Yan Zhou

Qingdao University of Science and Technology

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Wen‐Song Tan

East China University of Science and Technology

Zhaoyang Ye

Hebei North University

Meidong Lang

East China University of Science and Technology

Ce Gu

East China University of Science and Technology

Liang Zhao

East China University of Science and Technology

Miaomiao Chai

East China University of Science and Technology

Yan Xiao

East China University of Science and Technology

Yan Zhang

Xi'an Institute of Optics and Precision Mechanics

Mingqi Tan

China Medical University

Qiaohui Lu

East China University of Science and Technology

Cooperative Institutions

East China University of Science and Technology

148

Chinese Academy of Sciences

101

Sichuan University

Fudan University

Shanghai Jiao Tong University

University of Chinese Academy of Sciences

Soochow University

Huazhong University of Science and Technology

Ministry of Agriculture and Rural Affairs

Jilin University

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Acid-sensing ion channel 3 is required for agmatine-induced histamine-independent itch in mice

Frontiers in Molecular Neuroscience (2023)

Guokun Zhou Wenjing Xu Yi Lu Yan Zhou Chen-Zhang Feng

Itch is a common symptom of many skin and systemic diseases. Identifying novel endogenous itch mediators and the downstream signaling pathways involved will contribute to the development of new strategies for the treatment of chronic itch. In the present study, we adopted behavioral testing, patch clamp recording and metabonomics analysis to investigate the role of agmatine in itch and the underlying mechanism.Behavioral analysis was used to evaluate the establishing of acute and chronic itch mice model, and to test the effects of different drugs or agents on mice itch behavior. Western blotting analysis was used to test the effect of agmatine on phosphorylation of ERK (p-ERK) expression in the spinal cord. Patch clamp recording was used to determine the effect agmatine on the excitability of DRG neurons and the role of ASIC3. Finally, the metabonomics analysis was performed to detect the concentration of agmatine in the affected skin under atopic dermatitis or psoriasis conditions.We fused a mouse model and found that an intradermal injection of agmatine (an endogenous polyamine) into the nape of the neck or cheek induced histamine-independent scratching behavior in a dose-dependent manner. In addition, the ablation of nociceptive C-fibers by resiniferatoxin (RTX) abolished agmatine-induced scratching behavior. However, agmatine-induced itch was not affected by the pharmacological inhibition of either transient receptor potential vanilloid 1 (TRPV1) or transient receptor potential ankyrin 1 (TRPA1); similar results were obtained from TRPV1-/- or TRPA1-/- mice. Furthermore, agmatine-induced itch was significantly suppressed by the administration of acid-sensing ion channel 3 (ASIC3) inhibitors, APETx2 or amiloride. Agmatine also induced the upregulation of p-ERK in the spinal cord; this effect was inhibited by amiloride. Current clamp recording showed that the acute perfusion of agmatine reduced the rheobase and increased the number of evoked action potentials in acute dissociated dorsal root ganglion (DRG) neurons while amiloride reversed agmatine-induced neuronal hyperexcitability. Finally, we identified significantly higher levels of agmatine in the affected skin of a mouse model of atopic dermatitis (AD) when compared to controls, and the scratching behavior of AD mice was significantly attenuated by blocking ASIC3.Collectively, these results provide evidence that agmatine is a novel mediator of itch and induces itch via the activation of ASIC3. Targeting neuronal ASIC3 signaling may represent a novel strategy for the treatment of itch.

Agmatine

Scratching

Resiniferatoxin

10.3389/fnmol.2023.1086285

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Citations (3)

[The effect of antioxidants on the in vitro life-span of keratinocyte].

PubMed (2002)

Yan Zhou Anli Ouyang Ping Hua Wen‐Song Tan

The effect of antioxidants on the in vitro life span of mouse keratinocytes was investigated in this work. It was found that the life span of the keratinocytes cultured in the medium supplemented with antioxidants was extended significantly. The most beneficial antioxidant used in this work was the mercaptoethanol, followed by the catalase and SOD. However, the growth rates of keratinocytes in vitro under all the experimental conditions still declined with the culture time. It was also found that the antioxidants added in the medium were also helpful to enhance the keratinocyte colony formation. In addition, the aging kinetics of the mouse epidermal keratinocytes in vitro were analyzed, and finally the aging rate constants corresponding to antioxidants used were calculated.

Life span

Source

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Fabrication of viable and functional pre‐vascularized modular bone tissues by coculturing MSCs and HUVECs on microcarriers in spinner flasks

Biotechnology Journal (2017)

Songjie Zhang Min Zhou Zhaoyang Ye Yan Zhou Wen‐Song Tan

Abstract Slow vascularization often impedes the viability and function of engineered bone replacements. Prevascularization is a promising way to solve this problem. In this study, a new process was developed by integrating microcarrier culture and coculture to fabricate pre‐vascularized bone microtissues with mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs). Initially, coculture medium and cell ratio between MSCs and HUVECs were optimized in tissue culture plates concerning cell proliferation, osteogenesis and angiogenesis. Subsequently, cells were seeded onto CultiSpher S microcarriers in spinner flasks and subjected to a two‐stage (proliferative‐osteogenic) culture process for four weeks. Both cells proliferated and functioned well in chosen medium and a 1 : 1 ratio between MSCs and HUVECs was chosen for better angiogenesis. After four weeks of culture in spinner flasks, the microtissues were formed with high cellularity, evenly distributed cells and tube formation ability. While coculture with HUVECs exerted an inhibitory effect on osteogenic differentiation of MSCs, with downregulated alkaline phosphatase activity, mineralization and gene expression of COLI , RUNX2 and OCN , this could be attenuated by employing a delayed seeding strategy of HUVECs against MSCs during the microtissue fabrication process. Conclusion: Collectively, this work established an effective method to fabricate pre‐vascularized bone microtissues, which would lay a solid foundation for subsequent development of vascularized tissue grafts for bone regeneration.

Microcarrier

Laboratory flask

10.1002/biot.201700008

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Citations (29)

Effects of Lettuce Glycoside B in Ameliorating Pulmonary Fibrosis Induced by Irradiation Exposure and its Anti-Oxidative Stress Mechanism

Cell Biochemistry and Biophysics (2014)

Yan Zhou Yuan Gao Yingying Chen Rui Zheng Wei Zhang

Hydroxyproline

Malondialdehyde

Pulmonary toxicity

10.1007/s12013-014-0295-8

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Citations (7)

Deep insight into the key role of carbon black self-networking in the formation of co-continuous-like morphology in polylactide/poly(ether)urethane blends

Polymer (2015)

Hao Xiu Yan Zhou Chunmei Huang Hongwei Bai Qin Zhang

Morphology

10.1016/j.polymer.2015.10.034

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Citations (47)

A modular approach to the engineering of a centimeter-sized bone tissue construct with human amniotic mesenchymal stem cells-laden microcarriers

Biomaterials (2011)

Maiqin Chen Xiu Wang Zhaoyang Ye Yan Zhang Yan Zhou

Microcarrier

10.1016/j.biomaterials.2011.06.054

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Citations (110)

ω-6 Polyunsaturated fatty acids (linoleic acid) activate both autophagy and antioxidation in a synergistic feedback loop via TOR-dependent and TOR-independent signaling pathways

Cell Death and Disease (2020)

Bo Yang Yan Zhou Mengjiao Wu Xueshan Li Kangsen Mai

Abstract ω-6 Polyunsaturated fatty acids (PUFAs) are essential fatty acids that participate in macroautophagy (hereafter referred to as autophagy) and the Kelch ECH-associating protein 1 (Keap1)—nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant system in organisms. However, the molecular mechanisms by which ω-6 PUFAs (linoleic acid) regulate autophagy and Keap1–Nrf2 antioxidant system are not completely understood. Therefore, the purposes of this study were to explore the molecular mechanisms by which ω-6 PUFAs (linoleic acid) regulate autophagy and antioxidant system and to investigate the potential relationship between autophagy and antioxidant system through transcriptomic analysis, quantitative real-time polymerase chain reaction (RT-qPCR), western blot analysis, coimmunoprecipitation (Co-IP) and electrophoretic mobility shift assays (EMSAs) in vivo and in vitro. The results of the present study indicated that ω-6 PUFAs in diets induced autophagy but decrease antioxidant ability in vivo. However, the results also provided evidence, for the first time, that ω-6 PUFAs (linoleic acid) induced autophagy and increased antioxidant ability through the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway and the AMPK-target of rapamycin (TOR) signaling pathway in hepatocytes in vitro. Interestingly, the findings revealed a ω-6 PUFA-induced synergistic feedback loop between autophagy and antioxidant system, which are connected with each other through the P62 and Keap1 complex. These results suggested that ω-6 PUFAs (linoleic acid) could be useful for activating a synergistic feedback loop between autophagy and antioxidant system and could greatly aid in the prevention and treatment of multiple pathologies.

TOR signaling

10.1038/s41419-020-02750-0

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Citations (68)

Property Research of Silk Fibroin Nanofibers by Electrospinning Dissolved in CaCl<sub>2</sub>-Formid Acid

Advanced materials research (2015)

Yan Zhou Hui Ying Wu

In this paper, property research of silk fibroin nanofibers by electrospinning dissolved in CaCl 2 -Formid Acid was discussed. The dissolve process, morphology, structure and mechanical properties of fibers were studied. It showed that more dissolution time was needed to dissolve silk with 1% CaCl 2 . The diameters of silk fibroin nanofibers decreased with the CaCl 2 contents increased and the surface of 6%5% fibers was more uniform and smooth. After ethanol treatment, strong peak at 1628cm -1 , 1517cm -1 and 1231cm -1 was corresponding to β-sheet. It was important to discover that silk fibroin was weakly destroyed in CaCl 2 -FA solvent system, and the regenerated silk fibroin nanofibers had better mechanical properties. The stress of 6%5% fibers was nearly 3 times to traditional way. With different content of CaCl 2 , the stress of 6%5% fibers was nearly 2 times to 6%1% fibers.

Fibroin

Electrospinning

Morphology

10.4028/www.scientific.net/amr.1120-1121.331

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Citations (1)

Improvement of lung ischemia–reperfusion injury by inhibition of microRNA‐155 via reductions in neuroinflammation and oxidative stress of vagal afferent nerve

Pulmonary Circulation (2020)

Yan Zhou Lianjie Zhang Jingjing Guan Xin Yin

Lung ischemia–reperfusion injury (LIRI) is a common clinical concern. As the injury occurs, the pulmonary afferent nerves play a key role in regulating respiratory functions under pathophysiological conditions. The present study was to examine the effects of inhibiting microRNA‐155 on the levels of proinflammatory cytokines and products of oxidative stress in the pulmonary vagal afferent nerves and the commissural nucleus of the solitary tract (cNTS) after LIRI. A rat model of LIRI was used. ELISA method was employed to examine proinflammatory cytokines, namely, IL‐1β, IL‐6 and TNF‐α; and key biomarkers of oxidative stress, 8‐isoprostaglandin F2α (8‐iso PGF2α) and 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG). In results, in the process of LIRI, the levels of microRNA‐155 were amplified in the vagal afferent nerves and cNTS, and this was accompanied with increases of IL‐1β, IL‐6 and TNF‐α; and 8‐iso PGF2α and 8‐OHdG. Application of microRNA‐155 inhibitor, but not its scramble, attenuated the elevation of proinflammatory cytokines and amplification of 8‐iso PGF2α and 8‐OHdG in those nerve tissues. In conclusion, we observed the abnormalities in the pulmonary afferent pathways at the levels of the peripheral nerves and brainstem, which is likely to affect respiratory functions as LIRI occurs. Our data suggest that blocking microRNA‐155 signal pathways plays a beneficial role in regulating LIRI via inhibiting responses of neuroinflammation and oxidative stress signal pathways to LIRI.

Proinflammatory cytokine

10.1177/2045894020922125

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Citations (2)

Simultaneously reinforcing and toughening of polylactide/carbon fiber composites via adding small amount of soft poly(ether)urethane

Composites Science and Technology (2016)

Hao Xiu Xiao‐dong Qi Zhenwei Liu Yan Zhou Hongwei Bai

10.1016/j.compscitech.2016.02.025

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Citations (32)