Sreeramanan Subramanıam

Universiti Sains Malaysia

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Bee Lynn Chew

Universiti Sains Malaysia

Ranjetta Poobathy

Quest International University Perak

Uma Rani Sinniah

Universiti Putra Malaysia

Subash C. B. Gopinath

Universiti Malaysia Perlis

Maziah Mahmood

Western University

Jessica Jeyanthi James Antony

Universiti Putra Malaysia

Jasim Uddain

Sher-e-Bangla Agricultural University

Suganthi Appalasamy

Universiti Malaysia Kelantan

Chan Lai Keng

Hospital Universiti Sains Malaysia

Xavier Rathinam

AIMST University

Cooperative Institutions

Universiti Sains Malaysia

167

AIMST University

Universiti Putra Malaysia

Hospital Universiti Sains Malaysia

Universiti Malaysia Perlis

University of Malaya

Malaysian Agricultural Research and Development Institute

Tamil Nadu Agricultural University

National University of Malaysia

Airlangga University

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Current Scenario in Associating Clinical COVID-19 Biomarkers for Developing Surveillance Platforms

Current Medicinal Chemistry (2025)

Thangavel Lakshmipriya Subash C. B. Gopinath Yeng Chen Sreenivasan Sasidharan Sreeramanan Subramanıam

Abstract: The novel coronavirus that caused the epidemic and pandemic resulting in the acute respiratory illness known as coronavirus disease 2019 (COVID-19) has plagued the world. This is unlike other coronavirus outbreaks that have occurred in the past, such as Middle East respiratory syndrome (MERS) or severe acute respiratory syndrome (SARS). COVID-19 has spread more quickly and posed special challenges due to the lack of appropriate treatments and vaccines. Real-time polymerase chain reaction (RTPCR) and rapid antibody tests (surveillance tests) are the two most used tests (confirmation tests). However, the latter takes hours to complete, and the former may produce false positives. Scientists have invested significant effort to create a COVID-19 diagnostic system that is both highly sensitive and reasonably priced. Early detection of COVID-19 is a major area of focus for sensing devices based on nanomaterials. This overview enhanced insights into potential coronavirus biomarkers and, compared to earlier studies, introduced new avenues. Further, it covers the development of COVID-19 diagnostic systems from an analytical point of view, including clinical markers and their subsequent applications with biosensors.

Coronavirus

Pandemic

2019-20 coronavirus outbreak

Gold standard (test)

10.2174/0109298673331044241031100627

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Preliminary study on the effects of fusaric acid treated protocorm-like bodies of Dendrobium hybrid against Fusarium proliferatum and Fusarium oxysporum

Malaysian Journal of Microbiology (2019)

Sangeetha Siva Sangu Nik Mohd Izham Mohamed Nor Latiffah Zakaria Azhar Mohamad Sreeramanan Subramanıam

Aims: Dendrobiums are majorly affected by Fusarium proliferatum and F. oxysporum.The aim of this research was to utilise the mycotoxin, fusaric acid (FA) on Dendrobium hybrid to produce cultivars that are resistant towards these fungi.Methodology and results: FA of concentrations 0.05, 0.10, 0.15 and 0.20 mM were transferred to sterilised halfstrength Murashige and Skoog (MS) medium and inoculated with four weeks old thin cell layer (TCL) of protocorm-like bodies (PLBs) for eight weeks.It was deduced that PLBs treated with 0.10 mM of FA resulted in highest survival and shoot regeneration rate but the survival and regeneration rate began to decline as the concentrations of FA were increased.Histology and scanning electron microscopy (SEM) observation showed prominent cell damage and stomatal closure in PLBs treated with FA.Direct amplification of minisatellite DNA (DAMD) markers showed polymorphism in the FA treated PLBs compared to the control PLBs.In the leaf bridge bioassay, plantlets treated with 0.05 mM of FA showed most resistance towards both fungal species.Conclusion, significance and impact of study: Therefore, this research is a preliminary screening study where the optimum concentration of FA was selected based on the reaction of treated TCL of PLBs towards these mutagens.

Fusaric acid

Fusarium proliferatum

Dendrobium

Beauvericin

10.21161/mjm.180109

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Antimicrobial Activity and Toxicity of Punica granatum L., Peel

Journal of Applied Biological Sciences (2008)

Mehru Nisha Xavier Rathinam Sreeramanan Subramanıam A. Rajasekaran Sreenivasan Sasidharan

Methanols extract of the Punica granatum L. (Punicaceae) peel was screened for antimicrobial activity against enteric pathogenic bacteria. Antimicrobial tests were carried out using the disk diffusion assay and broth dilution method. The extract showed favorable antimicrobial activity against enteric pathogenic bacteria tested with a minimum inhibitory concentration (MIC) value of 12.50 mg/ml. Furthermore, the extract was also tested against brine shrimp for toxicity. The extract exhibited no signifi cant toxicity (LC 50 = 1.42 mg/ml) against Artemia salina. The study confi rms the possible antimicrobial potentiality of the peel extract of P. granatum. The study shows that the extract is a favorable antimicrobial agent with potential applications in public health against diseases.

Punica

Artemia salina

Brine shrimp

Pathogenic bacteria

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Citations (10)

Zero-dimensional gold application in colorimetrically optimized ELISA signal enhancement for diagnosing osteoarthritis

Process Biochemistry (2022)

Peng Li Bo Zhang Subash C. B. Gopinath Sreeramanan Subramanıam Jin Zhang

N-terminal telopeptide

10.1016/j.procbio.2022.09.002

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Effects of Coconut Water and Banana Homogenate on Shoot Regeneration of Meyer Lemon (Citrus × meyeri)

Pertanika journal of tropical agricultural science (2024)

Stephanie Qiao Er Wong Najwa Amalina Haradzi Dahmendra Sriskanda Sreeramanan Subramanıam Bee Lynn Chew

Meyer lemon (Citrus × meyeri) is a hybrid citrus fruit from the Rutaceae family, originating from China. It is well-known for its distinctive appearance and flavor, as well as its health-nourishing nutrients. Micropropagation is an efficient alternative in the multiplication of plant stocks suitable for the commercial scale. The inclusion of organic additives in culture media has been found to provide a cost-effective option as a plant growth stimulant for in vitro plant development. The current study intends to assess the effects of coconut water and banana homogenate in the regeneration of Meyer lemon. In vitro, shoots were treated in half-strength Murashige and Skoog media fortified with 2 mg/L 6-benzylaminopurine with varying concentrations of coconut water and banana homogenate without sucrose. Results revealed that the treatment of 30% coconut water and 40 g/L banana homogenate resulted in the greatest proliferation of new shoots (3.00 ± 0.873 and 1.57 ± 0.297, respectively), whereas treatment of 40% coconut water resulted in the greatest shoot elongation of 0.239 ± 0.026 cm. The current study suggested the incorporation of coconut water and banana homogenate as potential substitutes for carbon sources and growth stimulants in the regeneration of Meyer lemon.

Citrus × sinensis

Rough lemon

Citrus limon

10.47836/pjtas.47.1.11

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Test tube bouquets - in vitro flowering.

Sreeja Sudhakaran J. A. T. da Silva Sreeramanan Subramanıam J. A. Teixeira da Silva

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Citations (12)

Vitrification and histological analyses of protocorm-like bodies of 'vanda' kaseem's delight orchid

Australian Journal of Crop Science (2012)

Ranjetta Poobathy Nor'Ain Ahmad Hesam Advina Lizah Julkifle Sreeramanan Subramanıam

This study was conducted to investigate the potential of cryopreserving protocorm-like bodies (PLBs) of Vanda Kaseem's Delight Orchid using plant vitrification solution 2 (PVS2). Measured parameters included the effect of PLB size (1-2 and 3-4mm), the effect of sucrose preculture concentrations and duration (0.0, 0.10, 0.25, 0.50 and 0.75 M, for 24 and 48 hours), the effect of dehydration duration (0, 10, 20, 30 and 40 minutes) and the effect of various unloading periods (0, 10, 20, 30 and 40 minutes) on survival of cryopreserved PLBs, as assessed using spectrophotometric 2,3,5-triphenyltetrazolium chloride (TTC) assay at 490nm. The PLBs were also subjected to a histological study to observe differences in both cryopreserved and non-cryopreserved PLBs of the orchid. The best results in the cryopreservation of PLBs of Vanda Kaseem's Delight was obtained when 3-4mm PLBs were precultured in VW medium supplemented with 0.1M sucrose for 24 hours, followed by a loading treatment, and 20 minutes of dehydration in PVS2 at 0 degreesC, prior to cryostorage, and 30 minutes of unloading treatment after 90 seconds of thawing. Histological observations of cryopreserved PLBs indicated that most of the damages resulting from cryostorage occurred in the cell wall and nucleus of the cells.

Vitrification

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Citations (11)

Antibacterial and Antifungal Agents of Higher Plants

Springer eBooks (2019)

Balasupramaniam Kirubakari Shanmugapriya Shanmugapriya T. Sangeetha Soundararajan Vijayarathna Yeng Chen

10.1007/978-981-13-7154-7_16

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Citations (7)

Transfer of β-1,3-glucanase gene into banana for tolerance to Fusarium wilt disease race 1 using Agrobacterium-mediated transformation system

Sreeramanan Subramanıam Maziah Mahmood Xavier Rathinam

Agrobacterium-mediated transformation of single buds in vitro grown banana cv. ‘Rastali’ (AAB) was done using the binary vector pROKla-Eg, harboring the soybean endo β-1,3-glucanase gene (Eg). Plasmid pROKla-Eg contained a neomycin phosphotransferase gene (nptII) as the selectable marker to identify the transformants. Treatment A contained kanamycin (kan) at 100 mgl-1 and treatment B contained geneticin G-418 at 50 mgl-1 in both MS medium supplemented 5 mgl-1 of BAP together with 2.7 g of gelrite agar. Single buds derived from multiple bud clumps, were the target explants for transformation. An assay was performed to identify the minimum concentration required for two antibiotics (carbenicillin and cefotaxime) that was most effective against Agrobacterium strains, LBA 4404, and the effect on tissue regeneration capacity. Even though the transformation frequency based on kan selection medium (treatment A) was higher, no transformants could be confirmed based on PCR and Southern blot analyses, compared to the use of geneticin (G-418) selection medium (treatment B). These results suggested that the use of G-418 as a selection agent is preferable to kan due to the lower concentration required to allow for the small numbers of putative transgenic cells in a large population of non–transformed ones to undergo multiplication and also reduced the occurrence of chimeras. The transgenic banana plantlets were inoculated with 2 × 106 spores ml-1 conidial suspension of Fusarium oxysporum f. sp. cubense (race 1) to evaluate the degree of tolerance and to investigate the effectiveness of the bioassay system as a potential tool for early screening. An assay of protein extract from the transgenic plantlets showed a significant increase in EG enzyme activity over the untransformed plantlets. The present Agrobacterium-mediated transformation protocol reported here is suitable for the use of tiny meristem tissues to obtain fungal disease tolerant or resistant banana through genetic engineering.

Kanamycin

Selectable marker

Carbenicillin

Transformation efficiency

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Waterlogging stress mechanism and membrane transporters in soybean (Glycine max (L.) Merr.)

Plant Physiology and Biochemistry (2025)

Ambika Rajendran Ayyagari Ramlal Amooru Harika Sreeramanan Subramanıam Dhandapani Raju

Waterlogging (archaeology)

10.1016/j.plaphy.2025.109579

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