An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/cc128s15

Cite

Citations (0)

Ex vivo expansion of alveolar macrophages with Mycobacterium tuberculosis from the resected lungs of patients with pulmonary tuberculosis

PLoS ONE (2018)

Elena Ufimtseva Natalya Eremeeva Ekaterina Petrunina Tatiana Umpeleva Svetlana Karskanova

Tuberculosis (TB), with the Mycobacterium tuberculosis (Mtb) as the causative agent, remains to be a serious world health problem. Traditional methods used for the study of Mtb in the lungs of TB patients do not provide information about the number and functional status of Mtb, especially if Mtb are located in alveolar macrophages. We have developed a technique to produce ex vivo cultures of cells from different parts of lung tissues surgically removed from patients with pulmonary TB and compared data on the number of cells with Mtb inferred by the proposed technique to the results of bacteriological and histological analyses used for examination of the resected lungs. The ex vivo cultures of cells obtained from the resected lungs of all patients were largely composed of CD14-positive alveolar macrophages, foamy or not, with or without Mtb. Lymphocytes, fibroblasts, neutrophils, and multinucleate Langhans giant cells were also observed. We found alveolar macrophages with Mtb in the ex vivo cultures of cells from the resected lungs of even those TB patients, whose sputum smears and lung tissues did not contain acid-fast Mtb or reveal growing Mtb colonies on dense medium. The detection of alveolar macrophages with Mtb in ex vivo culture as soon as 16–18 h after isolation of cells from the resected lungs of all TB patients suggests that the technique proposed for assessing the level of infection in alveolar macrophages of TB patients has higher sensitivity than do prolonged bacteriological or pathomorphological methods. The proposed technique allowed us to rapidly (in two days after surgery) determine the level of infection with Mtb in the cells of the resected lungs of TB patients and, by the presence or absence of Mtb colonies, including those with cording morphology, the functional status of the TB agent at the time of surgery.

Ex vivo

Alveolar macrophage

10.1371/journal.pone.0191918

Cite

Citations (16)

Mycobacterium tuberculosis Cording in Alveolar Macrophages of Tuberculosis Patients and Mycobacterial Virulence

Tuberculosis (2017)

Elena Ufimtseva Natalya Eremeeva М. А. Кравченко Diana Vakhrusheva Sergey Skornyakov

10.1183/1393003.congress-2017.pa2749

Cite

Citations (0)

Polycyclotrimerization of aryldicyanates in the presence of Cr3+ acetylacetonate

Polymer Science U S S R (1980)

A.K. Bonetskaya V.V. Ivanov М. А. Кравченко В. А. Панкратов Ts. M. Frenkel'

Kinetic scheme

Propane

Basis (linear algebra)

10.1016/0032-3950(80)90275-0

Cite

Citations (11)

Effect of the nature of solvent on the polycyclotrimerization rate of 2,2-bis-(4-Cyanatophenyl) propane

Polymer Science U S S R (1977)

A.K. Bonetskaya М. А. Кравченко Ts. M. Frenkel'В. А. Панкратов S.V. Vinogradova

Diglyme

Anisole

Nitrobenzene

Propane

10.1016/0032-3950(77)90043-0

Cite

Citations (11)

Synthesis andin vitro tuberculostatic activity of podands containing semicarbazide or thiosemicarbazide fragments

Pharmaceutical Chemistry Journal (1998)

О. В. Федорова Г. Г. Мордовской Gennady L. Rusinov I.G. Ovchinnikova М. Н. Зуева

Semicarbazide

10.1007/bf02464163

Cite

Citations (7)

The problem of choosing disinfecting agents to decontaminate the surfaces of objects at a tuberculosis hospital

DOAJ (DOAJ: Directory of Open Access Journals) (2015)

Н. И. Еремеева V. V. Kanishchev М. А. Кравченко Diana Vakhrusheva

В противотуберкулезных учреждениях сконцентрированы наиболее эпидемически опасные пациенты – больные туберкулезом легких, которые выделяют в окружающую среду большое количество микобактерий туберкулеза (МБТ): в суточном количестве мокроты больного туберкулезом может находиться свыше 7 млрд МБТ [16]. При отсутствии эффективной вентиляции после выделения из макроорганизма микобактерии в течение нескольких часов могут находиться в воздухе в виде частиц аэрозоля, оседающих затем на поверхности различных объектов, на которых могут накапливаться в значительном количестве, превышающем минимальную дозу заражения [5, 38, 49]. Длительный контакт восприимчивого к этой инфекции человека с объектами (посудой, одеждой, мебелью и другими предметами и поверхностями), загрязненными патогенными микобактериями, может привести к заболеванию туберкулезом [32, 33, 38, 52]. Усугубляет эпидемическую ситуацию высокий уровень распространения в РФ возбудителя туберкулеза с множественной (МЛУ) и широкой лекарственной устойчивостью [41, 48]. Таким образом, в противотуберкулезных учреждениях сотрудники, пациенты и посетители подвергаются риску заражения нозокомиальным туберкулезом [7, 8, 20, 28, 31, 38].

10.21292/2075-1230-2015-0-2-12-19

Cite

Citations (0)

Fluoroquinolones with Tuberculostatic Activity.

ChemInform (2005)

G. N. Lipunova Emiliya V. Nosova М. А. Кравченко Nataliya N. Mochulskaya Л. П. Сидорова

10.1002/chin.200518134

Cite

Citations (1)