Chien‐Yuan Chen

National Chiayi University

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Ho‐Wen Chen

Tunghai University

Fan-Chieh Yu

National Chung Hsing University

Isao Karube

Tokyo University of Technology

Yen‐Ho Chu

National Chung Cheng University

Yen-Hsun Chuang

National Taiwan University of Science and Technology

Tien-Chien Chen

National Pingtung University of Science and Technology

Yuan‐Chi Su

National Taiwan University

Chin‐Chen Chang

Feng Chia University

Eiichi Tamiya

Osaka Research Institute of Industrial Science and Technology

Su‐Chin Chen

National Chung Hsing University

Cooperative Institutions

National Taiwan University

National Yang Ming Chiao Tung University

National Science and Technology Center for Disaster Reduction

National Chiayi University

National Tsing Hua University

National Defense Medical Center

Tunghai University

National Chung Cheng University

Academia Sinica

National Chung Hsing University

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A common precursor for the three subunits ofL-glutamate oxidase encoded bygoxgene fromStreptomyces platensisNTU3304

Canadian Journal of Microbiology (2001)

Chien‐Yuan Chen Wen-Tung Wu Chang‐Jen Huang Mei‐Huei Lin C. H. Chang

A segment of DNA containing the L-glutamate oxidase (gox) gene from Streptomyces platensis NTU3304 was cloned. The entire nucleotide sequence of the protein-coding portion consisting of 2130 bp (710 codons, including AUG and UGA) of the cloned DNA fragment was determined. The gox gene contained only one open reading frame (ORF) which coded for a 78-kDa polypeptide, the precursor of active extracellular Gox. Mature Gox is composed of three subunits, designated as α, β, and γ, with molecular masses of 39, 19, and 16 kDa, respectively. Analyses of the N-terminal amino acid sequences of the subunits revealed that the order of subunits in the precursor polypeptide encoded by the ORF, from N-terminus to C-terminus, is αγβ. The presence of the flavin adenine dinucleotide (FAD)-binding motif place Gox as a member of the flavoenzyme family. Furthermore, a negative effect of glucose on the biosynthesis of Gox was observed when it was used as carbon source.Key words: L-glutamate oxidase, gox gene, signal peptide, DNA sequence, flavoenzyme, pIJ702 vector.

Glucose oxidase

10.1139/w01-003

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Citations (6)

Impact assessment of spatial–temporal distribution of riverine dust on air quality using remote sensing data and numerical modeling

Environmental Science and Pollution Research (2024)

Ho‐Wen Chen Chien‐Yuan Chen Guan-Yu Lin

Revegetation

Deposition

AERMOD

Desertification

10.1007/s11356-024-32226-z

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Citations (1)

Improvement in the properties of PAN-based carbon films by modification with cobaltous chloride

Journal of Applied Polymer Science (1999)

Tse‐Hao Ko Chien‐Yuan Chen

Carbon films were developed from polyacrylonitrile (PAN) modified with cobalt chloride. The modification was carried out by immersing PAN in a 5% cobaltous chloride (CoCl2) solution at 90°C for 5 min, oven-dried, and then manufactured into films. The original and modified PAN films were oxidized at 220°C for 2 and 6 h in air, respectively, and finally carbonized at 1300°C. The density, microstructure, elemental analyzer, electrical conductivity, and morphology were all studied. According to the results, it was found that films modified with cobalt chloride have a greater stacking height of carbon-layer planes (Lc), density, electrical conductivity, and nitrogen content after carbonization. Moreover, during the carbonization stage, the cobalt ions promote a catalytic action. The carbon films developed from the modified film not only improved electrical conductivity by 12–38%, but also increased tensile strength by 29–36% and the tensile modulus by 69–110%. Therefore, carbon films having better mechanical properties can be obtained after such modification. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 74: 1745–1751, 1999

Polyacrylonitrile

Carbon fibers

10.1002/(sici)1097-4628(19991114)74:7<1745::aid-app17>3.3.co;2-b

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Citations (1)

Event-based rainfall warning regression model for landslide and debris flow issuing

Environmental Earth Sciences (2020)

Chien‐Yuan Chen

Debris flow

Typhoon

10.1007/s12665-020-8877-9

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Citations (24)

The composition properties of wood vinegar produced from pyrolyzed palm kernel shell waste

CRC Press eBooks (2021)

Chien‐Yuan Chen Hsi-Shou Lee Huann-Ming Chou

In this study, an anoxic pyrolysis mass production process, incorporating the application of the near-slow pyrolysis method, was used to decompose palm kernel shell waste at 500–550℃ to produce biochar, biogas, and liquid wood tar mixture products. The liquid wood tar mixture was stood for phase separation, then distilled to produce heavy metal-free wood vinegar with a pH value of about 3.5~4.1 and organic matter content of about 5.5%, of which the nitrogenous organic matter content was 0.2%. Further GC/MS analyses revealed its main component to be acetic acid and the presence of at least 32 kinds of organic substances, such as phenols, nitrogen-containing compounds, etc. Throughout the storage period, the color changed from light yellow to brown and the organic components also changes via oxidation or reduction reaction.

Palm kernel

Kernel (algebra)

10.1201/9781003188513-24

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Cloning and Characterization of the Replicon of the Nocardia italica Plasmid, pNI100

Plasmid (2000)

Yu‐Tien Liu Chih-Mao Su Chao-Huer Lee Meng‐Jiun Sui Yu-Hsiu Chang

Replicon

T-DNA Binary system

Shuttle vector

Rolling circle replication

Plasmid preparation

Cloning vector

10.1006/plas.1999.1445

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Citations (6)

Combinatorial discovery of thermoresponsive cycloammonium ionic liquids

Chemical Communications (2020)

Yen‐Ho Chu Chun-Chieh Hwang Chien‐Yuan Chen Min‐Jen Tseng

This work demonstrated, for the first time, the combinatorial discovery and rational identification of small-molecule cycloammonium-based thermoresponsive ionic liquids that exhibit LCST phase transition and carry attractive T_c values in water.

10.1039/d0cc04417b

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Citations (3)

ESTIMATION OF SOIL MOBILITY USING THE FALL CONE TEST

International Multidisciplinary Scientific GeoConference SGEM ... (2011)

Chien‐Yuan Chen

10.5593/sgem2015/b12/s2.041

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Raman spectroscopic study of the microstructure of carbon films developed from cobalt chloride-modified polyacrylonitrile

Journal of Applied Polymer Science (1999)

Tse‐Hao Ko Chien‐Yuan Chen

Polyacrylonitrile (PAN) was modified with cobalt chloride at 90°C for 5 min. The carbon films prepared from original and modified PAN films were carbonized up to 1300°C. The structure of the resulting carbon film was studied using X-ray diffraction and Raman spectroscopy. The stacking size obtained from X-ray diffraction approaches the Lc value of the resulting carbon films as the heat treatment temperature increased. The mean average carbon basal planes in crystalline (Lc/d) also increased with increasing pyrolysis temperature. Raman spectra confirmed the progressive structural ordering as treatment temperature increased. During pyrolysis, a substantial decrease in the intensity of the band near the 1350 cm−1 region was observed, indicating a decrease in the disordered structure. The crystal size (La) of the resulting carbon films also showed a remarkable increase with increased heat treatment temperature. The resulting carbon films developed from the modified PAN films had higher Lc and La than those developed from the original PAN film. It was established that cobalt catalyzes graphitization of amorphous carbon during pyrolysis. This modification not only promoted the growth of crystal size but also increased the close packing of the carbon basal planes. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 71: 2219–2225, 1999

Polyacrylonitrile

Carbon fibers

Amorphous carbon

10.1002/(sici)1097-4628(19990328)71:13<2219::aid-app12>3.0.co;2-w

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Citations (20)

Development of a conductivity-based immunosensor for sensitive detection of methamphetamine (stimulant drug) in human urine

Biosensors and Bioelectronics (1996)

Kaoru Yagiuda Akihide Hemmi Satoshi Ito Yasukazu Asano Yoshito Fushinuki

Stimulant

Linear range

10.1016/0956-5663(96)85920-3

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Citations (74)