Electrospun films (ESF) are gaining attention for active delivery due to their biocompatibility and biodegradability. This study investigated the impact of adding soy protein amyloid fibrils (SAFs) to ESF. Functional ESF based on SAFs/pullulan were successfully fabricated, with SAFs clearly observed entangled in the electrospun fibers using fluorescence microscopy. The addition of SAFs improved the mechanical strength of the ESF threefold and increased its surface hydrophobicity from 24.8° to 49.9°. Moreover, the ESF demonstrated antibacterial properties against Escherichia coli and Staphylococcus aureus. In simulated oral disintegration tests, almost 100% of epigallocatechin gallate (EGCG) dissolved within 4 min from the ESF. In summary, the incorporation of SAFs into ESF improved their mechanical strength, hydrophobicity, and enabled them to exhibit antibacterial properties, making them promising candidates for active delivery applications in food systems. Additionally, the ESF showed efficient release of EGCG, indicating their potential for controlled release of bioactive compounds.
Natural soy oleosomes are known to have a remarkable stability, given the advantage of their sophisticated membrane. The aim of the present study is to examine the concept of fabricating a β-carotene emulsion stabilized by soy oleosin (OLE) and lecithin (LEC) mixtures mimicking the membrane composition of soy oleosomes while providing preferable stability and bioaccessibility. For this, the fabricated emulsion was characterized in terms of droplet size distribution, and emulsion structure, stability and digestion (release and absorption of lipophilic β-carotene). Compared to SPI/LEC (10 : 1) stabilized emulsions, the OLE/LEC (10 : 1) mixture stabilized emulsion exhibited the highest emulsifying activity index (EAI) and emulsifying stability index (ESI) values, and higher encapsulation efficiency. Results show that the β-carotene emulsion stabilized by OLE and LEC mixtures at the ratio of 10 : 1 (w/w) has the most uniform droplet distribution and highest stability. The in vitro gastrointestinal digestion test revealed that the β-carotene emulsion stabilized by OLE and LEC mixtures was digested more rapidly than the emulsion stabilized by soy protein isolate (SPI) and LEC mixtures. In turn, the bioaccessibility and cellular uptake of β-carotene were enhanced, resulting in a higher absorption, a desirable feature of nutrition delivery systems. Our results demonstrated a promising way to fabricate emulsions mimicking natural soy oleosomes.
Peroxpolytungstic acid (PPTA) is one of the important precursors to synthesize nanostructured tungsten oxides with the chemical routes. In this paper，PPTA sol, being prepared with H2O2, W and C2H5OH, was placed at room temperature for a long time till it was jellified naturally and then was dried at 120 ℃ for 3 hours for this investigation. Structures, thermal stability and optical UV-Vis absorption of the samples have been investigated by XRD, SEM, Raman, TG/DSC and UV-Vis spectrum, respectively. The wide peaks from XRD indicate that the sample is amorphous. The Gaussian fitting for these XRD peaks indicates that the sample is the composite of tungsten oxides and tungsten oxide hydrates. SEM indicates that the sample possesses the morphologies of the nano-particles with the sizes of about 50~100 nm and the nano-flakes with the thickness of about 50 nm. The Gaussian fitting of the wide Raman peaks illustrates that the sample possesses the obvious the modes of symmetrical O—W—O stretching, asymmetrical O—W—O stretching and WO vibration accompanied with the modes of symmetrical O—W—O bending, asymmetrical O—W—O bending and the adsorptive water vibration. This fact further indicates that the sample is composed of the amorphous tungsten oxides and tungsten oxide hydrates. Analysis on the TG/DSC curves indicates that PPTA gels possess four different thermal dynamic processes with increasing the treatment temperature from 120 to 500 ℃: (1) the slow crystallization of PPTA (120~165 ℃), (2) the dissociation of adsorptive H2O2 and the desorption of adsorptive H2O (165~236 ℃), (3) the quick decomposition of tungsten oxide hydrate (236~287 ℃) and (4) the crystallization and phase transformation of the final products WO3 (287~500 ℃). Optical absorption of PPTA gels happens in the range of 350~600 nm, where the intensity of the optical absorption gradually increases and finally reaches the saturation with the increase of photon energy. The optical band gap was estimated to be about 2.25 eV, being obviously lower than the known values for WO3 and H2WO4 (2.48~3.50 eV). The key factors for the low gap value of the composite can be attributed to the molecular water, the oxygen defects and the structural distortion.
Abstract To address the unique challenges of diabetic wound healing, wound dressings, particularly multifunctional hydrogels have garnered considerable interest. For the first time, a novel environmentally friendly soy protein‐based hydrogel is developed to accelerate the healing of diabetic chronic wounds. Specifically, this hydrogel framework is in direct formation through the dynamic Schiff base between oxidized guar gum and epigallocatechin‐3‐gallate (EGCG)‐modified soy protein isolate. Meantime, the addition of Ag + enhances the cross‐linking of the hydrogel network by forming metal‐ligand bonds with the catechol groups in EGCG. Interestingly, the stretchability (up to 380%), swelling, and rheology properties of the hydrogel can be controlled by fine‐tuning the density of metal‐ligand bonds, endowing them with a high potential for precise matching. Additionally, various dynamic bonds endow hydrogel with excellent self‐healing ability, adhesiveness, and injectability. This hydrogel also exhibits good antibacterial properties, biocompatibility, and cell migration capabilities. Both in vivo and in vitro experiments demonstrated the outstanding anti‐inflammatory capacity of the hydrogel and its ability to modulate macrophage polarization. Consequently, the hydrogel has proven effective in promoting wound healing in a diabetic full‐thickness wound model through enhanced angiogenesis and collagen deposition. This eco‐friendly plant protein hydrogel offers a sustainable solution for wound care and environmental protection.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)