Débora de Souza Collares Maia Castelo Branco

Universidade Federal do Ceará

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Marcos Fábio Gadelha Rocha

Universidade Estadual do Ceará

Rossana de Aguiar Cordeiro

Universidade Federal do Ceará

José Júlio Costa Sidrim

Universidade Federal do Ceará

Mariana Lara Mendes Pergentino

Universidade Federal do Ceará

Ana Luiza Ribeiro Aguiar

Universidade Federal do Ceará

Fernando Victor Monteiro Portela

Administração Regional de Saúde de Lisboa e Vale do Tejo

Wanessa Fernandes Matias Regis

Universidade Federal do Ceará

Raimunda Sâmia Nogueira Brilhante

Universidade Federal do Ceará

Maria Gleiciane da Rocha

Universidade Estadual do Ceará

Bruno Rocha Amando

Universidade Federal do Ceará

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Universidade Federal do Ceará

Universidade Estadual do Ceará

Instituto Federal de Educação, Ciência e Tecnologia do Ceará

Secretaria da Saúde

Children's Specialized Hospital

Universidade de Fortaleza

Unichristus

Instituto Federal do Amazonas

Administração Regional de Saúde de Lisboa e Vale do Tejo

University of Coimbra

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Heterologous extracellular DNA facilitates the development of Trichosporon asahii and T. inkin biofilms and enhances their tolerance to antifungals

Biofouling (2022)

Lívia Maria Galdino Pereira Ana Raquel Colares de Andrade Fernando Victor Monteiro Portela Ana Luiza Ribeiro Aguiar Bruno Nascimento da Silva

Trichosporon asahii and T. inkin are emergent agents of deep-seated and disseminated infections in immunocompromised patients. The present study aimed to investigate the role of extracellular DNA (eDNA) and the enzyme deoxyribonuclease (DNase) on the structure of T. asahii and T. inkin biofilms, as well as to examine their effect on the susceptibility to antifungals. Biofilms reached maturity at 48 h; eDNA concentration in the supernatant increased over time (6 < 24 h < 48h). Exogenous eDNA increased biomass of Trichosporon biofilms at all stages of development, enhanced their tolerance to antifungals and improved their structural complexity. DNase reduced biomass, biovolume and thickness of Trichosporon biofilms, thereby rendering them more susceptibility to voriconazole. The results suggest the relevance of eDNA in the structure and antifungal susceptibility of Trichosporon biofilms and highlight the potential of DNase as adjuvant in biofilm control.

Trichosporon

10.1080/08927014.2022.2130788

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Combination of Phenotypic Tests as a Screening Approach for the Differentiation of Cryptic Species Candida albicans and Candida dubliniensis

Medical Mycology Open Access (2017)

Marcos Fábio Gadelha Rocha Silviane Praciano Bandeira Lucas Pereira de Alencar Gláucia Morgana de Melo Guedes Débora de Souza Collares Maia Castelo Branco

Background: Candida albicans, an important pathogen for humans and animals, shares phenotypic features with Candida dubliniensis, leading to misidentification. Thus, the goal of this study was to apply a combination of phenotypic tests for the differentiation of these cryptic species. Methods and findings: Thirty-seven azole-resistant C. albicans from animals and 03 C. dubliniensis from humans were included in this study. Purity of strains was evaluated on CHROMagar Candida™, on which both species present green colonies. Then, phenotypic characterization was performed based on the growth pattern on sunflower seed agar, where C. albicans presents smooth colonies and C. dubliniensis presents rough colonies, and esterase production on Tween 80™ agar, which is positive for C. albicans, forming an opacification zone, and negative for C. dubliniensis. Molecular differentiation was performed with primers CALF/CALR for the amplification of ITS1/ITS2 regions of rDNA, yielding an amplicon of 100 bp for C. albicans, but not for C. dubliniensis. Of the 37 C. albicans, 35 showed green colonies on CHROMagar Candida Medium™, 36 presented smooth colonies on sunflower seed agar, while 34 showed an opacification zone on Tween 80™ agar. PCR yielded 100-bp-amplicons for all 37 C. albicans strains, confirming their identification. Control strains of C. dubliniensis showed the expected phenotypic features and amplicons were not obtained for the specific PCR reaction. Conclusions: The phenotypic methods used were not absolutely effective, however, the combined observation of smooth colonies on sunflower seed agar with an opacification zone on Tween 80™ agar leads to a reliable presumptive phenotypic identification of C. albicans.

Candida dubliniensis

10.21767/2471-8521.100024

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Modelo padrão de resposta a nomeação como perito médico na justiça do trabalho

17º Congresso da Associação Nacional de Medicina do Trabalho (2018)

Anisio Silvestre Pinheiro Santos Filho Wanessa Fernandes Matias Regis Crister Jose Ocadaque Bruno Rocha Amando Débora de Souza Collares Maia Castelo Branco

Introdução: Consequências tardias de eventos catastróficos e acidentes de trabalho (AT) são apontadas na literatura.Trata-se de efeitos físicos e psíquicos pós traumatismos cranianos, fraturas ósseas, amputações, intoxicações agudas, colisões de veículos, presença em acidentes fatais de colegas e outros acidentes graves.Este estudo denuncia a invisibilidade desses efeitos no Brasil.Objetivo: Destacar análises de acidentes como janelas de oportunidade na identificação dos impactos desses eventos.Método: Explorar registros de investigações apoiadas no Modelo de Análise e Prevenção de Acidentes de Trabalho (MAPA) que permitiram identificar lacunas na vigilância e assistência decorrentes dos efeitos

10.5327/z16794435201917s1tl127

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Leveduras da microbiota de anfíbios e répteis do bioma Caatinga no Ceará, Nordeste do Brasil: alto potencial patogênico de Candida famata

Ciência Rural (2021)

Débora de Souza Collares Maia Castelo Branco Renan Vasconcelos da Graça Filho Jonathas Sales de Oliveira Maria Gleiciane da Rocha Géssica dos Santos Araújo

EnglishStudies on the fungal microbiota of reptiles and amphibians are necessary to better understand of host-microbe interactions and the establishment of fungal disease in these animals. However, these studies are limited. The present researchidentified yeasts from free-ranging reptiles and amphibians from the Caatinga biome andevaluated the virulence factors production, the antifungal susceptibility in planktonic and biofilm growth and the pathogenicity of Candida famata isolates. Twenty-nine isolates of the genera Candida, Cryptococcus and Rhodotorula were identified by phenotypic and/or molecular methods and production of hydrolytic enzymes in vitro by these genera of fungi was evaluated. In addition, susceptibility of planktonic cells and biofilms to azoles and amphotericin B was evaluated. The pathogenicity of C. famata, the most prevalent yeast species isolated, was evaluated using Caenorhabditis elegans model. C. famata was the most prevalent yeast in amphibian and reptilian microbiota. Phospholipase and protease production was observed in 18/29 and 11/29 of the yeast isolates, respectively, while 100% formed biofilms. Itraconazole presented high minimal inhibitory concentrations against C. famata and C. tropicalis. Amphotericin B reduced the biomass and metabolic activity of biofilms. C. famata induced the mortality of C. elegans. In conclusion, reptiles and amphibians are colonized by yeasts capable of producing important virulence factors, especially by Candida spp. that present low susceptibility to azoles which may result from imbalances in ecosystem. Finally, C. famata isolated from these animals presented high pathogenicity, showing the importance of the study of reptile and amphibians fungal microbiota portuguesEstudos sobre a microbiota fungica de repteis e anfibios sao necessarios para melhor compreender as interacoes hospedeiro-microrganismo e o estabelecimento de doencas fungicas nesses animais. No entanto, esses estudos sao limitados. O objetivo da presente pesquisa foi identificar leveduras isoladas de repteis e anfibios do bioma Caatinga e avaliar a producao de fatores de virulencia, a sensibilidade a antifungicos no crescimento planctonico e de biofilme e a patogenicidade de Candida famata. Vinte e nove isolados dos generos Candida, Cryptococcus e Rhodotorula foram identificados por metodos fenotipicos e/ou moleculares e a producao de enzimas hidroliticas in vitro por esses generos de fungos foi avaliada. Alem disso, foi avaliada a suscetibilidade de celulas planctonicas e biofilmes a azolicos e anfotericina B. A patogenicidade de C. famata, a especie de levedura isolada mais prevalente, foi avaliada usando Caenorhabditis elegans. C. famata foi a levedura mais prevalente na microbiota de anfibios e repteis. A producao de fosfolipase e protease foi observada em 18/29 e 11/29 dos isolados de levedura, respectivamente, enquanto 100% formaram biofilmes. O itraconazol apresentou altas concentracoes inibitorias minimas contra C. famata e C. tropicalis. A anfotericina B reduziu a biomassa e atividade metabolica dos biofilmes. C. famata induziu a mortalidade de C. elegans. Em conclusao, repteis e anfibios sao colonizados por leveduras capazes de produzir importantes fatores de virulencia, especialmente por cepas de Candida spp. que apresentam baixa suscetibilidade a azolicos que podem resultar de desequilibrio no ecossistema. Por fim, C. famata isolados desses animais apresentaram alta patogenicidade, mostrando a importância do estudo da microbiota fungica de repteis e anfibios

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