Aissata Boubakar Cisse

Institut National de Recherche en Santé Publique

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Bassirou Diarra

Université des Sciences, des Techniques et des Technologies de Bamako

Bouke C. de Jong

Sorbonne Université

Bocar Baya

University of Bamako

Y. Toloba

University of Bamako

Yéya dit Sadio Sarro

Université des Sciences, des Techniques et des Technologies de Bamako

M. Sanogo

Université des Sciences, des Techniques et des Technologies de Bamako

Souleymane Diallo

Université Nangui Abrogoua

Robert L. Murphy

Northwestern University

Antieme Combo Georges Togo

Université des Sciences, des Techniques et des Technologies de Bamako

Sounkalo Dao

University of Bamako

Cooperative Institutions

Université des Sciences, des Techniques et des Technologies de Bamako

University of Bamako

Mali-Folkecenter

Hôpital Aristide Le Dantec

Cheikh Anta Diop University

Instituut voor Tropische Geneeskunde

Institut National de Recherche en Santé Publique

National Institutes of Health

Ministère de la Santé et de l'Hygiène Publique

Universidade Federal de Lavras

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Identification des souches de mycobactéries isolées au Laboratoire National de Référence (LNR) de la Tuberculose du Mali : valeur diagnostique du test antigénique de diagnostic rapide SD Bioline MPT64

Aissata Boubakar Cisse F Camara Bassirou Diarra Seydou Koné K. Ouattara

Introduction: The distinction between Mycobacterium tuberculosis complex (MTBC) and Non tuberculous Mycobacteria (NTM) infection is essential for an appropriate treatment. Unfortunately this distinction is not done at regular basis in resource limited settings, as the decision to start is based on smear microscopy which cannot distinguish between bacterial species. SD Bioline rapid test for the detection of Ag MPT64 which is specific to Mycobacterium can be an interesting alternative for differential diagnosis. Objective: To identify MTBC strains isolated at the National Reference Laboratory (NRL) of tuberculosis, Bamako using Ag MPT64 immunochromatographic SD Bioline rapid test relative to traditional biochemical and molecular tests. Methods: We detected MPT64 proteins from both human and animal mycobacterial strains. Human strains were isolated from patients who were referred to NRL for culture and drug susceptibility testing (DST). SD Bioline rapid test results were confirmed using traditional biochemical tests, morphological and molecular techniques. Results: We found that SD Bioline rapid test has a specificity and sensitivity of respectively 100% and 99%. There was only one false negative (FN) case which was identified by traditional biochemical and morphological techniques. Conclusion: This is a simple technique, and may provide a fast, cheap and easy-to-perform alternative method to distinguish between mycobacterial strains in resource limited settings. However, negative results should be confirmed by another identification method such as morphological identification.

10.53597/remim.vi1.343

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Apport du GeneXpert dans le diagnostic de la tuberculose et de la résistance à la rifampicine au Mali après 22 mois d’acquisition

Revue des Maladies Respiratoires (2017)

Y. Toloba Aissata Boubakar Cisse D. Soumaré K. Ouattara T. Kanouté

10.1016/j.rmr.2016.10.515

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Prevalence of non-tuberculous mycobacteria among people presumed to have tuberculosis, positive for acid-fast bacilli in Mali

medRxiv (Cold Spring Harbor Laboratory) (2024)

Aissata Boubakar Cisse Anna Dean Armand Van Deun Jelle Keysers Willem-Bram De Rijke

Abstract Background Non-tuberculosis mycobacteria (NTMs) are environmental agents that can cause opportunistic pulmonary disease in humans and animals which is often misdiagnosed as tuberculosis (TB). In this study, we describe the cases of NTMs identified during the first national anti-TB drug-resistance survey conducted in Mali, and associated risk factors. Methods Sputum was collected from people presenting for pulmonary TB diagnosis, from April to December 2019, regardless of age. Microscopy-positive patients were enrolled and were tested by GeneXpert MTB/RIF. Cases that tested negative for the Mycobacterium tuberculosis complex (MTBc) were tested for presence of mycobacteria by amplification of the IS 6110 and 16SrRNA genes through double quantitative real-time PCR, followed by nested PCR and Sanger sequencing of the IS 6110 -negative samples for NTM species identification. Results A total of 1,418 sputum smear-positive patients were enrolled, including 1,199 new cases, 211 previously treated cases and 8 whose previous treatment history was unknown. Based on the results of GeneXpert MTB/RIF and in-house PCR methods, 1331 (93.9%) patients were positive for MTBc, 48 (3.4%) for NTMs and for 39 (2.7%) no species identification was possible. Advanced age (65 and over) (OR 8.8, p=0.001) and previous TB treatment (OR 3.4 and p=0.016) were the risk factors statistically associated with NTM detection. M. avium complex (MAC) was the predominant NTM species, detected in 20 cases. Conclusion Detection of NTMs in people presumed to have TB is an ongoing challenge, confounding correct TB diagnosis. Concomitant use of microscopy and GeneXpert testing among at-risk individuals could lessen confusion.

10.1101/2024.04.16.24304822

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Screening new tuberculosis patients in Mali for rifampicin resistance at 2months

International Journal of Mycobacteriology (2016)

Bassirou Diarra Aissata Boubakar Cisse Ousmane Kodio M. Sanogo Bocar Baya

The recent call for universal drug susceptibility testing (DST) for all tuberculosis (TB) patients will be difficult to meet in settings where Xpert rollout is limited, such as low prevalence of HIV and Multi-drug Resistant Tuberculosis (MDR) settings. As recommended by World Health Organization (WHO) guidelines, the success of TB treatment is measured by Ziehl–Neelsen (ZN) microscopy or auramine–rhodamine fluorescent microscopy (FM) on sputum, in which conversion to negative smear at 2 months (M) is an important predictor of treatment success, defined as a negative smear at 5 M. The sputum smear that fails to convert to negative at 5 M are screened for rifampicin resistance. We tested in a prospective study whether an early screen for rifampicin resistance, based on FM results at 2 M, could detect MDR patients early, rather than screening all patients with GeneXpert MTB/Rif at baseline. Between February 2015 and August 2016, we enrolled new TB patients in an IRB-approved prospective cohort study at four health centers in Bamako district. Fresh sputum samples were collected at 2 M and 5 M to measure FM smear conversion. Patients who failed to show a decline in FM positivity at 2 M (moderate or many Acid Fast Bacilli (AFB)) had their sputum tested in GeneXpert to detect rifampicin resistance. Patients who had any AFB seen at 5 M were also tested using GeneXpert. Of the 570 patients who were enrolled in the study, 22 (3.8%) died and 27 (4.7%) were lost to follow-up. The prevalence of HIV and TB coinfection was 12.4%, and 65.6% of the patients were male. At 2 M, 32 out of 429 patients still had moderate or many AFBs in FM, and were screened by Xpert, of whom 5 (15.6%) tested rifampicin-resistant and were referred for MDR treatment. Of the 310 patients who completed 5 M of treatment, 35 (11.3%) met the definition of failure (few or moderate AFB in FM) and had their sputum tested in Xpert; moreover, four (11.4%) demonstrated rifampicin resistance. In total, 67 (21.6% of 310) patients were screened by Xpert, of whom nine were detected to have MDR (or 13.4% of those screened). Although we cannot exclude additional MDR patients having been missed by our screening strategy, our screening algorithm at 2 M detected five out of nine MDR patients. Detecting patients at 2 M allowed for earlier referral, and potentially less acquired drug resistance and lower mortality. This strategy may be advantageous while awaiting further rollout of Xpert machines that will permit universal DST.

GeneXpert MTB/RIF

10.1016/j.ijmyco.2016.09.052

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Généralisation des Centres d'Education pour le Développement au Mali

Editions universitaires europeennes eBooks (2017)

Aissata Boubakar Cisse

Lors d'un stage a l'academie d'Enseignement de Mopti, j'ai ete interpellee par la problematique des centres d'education pour le developpement. L'objectif de mon etude etait d’evaluer et d'analyser les resultats et les realisations obtenus apres huit annees de mise en œuvre des Centres, d’identifier les problemes qu'ils rencontrent dans leur fonctionnement et de proposer des pistes de reflexion pour leur amelioration. J'ai effectue des enquetes de terrain pendants 12 mois en deux phases en 2010-2011 avec un echantillon de trente cinq localites reparties sur tout le territoire du Mali. Je me suis entretenue avec l'ensemble des intervenants dans les questions des CED. En reponse a la question de recherche, il est ressorti que les CED sont loin de repondre aux attentes formulees: les infrastructures et les ressources humaines sont inadaptees, les criteres de recrutement des apprenants non respectes ... Cependant, les perspectives en matiere d’education non formelle en general, et de CED en particulier sont assez prometteuses malgre les difficultes enregistrees.

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Diabetes Mellitus among new tuberculosis patients in Bamako, Mali

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases (2019)

Bassirou Diarra Mohamed Tolofoudié Yéya dit Sadio Sarro Antieme Combo Georges Togo Sidy Bane

Diabetes Mellitus (DM) increases worldwide, mostly in low- and middle-income countries. In Mali, the prevalence in the adult population is estimated at 1.8%, but tuberculosis (TB) patients are not systematically screened. The goal of our study was to determine the prevalence of DM among newly diagnosed TB patients.We conducted a cross sectional study and a pilot prospective cohort study in four health centers in Bamako. All patients underwent fasting capillary-blood glucose (FCBG) test at Day 0, and repeated after one-week of TB treatment. Venous FBG test was performed for discrepancies between the two FCBG results. Thereafter, FCBG was performed for pilot study at month-2 (M2) and M5 of TB treatment.Two hundred and one patients were enrolled in this study. Impaired fasting blood glucose was identified in 17 (8.5%), of whom 11 (5.5%) had DM (VFBG >7 mmol/L). Among patients with DM, seven (63.6%) had successful TB treatment outcome, versus 142 (74.7%) of those without DM (p = 0.64), and (OR: 1.69, 95%CI 0.47-6.02).The prevalence of DM among TB patients in Bamako exceeds that of the general population and screening at TB diagnosis suffices to identify those with DM. Systematic screening of both diseases will allow better treatment.

10.1016/j.jctube.2019.100128

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Pre-eclampsia as a predictor of early-onset cardiovascular impairment among young women (PREECARDIA study): protocol for a prospective cohort study

BMJ Open (2024)

Ruben Turé Albertino Damasceno Amabélia Rodrigues Eunice Barai Aissata Boubakar Cisse

Introduction Pre-eclampsia is a pregnancy-related complication estimated to affect up to 8% of pregnancies worldwide. It is associated with an increased risk of postpartum sustained hypertension, coronary artery disease, cerebrovascular disease, peripheral arterial disease and cardiovascular-related mortality. Nevertheless, these associations have seldom been addressed in younger women from sub-Saharan Africa (SSA). Hence, this study aims to assess the association between pre-eclampsia and cardiovascular impairment within the first year after delivery, among younger women in SSA. Methods and analysis This is a prospective cohort study conducted at Hospital Nacional Simão Mendes in Bissau, Guinea-Bissau. A total of 230 pregnant women aged below 25 years (115 diagnosed with pre-eclampsia and 115 normotensive age-matched pregnancies), will be enrolled after their 20th gestational week. Exclusion criteria include diabetes, hypertension or other serious maternal diseases present before pregnancy. Participants will be assessed at baseline (pre-labour period), 4 and 12 months after delivery; evaluations started in March 2023 and are expected to end in December 2026. At each follow-up assessment, the women will have their blood pressure measured with a digital sphygmomanometer and a 24-hour ambulatory blood pressure monitor. Cardiac and renal function will be assessed using echocardiography and laboratory testing, respectively. Primary outcomes include the mean differences in cardiovascular and renal parameters between women who had pre-eclampsia and those who had normotensive pregnancies. Age, parity, age at first pregnancy, family history of cardiovascular diseases, smoking habits, gestational diabetes diagnosed before pre-eclampsia, body mass index and labour complications will be considered a priori as potential confounders of the association between pre-eclampsia and postpartum cardiovascular impairment. Ethics and dissemination This study was approved by the Comité Nacional de Ética em Pesquisa na Saúde, Guinea-Bissau (008/CNES/INASA/2023), and participants provide written informed consent. Results will be disseminated among the scientific community and the public.

Gestational hypertension

10.1136/bmjopen-2024-091882

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Géométrie et cinématique de la déformation panafricaine majeure dans le district minier de Bleida (Bou Azzer-El Graara, Anti-Atlas central - Maroc)

Aissata Boubakar Cisse

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Mycobacterium africanum (Lineage 6) shows slower sputum smear conversion on tuberculosis treatment than Mycobacterium tuberculosis (Lineage 4) in Bamako, Mali

PLoS ONE (2018)

Bassirou Diarra Mahamadou Koné Antieme Combo Georges Togo Yéya dit Sadio Sarro Aissata Boubakar Cisse

Objective Ancestral M. tuberculosis complex lineages such as M. africanum are underrepresented among retreatment patients and those with drug resistance. To test the hypothesis that they respond faster to TB treatment, we determined the rate of smear conversion of new pulmonary tuberculosis patients in Bamako, Mali by the main MTBc lineages. Methods Between 2015 and 2017, we conducted a prospective cohort study of new smear positive pulmonary tuberculosis patients in Bamako. Confirmed MTBc isolates underwent genotyping by spoligotyping for lineage classification. Patients were followed at 1 month (M), 2M and 5M to measure smear conversion in auramine (AR) and Fluorescein DiAcetate (FDA) vital stain microscopy. Result All the first six human MTBc lineages were represented in the population, plus M. bovis in 0.8% of the patients. The most widely represented lineage was the modern Euro-American lineage (L) 4, 57%, predominantly the T family, followed by L6 (M. africanum type 2) in 22.9%. Ancestral lineages 1, 5, 6 and M. bovis combined amounted to 28.8%. Excluding 25 patients with rifampicin resistance, smear conversion, both by AR and FDA, occurred later in L6 compared to L4 (HR 0.80 (95% CI 0.66–0.97) for AR, and HR 0.81 (95%CI 0.68–0.97) for FDA). In addition we found that HIV negative status, higher BMI at day 0, and patients with smear grade at baseline ≤ 1+ were associated with earlier smear conversion. Conclusion The six major human lineages of the MTBc all circulate in Bamako. Counter to our hypothesis, we found that patients diseased with modern M. tuberculosis complex L4 respond faster to TB treatment than those with M. africanum L6.

Mycobacterium tuberculosis complex

Lineage (genetic)

10.1371/journal.pone.0208603

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SUN-427 SENEGAL RENAL BIOPSY REGISTRY: INDICATIONS AND HISTOPATHOLOGICAL PATTERNS BASED ON 1,559 NATIVE RENAL BIOPSIES

Kidney International Reports (2020)

Ahmed Tall Lemrabott Mamadou Birame Faye Mason Dial Maria Faye Aissata Boubakar Cisse

Renal biopsy (RB) is performed in few countries in sub-Saharan Africa. However, in our country, it has been practiced for a very long time with an anatomopathological reading done on site since 2009. The purpose of this study was to determine the indications of the RB, and to describe the anatomopathological and etiological aspects of biopsied nephropathies in Senegal.

Etiology

10.1016/j.ekir.2020.02.967

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