Maryam Bozorgi

Qom University of Medical Science and Health Services

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Mozafar Khazaei

Kermanshah University of Medical Sciences

Azam Bozorgi

Kermanshah University of Medical Sciences

Mohammad Rasool Khazaei

Kermanshah University of Medical Sciences

Mansoureh Soleimani

Iran University of Medical Sciences

Zahra Jamalpoor

Aja University of Medical Sciences

Fatemeh Khazaei

Zahedan University of Medical Sciences

Azam Bozorgi Zarrini

Kermanshah University of Medical Sciences

Mohammad Taheri‬

Hamedan University of Medical Sciences

Babak Vahdatpour

Isfahan University of Medical Sciences

Ziba Rahimi

Kermanshah University of Medical Sciences

Cooperative Institutions

Kermanshah University of Medical Sciences

Tehran University of Medical Sciences

Iran University of Medical Sciences

Qom University of Medical Science and Health Services

Shahid Beheshti University of Medical Sciences

University of Tehran

Isfahan University of Medical Sciences

Tarbiat Modares University

Hamedan University of Medical Sciences

Kashan University of Medical Sciences

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Erratum to: Decellularized Extracellular Matrices in Bone Tissue Engineering: From Cells to Tissues. Mini-Review

Cell and Tissue Biology (2021)

Azam Bozorgi Zarrini Maryam Bozorgi Mozafar Khazaei Mansoureh Soleimani

Decellularization

10.1134/s1990519x21030147

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A hybrid construct of decellularized matrix and fibrin for differentiating adipose stem cells into insulin‐producing cells, an optimized in vitro assessment

Cell Biochemistry and Function (2024)

Azam Bozorgi Mohammad Rasool Khazaei Maryam Bozorgi Mozafar Khazaei

Abstract The generation of insulin‐producing cells (IPCs) is an attractive approach for replacing damaged β cells in diabetic patients. In the present work, we introduced a hybrid platform of decellularized amniotic membrane (dAM) and fibrin encapsulation for differentiating adipose tissue‐derived stem cells (ASCs) into IPCs. ASCs were isolated from healthy donors and characterized. Human AM was decellularized, and its morphology, DNA, collagen, glycosaminoglycan (GAG) contents, and biocompatibility were evaluated. ASCs were subjected to four IPC differentiation methods, and the most efficient method was selected for the experiment. ASCs were seeded onto dAM, alone or encapsulated in fibrin gel with various thrombin concentrations, and differentiated into IPCs according to a method applying serum‐free media containing 2‐mercaptoethanol, nicotinamide, and exendin‐4. PDX‐1, GLUT‐2 and insulin expression were evaluated in differentiated cells using real‐time PCR. Structural integrity and collagen and GAG contents of AM were preserved after decellularization, while DNA content was minimized. Cultivating ASCs on dAM augmented their attachment, proliferation, and viability and enhanced the expression of PDX‐1, GLUT‐2, and insulin in differentiated cells. Encapsulating ASCs in fibrin gel containing 2 mg/ml fibrinogen and 10 units/ml thrombin increased their differentiation into IPCs. dAM and fibrin gel synergistically enhanced the differentiation of ASCs into IPCs, which could be considered an appropriate strategy for replacing damaged β cells.

Decellularization

10.1002/cbf.4038

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The anti‐cancer effect of chitosan/resveratrol polymeric nanocomplex against triple‐negative breast cancer; an in vitro assessment

IET Nanobiotechnology (2022)

Azam Bozorgi Zahra Ojaghi Haghighi Mohammad Rasool Khazaei Maryam Bozorgi Mozafar Khazaei

Herein, the authors synthesised chitosan nanoparticles (Cs NPs) as a resveratrol (RSV) carrier and evaluated their efficacy in stimulating apoptosis in MDA-MB 231 cells. Blank (Cs NPs) and RSV- Cs NPs (RSV-Cs NPs) were synthesised via ionic gelation and characterised by using fourier-transform infrared spectroscopy (FTIR), Scanning electron microscope, dynamic light scattering/Zeta potential and RSV release. MDA-MB 231 cells were treated with RSV, Cs NPs and RSV-Cs NPs (24, 48, and 72 h), followed by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Cell toxicity was evaluated using lactate dehydrogenase assay, and real-time polymerase chain reaction was performed to explore apoptosis induction. FTIR spectra confirmed the NPs via the formation of cross-linking bonds. Cs and RSV-Cs NPs sizes were about 75 and 198 nm with 14 and 24 mV zeta potentials. The RSV entrapment efficiency was 52.34 ± 0.16%, with an early rapid release followed by a sustained manner. Cs and RSV-Cs NPs inhibited cell proliferation at lower concentrations and IC50 values. RSV-Cs NPs had the most cytotoxic effect and stimulated intrinsic apoptotic pathway, indicated by increased Bcl-2-associated x (BAX), BAX/Bcl-2 ratio, P53 expressions, reduced Bcl-2 and upregulated caspases 3, 8 and 9. RSV-Cs NPs have a great potential to suppress invasive breast cancer cell proliferation by targeting mitochondrial metabolism and inducing the intrinsic apoptotic pathway.

Zeta potential

HeLa

10.1049/nbt2.12108

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Immunotherapy and immunoengineering for breast cancer; a comprehensive insight into CAR-T cell therapy advancements, challenges and prospects

Cellular Oncology (2022)

Azam Bozorgi Maryam Bozorgi Mozafar Khazaei

Cell therapy

Cancer Immunotherapy

10.1007/s13402-022-00700-w

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In Vitro Stem Cell Isolation from Human Breast Cancer

DOAJ (DOAJ: Directory of Open Access Journals) (2021)

Azam Bozorgi Fatemeh Khazaei Maryam Bozorgi Mozafar Khazaei

Background: Breast cancer (BC) is the most prevalent cancer among women worldwide with significant incidence and death rates. Nowadays, researchers hold that tumor formation, failure in therapy, and disease progression are all related to the presence of a small fraction of cancer cells with self-renewal capability known as “breast cancer stem cells” (BCSCs). Therefore, the study of this cancer cell population can be conducive to eradicating the tumor. The objective of the present study was to survey the existence and in vitro isolation of human BCSCs. Method: An in vitro research study was conducted under controlled laboratory settings to isolate, enrich, and identify breast cancer stem cells. Briefly, fresh breast tumors were carried to the lab immediately after surgery, followed by mechanical and enzymatic digestion (2 mg/ml collagenase I). Then, digested samples were passed through cell strainers (70 and 40 μm), and obtained cell suspension was cultured under the serum-free medium supplemented with growth factors for 21 days. The expression of cluster of differentiation 44 (CD44) and cluster of differentiation 24 (CD24) surface markers was assessed using immunocytochemistry, and stem cell gene expression was analyzed via RT-PCR. Results: BCSCs were able to survive in serum-free conditions and form floating spheres in vitro. Cells obtained from mammospheres expressed CD44 as the membranous and cytoplasmic pattern while CD24 expression was negative. Also, octameter-binding transcription factor 4 and SOX2 gene expression was observed in BCSCs. Conclusion: The presence of stem cells was confirmed in Iranian women BC, and an efficient in vitro mammosphere culture model was used to enrich and propagate BCSCs. In our opinion, this in vitro model could be a suitable method for isolating and enriching BCSCs.

Isolation

10.30476/mejc.2021.83672.1181

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MTHFR C677T Polymorphism Is Associated with the Risk of Breast Cancer among Kurdish Population from Western Iran

International Journal of Cancer Management (2019)

Zohreh Rahimi Maryam Bozorgi Ziba Rahimi Ebrahim Shakiba Kheirollah Yari

Background: The key enzyme of methylenetetrahydrofolate reductase (MTHFR) is involved in DNA biosynthesis and repair. Objectives: The role of MTHFR C677T polymorphism in susceptibility to breast cancer is controversial. Methods: In the present case-control investigation, 297 individuals consisted of 100 patients with breast cancer and 197 healthy women were studied for MTHFR C677T genotypes, using PCR-RFLP method. Results: The frequency of MTHFR TT genotype was 10% in patients and 3% in controls (P = 0.008). The presence of TT genotype was associated with susceptibility to breast cancer [OR = 1.97, 95%CI: 1.16-3.36, P = 0.012]. The T allele of MTHFR was found in 30% of the patients compared to 27.6% healthy controls (P = 0.024) that enhanced the risk of breast cancer by 1.56 times (95% CI: 1.06 - 2.3, P = 0.024). There were 71 individuals (71%) with the age of breast cancer diagnosis ≤ 51 years old. Comparing patients with the age of cancer diagnosis ≤ 50 years old with those > 51 years old group indicated a higher frequency of MTHFR TT genotype in the latter (20.7%) compared to the first group (5.6%, P = 0.05). Conclusions: Our study demonstrated an association between the MTHFR C677T polymorphism with the risk of breast cancer among population of Western Iran. Also, our study suggests that the MTHFR TT genotype could be a risk factor for breast cancer in postmenopausal women.

10.5812/ijcm.67895

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Citations (6)

In vitro survey on the synergistic effect of Cichorium intybus L. and doxorubicin on apoptotic induction in myeloid (NALM-6) and lymphoid (KG-1) cell lines

DOAJ (DOAJ: Directory of Open Access Journals) (2022)

Maryam Bozorgi Afshin Karami Fatemeh Khazaei Mohammad Rasool Khazaei

Cichorium

10.32113/wcrj_20221_2157

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Computational and in vitro analyses on synergistic effects of paclitaxel and thymoquinone in suppressing invasive breast cancer cells

Molecular Biology Reports (2024)

Mohammad Rsool Khazaei Maryam Bozorgi Mozafar Khazaei Alireza Moradi Azam Bozorgi

Thymoquinone

IC50

MTT assay

Viability assay

10.1007/s11033-024-09328-5

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Diffuse Alveolar Hemorrhage as the initial Presentation of Systemic Lupus Erythematous Possibly Triggered by Rhogam Injection in a 24-year-old Pregnant Woman; a case report

Modern Rheumatology Case Reports (2025)

Amir Khanmirzaei Maryam Bozorgi Gelareh Azarinoush Fatemeh Aghaei AK Bigdeli

Abstract Diffuse Alveolar Hemorrhage (DAH) is an uncommon and potentially life-threatening occurrence in systemic lupus erythematous (SLE), involving bleeding into the alveolar space caused by the disruption of the alveolar-capillary basement membrane. We present a 24-year-old Persian woman with a complaint of progressively worsening shortness of breath following the administration of intramuscular-Rhogam after 3 days. According to her worsening clinical condition, the pregnancy was terminated. She was admitted to intensive care unit and intubated. Simultaneously, she developed fungal and bacterial pneumonia tolerant to therapies. After several investigations, the patient was finally diagnosed with SLE along with diffuse alveolar hemorrhage, as the first presentation of the disease. This is the first case of a pregnant woman experiencing diffuse alveolar hemorrhage as a Lupus flare following Rhogam injection. Clinicians should be aware of the mimicry nature of lupus, and the importance of immune reactions in these patients.

Diffuse alveolar hemorrhage

Presentation (obstetrics)

Diffuse alveolar damage

10.1093/mrcr/rxaf001

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The safety and efficacy of pRESET stent retriever for treatment of thrombo-embolic stroke; a systematic review and meta-analysis

The Neuroradiology Journal (2024)

Mohammad Amin Habibi Muhammad Hussain Ahmadvand Pouria Delbari S. A. Sabet Amir Zare

Background The pRESET stent retriever is a self-expanding nitinol stent designed for mechanical thrombectomy in cases of large vessel occlusion during acute ischemic stroke. This systematic review and meta-analysis synthesize the available evidence on the safety and efficacy of the pRESET device. Methods This is a systematic review and meta-analysis study conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The electronic databases of PubMed, Embase, WoS, and Scopus were systematically reviewed from inception to 8 July 2024. Results A total of eight studies involving 1163 patients were included. The pooled mortality rate was 18% with a 95% CI of [12%, 25%]. The rates of any hemorrhagic complication, parenchymal hemorrhage, and subarachnoid hemorrhage were 22% with a 95% CI of [12%, 36%], 7% with a 95% CI of [4%, 13%], and 10% with a 95% CI of [5%, 17%], respectively. The rate of favorable functional outcome (modified Rankin Scale 0-2) at 90 days was 43% with a 95% CI of [34%, 52%]. Successful recanalization rates were 60% with a 95% CI of [52%, 67%] after the first pass and 90% with a 95% CI of [83%, 95%] after the final pass. Rescue devices were used in 13% with a 95% CI of [7%, 24%] of cases. Conclusions The pRESET stent retriever demonstrates high recanalization rates and reasonable safety outcomes in patients undergoing mechanical thrombectomy for acute ischemic stroke due to large vessel occlusion. Further randomized trials directly comparing pRESET to other stent retrievers are warranted.

Stroke

10.1177/19714009241303083

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