[Objective] To investigate the prevalence of strains producing extended spectrum β-lactamase(ESBLs)in Escherichia coli,and supply the laboratory evidence for antibiotic rational application in clinic.[Methods]VITEK32-automatic identification system was used to identify the bacterium.The drug susceptibility was tested with the K-B method and the ESBLs producing strains detected by disconfirmed test.[Results]141 strains of Escherichia coli were detected and the Positive rate of ESBLs-producing E.coli was 38.1%.E.coli was sensitive to cefoxitin,imipenem,cefoperazone/sulbactam,and the antibiotic resistances were less than 20%.E.coli was resistance to ampicillin,cefazolin,cefotaxime,ceftriaxone,ciprofloxacin,levofloxacin and the antibiotic resistances were all over 50%.The resistance of 14 kinds of commonly used antibiotic in E.coli producing ESBLs was higher than that in E.coli on-producing ESBLs.[Conclusion]The antibiotic resistance of E.coli isolated from clinic of urinary tract infection was increased.Clinical antibiotic use should accord to the AST results.We must pay more attention to ESBLs-producing E.coli.
OBJECTIVE To investigate the antimicrobial resistance and distribution of biovar of clinical isolates of Ureaplasma urealyticum(Uu)from urogenitory tracts of women so as to provide guidance for reasonable clinical use of antibiotics.METHODS By means of drug susceptibility testing kit of BioMerieux,France for the culture,identification,and counting of Mycoplasma,the drug susceptibility testing was performed for Uu cultured positive from 334specimens,then the PCR gene amplification method was employed to identify the biotypes of 60clinical isolates of Uu.RESULTS The drug resistance rates of the 334clinical isolates of Uu to pristinamycin,doxycycline,and tetracycline were 1.20%,8.98%,and 7.49%,respectively;the drug resistance rates to ciprofloxacin and ofloxacin were 83.83%and 56.29%,respectively.Of totally 60clinical isolates of Uu,there were 41(68.33%) strains of biovar Parvo and 19(31.67%)strains of biovar T960.CONCLUSION Doxycycline,josamycin,and clarythromycin can be used as the preferred antibiotics for the clinical treatment of Uu infections,and the antibiotics should be used reasonably based on the result of drug susceptibility testing to delay the emergence of drug resistant strains.The Parvo is the predominant biovar of Uu from female patients of the outpatient department.
Objective To investigate the infection and drug resistance of Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) in Zhengzhou. Methods 13 331 patients were collected in our hospital from July 2012 to June 2013 for Uu and Mh detection. All patients were divided into ≤ 18 age group, 19~30age group, 31~40 age group, 41~50 age group, 51~60 age group and ≥ 61 age group. Uu, Mh and Uu+Mh of all patients were detected, and drug resistance were analyzed. Results The Mycoplasma infection rate in 13331 samples was 50.57%(6741 / 13 331), and the highest constituent ratio was Uu(85.85%, 5787 / 6741). The difference of Uu infection rates and the mixed infection rates among different ages groups were significant(Pall0.05), while the difference of Mh infection rates among different ages groups was not significant. The highest positive rate of Uu was 19 ~30 age group(45.10%), the highest positive rate of Mh was ≥ 61 age group(1.52%), and the highest rate of Uu +Mh was 51 ~60 age group(9.31%). Compared with Mh and mixed infection, drug resistance of Uu was less serious. Tetracyclines antibiotic sensitive rate for Mycoplasma were more than 90%. Excepted josamycin, erythromycins were sensitive for Uu but not for Mh. Quinolones were resistant in varying degrees for Uu and Mh and antibiotic screening test should be done. Conclusion The infection rate of Mycoplasma in the people who likely had urogenital tract infection is 50.57% in Zhengzhou. Uu infection is distributed mainly from 19~50 age and the mixed infection is distributed mainly from 41~60 age.For treatment, we can use Tetracyclines as the first choice drug in this area. At the same time, antibiotic screening test also should be done for drug resistance control.
Objective To investigate the infection and drug resistance of urogenital tract mycoplasma in Zhengzhou male population. Methods The infection and drug resistance of mycoplasma were analyzed in 2 597 samples of urogenital tract. These samples were from the males at the third affiliated hospital of zhengzhou university from July 2012 to June 2013. Results The infection rates of mycoplasma were 30. 07%(781 /2597),in which UU accounted for 88. 99%(695 /781),mixed infection of UU and MH accounted for9. 86%(77 /781) and MH accounted for 1. 15%(9 /781). The male positive rates for mycoplasma were lower in reproductive clinic than other clinics[26. 91%(434 /1 613),vs. 35. 26%(347 /984)]. Compared with mixed and MH infection alone,UU infection exhibited less drug resistance. The majority of mycoplasma were sensitive to tetracyclines,which could be the first choice for mycoplasma. Conclusion In reproductive clinic,one third of the males are mycoplasma infection,mainly UU. Tetracyclines can be the first choice for mycoplasma infection. During the treatment,drugs should be changes according antibiotic sensitive test.
