Abstract Background: Long non-coding RNA (lncRNA) plays an important role in carcinoma progression and prognosis. However, little is known about the pathological role of lncRNA Sox2ot in colorectal cancer (CRC) patients. This study sought to investigate the expression level of lncRNA Sox2ot in CRC, as well as explore its association with CRC progression and prognosis. Methods: LncRNA Sox2ot expression was measured in 117 paired CRC tissues and adjacent non-cancerous tissues using quantitative real-time polymerase chain reaction (qRT-PCR). Then, the relationship between lncRNA Sox2ot expression and clinicopathological characteristic of CRC patients was detected by Chi-square test. Lastly, relationship between the expression level of lncRNA Sox2ot and overall survival (OS) was analyzed using Kaplan–Meier analysis and univariate and multivariate analyses were evaluated by Cox regression models. Results: The expression level of lncRNA Sox2ot in CRC tissues were significantly higher than in the adjacent non-cancerous tissues (P<0.05). In addition, high lncRNA Sox2ot expression was positively correlated with N stage, T stage, TMN stage, distant metastasis, histological differentiation grade and lymph node involvement in CRC patients (all, P<0.05). Kaplan-Meier analysis showed that patients with high expression of lncRNA Sox2ot had a shorter OS than those with low lncRNA Sox2ot expression (log rank test, P<0.001). Moreover, in univariate and multivariate Cox regression models, lncRNA Sox2ot expression was an independent prognostic factor for CRC patients. Conclusion: LncRNA Sox2ot over-expression may serve as an unfavorable prognosis predictor for CRC patients.
Abstract Background The HOXA9 gene, belonging to homeobox ( HOX ) gene family, has been recently reported dys-expressed in several kinds of human cancers. This study aimed to investigate the expression of HOXA9 and its prognostic value in cervical cancer. Methods The HOXA9 mRNA expression was detected with a quantitative real-time polymerase chain reaction (qRT-PCR) assay, and the association of HOXA9 expression with clinical characteristic was analyzed via chi-square test. Kaplan-Meier and cox regression analyses were conducted to estimate the prognostic value of HOXA9 in cervical cancer. Results HOXA9 expression was significantly down-expressed in cervical cancer tissues compared with that in adjacent normal tissues ( P < 0.01). And the expression of HOXA9 was significantly associated with TNM stage, pathological grade, FIGO stage and differentiation (All P < 0.05). In addition, Kaplan–Meier analysis indicated that the overall survival of patients with low HOXA9 expression was shorter than those with high HOXA9 expression (log rank test, P = 0.000). Cox regression analysis revealed that HOXA9 had a high prognostic value in cervical cancer. Conclusion HOXA9 is down-regulated and involved in the development of cervical cancer. Moreover, it may be an useful independent prognostic bio-marker for patients with cervical cancer.
Angiotensin I-Converting Enzyme (ACE, CD143) Gene plays a crucial role in the pathology of carcinomas in many cancers including colorectal cancer (CRC). However, the methylation of ACE was rarely reported. In this study, our purpose was to investigate the methylation status of ACE and explored its prognostic value in CRC. The expression of ACE was detected by quantitative real-time polymerase chain reaction (qRT-PCR) analysis while the methylation status of ACE was measured via methylation-specific polymerase chain reaction (MSP). The result demonstrated that ACE expression was up-regulated in tumour tissues and HT-29 cells compared with the controls. ACE was also confirmed to be hypomethylated in CRC. Next, we evaluated the influence of ACE hypomethylation on cell growth. It was proved to be a favourable factor for the cell proliferation, cell colony forming, but an inhibitor for the cell apoptosis of CRC cells according to MST assay, colony forming assay and flow cytometry assay. ACE hypomethylation was also considered to be related to the prognosis of CRC through Cox regression analysis. Taken together, the over-expression of ACE was regulated by its hypomethylation and the ACE hypomethylation might be an independent prognostic indicator in CRC.
Abstract Background: Breast cancer is one of the most common cancers among females with high morbidity and mortality. MicroRNAs (miRNAs) have been reported to play important roles in the development of cancers. However, the prognostic value of microRNA-421 ( miR-421 ) in breast cancer have not been extensively explored. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the relative expression of miR-421 in breast cancer tissue samples. Relationships between miR-421 expression and clinicopathological factors were analyzed by chi-square test. The effects of several variables on survival status were tested by Kaplan-Meier curve and Cox proportional hazards regression analyses. Results: MiR-421 expression was significantly decreased in breast cancer tissues, compared with adjacent noncancerous tissues ( P <0.001). Moreover, abnormal miR-421 expression was closely correlated with lymph node metastasis ( P <0.001), TNM stage ( P =0.021), and differentiation ( P =0.044) of breast cancer patients. Kaplan-Meier analysis revealed that patients with low miR-421 expression had a shorter overall survival time than those with high miR-421 expression ( P =0.001). Furthermore, multivariate analysis demonstrated that miR-421 ( P =0.014, HR=2.000, 95%CI: 1.149-3.480) was an independent prognostic indicator in breast cancer patients, as well as lymph node metastasis ( P =0.016, HR=1.987, 95%CI: 1.137-3.474). Conclusion: The reduced expression of miR-421 may predict the poor prognosis of breast cancer and miR-421 may be involved in the progression of breast cancer.
Abstract Background: The study was performed to compare the diagnostic roles of computed tomography (CT) and carcinoembryonic antigen (CEA) in colorectal liver metastasis (CRLM). Methods: 255 patients with colorectal cancer (CRC) were enrolled. These patients were confirmed as CRLM by histopathological assay. CT scans of the liver were performed with a 64-slice CT system. Serum CEA levels were determined using a human circulating cancer biomarker magnetic bead panel. True positive (TP), false positive (FP), true negative (TN) and false negative (FN) were calculated for CT and CEA with histopathological assay as golden standard. Results: 142 CRLM patients and 113 non-CRLM patients were confirmed in the study. There were no obvious differences in age, sex and Dukes stage between CRLM and non-CRLM patients ( P ＞0.05). Diagnostic roles of CT and CEA on per-patient and per-lesion were analyzed. Detection sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of CT on per-patient basis were 74.7%, 56.4%, 52.1%, and 77.9%, respectively. Sensitivity, specificity, PPV and NPV of CEA were 64.4%, 51.4%, 49.3%, and 67.3%. In the analysis on per-lesion basis, detection sensitivity, specificity, PPV, and NPV of CT were increased to 87.8%, 88.8%, 91.5%, and 84.1%, respectively. Detection sensitivity, specificity, PPV and NPV of CEA on per-lesion basis were 82.6%, 64.4%, 63.4%, and 83.2%. Conclusion: CT exhibited better performances than CEA in diagnosis of CRLM on both of per-patient and per-lesion basis.
Abstract Background : This study was conducted to detect the expression of Cdc42 interacting protein 4 ( CIP4 ) in patients with colorectal cancer (CRC), and explore the role of CIP4 in prognosis of CRC patients. Methods : The expression of CIP4 mRNA was determined by quantitative real-time PCR (qRT-CPR) and compared by student’s t-test between groups. Relationships of clinical characteristics and CIP4 expression were analyzed by Chi-square test. Kaplan-Meier curves were used to estimate the overall survival of CRC patients. And Cox regression analysis was conducted to identify the prognostic biomarkers for CRC patients. Results : The qRT-PCR results showed that CRC tissues were detected with significantly high CIP4 mRNA expression compared with adjacent normal controls ( P <0.0001). The overexpression of CIP4 in CRC tissues was influenced by distant metastasis ( P =0.021), lymphatic invasion ( P =0.012) and TNM stage ( P =0.006). But, other clinical factors including age, gender, differentiation and tumor site were proved to have no obvious effects on CIP4 expression (all, P >0.05). The survival curves showed that patients with high CIP4 expression generally lived shorter than those with low CIP4 expression ( P <0.001). In addition, the multivariate analysis revealed that differentiation ( P =0.044, HR=1.631, 95%CI=1.013-2.626) and CIP4 expression ( P =0.000, HR=5.283, 95%CI=3.138-8.893) were of great prognostic significance for CRC patients. Conclusion : Taken together, up-regulation of CIP4 in CRC tissues represented poor prognosis for patients.
Abstract Backgrounds: Serum microRNAs (miRNAs) are a novel class of diagnostic biomarkers for human cancers. However, the clinical relevance of circulating microRNA-429 ( miR-429 ) in cervical cancer patients remains unclear. The purpose of this study was to investigate serum miR-429 levels in cervical cancer patients, as well as its association with clinical characteristics and diagnostic value. Methods: Serum miR-429 expression was investigated in 113 cervical cancer patients and 107 healthy normal controls by quantitative real-time PCR (qRT-PCR). Chi-square test was applied to evaluate the correlation between miR-429 expression and clinicopathological parameters. Receiver operating characteristic (ROC) curves were constructed to assess the diagnostic significance of serum miR-429 in cervical cancer. Results: Serum miR-429 expression was significantly down-regulated in cervical cancer compared with healthy controls ( P =0.000). In addition, serum miR-429 level was correlated with differentiation ( P =0.031), FIGO stage ( P =0.030), and lymph node metastasis ( P =0.045). ROC curves demonstrated that serum miR-429 expression could distinguish cervical cancer patients from healthy controls with the sensitivity of 81.4% and the specificity of 69.2%. Additionally, the area under the curve (AUC) was 0.816 and the cut-off value was 0.825. Conclusion: MiR-429 is down-regulated in cervical cancer and associated with aggressive clinical characteristics. MiR-429 may be a potential diagnostic biomarker for cervical cancer.
Abstract Background : Our study was designed to explore the diagnostic role of serum microRNA-218 (miR-218) in colorectal cancer (CRC). Methods : Serum level of miR-218 was measured in 117 CRC samples and 88 normal controls using quantitative reverse transcription (qRT-PCR). Chi-square test was performed to assess the relationships between miR-218 expression and clinical characteristics of CRC patients. The receiver operating characteristic (ROC) analysis was established to investigate the diagnostic significance of miR-218 in CRC with the area under ROC curve (AUC). Results : MiR-218 was found to be weekly expressed in CRC serum samples compared with healthy controls ( P < 0.001). And the down-regulation of miR-218 shared close relationships with lymph node metastasis ( P = 0.013), vascular invasion ( P = 0.020) and TNM stage ( P = 0.031). However, age, tumor size and gender had no significant influence on miR-218 expression ( P > 0.05). According to the ROC curve, miR-218 yielded an AUC value of 0.897 and an optimal cutoff point value of 0.021, providing a 83.8% sensitivity and a 78.4% specificity. Conclusion : In summary, decreased expression of serum miR-218 was detected in CRC patients and the expression of miR-218 was a diagnostic marker in CRC.
Abstract Background: Gastric cancer (GC) represents one of the most serious cancers worldwide with the increasing mortality. Metastasis associated lung adenocarcinoma transcript 1 (MALAT1), a kind of lncRNAs, has been reported to be involved in the progression of cancers. This study aimed to assess serum expression pattern of MALAT1 and its clinical significance in diagnosis of GC. Methods: Serum specimens were collected from 120 GC patients and 58 healthy individuals. The expression profile of MALAT1 was examined using quantitative real-time polymerase chain reaction (qRT-PCR), and its association with clinical parameters was estimated by chi-square test. The diagnostic value of MALAT1 in GC was evaluated by the receiver operating characteristic (ROC) analysis. Results: Upregulated expression of MALTA1 was found in GC patients compared with the healthy controls (P<0.05). The overexpression of MALAT1 was positively correlated with lymph node metastasis (P=0.041) and TNM stage (P=0.005). An area under the curve (AUC) was 0.897 in ROC analysis, suggesting the high diagnostic value of MALAT1. Conclusion: The expression of MALAT1 was upregulated in GC serum samples, and its expression might serve as a potential diagnostic biomarker in patients with GC.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)