Morgan McCaw

University of Missouri

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James A. Birchler

University of Missouri

Kan Wang

Iowa State University

Nathan Swyers

University of Missouri

Changzeng Zhao

University of Missouri

Nathaniel Graham

University of Missouri

Min‐Jeong Kang

Korea Forest Service

Jon P. Cody

University of Minnesota

Jacob D. Zobrist

Iowa State University

Keunsub Lee

Iowa State University

Alan L. Eggenberger

Iowa State University

Cooperative Institutions

University of Missouri

Iowa State University

University of Georgia

Czech Academy of Sciences, Institute of Experimental Botany

Cornell University

Centre of the Region Haná for Biotechnical and Agricultural Research

Institute of Genetics and Developmental Biology

Chinese Academy of Sciences

Palacký University Olomouc

Israel Institute for Biological Research

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Handling Fast‐Flowering Mini‐Maize

Current Protocols in Plant Biology (2017)

Morgan McCaw James A. Birchler

Abstract Maize ( Zea mays ) has a long history as a model organism for genetic analysis due to its ease of crossing, relatively large number of progeny, and ability to determine many genotypic traits through phenotypic kernel markers. Fast‐Flowering Mini‐Maize A and B (FFMM) are two recently developed lines of maize selected for traits that make them more conducive to research. FFMM flowers more quickly than standard maize and is much smaller; thus it requires a slightly modified protocol for care and crossing. The following protocol describes how to plant, care for, and cross FFMM in greenhouse, growth chamber, and field conditions. © 2017 by John Wiley & Sons, Inc.

10.1002/cppb.20051

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<em>Agrobacterium</em>-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Journal of Visualized Experiments (2020)

Alicia Masters Min‐Jeong Kang Morgan McCaw Jacob D. Zobrist William Gordon‐Kamm

Demonstrated here is a detailed protocol for Agrobacterium-mediated genetic transformation of maize inbred lines using morphogenic genes Baby boom (Bbm) and Wuschel2 (Wus2). Bbm is regulated by the maize phospholipid transferase gene (Pltp) promoter, and Wus2 is under the control of a maize auxin-inducible (Axig1) promoter. An Agrobacterium strain carrying these morphogenic genes on transfer DNA (T-DNA) and extra copies of Agrobacterium virulence (vir) genes are used to infect maize immature embryo explants. Somatic embryos form on the scutella of infected embryos and can be selected by herbicide resistance and germinated into plants. A heat-activated cre/loxP recombination system built into the DNA construct allows for removal of morphogenic genes from the maize genome during an early stage of the transformation process. Transformation frequencies of approximately 14%, 4%, and 4% (numbers of independent transgenic events per 100 infected embryos) can be achieved for W22, B73, and Mo17, respectively, using this protocol.

10.3791/60782

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