Apeksha Damania

Indian Institute of Technology Kanpur

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Ashok Kumar

Indian Institute of Technology Kanpur

Shiv Kumar Sarin

Institute of Liver and Biliary Sciences

Era Jain

Syracuse University

Arun Kumar Teotia

Aalto University

Anupam Kumar

Vanderbilt University Medical Center

Anupam Kumar

Mizoram University

Hiroyuki Ijima

Kyushu University

Masamichi Kamihira

Kyushu University

Mark R. Towler

Toronto Metropolitan University

Jingjue Gong

Smith College

Cooperative Institutions

Smith College

126

Syracuse University

120

University of Memphis

115

Texas A&M University

115

John Wiley & Sons (United States)

110

Louisiana State University Health Sciences Center New Orleans

109

United States Food and Drug Administration

Indian Institute of Technology Kanpur

Center for Devices and Radiological Health

University at Buffalo, State University of New York

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Fabrication of macroporous cryogels as potential hepatocyte carriers for bioartificial liver support

Colloids and Surfaces B Biointerfaces (2015)

Era Jain Apeksha Damania Akhilesh Kumar Shakya Anupam Kumar Shiv Kumar Sarin

Bioartificial liver device

Biocompatibility

10.1016/j.colsurfb.2015.10.012

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Citations (48)

Synthesis and Characterization of Cryogels

CRC Press eBooks (2016)

Apeksha Damania Arun Kumar Teotia Ashok Kumar

Characterization

10.1201/b19676-4

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Citations (3)

Mesenchymal stromal cell-derived exosome-rich fractionated secretome confers a hepatoprotective effect in liver injury

Stem Cell Research & Therapy (2018)

Apeksha Damania Deepika Jaiman Arun Kumar Teotia Ashok Kumar

Mesenchymal stromal cells (MSCs) are an attractive therapeutic agent in regenerative medicine. Recently, there has been a paradigm shift from differentiation of MSCs to their paracrine effects at the injury site. Several reports elucidate the role of trophic factors secreted by MSCs toward the repair of injured tissues. We hypothesize that fractionating the MSC secretome will enrich exosomes containing soluble bioactive molecules, improving its therapeutic potential for liver failure.Rat bone marrow MSCs were isolated and the conditioned media filtered, concentrated and ultracentrifuged to generate fractionated secretome. This secretome was characterized for the presence of exosomes and recovery from liver injury assessed in in-vitro liver injury models. The results were further validated in vivo.Studies on in-vitro liver injury models using acetaminophen and hydrogen peroxide show better cell recovery and reduced cytotoxicity in the presence of fractionated as opposed to unfractionated secretome. Further, the cells showed reduced oxidative stress in the presence of fractionated secretome, suggesting a potential antioxidative effect. These results were further validated in vivo in liver failure models, wherein improved liver regeneration in the presence of fractionated secretome (0.819 ± 0.035) was observed as compared to unfractionated secretome (0.718 ± 0.042).The work presented is a proof of concept that fractionating the secretome enriches certain bioactive molecules involved in the repair and recovery of injured liver tissue. Exosome enriched mesenchymal stromal cell-derived fractionated secretome potentiates recovery upon injection in injured liver.

Exosome

10.1186/s13287-017-0752-6

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Citations (131)

Decellularized Liver Matrix-Modified Cryogel Scaffolds as Potential Hepatocyte Carriers in Bioartificial Liver Support Systems and Implantable Liver Constructs

ACS Applied Materials & Interfaces (2017)

Apeksha Damania Anupam Kumar Arun Kumar Teotia Haruna Kimura Masamichi Kamihira

Recent progress in the use of decellularized organ scaffolds as regenerative matrices for tissue engineering holds great promise in addressing the issue of donor organ shortage. Decellularization preserves the mechanical integrity, composition, and microvasculature critical for zonation of hepatocytes in the liver. Earlier studies have reported the possibility of repopulating decellularized matrices with hepatic cell lines or stem cells to improve liver regeneration. In this work, we study the versatility of the decellularized liver matrix as a substrate coating of three-dimensional cryogel scaffolds. The coated cryogels were analyzed for their ability to maintain hepatic cell growth and functionality in vitro, which was found to be significantly better than the uncoated cryogel scaffolds. The decellularized liver matrix-coated cryogel scaffolds were evaluated for their potential application as a cell-loaded bioreactor for bioartificial liver support and as an implantable liver construct. Extracorporeal connection of the coated cryogel bioreactor to a liver failure model showed improvement in liver function parameters. Additionally, offline clinical evaluation of the bioreactor using patient-derived liver failure plasma showed its efficacy in improving liver failure conditions by approximately 30-60%. Furthermore, implantation of the decellularized matrix-coated cryogel showed complete integration with the native tissue as confirmed by hematoxylin and eosin staining of tissue sections. HepG2 cells and primary human hepatocytes seeded in the coated cryogel scaffolds implanted in the liver failure model maintained functionality in terms of albumin synthesis and cytochrome P450 activity post 2 weeks of implantation. In addition, a 20-60% improvement in liver function parameters was observed post implantation. These results, put together, suggest a possibility of using the decellularized matrix-coated cryogel scaffolds for liver tissue engineering applications.

Bioartificial liver device

Decellularization

Artificial liver

Matrix (chemical analysis)

10.1021/acsami.7b13727

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Citations (64)

Enhancing patient outcomes: the role of clinical utility in guiding healthcare providers in curating radiology AI applications

Frontiers in Digital Health (2024)

Franziska Lobig Jacob L. Graham Apeksha Damania Brian Sattin Joana Reis

With advancements in artificial intelligence (AI) dominating the headlines, diagnostic imaging radiology is no exception to the accelerating role that AI is playing in today's technology landscape. The number of AI-driven radiology diagnostic imaging applications (digital diagnostics) that are both commercially available and in-development is rapidly expanding as are the potential benefits these tools can deliver for patients and providers alike. Healthcare providers seeking to harness the potential benefits of digital diagnostics may consider evaluating these tools and their corresponding use cases in a systematic and structured manner to ensure optimal capital deployment, resource utilization, and, ultimately, patient outcomes—or clinical utility. We propose several guiding themes when using clinical utility to curate digital diagnostics.

10.3389/fdgth.2024.1359383

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Advancements in in vitro hepatic models: application for drug screening and therapeutics

Hepatology International (2013)

Apeksha Damania Era Jain Ashok Kumar

Decellularization

Matrigel

Hepatic stellate cell

Liver cell

10.1007/s12072-013-9490-8

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Citations (17)

Issue Information

Journal of Biomedical Materials Research Part B Applied Biomaterials (2017)

Jeremy L. Gilbert Joel D. Bumgardner Elizabeth Cosgriff‐Hernandez Jean T. Jacob Akinori Moroi

Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices.It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used.Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano-and biomimetic-biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products.

10.1002/jbm.b.33822

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Alleviating liver failure conditions using an integrated hybrid cryogel based cellular bioreactor as a bioartificial liver support

Scientific Reports (2017)

Apeksha Damania Mohsin Hassan Nana Shirakigawa Hiroshi Mizumoto Anupam Kumar

Abstract Conventionally, some bioartificial liver devices are used with separate plasmapheresis unit to separate out plasma from whole blood and adsorbent column to detoxify plasma before it passes through a hepatocytes-laden bioreactor. We aim to develop a hybrid bioreactor that integrates the separate modules in one compact design improving the efficacy of the cryogel based bioreactor as a bioartificial liver support. A plasma separation membrane and an activated carbon cloth are placed over a HepG2-loaded cryogel scaffold in a three-chambered bioreactor design. This bioreactor is consequently connected extracorporeally to a rat model of acute liver failure for 3 h and major biochemical parameters studied. Bilirubin and aspartate transaminase showed a percentage decrease of 20–60% in the integrated bioreactor as opposed to 5–15% in the conventional setup. Urea and ammonia levels which showed negligible change in the conventional setup increase (40%) and decrease (18%), respectively in the integrated system. Also, an overall increase of 5% in human albumin in rat plasma indicated bioreactor functionality in terms of synthetic functions. These results were corroborated by offline evaluation of patient plasma. Hence, integrating the plasmapheresis and adsorbent units with the bioreactor module in one compact design improves the efficacy of the bioartificial liver device.

Bioartificial liver device

Plasmapheresis

10.1038/srep40323

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Citations (16)

Optimized performance of the integrated hepatic cell‐loaded cryogel‐based bioreactor with intermittent perfusion of acute liver failure plasma

Journal of Biomedical Materials Research Part B Applied Biomaterials (2017)

Apeksha Damania Anupam Kumar Shiv Kumar Sarin Ashok Kumar

Acute liver failure (ALF) plasma has cytotoxic effects on the cell-loaded bioreactor in bioartificial liver support systems due to the presence of innumerable hepatotoxic compounds that adversely affect the morphology and functionality of the cells. We have designed a hybrid bioreactor that integrates a hepatic cell-loaded cryogel disc and an activated carbon cloth in one compact unit, with potential application as a bioartificial liver support. In this article, we assess the performance of this integrated hybrid cryogel-based bioreactor in a perfusion-based culture system and analyze its functionality and longevity in the presence of intermittent exposure to ALF plasma. The bioreactor maintained functionality in terms of glucose consumption and albumin synthesis for up to 40 days under perfusion. Additionally, intermittent perfusion of plasma from rodent models of ALF resulted in a decrease in viability and functionality only after the second spike of plasma, with the bioreactor maintaining its functionality even after the first spike. Similar results were obtained with patient plasma indicating the potential to reuse the bioreactor for multiple sessions of liver dialysis. Collectively, these results suggest the potential of the integrated cryogel-based bioreactor to be used at most twice before being disposed of. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 259-269, 2018.

Bioartificial liver device

10.1002/jbm.b.33851

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Citations (7)

Biomaterials for liver tissue engineering

Hepatology International (2013)

Era Jain Apeksha Damania Ashok Kumar

Decellularization

Liver cell

Matrix (chemical analysis)

10.1007/s12072-013-9503-7

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Citations (65)