Anna Kümmecke

Rawdata used for pSAS Implementation, simulation SAS parameter study and FORID function presentation. Included datasets: rawdata_Apgar.xlsx: Raw data for pSAS Implementation sas_example.xlsx: Excerpt from rawdata_Apgar.xlsx to be used in simulation parameter study upload_example.xlsx: Example used to demonstrate data upload procedure to FORID database mobps_example.xlsx: Example used to demonstrate data forwarding from FORID database to MoBPS analysis tool

10.5281/zenodo.5070666

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Datasets used in European Surgical Research special edition on severity assessment (2021)

Zenodo (CERN European Organization for Nuclear Research) (2021)

Stefan Bruch Lisa Ernst Anna Kümmecke Leonie Zieglowski Wenjia Liu

Rawdata used for pSAS Implementation, simulation SAS parameter study and FORID function presentation. Included datasets: rawdata_Apgar.xlsx: Raw data for pSAS Implementation sas_example.xlsx: Excerpt from rawdata_Apgar.xlsx to be used in simulation parameter study upload_example.xlsx: Example used to demonstrate data upload procedure to FORID database mobps_example.xlsx: Example used to demonstrate data forwarding from FORID database to MoBPS analysis tool

10.5281/zenodo.5070667

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Assessing the severity of laparotomy and partial hepatectomy in male rats – a multimodal approach

Zenodo (CERN European Organization for Nuclear Research) (2020)

Leonie Zieglowski Anna Kümmecke Lisa Ernst Rupert Palme Ralf Weiskirchen

A total of 136 adult male WistarHan rats were randomly assigned to three groups: Sham-laparotomy (Sham) group [7 days post-surgical survival time (n = 10)]; 50% partial hepatectomy (PH) group; 70% PH group. Both PH groups had three postoperative survival times of 1, 3, or 7 days (n = 21 for each time-point). Postoperative severity assessment was performed daily using a model-specific score sheet, behavioral testing in the Open Field (OF), telemetric data analysis, and analysis of blood and fecal samples. Parameters of surgical and postoperative distress and severity in the animals were analyzed. Rats were trained to the OF test thrice every alternate training day (D-20, D-18, and D-16). On D-15 (surgery I), a telemetric transmitter (hereafter referred to as transmitter implantation, TI) was surgically implanted subcutaneously in the left flank in 12 animals of each 7-day survival group. All animals in the Sham group were implanted a dummy device. Transmitter implantation was followed by a 12-day recovery phase with no further intervention. To ensure comparability, this test-free recovery period was standardized for all animals, even if they were not telemetrically monitored. Thereafter, retraining was performed on D-2 and -1 in all animals, followed by Sham, 50% PH, or 70% PH (surgery II), on D0. OF tests were performed on POD1, POD3, POD4, and POD7 (depending on survival time). Body weight was measured before individual training, as well as before and after each surgery; in addition, the body weight was measured once daily during postoperative scoring. Postoperative scoring was conducted three times a day from POD1 to POD3 and once a day in the morning from POD4 to POD7. On euthanasia day (POD1, POD3, or POD7) rats were reopened (surgery III) under general anesthesia and analgesia and were euthanized by final blood withdrawal from the inferior vena cava. Organs were removed for subsequent examinations and processed accordingly.

10.5281/zenodo.3842977

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Does Sex Matter in Liver Surgery? Comparison of Severity Assessments between Female and Male Rats after Partial Hepatectomy - A Pilot Study

Zenodo (CERN European Organization for Nuclear Research) (2021)

Anna Kümmecke Leonie Zieglowski Lisa Ernst Rupert Palme René H. Tolba

Raw data.

10.5281/zenodo.6475184

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Severity assessment using three common behavioral or locomotor tests after laparotomy in rats: a pilot study

Laboratory Animals (2020)

Leonie Zieglowski Anna Kümmecke Lisa Ernst Mareike Schulz Steven R. Talbot

The aim of this pilot study was to evaluate whether behavioral or locomotor tests (Open Field (OF), rotarod (RR), and CatWalk (CW)) can help assess the severity of laparotomy in rats. The new EU Directive (2010/63/EU) mandates severity assessment in experiments involving animals. However, validated and objective methods are needed to relate trial-specific monitoring results to the degree of distress caused to individual animals. Therefore, we focused on non-invasive or minimally invasive, simple, and convenient severity assessment methods in a surgical model. To evaluate surgical severity in this model, we compared moving velocity among three commonly used behavioral test methods (OF, RR, and CW) after midline laparotomy within postoperative 7 days. In this study, 30 adult male Wistar Han rats ( n = 10 per test) were trained in their assigned test method and subsequently subjected to surgery. Severity scoring was performed daily using a modified score sheet developed previously. In addition, blood and fecal samples were collected to analyze surgical and postoperative corticosterone metabolite levels. We found significant differences among the experimental groups in terms of the analyzed parameters. In this context, the OF test was found to be the most suitable method for severity assessment after laparotomy in rats.

10.1177/0023677220911680

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Re-Sterilisation of Single-Use Telemetric Devices

European Surgical Research (2021)

Leonie Zieglowski Anna Kümmecke René H. Tolba Lisa Ernst

Implantable telemetric transponders for contactless measurement of physiological parameters are often used in animal-based research. After explantation, single-use devices cannot be re-implanted because of non-validated functionality and necessary re-sterilisation. This is disadvantageous because the battery life would enable a second implantation cycle in another animal. To save costs and time taken for the manufacturer's refurbishing process, we validated and implemented a re-sterilisation protocol for single-use transponders using hydrogen peroxide gas. The described protocol was established with models, i.e., for large (n = 7) and small (n = 3) animals, of telemetric device from 2 different manufacturers (Data Science International and EMKA). All transponders, prepared according to the protocol, were previously implanted subcutaneously in the flank of pigs or rats for a duration of 21 days. Our investigations demonstrate that disinfection only is not sufficient against bacterial contamination and that sterility can only be achieved by additional gas sterilisation with hydrogen peroxide. Furthermore, re-implantation of the re-sterilised transponders into pigs caused neither undesired tissue reactions along the transponder nor impairment of the measured values when compared to the first implantation and after necropsy in 4 cases. We were able to demonstrate that, using our protocol, re-implantation of reprocessed single-use telemetric devices can be performed without compromising transponder quality.

Transponder (aeronautics)

Single use

10.1159/000516829

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Does Sex Matter in Liver Surgery? Comparison of Severity Assessments between Female and Male Rats after Partial Hepatectomy - A Pilot Study

Zenodo (CERN European Organization for Nuclear Research) (2021)

Anna Kümmecke Leonie Zieglowski Lisa Ernst Rupert Palme René H. Tolba

Raw data.

10.5281/zenodo.5118655

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Assessing the severity of laparotomy and partial hepatectomy in male rats – a multimodal approach

Zenodo (CERN European Organization for Nuclear Research) (2020)

Leonie Zieglowski Anna Kümmecke Lisa Ernst Rupert Palme Ralf Weiskirchen

A total of 136 adult male WistarHan rats were randomly assigned to three groups: Sham-laparotomy (Sham) group [7 days post-surgical survival time (n = 10)]; 50% partial hepatectomy (PH) group; 70% PH group. Both PH groups had three postoperative survival times of 1, 3, or 7 days (n = 21 for each time-point). Postoperative severity assessment was performed daily using a model-specific score sheet, behavioral testing in the Open Field (OF), telemetric data analysis, and analysis of blood and fecal samples. Parameters of surgical and postoperative distress and severity in the animals were analyzed. Rats were trained to the OF test thrice every alternate training day (D-20, D-18, and D-16). On D-15 (surgery I), a telemetric transmitter (hereafter referred to as transmitter implantation, TI) was surgically implanted subcutaneously in the left flank in 12 animals of each 7-day survival group. All animals in the Sham group were implanted a dummy device. Transmitter implantation was followed by a 12-day recovery phase with no further intervention. To ensure comparability, this test-free recovery period was standardized for all animals, even if they were not telemetrically monitored. Thereafter, retraining was performed on D-2 and -1 in all animals, followed by Sham, 50% PH, or 70% PH (surgery II), on D0. OF tests were performed on POD1, POD3, POD4, and POD7 (depending on survival time). Body weight was measured before individual training, as well as before and after each surgery; in addition, the body weight was measured once daily during postoperative scoring. Postoperative scoring was conducted three times a day from POD1 to POD3 and once a day in the morning from POD4 to POD7. On euthanasia day (POD1, POD3, or POD7) rats were reopened (surgery III) under general anesthesia and analgesia and were euthanized by final blood withdrawal from the inferior vena cava. Organs were removed for subsequent examinations and processed accordingly.

10.5281/zenodo.3842976

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Does Sex Matter in Liver Surgery? Comparison of Severity Assessments between Female and Male Rats after Partial Hepatectomy - A Pilot Study

Zenodo (CERN European Organization for Nuclear Research) (2021)

Anna Kümmecke Leonie Zieglowski Lisa Ernst Rupert Palme René H. Tolba

Raw data.

Adult male

10.5281/zenodo.5118654

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Does Sex Matter in Liver Surgery? Comparison of Severity Assessments between Female and Male Rats after Partial Hepatectomy: A Pilot Study

European Surgical Research (2022)

Anna Kümmecke Leonie Zieglowski Lisa Ernst Rupert Palme René H. Tolba

Current animal-based biomedical research, including studies on liver function and disease, is conducted almost exclusively on male animals to mitigate confounding effects of the estrous cycle. However, liver diseases afflict both men and women, so translational research findings should also be applicable to female patients. This pilot study investigated sex differences in objective and subjective severity assessment parameters in rats following 50% partial hepatectomy.This study was performed using Wistar Han rats, in which measurements of body weight, spontaneous motor activity in the open field (OF) (movement distance, movement velocity, rearing frequency), and fecal corticosterone metabolites were conducted at baseline and at multiple times after partial hepatectomy. Subjective postsurgical severity assessments were conducted using modified score sheets. Blood parameters such as leukocyte count and serum aspartate aminotransferase, as well as estrogens and testosterone were measured from samples obtained during partial hepatectomy and at sacrifice. In addition, the amount of resected liver tissue was measured at partial hepatectomy, and the proliferated liver was weighed at sacrifice.Fecal corticosterone metabolite concentrations differed significantly between males and females at baseline and following hepatectomy. Also, leukocyte counts and estrogen concentrations were significantly different between sexes before partial hepatectomy. Alternatively, there were no sex differences in severity assessments, body weight changes, and behavior in the OF at any measurement time point. Liver weight was significantly different in males and females at the time point of partial hepatectomy and sacrifice.The results of this pilot study suggest that males and females respond similarly following partial hepatectomy. Examination of both sexes is very important for translation to humans, where both men and women suffer from liver disease. Furthermore, the use of both sexes in animal-based research would improve the utilization of the animal breeding in terms of the 3 Rs. However, due to some limitations, larger scale investigations including a broader spectrum of pathophysiolological, behavioral, and pharmacokinetic measures are planned.

Corticosterone

10.1159/000527334

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Anna Kümmecke

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