Lorenzo Segabinazzi

Ross University School of Veterinary Medicine

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Marco Antônio Alvarenga

Universidade Estadual Paulista (Unesp)

Igor F. Canisso

Universidade Estadual Paulista (Unesp)

Frederico Ozanam Papa

Universidade Estadual Paulista (Unesp)

J.A. Dell’Aqua

Universidade Estadual Paulista (Unesp)

Camila de Paula Freitas‐Dell’Aqua

Universidade Estadual Paulista (Unesp)

Hilari French

Ross University School of Veterinary Medicine

Fernando Paixão

Universidade Estadual Paulista (Unesp)

Sidnei Nunes de Oliveira

Universidade Estadual Paulista (Unesp)

R.O. Gilbert

Ross University School of Veterinary Medicine

André Maciel Crespilho

Universidade de Santo Amaro

Cooperative Institutions

Universidade Estadual Paulista (Unesp)

Universidade de São Paulo

Ross University School of Veterinary Medicine

Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia

University of Illinois Urbana-Champaign

Universidade Federal de Pelotas

Universidade de Santo Amaro

Universidade Federal da Bahia

Medecell (Brazil)

University of California, Davis

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Semen parameters and embryo recovery of cooled stallion semen extended with sodium caseinate-cholesterol (Botugold) and phosphocaseinate (INRA96) based extenders

Journal of Equine Veterinary Science (2020)

Guilherme Novello Giorgia Podico Lorenzo Segabinazzi Igor F. Canisso

Semen cryopreservation

Semen extender

Capacitation

Membrane integrity

10.1016/j.jevs.2020.103043

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Citations (0)

Antimicrobial activity of alternative therapies for microorganisms causing chronic endometritis in mares

Journal of Equine Veterinary Science (2023)

Mariana P. Mazzuchini Lorenzo Segabinazzi Igor F. Canisso

10.1016/j.jevs.2023.104730

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Citations (2)

Paradoxical Effect of Quercetin Antioxidant on Goat Sperm Parameters After Cryopreservation.

Cryoletters (2020)

Marcelo Sant’Ana Borges Joana Larissa Barbosa Born Laura Conti Lorenzo Segabinazzi M. Nichi

BACKGROUND Some antioxidants have been used in semen extenders to reduce adverse effects caused by excessive reactive oxygen species (ROS) production. The study was carried out to assess the effect of quercetin (QC) antioxidant therapy on goat semen submitted to cryopreservation. OBJECTIVE To evaluate the effect of quercetin incorporation in different phases of the cryopreservation process of goat spermatozoa. MATERIALS AND METHODS Five ejaculates from each of four goats (n= 20) were collected and split into four groups: Control (G1), without QC; G2, 15 μM of QC added to semen before centrifugation; G3, 15 μM QC added to semen after centrifugation; G4, 15 μM QC added to semen before centrifugation and 15 μM of QC added to semen after centrifugation (total of 30 μM of QC); and cryopreserved. All semen samples were evaluated after thawing for sperm kinetics, plasma membrane integrity, and ROS levels. RESULTS Although lower concentrations of ROS were associated with groups that received antioxidant supplementation (P=0.0213), linear and dose dependent (P<0.05) reductions of the total and progressive sperm motility, velocity and percentage of fast cells were related to the QC groups. Likewise, plasma membrane integrity was better preserved (P=0.0154) in the control group (35.5%) than in groups that received QC (G2=32.6%, G3=32.4% and G4=26.7%). CONCLUSION Although quercetin was efficient at reducing the oxidative stress related to sperm cryopreservation, it exerted a deleterious dose-dependent effect on the kinetics and integrity of the frozen goat semen, contradicating its use in the tested concentrations.

Semen cryopreservation

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Citations (3)

•Como manejar doenças metabólicas que interferem na eficiência reprodutiva em éguas (

REVISTA BRASILEIRA DE MEDICINA VETERINÁRIA EQUINA (2020)

Fernando Paixão Mariana Polesso Mazzuchini Guilherme Novello Lorenzo Segabinazzi

10.29327/236004.14.87-2

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Uterine Inflammatory Response After Prostaglandin E1 (Misoprostol) Infusion Prebreeding or Immediately After Embryo Flushing in Commercial Donor Mares

Journal of Equine Veterinary Science (2022)

G Amorim Lorenzo Segabinazzi Odilon Marquez de Oliveira Simone Perecmanis Rodrigo Arruda de Oliveira

Flushing

Uterine horns

10.1016/j.jevs.2022.104060

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Citations (2)

Morphometric changes in the hoof capsule of Criollo foals from birth to weaning

Ciência Rural (2017)

João Ricardo Malheiros de Souza Anelise Maria Hammes Pimentel Vinícius Azevedo Folle J. Pfeifer Aline Bacchieri Gallo Schuster

ABSTRACT: South America has numerous Criollo horse breeding farms; however, information on foal hoof growth is still limited and identifying the ideal periods to apply corrective trimming is a frequent concern for horse owners. In the present study, a morphometric analysis of hoof growth was performed on 46 Criollo foals from birth to weaning (0-8 months). Results showed that hoof growth rate was higher in the first four months followed by a decrease until the eighth month. Average growth rate of the hoof was 0.21cm per month, whereas that of the heel was 0.14cm per month. However, no significant differences were observed between medial and lateral heel length or between limbs. Coronary band perimeter and solar width showed an average increase of 0.97cm and 0.46cm per month, respectively, and were significantly correlated (r=0.99, P≤0.01). The characteristic most positively correlated to biometric variables was foal age, followed by solar width, toe length, and coronary band perimeter. In conclusion, hoof length increase in Criollo foals was more intensive during the first four months of life.

Hoof

Foal

Perimeter

Pony

10.1590/0103-8478cr20160945

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Citations (5)

Comparison of blood flow on testicular artery, hormone dosage and semen quality between texel and “Santa Ines” sheep

Animal Reproduction (2015)

Y.F.R. Sancler-Silva Lorenzo Segabinazzi R. A. Schimith C. Ramires Neto Gabriele Barros Mothé

The aims of this study were to evaluate and compare testicular parameters, sperm quality and hormonal dosage between Texel and Santa Ines sheep. For this, 25 healthy animals (12 Texel and 13 Santa Ines) aged between 2 to 4 years old were used, from the Department of Animal Reproduction and Veterinary Radiology of Sao Paulo State University (UNESP – Botucatu). Testicular morphometry was initially performed (length - LEN and width - WID in centimeters) and then the spectral doppler ultrasound examination of testicular artery (pulsatility index - PI and resistance index - RI) was evaluated in the spermatic cord. Subsequently a sample of blood from each animal was collected in the morning by puncture of the external jugular vein were collected for access the testosterone dosage (DT, mg/dL) and then the semen was collected by electroejaculation. The sperm kinetic parameters were analyzed by computerized method CASA (TM% total motility, %PM - progressive motility, µm/s VAP – average speed path, µm/s VCL - curvilinear velocity, µm/s VSL - progressive linear speed, %RAP - rapid sperm) and the plasma membrane integrity (MPI) was analyzed by epifluorescence microscopy. The testosterone dosage was access by kits and the analysis performed by radioimmunoassay. The data generated were evaluated by Student´s t-test and differences were considered significant at p<0.05. The mean values and standard deviations found for the evaluated parameters for Texel and Santa Ines breeds are placed following, respectively: TM (81±7.4 and 84±17.8); PM (57.4±10.1 and 62.1±17.4); VAP (130.4±19.6 and 131.6±18.3); VCL (195.2±38.1 and 181.0±22.3); VSL (123.4±29.4 and 111.6±8.9); RAP (70.0±10.2 and 74.4±18.3); MPI (64.8±11.4 and 61.2±12.9); LEN left testicle (9±1.11 and 9.5±1.2); WID left testicle (6.2±0.6 and 6.3±0.5); LEN right testicle (9.1±0.9 and 9.7±1.2); WID right testicle (6.1±0.7 and 6.2±0.5); DT (1.08±0.65 and 2.97 5.6); PI left testicle (0.99±0.27 and 1.16±0.33); RI left testicle (0.63±0.11 and 0.68±0.12); PI right testicle (1.16±0.46 and 1.17±0.47) and RI right testicle (0.67±0.17 and 0.68±0.16). In conclusion there was no difference in any of the parameters evaluated between the sheep breeds.

Electroejaculation

Semen quality

Semen Analysis

Testicular artery

Ductus venosus

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Characterization of sperm traits of donkey semen cryopreserved with different permeable cryoprotectants

Journal of Equine Veterinary Science (2025)

C. Zanardi André Carvalho Camila de Paula Freitas‐Dell’Aqua Marco Antônio Alvarenga F.O. Papa

Cryoprotectant