Yujun Zhu

Guangdong Laboratory Animals Monitoring Institute

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Feng Cong

Guangdong Laboratory Animals Monitoring Institute

Miaoli Wu

Guangdong Laboratory Animals Monitoring Institute

Yuexiao Lian

Guangdong Laboratory Animals Monitoring Institute

Bihong Huang

International Monetary Fund

Ren Huang

Shenzhen University

Pengju Guo

Guangdong Laboratory Animals Monitoring Institute

Li Xiao

Nantong University

Meili Chen

Guangdong Laboratory Animals Monitoring Institute

Pengju Guo

Boehringer Ingelheim (China)

Fengjiao Xu

Zhejiang University

Cooperative Institutions

Sun Yat-sen University

Guangdong Laboratory Animals Monitoring Institute

Zhejiang University

Southern University of Science and Technology

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

South China Agricultural University

Chinese Academy of Agricultural Sciences

Nanjing Agricultural University

Wuhan University

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Point‐of‐care diagnostic assay for rapid detection of porcine deltacoronavirus using the recombinase polymerase amplification method

Transboundary and Emerging Diseases (2019)

Lei Ma Fanwen Zeng Bihong Huang Yujun Zhu Miaoli Wu

Porcine deltacoronavirus (PDCoV) has emerged and spread throughout the porcine industry in many countries over the last 6 years. PDCoV caused watery diarrhoea, vomiting and dehydration in newborn piglets. A sensitive diagnostic method would be beneficial to the prevention and control of PDCoV infection. Recombinase polymerase amplification (RPA) is an isothermal amplification method which has been widely used for virus detection. A probe-based reverse transcription RPA (RT-RPA) assay was developed for real-time detection of PDCoV. The amplification can be finished in 20 min and fluorescence monitoring was performed by a portable device. The lowest detection limit of the PDCoV RT-RPA assay was 100 copies of RNA molecules per reaction; moreover, the RT-RPA assay had no cross-reaction with other common swine viruses. The clinical performance of the RT-RPA assay was evaluated using 108 clinical samples (54 intestine specimens and 54 faecal swab specimens). The coincidence rate of the detection results for clinical samples between RT-RPA and RT-qPCR was 97.2%. In summary, the real-time RT-RPA assay offers a promising alternative to RT-qPCR for point-of-care detection of PDCoV.

Recombinase Polymerase Amplification

Point-of-Care Testing

Point of care

10.1111/tbed.13155

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Citations (42)

Effect of groove angle on residual stress and deformation of super duplex stainless steel SAF 2507 and austenitic stainless steel 316L dissimilar plasma arc welding

Materialwissenschaft und Werkstofftechnik (2024)

Hong Xu Bensheng Huang Tianshu Li Junjie Zheng Yujun Zhu

Abstract Plasma arc welding of super duplex stainless steel SAF 2507 and austenitic stainless steel 316L was carried out by using V‐grooves with different groove angles. In order to predict the temperature distribution during the welding process, the Simufact Welding finite element software was used to establish an equivalent heat source to simulate the plasma arc welding process. The equivalent heat source consists of Goldak heat source and conical heat source. In mechanical simulation, the elastoplastic behavior with isotropic hardening is realized. The numerical results are in good agreement with the experimental results. The results show that with the increase of groove angle, the equivalent stress, transverse residual stress, longitudinal residual stress and overall deformation of SAF 2507/316L dissimilar steel welded joint decrease gradually, and the overall deformation of the joint increases gradually. The groove angle has little influence on the equivalent stress, transverse residual stress and overall deformation, but has great influence on the longitudinal residual stress and Z‐direction deformation. Comprehensive analysis of the stress and deformation results under four groove angles shows that the groove angle is too large or too small, which has a great influence on the stress and deformation of the weldment. The groove angle of 60° is better for SAF 2507/316L dissimilar steel welding.

Groove (engineering)

Duplex (building)

Austenitic stainless steel

10.1002/mawe.202300128

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Citations (1)

Unilateral low-frequency stimulation of central piriform cortex inhibits amygdaloid-kindled seizures in Sprague–Dawley rats

Neuroscience (2007)

Zhengbing Zhuge Yujun Zhu Dang Wu Shengjia Wang L.-Y. Liu

Piriform cortex

Seizure threshold

10.1016/j.neuroscience.2007.02.014

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Citations (61)

Establishment of xMAP for the simultaneous detection of antibodies to Newcastle disease virus and avian influenza virus

Poultry Science (2018)

Huanan Wang Feng Cong Jianchi Guan Li Xiao Yujun Zhu

Using Luminex xMAP (x = analyte, MAP = multi-analyte profiling) technology, a serological method for the simultaneous detection of antibodies to Newcastle disease virus (NDV) and avian influenza virus (AIV) was established. Nano-magnetic beads coated with purified NDV protein and AIV nucleoprotein were incubated with serum samples. Using biotinylated rabbit anti-chicken IgY and streptavidin-R-phycoerythrin, the optical signals measured by a Luminex 200 detection system indicated the quantification of NDV or AIV antibodies in the serum. Specific pathogen-free (SPF) chicken serum was used as a negative control. The Luminex xMAP assay developed in this study demonstrated high specificity as there was no cross-reaction with antibodies to infectious laryngotracheitis virus, infectious bronchitis virus, infectious bursal disease virus, avian leukosis virus, and Marek's disease virus. The results from reproducibility experiments showed that intra-coefficients of variation were 3.36 and 9.23% and inter-coefficients of variation were 6.50 and 7.66% for NDV and AIV, respectively. The results also indicated that the Luminex xMAP assay was 16 times more sensitive for NDV antibody detection and 1,024 times more sensitive for AIV antibody detection compared to the enzyme-linked immunosorbent assay (ELISA). A total of 300 chicken serum samples were subjected to both Luminex xMAP assay and ELISA, showing the coincidence rates of 98.67 and 98% for NDV and AIV antibody detection, respectively. This study provides a new method for the simultaneous detection NDV and AIV antibodies in the serum with high specificity and sensitivity.

Newcastle Disease

Infectious bursal disease

10.3382/ps/pey510

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The adenosine analog prodrug ATV006 is orally bioavailable and has preclinical efficacy against parental SARS-CoV-2 and variants

Science Translational Medicine (2022)

Liu Cao Yingjun Li Sidi Yang Guanguan Li Qifan Zhou

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus driving the ongoing coronavirus disease 2019 (COVID-19) pandemic, continues to rapidly evolve. Because of the limited efficacy of vaccination in prevention of SARS-CoV-2 transmission and continuous emergence of variants of concern (VOCs), orally bioavailable and broadly efficacious antiviral drugs are urgently needed. Previously, we showed that the parent nucleoside of remdesivir, GS-441524, has potent anti-SARS-CoV-2 activity. Here, we report that esterification of the 5'-hydroxyl moieties of GS-441524 markedly improved antiviral potency. This 5'-hydroxyl-isobutyryl prodrug, ATV006, demonstrated excellent oral bioavailability in rats and cynomolgus monkeys and exhibited potent antiviral efficacy against different SARS-CoV-2 VOCs in vitro and in three mouse models. Oral administration of ATV006 reduced viral loads and alleviated lung damage when administered prophylactically and therapeutically to K18-hACE2 mice challenged with the Delta variant of SARS-CoV-2. These data indicate that ATV006 represents a promising oral antiviral drug candidate for SARS-CoV-2.

10.1126/scitranslmed.abm7621

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Citations (29)

Simultaneous detection of 4 prototypic rat parvoviruses using the luminex xTAG assay in laboratory animal health monitoring

Journal of Virological Methods (2017)

Fengjiao Xu Wen Yuan Tongyuan Zhang Yujun Zhu Yuexiao Lian

Agarose gel electrophoresis

Multiplex

Agarose

10.1016/j.jviromet.2017.05.017

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Citations (6)

Rapid detection of goose astrovirus genotypes 2 using real-timereverse transcription recombinase polymerase amplification

Research Square (Research Square) (2023)

Haiqin Li Yujun Zhu Chunhe Wan Zhangzhang Wang Lei Liu

Abstract Background Goose astrovirus (GoAstV) is an important pathogen that causes joint and visceral gout in goslings. It has been circulating in many provinces of China since 2017. Goose astrovirus genotypes 2 (GoAstV-2) is the main epidemic strain, and its high morbidity and mortality have caused huge economic losses to the goose industry. An accurate point-of-care detection for GoAstV-2 is of great significance. In this study, we developed a real-time reverse transcription recombinase polymerase amplification (RT-RPA) method for the on-site detection of GoAstV-2 infection. Results The real-time RT-RPA reaction was carried out at a constant temperature of 39°C, and the entire detection time from nucleic acid preparation to the end of amplification was only 25 min using the portable device. The results of a specificity analysis showed that no cross-reaction was observed with other related pathogens. The detection limit of the assay was 100 RNA copies/µL. The low coefficient of variation value indicated excellent repeatability. We used 270 clinical samples to evaluate the performance of our established method, the positive concordance rates with RT-qPCR were 99.6%, and the linear regression analysis revealed a strong correlation. Conclusions The established real-time RT-RPA assay showed high rapidity, specificity and sensitivity, which can be widely applied in the laboratory, field and especially in the resource-limited settings for GoAstV-2 point-of-care diagnosis.

Recombinase Polymerase Amplification

10.21203/rs.3.rs-2449419/v1

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Citations (0)

Correction: Rapid detection of goose astrovirus genotypes 2 using real-time reverse transcription recombinase polymerase amplification

BMC Veterinary Research (2023)

Haiqin Li Yujun Zhu Chunhe Wan Zhangzhang Wang Lei Liu

Recombinase Polymerase Amplification

10.1186/s12917-023-03818-7

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The establishment of a recombinase polymerase amplification technique for the detection of mouse poxvirus

BMC Veterinary Research (2023)

Yuexiao Lian Mengdi Zhang Yujun Zhu Miaoli Wu Bihong Huang

Abstract Background Ectromelia virus (ECTV) is the causative agent of mousepox in mice. In the past century, ECTV was a serious threat to laboratory mouse colonies worldwide. Recombinase polymerase amplification (RPA), which is widely used in virus detection, is an isothermal amplification method. Results In this study, a probe-based RPA detection method was established for rapid and sensitive detection of ECTV.Primers were designed for the highly conserved region of the crmD gene, the main core protein of recessive poxvirus, and standard plasmids were constructed. The lowest detection limit of the ECTV RT- RPA assay was 100 copies of DNA mol-ecules per reaction. In addition, the method showed high specificity and did not cross-react with other common mouse viruses.Therefore, the practicability of the RPA method in the field was confirmed by the detection of 135 clinical samples. The real-time RPA assay was very similar to the ECTV real-time PCR assay, with 100% agreement. Conclusions In conclusion, this RPA assay offers a novel alternative for the simple, sensitive, and specific identification of ECTV, especially in low-resource settings.

Recombinase Polymerase Amplification

Ectromelia virus

Poxviridae

10.1186/s12917-023-03703-3

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Citations (1)

The adenosine analogue prodrug ATV006 is orally bioavailable and has potent preclinical efficacy against SARS-CoV-2 and its variants

bioRxiv (Cold Spring Harbor Laboratory) (2021)

Liu Cao Yingjun Li Sidi Yang Guanguan Li Qifan Zhou

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes the COVID-19 pandemic, is rapidly evolving. Due to the limited efficacy of vaccination in prevention of SARS-CoV-2 transmission and continuous emergence of variants of concern (VOC), including the currently most prevalent Delta variant, orally bioavailable and broadly efficacious antiviral drugs are urgently needed. Previously we showed that adenosine analogue 69-0 (also known as GS-441524), possesses potent anti-SARS-CoV-2 activity. Herein, we report that esterification of the 5’-hydroxyl moieties of 69-0 markedly improved the antiviral potency. The 5’-hydroxyl-isobutyryl prodrug, ATV006, showed excellent oral bioavailability in rats and cynomolgus monkeys and potent antiviral efficacy against different VOCs of SARS-CoV-2 in cell culture and three mouse models. Oral administration of ATV006 significantly reduced viral loads, alleviated lung damage and rescued mice from death in the K18-hACE2 mouse model challenged with the Delta variant. Moreover, ATV006 showed broad antiviral efficacy against different mammal-infecting coronaviruses. These indicate that ATV006 represents a promising oral drug candidate against SARS-CoV-2 VOCs and other coronaviruses.

10.1101/2021.10.13.463130

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Citations (4)