Phthalates has been widely used in children?s toys as plastic plasticizers and softeners. Therefore, attention should be paid to plastic toys, especially those that children can put in their mouths. In this paper quantification of five phthalates: DMP, DnBP, BBP, DEHP and DnOP in plastic toys, as well as irradiation of toys with UV light was performed. After sample preparation and development of the liquid?liquid phthalate extraction method from artificial saliva phthalate quantitative determination using the GC?MS technique was performed. The mean recovery value for DEHP is 77.03?2.76 %. The determination of phthalate in the recipient models (artificial saliva and n-hexane) was performed after 6, 15 and 30 days of the migration test using the GC?MS technique. Based on the known mass % DEHP in the analyzed toys, the percentage of phthalate migration from each analyzed toy to the recipient model after 6, 15 and 30 days of the migration test was calculated. The results show that there is no significant migration of DEHP into artificial saliva, due to high polarity of the recipient (artificial saliva is polar), unlike n-hexane where the migration of DEHP is significant because it is a non-polar solvent.

Plasticizer

10.2298/jsc210928097a

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Presence of beta-boldenone sulphate and glucuronide in urine of untreated beef cattle

Luca Maria Chiesa Radmila Pavlović Elisa Pasquale N. Brina P. L. Giorgi

Beef Cattle

Source

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The recent fault kinematics in the westernmost part of the Getic nappe system (Eastern Serbia): Evidence from fault slip and focal mechanism data

Geologica Carpathica (2014)

Ana Mladenović Branislav Trivić Milorad Antić Vladica Cvetković Radmila Pavlović

Abstract In this study we performed a calculation of the tectonic stress tensor based on fault slip data and all available focal mechanisms in order to determine the principal stress axes and the recent tectonic regime of the westernmost unit of the Getic nappe system (Gornjak-Ravanica Zone, Eastern Serbia). The study is based on a combined dataset involving paleostress analyses, the inversion of focal mechanisms and remote sensing. The results show dominant strike-slip kinematics with the maximal compression axis oriented NNE-SSW. This is compatible with a combined northward motion and counterclockwise rotation of the Adria plate as the controlling factor. However, the local stress field is also shown to be of great importance and is superimposed on the far-field stress. We managed to distinguish three areas with distinct seismic activity. The northern part of the research area is characterized by transtensional tectonics, possibly under the influence of the extension in the areas situated more to the northeast. The central and seismically most active part is dominated by strike-slip tectonics whereas the southern area is slightly transpressional, possibly under the influence of the rigid Moesian Platform situated to the east of the research area. The dominant active fault systems are oriented N-S (to NE-SW) and NW-SE and they occur as structures of either regional or local significance. Regional structures are active in the northern and central part of the study area, while the active fault systems in the southern part are marked as locally important. This study suggests that seismicity of this area is controlled by the release of accumulated stress at local accommodation zones which are favourably oriented in respect to the active regional stress field.

Stress field

Clockwise

Focal mechanism

10.2478/geoca-2014-0010

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Light‐Based 3D Printing of Gelatin‐Based Biomaterial Inks to Create a Physiologically Relevant In Vitro Fish Intestinal Model

Macromolecular Bioscience (2023)

Anna Szabó Rolando Pasquariello Pedro F. Costa Radmila Pavlović Indi Geurs

To provide prominent accessibility of fishmeal to the European population, the currently available, time- and cost-extensive feeding trials, which evaluate fish feed, should be replaced. The current paper reports on the development of a novel 3D culture platform, mimicking the microenvironment of the intestinal mucosa in vitro. The key requirements of the model include sufficient permeability for nutrients and medium-size marker molecules (equilibrium within 24 h), suitable mechanical properties (G' < 10 kPa), and close morphological similarity to the intestinal architecture. To enable processability with light-based 3D printing, a gelatin-methacryloyl-aminoethyl-methacrylate-based biomaterial ink is developed and combined with Tween 20 as porogen to ensure sufficient permeability. To assess the permeability properties of the hydrogels, a static diffusion setup is utilized, indicating that the hydrogel constructs are permeable for a medium size marker molecule (FITC-dextran 4 kg mol-1 ). Moreover, the mechanical evaluation through rheology evidence a physiologically relevant scaffold stiffness (G' = 4.83 ± 0.78 kPa). Digital light processing-based 3D printing of porogen-containing hydrogels results in the creation of constructs exhibiting a physiologically relevant microarchitecture as evidenced through cryo-scanning electron microscopy. Finally, the combination of the scaffolds with a novel rainbow trout (Oncorhynchus mykiss) intestinal epithelial cell line (RTdi-MI) evidence scaffold biocompatibility.

Gelatin

3D cell culture

Biomaterial

Biocompatibility

10.1002/mabi.202300016

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Quality Traits of Medical Cannabis sativa L. Inflorescences and Derived Products Based on Comprehensive Mass-Spectrometry Analytical Investigation

IntechOpen eBooks (2019)

Lorenzo Calvi Radmila Pavlović Sara Panseri Luca Giupponi Valeria Leoni

Cannabis sativa L. has been cultivated throughout the world for industrial and medical purposes and is the most controversial plant ever exploited, with considerable discrepancies in the praise and disapproval it receives. Medical Cannabis prescriptions are on the increase in several countries where its therapeutic use is authorised due to its positive role in treating several pathologies even if it represents a multifaceted reality in terms of application. There are at least 550 identified compounds in C. sativa L., including more than 100 phytocannabinoids and 120 terpenes. The chemical complexity of its bioactive constituents highlights the need for standardised and well-defined analytical approaches able to characterise plant chemotype and herbal drug quality as well as to monitor the quality of pharmaceutical cannabis extracts and preparations. This research highlights the potential of using different analytical procedures involving the combination of headspace-solid-phase microextraction (HS-SPME) coupled to GC–MS and accelerated solvent extraction (ASE) coupled to high resolution mass-spectrometry (HPLC-Q Orbitrap®) for the in-depth profiling of quality traits in authorised medical varieties of Cannabis sativa L. flos (Bediol®) and corresponding macerated oil preparations. This approach could add new knowledge to the field of "omic" analytical applications which are fundamental nowadays for Cannabis used for therapeutic remedies.

Cannabis sativa

10.5772/intechopen.79539

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Influence Of Age On Red Wine Colour During Fining With Bentonite And Gelatin

International Journal of Food Properties (2011)

Snežana Stanković Slobodan Jović Jelena Živković Radmila Pavlović

Two fining agents (bentonite and gelatin) have contributed to the reduction of colour of Pinot Noir wine of different ages. Bentonite caused more significant changes in the colour of young wines. Colour intensity, coloured anthocyanin forms, and polymers were reduced to a greater extent, while colourless anthocyanins and total phenolic substances were reduced to a lower extent. Smaller changes in coloured substances resulted from wine treatment with gelatin. The intensity of the share of yellow colour decreased and the intensity of the share of red colour, together with dA% value, increased. The influence of bentonite on the colour of the wine decreased while the influence of gelatin increased with wine ageing.

Gelatin

Bentonite

Wine color

10.1080/10942912.2010.487625

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Citations (22)