Autologous bone transplantation known as the "gold standard" to reconstruction of osseous defects has known disadvantages. This study was designed to explore the effects of hydroxy-apatite/tricalcium-phosphate (HA/TCP) and platelet-rich plasma (PRP) on the osteogenesis ability of human adipose-derived mesenchymal stem cells (hAdMSCs) in vitro and in vivo.hAdMSCs were incubated with HA/TCP granules and/or PRP in vitro and then, cell proliferation and differentiation was assessed by MTT assay, AZR S staining and SEM examination. In vivo, four cylindrical defects were drilled in the mandibular bones of 5 mongrel dogs and divided randomly into the following groups: I-autologous crushed bone, II- no filling material, III- HA/TCP and PRP, IV- PRP-enriched hAdMSCs seeded on HA/TCP granules. Inserted hAdMSCs were labeled to trace their contribution to bone tissue regeneration. Finally, cell tracing and tissue regeneration were evaluated by immunohistochemistry and histomorphometry methods, respectively.In vitro, co-incubation with HA/TCP granules significantly reduced proliferation and osteogenic differentiation ability of hAdMSCs; while PRP application promoted these capacities (P<0.05). In vivo, PRP-enriched hAdMSCs seeded on HA/TCP granules induced considerable bone formation in osseous defects (P<0.05). It was obviously shown that hAdMSCs were incorporated into the newly-formed bone.Based on this study, application of stem cells could offer a helpful therapeutic tool in bone tissue regeneration. Although inserted hAdMSCs were identifiable throughout the newly-formed bone tissue, their few number could be an indicator of indirect role of hAdMSCs in tissue regeneration.
This study designed a protocol that would combine pregnant mare serum gonadotrophin (PMSG) and cabergoline (CAB) to induce estrus in bitches. Twenty clinically healthy adult and anestrous female dogs were randomly assigned into four groups. The first group was treated with 5.00 μg kg
Abstract Canine transmissible venereal tumour is a type of neoplasm that can be transmitted through sexual contact in dogs. The occurrence of secondary oral canine transmissible venereal tumour is well documented in dogs; however, primary oral canine transmissible venereal tumour is uncommon. This report presents a rare case of a 2.5‐year‐old, neutered, male Siberian husky dog with an ulcerated mass on the soft palate that was undetected by clinical and radiological examinations, which was later diagnosed as a canine transmissible venereal tumour based on cytology without any genital manifestations. Chemotherapy with vincristine sulfate (5 weeks) resulted in complete recovery of the tumour without recurrence after 6 months. Based on our findings, we emphasise the importance of considering canine transmissible venereal tumour as a possible cause of extragenital masses even in neutered dogs.
Bilateral simple ectopic kidney was diagnosed in an apparently healthy 3-year-old, female domestic short hair cat, incidentally based on radiology and ultrasonography examination. The cat was presented for routine examination without any complaint. In clinical evaluation, no significant abnormal clinical sign was seen except for the absence of both kidneys in their proper location in abdominal palpation, which they were palpated more caudally. Radiography revealed silhouettes of soft tissue opacity in the caudal part of the abdominal cavity superimposed on urinary bladder. Ultrasonography confirmed the presence of both kidneys more caudally. The left kidney was dorsal to the urinary bladder and the right kidney located cranially than the left one. Each kidney showed normal shape and size and imaged in their proper side. Hematological, biochemical and urinalysis examinations showed normal values. Ectopic kidney can be diagnosed in feline patients as an incidental finding but it is important to evaluate the kidney for concurrent problems.
Due to the known disadvantages of autologous bone grafting, tissue engineering approaches have become an attractive method for ridge augmentation in dentistry. To the best of our knowledge, this is the first study conducted to evaluate the potential therapeutic capacity of PRP-assisted hADSCs seeded on HA/TCP granules on regenerative healing response of canine alveolar surgical bone defects. This could offer a great advantage to alternative approaches of bone tissue healing-induced therapies at clinically chair-side procedures.
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