could usually be observed at larval stages in imaginal discs but a strong effect on salivary gland polytene chromosomes was observed. In the adult, GAGA depletion produced many defects like abnormal cell proliferation in the wing, impaired dorsal closure and resulted in homeotic transformation of abdominal segment A5. Unexpectedly, no effects on Ultrabithorax expression were observed. Short overexpression of GAGA factor in 3rd instar larvae also resulted in activation of a set of genes not previously described to be under GAGA regulation, and in lethality at pupa. Our results suggest a little contribution of GAGA factor on gene transcription in wing discs and a change of the genes regulated in comparison with embryo. GAGA factor activity thus correlates with the global changes in gene expression that take place at the embryo-to-larva and, later, at the larva-to-pupa transitions.

Polytene chromosome

10.1098/rsos.150011

Cite

Citations (7)

Molecular Features of Cancers Exhibiting Exceptional Responses to Treatment

Cancer Cell (2020)

David A. Wheeler Naoko Takebe Toshinori Hinoue Katherine A. Hoadley Maria Cardenas

10.1016/j.ccell.2020.10.015

Cite

Citations (85)