M. Elisabete C.D. Real Oliveira

University of Minho

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Madalena Lira

University of Minho

Marlene Lúcio

University of Minho

Elisabete M. S. Castanheira

University of Minho

Andreia C. Gomes

University of Minho

Joana Azeredo

University of Minho

Lívia Santos

Nottingham Trent University

Eva Yebra‐Pimentel

Universidade de Santiago de Compostela

Paulo J. G. Coutinho

University of Minho

A. C. Oliveira

Centro Universitário das Faculdades Metropolitanas Unidas

Rosário Oliveira

University of Minho

Cooperative Institutions

University of Minho

117

Universidade do Porto

Clinical Academic Center of Braga

Universidade de Santiago de Compostela

Rede de Química e Tecnologia

University of Cuenca

University of Coimbra

Institute for Biotechnology and Bioengineering

Moscow Engineering Physics Institute

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto

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Evaluation of optical properties of different types of contact lenses

Clara Pereira Elisabete M. S. Castanheira M. Elisabete C.D. Real Oliveira Madalena Lira

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Adhesion of Pseudomonas aeruginosa and Staphylococcus epidermidis to Silicone???Hydrogel Contact Lenses

Optometry and Vision Science (2005)

Mariana Henriques Cláudia Sousa Madalena Lira M. Elisabete M. Elisabete C.D. Real Oliveira

Purpose. The purpose of this study is to compare the adhesion capabilities of the most important etiologic agents of microbial ocular infection to the recently available silicone–hydrogel lenses with those to a conventional hydrogel lens. Methods. In vitro static adhesion assays of Pseudomonas aeruginosa 10,145, Staphylococcus epidermidis 9142 (biofilm-positive), and 12,228 (biofilm-negative) to two extended-wear silicone–hydrogel lenses (balafilcon A and lotrafilcon A), a daily wear silicone–hydrogel lens (galyfilcon A) and a conventional hydrogel (etafilcon A) were performed. To interpret the adhesion results, lens surface relative hydrophobicity was assessed by water contact angle measurements. Results. P. aeruginosa and S. epidermidis 9142 exhibited greater adhesion capabilities to the extended wear silicone–hydrogel lenses than to the daily wear silicone– and conventional hydrogel lenses (p < 0.05). No statistical differences were found between the adhesion extent of these strains to galyfilcon A and etafilcon A. The biofilm negative strain of S. epidermidis adhered in larger extents to the silicone–hydrogel lenses than to the conventional hydrogel (p < 0.05), but in much lower amounts than the biofilm-positive strain. The water contact angle measurements revealed that the extended wear silicone–hydrogel lenses are hydrophobic, whereas the daily wear silicone– and conventional hydrogel lenses are hydrophilic. Conclusions. As a result of their hydrophobicity, the extended wear silicone–hydrogel lenses (lotrafilcon A and balafilcon A) may carry higher risk of microbial contamination than both the hydrophilic daily wear silicone–hydrogel lens, galyfilcon A and the conventional hydrogel lens, etafilcon A.

Silicone hydrogel

Silicone Elastomers

10.1097/01.opx.0000168585.53845.64

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The nanotherapy revolution: three different applications of lipid nanosystems in therapeutics

Journal of Bioengineering and Biomedical Sciences (2016)

M. Elisabete C.D. Real Oliveira Marlene Lúcio Telma Soares Ennia Rodrigues Fernandes Josivaldo J G da Silva

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The potential use of biosurfactants as contact lenses coating agents

Essen Symposium (2009)

Verónica Rocha Eduardo J. Gudiña Madalena Lira Elisabete M. S. Castanheira M. Elisabete C.D. Real Oliveira

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Novel strategies for cancer treatment: drug delivery combining graphene quantum dots and core-filled lipid vesicles

Rita Mendes Telma Soares Eduarda Fernandes Hugo Gonçalves L. Alves

This work was supported by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013. Marlene Lucio acknowledges the exploratory project funded by FCT with the reference IF/00498/2012. Eduarda Fernandes and Telma Soares acknowledge COMPETE 2020 “Programa Operacional Competitividade e internacionalizacao”.

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Contact Lens Hydrophobicity and Roughness Effects on Bacterial Adhesion

Optometry and Vision Science (2010)

María J. Giráldez Carlos García‐Resúa Madalena Lira M. Elisabete C.D. Real Oliveira Beatríz Magariños

This study was designed to assess whether hydrogel contact lens (CL) surface hydrophobicity and roughness affect Staphylococcus epidermidis adhesion.Bacterial adhesion experiments were performed on two unworn silicone hydrogel and three unworn conventional hydrogel CLs using the S.epidermidis strain CECT 4184. Microbial colonization was assessed by conducting counts expressed as colony-forming units. CL hydrophobicity was determined through water contact angle measurements and the roughness parameters such as mean surface roughness (Ra), kurtosis (Rku), and skewness (Rsk) were determined through atomic force microscopy in Tapping Mode.The conventional CLs showed similar water contact angles (p > 0.05) and were classified as hydrophilic. The silicone hydrogel CLs yielded hydrophobic contact angles with no significant differences between them (p > 0.05). The lenses with the highest (nelfilcon A and ocufilcon B) or lowest (comfilcon A and omafilcon A) Ra values displayed a lesser or greater extent of spikiness of their surfaces, respectively. All lenses showed a predominance of peaks (Rsk > 0) over troughs. S. epidermidis adhered more to the hydrophobic CLs (p < 0.05). Omafilcon A and comfilcon A, which showed the lowest Ra values among the hydrophilic and hydrophobic lenses, respectively, returned the lowest bacterial adhesion scores (p < 0.05).Our results suggest that more hydrophobic CLs are more prone to S. epidermidis adhesion. Although the Ra appears to be related to S. epidermidis adhesion, the influence of Rku and Rsk on this variable remains unclear.

CLs upper limits

10.1097/opx.0b013e3181da8656

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Drug biophysical profiling using lipid-based colloidal nanosystems and human serum albumin as biomimetic interfaces

Eduarda Fernandes M. Elisabete C.D. Real Oliveira Sofia Benfeito Fernando Cagide Fernanda Borges

This work was supported by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013. We also acknowledge PEstC/QUI/UI0081/2013, NORTE-01-0145-FEDER-000028 and PTDC/DTP-FTO/2433/2014. F. Cagide and S. Benfeito are thankful for the pos-doctoral and doctoral grants (SFRH/BPD/74491/2010 and SFRH/BD/99189/2013 respectively). Marlene Lucio acknowledges the exploratory project funded by FCT with the reference IF/00498/2012. Eduarda Fernandes acknowledges COMPETE 2020 “Programa Operacional Competitividade e internacionalizacao”.

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How Multi-Step versus One-Step Preparation Method Affects the Physicochemical Properties and Transfection Efficiency of DNA/DODAB:MO Lipoplexes

Journal of Applied Solution Chemistry and Modeling (2014)

M. Elisabete C.D. Real Oliveira João Neves Silva A. C. Oliveira Marlene Lúcio Andreia C. Gomes

The consequences for the transfection efficiencies of different lipoplexes preparation methods, largely remain to be explored, but the knowledge of how different experimental approaches can affect the physicochemical properties and transfection efficiency is essential for a proper tailoring of transfection complexes to particular applications. Therefore, the influence of the number of mixing steps (one-step addition versus multi-step addition of liposomes to plasmid DNA (pDNA)) and lipoplex incubation temperature on the final physicochemical properties and transfection efficiency of pDNA/ Dioctadecyldimethylammonium Bromide (DODAB):1-monooleoyl-rac-glycerol (MO) complexes was studied in three distinct DODAB:MO molar ratios: 4:1, 2:1 and 1:1. Dynamic Light Scattering (DLS), Zeta (ζ) Potential, Ethidium Bromide (EtBr) exclusion assays were used to assess the formation, structure and destabilization of the lipoplexes, whereas in vitro transfection assays with pSV-β-gal plasmid DNA were performed to evaluate their transfection efficiency on the 293T mammalian cell line. Results indicate that the morphology of pDNA/DODAB:MO complexes is dependent on the lipoplex preparation method, resulting in particles of distinct size, surface charge and membrane fluidity. These variations are visible during the complexation dynamics of pDNA and continue throughout the profile of pDNA release from pDNA/DODAB:MO lipoplexes upon incubation with Heparin (HEP), as well as in the in vitro transfection assays. The stepwise addition of DODAB:MO vesicles to pDNA decreases the transfection efficiency of the lipoplexes, while the effect of the lipoplex preparation methods is dependent on the MO content.

One-Step

Two step

10.6000/1929-5030.2014.03.02.7

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Surface Roughness and Refractive Index Changes in Contact Lens Induced by Lens Care Systems

Eye & Contact Lens Science & Clinical Practice (2014)

Madalena Lira Sandra Franco José Benito Vázquez Dorrío M. Elisabete C.D. Real Oliveira Manuel F. M. Costa

The aim of this study was to analyze the influence of different lens care systems in surface roughness and refractive index (RI) of contact lenses (CL). This information provides us with a better understanding of how care solutions affect CL materials.Several CL and three commercially available and appropriate lens care solutions were used (two polyhexamethylene biguanide and one hydrogen peroxide care systems). Lenses were immersed in the lens care systems, and then measurements with CLR 12-70 digital automated refractometer and atomic force microscopy analysis on Tapping mode were recorded. The measurements were performed before and after the lenses were immersed in each care solution.Significant changes were observed on the CL materials when exposed to lens care systems. All the materials changed, to a greater or lesser extent, their roughness and RI, after being immersed in the different solutions. The water content varied between 0% (Nelfilcon A in ReNu Multiplus, Senofilcon A in AOSEPT Plus, and Methafilcon A in Solocare Aqua) and 4.1% (Hilafilcon B in Solocare Aqua) The higher change in roughness was obtained with ReNu Multiplus in the lens Comfilcon A (with an increase of 27.2 nm) and Senofilcon A (with an increase of 16.7 nm).This study suggests that lens care systems play an important role in surface roughness and RI of CL.

10.1097/icl.0000000000000023

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Caracterização qualitativa e quantitativa de depósitos de proteínas em lentes de contacto de silicone-hidrogel

Lívia Santos Madalena Lira Diana Rodrigues Rosário Oliveira M. Elisabete C.D. Real Oliveira

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