In this report 40 newly observed GC/DBP mutants are described. A list of the thus far identified GC mutants is presented: in addition to the three common alleles, a total of 124 Gc variants are recorded. Their population distribution is described and their relationship to the molecular features of the DBP protein is discussed. The methods currently in use for the delineation of GC mutants are briefly considered.
Abstract The genetic variability of the human group‐specific component (Gc) system has been examined by isoelectric focusing with immobilized pH gradients. The phenotypes of the six common Gc types and of 15 rare genetic Gc variants are described and presented. High resolution of the Gc components is accomplished. The classification can be made without specific immune reaction. Isoelectric focusing with immobilized pH gradients represents a powerful tool for analyzing genetic variability.
Abstract Human peripheral blood lymphocytes were isolated from healthy donors and cultivated in the absence and presence of phytohemagglutinin (PHA) or pokeweed mitogen (PWM). Cell lysates of unlabelled lymphocytes or following stimulation of [H]leucine metabolically labelled lymphocytes were subjected to two‐dimensional electrophoresis. Differences in the protein patterns are described which are related to the method applied for visualization of the proteins (silver staining or fluorography), to the culture conditions (unstimulated, PHA or PWM stimulated lymphocytes) and to individual variations. Comparative analysis of the resulting patterns on silver stained gels and fluorograms revealed three qualitative and several quantitative differences. Comparing the two‐dimensional patterns of unstimulated and mitogenstimulated lymphocytes with silver staining, we recognized the quantitative increase of 6 protein spots after stimulation. Whereas protein patterns of PHA and PWM stimulated cells showed only minor quantitative differences when compared in silver stained gels, one qualitative and two quantitative differences between the two mitogens could be observed in fluorograms. Individual variability was generally low: within the approximately 30 unrelated individuals examined, thus far, the presence of two polymorphic proteins was demonstrated.
See also: Zur Frage der Bedeutung des H-Y-Antigens für die Gonadendetermination und für die Differenzierung der GeschlechtsidentitätGeburtshilfe Frauenheilkd 1981; 41(01): 58-60DOI: 10.1055/s-2008-1036843
The human p60 (Mr 60,000) is an abundant protein in the two-dimensional electrophoresis pattern of the cellular proteins of human mitogen-stimulated lymphocytes. The p60 shows as remarkable characteristic a genetic polymorphism with two different alleles. Electrotransfer of this protein from two-dimensional gels onto siliconized glass fiber sheets and subsequent amino-acid sequence analysis has revealed a striking homology to the known bacteria and plant chaperonins, the groEL and the Rubisco-subunit-binding protein. From this sequence homology we conclude that we have identified the human chaperonin homologue.
