Inaudis Cejas

Universidad de Ciego de Ávila

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Marcos Edel Martínez-Montero

Universidad de Ciego de Ávila

José Carlos Lorenzo

Universidad de Ciego de Ávila

C. Aragón

Universidad Publica de Navarra

Maritza Escalona

Universidad de Ciego de Ávila

Byron E. Zevallos–Bravo

Universidad Estatal del Sur de Manabi

Lourdes Yabor

Universidad de Ciego de Ávila

Iris Capote

Universidad de Ciego de Ávila

Justo González-Olmedo

Universidad de Ciego de Ávila

Pierre Debergh

Ghent University

Florent Engelmann

Agropolis International

Cooperative Institutions

Universidad de Ciego de Ávila

Universidad de Oviedo

Consejo Superior de Investigaciones Científicas

Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria

Ghent University

Universitat Politècnica de València

University of KwaZulu-Natal

University of Córdoba

University of Naples Federico II

Federico II University Hospital

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Field performance of micropropagated and cryopreserved shoot tips-derived pineapple plants grown in the field for 14 months

Acta Physiologiae Plantarum (2019)

Ariel Villalobos-Olivera Julia Martínez Nicolás Quintana Byron E. Zevallos–Bravo Inaudis Cejas

Germ plasm

Plant Physiology

Liquid nitrogen

10.1007/s11738-019-2825-x

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Citations (9)

Biochemical characterization of ecuadorian wild Solanum lycopersicum Mill. plants produced from non-cryopreserved and cryopreserved seeds.

PubMed (2013)

Byron E. Zevallos–Bravo Inaudis Cejas René C. Rodríguez-Escriba Lourdes Yabor C. Aragón

This paper presents some of the effects of cryopreservation of wild Solanum lycopersicum Mill. seeds on the early stages of germination post liquid nitrogen exposure. Percentage of germination, conversion into plantlets and plant fresh mass were evaluated after cryostorage. Levels of chlorophyll pigments (a, b, total), malondialdehyde, other aldehydes, phenolics (cell wall-linked, free, and total) and proteins were determined. Peroxidase and superoxide dismutase activities were recorded. Liquid nitrogen exposure increased the percentage of seed germination at 5 days but at 7 days, the conversion into plantlets and the plant fresh mass were not statistically different between non-cryopreserved and cryopreserved samples. Several significant effects of cryopreservation were recorded at the biochemical level at 7 days of germination under controlled conditions. Highly significant effects due to liquid nitrogen exposure were observed in leaves: increased levels of peroxidase enzymatic and specific activities and cell wall-linked phenolics. Very remarkable effects were also recorded in roots: decreased contents of chlorophylls and cell wall-linked phenolics.

Liquid nitrogen

Malondialdehyde

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Evaluación del efecto de las condiciones generadas por Biorreactores de inmersión temporal sobre enzimas y procesos clave del metabolismo del carbono en plantas in vitro de plátano cv. CEMSA

Biotecnología vegetal (2004)

C. Aragón Maritza Escalona Iris Capote Danilo Pina Inaudis Cejas

The technique of Temporary Immersion constitutes an effective tool for the plant propagation; it increases the multiplication coefficient and the quality of the same ones. Little it is known until the moment about the echophysiology of this new cultivation technique where the plants are subjected to an immersion between liquid and the physiologic changes that take place during this stage will be those responsible for the plants quality. To evaluate the effects of these conditions in the physiologic change of plantain plants, a descriptive experiment was directed during the elongation phase. Enzymes related with the carbon metabolism were measure of the plant heterotrophic behaviour during the studied phase. High levels of acid invertase (AI) activities and pyruvato kinase (PK) accompanied by first floor levels of sucrose phosphate synthase (SPS) and phosphoenolpyruvate carboxylase (PEPC), evidence these behaviours. The changes induced by the atmosphere and the indicative net photosynthesis and transpiration demonstrate the tendency from the plants to be a less autotrophy systems. The plants during the in vitro conditions don’t depend totally on the photosynthesis but rather they present photomixotrophic behaviour. Key words: acid invertase (AI), Phosphoenolpyruvate carboxylase (PEPC), pyruvato kinase (PK), sucrose phosphate synthase (SPS)

Phosphoenolpyruvate carboxylase

Sucrose-phosphate synthase

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Citations (2)

Photosynthesis and carbon metabolism in plantain (Musa AAB) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization

In Vitro Cellular & Developmental Biology - Plant (2005)

C. Aragón Maritza Escalona Iris Capote Danilo Pina Inaudis Cejas

Phosphoenolpyruvate carboxylase

Sucrose-phosphate synthase

10.1079/ivp2005640

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Citations (41)

Evaluation of the effect of the conditions generated by temporal immersion bioreactors on enzymes and key processes of the carbon metabolism in vitro plants of plantain cv. CEMSA ¾.

DOAJ (DOAJ: Directory of Open Access Journals) (2004)

C. Aragón Maritza Escalona Iris Capote Danilo Pina Inaudis Cejas

Immersion

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Seed Cryostorage Enhances Subsequent Plant Productivity in the Forage Species Teramnus Labialis (L.F.) Spreng.

PubMed (2019)

Yanier Acosta Lázaro Hernández Carlos Mazorra Nicolás Quintana Byron E. Zevallos–Bravo

Teramnus labialis is an herbaceous legume that serves as a source of carbohydrates and proteins for animals and humans, and is valued for its nitrogen contribution to soil. The benefits of this species are, however, limited by low seed availability, small seed size and low in situ seed germination levels, due to physical dormancy. Cryostorage has been shown to be beneficial for both seed storage and breaking physical dormancy in seeds of various species. However, its potential effects on subsequent seedling emergence, plant growth and seed production need to be studied before large-scale implementation for T. labialis.To record agricultural traits of T. labialis after seed exposure to liquid nitrogen.Seeds were maintained at 5 degree C (control) or stored in LN before sowing. Seedling emergence percentage and traits related to plant growth and seed production were evaluated for 6 months.Except for seed weight, all traits differed significantly between seedlings generated from cryostored and control seeds. Except for pod number, seedling emergence and plant growth traits were enhanced by cryostorage to a greater extent than seed production traits. Cryostorage resulted in cracks and breaks in the seed coat which were absent in control seeds (scanning electron microscopy), and in breaking physical dormancy may have facilitated more rapid seedling emergence than for control seeds.Seed cryostorage enhances subsequent plant productivity in terms of growth and to a lesser extent seed production in Teramnus labialis, validating its use for commercial growth of this species.

Seed treatment

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Citations (8)

Effects of Teramnus labialis (L.f.) Spreng seed cryopreservation on subsequent seed and seedling growth and biochemistry

Acta Physiologiae Plantarum (2020)

Yanier Acosta Lianny Pérez Claudia Linares Lázaro Hernández Doris Escalante

Imbibition

Plantlet

Recalcitrant seed

Plant Physiology

10.1007/s11738-020-3012-9

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Citations (13)

Aspectos metabolicos del crecimiento y desarrollo de las plantulas de platano (CEMSA 3/4) micropropagadas en Biorreactores de Inmersion Temporal (BIT)

Cultivos Tropicales (2006)

C. Aragón Maritza Escalona Iris Capote Inaudis Cejas Roberto Rodríguez Madrera

Resumen es: Las tecnicas de cultivo en Biorreactores de Inmersion Temporal (BIT) constituyen una herramienta eficaz para la propagacion de plantulas, pues aumenta el...

Bit (key)

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Citations (5)

Metabolic importance of starch in the acclimation of plantain CEMSA 3/4 (AAB) plants.

Infomusa (2006)

Carlos Eduardo Aragón Abreu Maritza Escalona Iris Capote Danilo Pina Inaudis Cejas

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Citations (4)

Optimización del protocolo de propagación del plátano cv. CEMSA ¾ en Biorreactores de Inmersión Temporal

Agrociencia (2011)

Inaudis Cejas Iris Capote C. Aragón Maritza Escalona Danilo Pina

La automatización de la producción de plantas con el empleo de Biorreactores de Inmersión Temporal (BIT) constituye una herramienta eficaz para la propagación in vitro con la cual se elevan los coeficientes de multiplicación y se aumenta la calidad del material propagado. Algunos factores que influyen en este proceso son las características de los explantes que se emplean para inocular los BIT, la manipulación de los mismos y su estado fisiológico. Estos, aparejados a los indicadores de eficiencia del sistema, son elementos a tener en cuenta en el estudio de la morfogénesis de cultivos como el plátano, que presenta índices de proliferación bajos en medio semisólido. Con el objetivo de optimizar el protocolo descrito por Roels, et al. (2005), se evaluó el efecto del manejo del explante (con división y sin división), el volumen de medio de cultivo durante tres ciclos de proliferación de brotes de plátano cv. CEMSA ¾ sobre el coeficiente de multiplicación y la calidad de los mismos. El mayor coeficiente de multiplicación se logró con el tratamiento en el que se incrementó el volumen del medio de cultivo en función de la biomasa sin seccionar el explante durante los tres ciclos de propagación. Estas modificaciones al protocolo de Roels, et al. (2005), al reducir la manipulación de los explantes durante cada ciclo de cultivo y aumentar las tasas de multiplicación, lo hacen más eficiente y simple, y de esta manera más aplicable para la propagación de plátano a gran escala.

10.31285/agro.15.606

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Citations (4)