Nadège Matthey

Swiss Center for Electronics and Microtechnology (Switzerland)

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Martha Liley

Swiss Center for Electronics and Microtechnology (Switzerland)

Gilles Weder

Swiss Center for Electronics and Microtechnology (Switzerland)

H. Heinzelmann

Swiss Center for Electronics and Microtechnology (Switzerland)

M. Giazzon

Swiss Center for Electronics and Microtechnology (Switzerland)

János Vörös

ETH Zurich

Raphaël Pugin

Swiss Center for Electronics and Microtechnology (Switzerland)

Orane Guillaume‐Gentil

ETH Zurich

Nicolas Blondiaux

Swiss Center for Electronics and Microtechnology (Switzerland)

Mona Julia Katharina Klein

IBM Research - Zurich

Franck Montagne

Roche (Switzerland)

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Swiss Center for Electronics and Microtechnology (Switzerland)

École Polytechnique Fédérale de Lausanne

ETH Zurich

Institute for Biomedical Engineering

University of Lausanne

Roche (Switzerland)

Instituttet for Produktudvikling (Denmark)

University of Arizona

Centre National de la Recherche Scientifique

University of Basel

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Measuring cell adhesion forces during the cell cycle by force spectroscopy

Biointerphases (2009)

Gilles Weder János Vörös M. Giazzon Nadège Matthey H. Heinzelmann

Force spectroscopy has been used to measure the adhesion of Saos-2 cells to a glass surface at different phases of the cell cycle. The cells were synchronized in three phases of the cell cycle: G1, S, and G2M. Cells in these phases were compared with unsynchronized and native mitotic cells. Individual cells were attached to an atomic force microscope cantilever, brought into brief contact with the glass surface, and then pulled off again. The force-distance curves obtained allowed the work and maximum force of detachment as well as the number, amplitude, and position of discrete unbinding steps to be determined. A statistical analysis of the data showed that the number of binding proteins or protein complexes present at the cell surface and their binding properties remain similar throughout the cell cycle. This, despite the huge changes in cell morphology and adhesion that occur as the cells enter mitosis. These changes are rather associated with the changes in cytoskeletal organization, which can be quantified by force spectroscopy as changes in cell stiffness.

Force Spectroscopy

10.1116/1.3139962

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Use of Force Spectroscopy to Investigate the Adhesion of Living Adherent Cells

Langmuir (2010)

Gilles Weder Nicolas Blondiaux M. Giazzon Nadège Matthey Mona Julia Katharina Klein

The use of force spectroscopy to study the adhesion of living fibroblasts to their culture substrate was investigated. Both primary fibroblasts (PEMF) and a continuous cell line (3T3) were studied on quartz surfaces. Using a fibronectin-coated AFM cantilever, it was possible to detach a large proportion of the 3T3 cells from the quartz surfaces. Their adhesion to the quartz surface and the effects of topography on this adhesion could be quantified. Three parameters characteristic of the adhesion were measured: the maximum force of detachment, the work of adhesion, and the distance of detachment. Few PEMF cells were detached under the same experimental conditions. The potential and limitations of this method in measuring cell/surface interactions for adherent cells are discussed.

Force Spectroscopy

10.1021/la904526u

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Citations (33)

The quantification of single cell adhesion on functionalized surfaces for cell sheet engineering

Biomaterials (2010)

Gilles Weder Orane Guillaume‐Gentil Nadège Matthey Franck Montagne H. Heinzelmann

Force Spectroscopy

Morphology

10.1016/j.biomaterials.2010.04.068

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Citations (39)